Utilization of viscoelastic substrates to study actin/MTs coordination and subsequent mechanobiological functionalities
| Project/Area Number |
22KJ3112
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| Project/Area Number (Other) |
21J40229 (2021-2022)
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| Research Category |
Grant-in-Aid for JSPS Fellows
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| Allocation Type | Multi-year Fund (2023)
Single-year Grants (2021-2022) |
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| Section | 国内 |
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| Review Section |
Basic Section 90120:Biomaterials-related
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| Research Institution | National Institute for Materials Science |
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Principal Investigator |
Abdelaleem Shimaa 国立研究開発法人物質・材料研究機構, 高分子・バイオ材料研究センター, 特別研究員(RPD)
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| Project Period (FY) |
2023-03-08 – 2024-03-31
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| Project Status |
Completed (Fiscal Year 2023)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2023: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2022: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2021: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | microtubules / actin / Leader cells / collective migration / Mechanobiology / Viscoelastic material / EMT / Actomyosin / ECM concentration / nanoparticle / AFM |
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| Outline of Research at the Start |
Viscoelastic substrates and cRGD concentrations are used to identify the cytoskeletal systems coordination and crosstalk. These surfaces with diversified biomechanical properties are used to determine the crosstalk and functionalities of actin in the absence and presence of MTs. The visualization of targeted proteins, RNA silencing, transfection and rt-PCR would be utilized to achieve this purpose. Moreover, the establishment of stable cell lines with specific fret sensors would be pursued.
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| Outline of Annual Research Achievements |
Studying biological processes with the help of biomaterials that mimic both mechanical and biochemical cues is an effective way to understand cellular responses, especially those related to the cytoskeletal system. To achieve this goal, we have used cRGD concentration as a biochemical cue to investigate the relationship between MTs and cellular mechanics and morphology. Our study has shown that the contribution of MTs to cellular stiffness and morphology is dependent on the underlying cRGD densities. (These results have been submitted, and the research article is currently being revised.)
In collaborative research, we employed photoactivatable surfaces to explore the influence of confinement, among other environmental stimuli like cellular phenotypes and cytoskeletal system perturbations, on the stress inside living cells using atomic force microscopy. Our results indicate that changes in intracellular prestress occurs in accordance with the environmental stimuli. This study was published STAM 2023 and selected as news research highlight in several websites.
Additionally, the photoactivatable surfaces have been utilized to show the effect of alteration of ECM compositions on the collective cell migration and leader cell formation, we showed a new correlation between leader cells in collective migration and the biochemical signals that arise from underlying extracellular matrix proteins (These results have been submitted, and the research article is currently being revised.; preliminary results were presented at two conferences)
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Report
(3 results)
Research Products
(10 results)
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[Presentation] Cell under Stress: Probing Intracellular Force Dynamics using Atomic Force Microscope2022
Author(s)
H. Wang, H. Zhang, R. Tamura, B. Da, S. A. Abdellatef, I. Watanabe, N. Ishida, D. Fujita, N. Hanagata, T. Nakagawa, J. Nakanishi
Organizer
NIMS week
Related Report
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