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Establishment of high-throughput screening of toxic chemical compounds.

Research Project

Project/Area Number 23221005
Research Category

Grant-in-Aid for Scientific Research (S)

Allocation TypeSingle-year Grants
Research Field Risk sciences of radiation/Chemicals
Research InstitutionKyoto University

Principal Investigator

Takeda Shunichi  京都大学, 医学(系)研究科(研究院), 教授 (60188191)

Co-Investigator(Kenkyū-buntansha) HIROTA Kouji  首都大学東京, 大学院理工学研究科, 教授 (00342840)
YAMADA Ryo  京都大学, 大学院医学研究科, 教授 (50301106)
OKADA Tetsuya  京都大学, 大学院理学研究科, 助教 (70378529)
SASANUMA Hiroyuki  京都大学, 大学院医学研究科, 准教授 (00531691)
清水 宏泰  大阪医科大学, 医学部, 准教授 (60340551)
Co-Investigator(Renkei-kenkyūsha) SHIMIZU Hiroyasu  大阪医科大学, 医学部, 准教授 (60340551)
TAKAHASHI Ryosuke  京都大学, 大学院医学研究科, 教授 (90216771)
Project Period (FY) 2011-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥214,890,000 (Direct Cost: ¥165,300,000、Indirect Cost: ¥49,590,000)
Fiscal Year 2015: ¥37,700,000 (Direct Cost: ¥29,000,000、Indirect Cost: ¥8,700,000)
Fiscal Year 2014: ¥47,320,000 (Direct Cost: ¥36,400,000、Indirect Cost: ¥10,920,000)
Fiscal Year 2013: ¥46,280,000 (Direct Cost: ¥35,600,000、Indirect Cost: ¥10,680,000)
Fiscal Year 2012: ¥40,040,000 (Direct Cost: ¥30,800,000、Indirect Cost: ¥9,240,000)
Fiscal Year 2011: ¥43,550,000 (Direct Cost: ¥33,500,000、Indirect Cost: ¥10,050,000)
Keywords有害化学物質 / 化審法 / 遺伝毒物学 / 発がん物質 / 変異原 / 小核実験 / ハイスループットスクリーニング / レギュラトリーサイエンス / 小核試験 / はいスループットスクリーニング / 情報学 / データマイニング / DT40細胞
Outline of Final Research Achievements

Isogenic chicken DT40 B-lymphocyte cell lines deficient in DNA repair pathways can be used to identify genotoxic compounds. As part of the US Tox21 program, we optimised several different DT40 isogenic clones on a high-throughput screening platform and confirmed the utility of this approach for detecting genotoxicants by measuring differential cytotoxicity in wild-type and DNA repair-deficient clones following chemical exposure. We then screened the Tox21 10K golden-standard chemical compound library, and demonstrate the utility of this chemical-genetic approach for identifying and prioritising compounds that may damage DNA.
To improve the bioassay of detecting genotoxicants, we established a method of disrupting genes in the human TK6 B cell line, a standard cell line for identifying genotoxicants by OECD countries. We have already generated ~20 TK6 mutants dedficient in various DNA-repair-genes.

Assessment Rating
Verification Result (Rating)

A

Assessment Rating
Result (Rating)

A+: Progress in the research exceeds the initial goal. More than expected research results are expected.

Report

(9 results)
  • 2016 Research Progress Assessment (Verification Result) ( PDF )
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report   Abstract(Research Progress Assessment) ( PDF )   Research Progress Assessment (Result) ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (148 results)

All 2016 2015 2014 2013 2012 2011 Other

All Int'l Joint Research (2 results) Journal Article (68 results) (of which Int'l Joint Research: 7 results,  Peer Reviewed: 68 results,  Open Access: 24 results,  Acknowledgement Compliant: 12 results) Presentation (71 results) (of which Int'l Joint Research: 10 results,  Invited: 11 results) Book (3 results) Remarks (4 results)

  • [Int'l Joint Research] National Institutes of Health(米国)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] MRC分子生物学研究所(英国)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] The role of HERC2 and RNF8 ubiquitin E3 ligases in the promotion of translesion DNA synthesis in the chicken DT40 cell line.2016

    • Author(s)
      Mohiuddin, Kobayashi S, Keka IS, Guilbaud G, Sale J, Narita T, Abdel-Aziz HI, Wang X, Ogawa S, Sasanuma H, Chiu R, Oestergaard VH, Lisby M, Takeda S.
    • Journal Title

      DNA Repair (Amst)

      Volume: 40 Pages: 64-76

    • DOI

      10.1016/j.dnarep.2016.02.002

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Identification of genotoxic compounds using isogenic DNA repair deficient DT40 cell lines on a quantitative high throughput screening platform.2016

    • Author(s)
      Nishihara K, Huang R, Zhao J, Shahane SA, Witt SK, Smith-Roe SL, Tice RR, Takeda S, Xia M.
    • Journal Title

      Mutagenesis

      Volume: 31 Pages: 69-81

    • DOI

      10.1093/mutage/gev055

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Local potentiation of stress-responsive genes by upstream noncoding transcription.2016

    • Author(s)
      Takemata N, Oda A, Yamada T, Galipon J, Miyoshi T, Suzuki Y, Sugano S, Hoffman CS, Hirota K, Ohta K.
    • Journal Title

      Nucleic Acids Res.

      Volume: in press

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Synergistic association of elevated serum free fatty acid and glucose levels with large arterial stiffness in a general population: The Nagahama Study2016

    • Author(s)
      Yasuharu Tabara, Yoshimitsu Takahashi, Kazuya Setoh, Takahisa Kawaguchi, Norimoto Gotoh, Chikashi Terao, Ryo Yamada, Shinji Kosugi, Akihiro Sekine, Takeo Nakayama, Fumihiko Matsuda, on behalf of the Nagahama Study group.
    • Journal Title

      M E T A B O L I S M C L I N I C A L A N D E X P E R I M E N T A L

      Volume: 65 Issue: 1 Pages: 66-72

    • DOI

      10.1016/j.metabol.2015.10.011

    • NAID

      120005698009

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Abacavir, an anti–HIV-1 drug, targets TDP1-deficient adult T cell leukemia.2015

    • Author(s)
      Tada K, Kobayashi M, Takiuchi Y, Iwai F, Sakamoto T, Nagata K, Shinohara M, Io K, Shirakawa K, Hishizawa M, Shindo K, Kadowaki N, Hirota K, Yamamoto J, Iwai S, Sasanuma H, Takeda S, Takaori-Kondo A.
    • Journal Title

      Science Advances

      Volume: 1 Issue: 3 Pages: 1-9

    • DOI

      10.1126/sciadv.1400203

    • NAID

      120005601014

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Forcible destruction of severely misfolded mammalian glycoproteins by the non-glycoprotein ERAD pathway.2015

    • Author(s)
      Ninagawa S, Okada T, Sumitomo Y, Horimoto S, Sugimoto T, Ishikawa T, Takeda S, Yamamoto T, Suzuki T, Kamiya Y, Kato K, Mori K.
    • Journal Title

      The Journal of Cellular Biology

      Volume: 211 Issue: 4 Pages: 775-784

    • DOI

      10.1083/jcb.201504109

    • NAID

      120005672576

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Cytotoxic and genotoxic profiles of benzo[a]pyrene and N-nitrosodimethylamine demonstrated using DNA repair deficient DT40 cells with metabolic activation.2015

    • Author(s)
      Ooka M, Takazawa H, Takeda S, Hirota K.
    • Journal Title

      Chemosphere

      Volume: 144 Pages: 1901-1907

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Relative contribution of four nucleases, CtIP, Dna2, Exo1 and Mre11, to the initial step of DNA double‐strand break repair by homologous recombination in both the chicken DT40 and human TK6 cell lines2015

    • Author(s)
      Hoa NN, Akagawa R, Yamasaki T, Hirota K, Sasa K, Natsume T, Kobayashi J, Sakuma T, Yamamoto T, Komatsu K, Kanemaki MT, Pommier Y, Takeda S, Sasanuma H
    • Journal Title

      Genes to Cells

      Volume: 20 Issue: 12 Pages: 1059-1076

    • DOI

      10.1111/gtc.12310

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Distinct DNA damage spectra induced by ionizing radiation in normoxic and hypoxic cells.2015

    • Author(s)
      Shimizu N, Ooka M, Takagi T, Takeda S, Hirota K.
    • Journal Title

      Radiation Res

      Volume: 184(4) Issue: 4 Pages: 442-8

    • DOI

      10.1667/rr14117.1

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Smarcal1 promotes double-strand-break repair by nonhomologous end-joining2015

    • Author(s)
      Keka IS, Mohiuddin, Maede Y, Rahman MM, Sakuma T, Honma M, Yamamoto T, Takeda S, Sasanuma H
    • Journal Title

      Nucleic Acid Research

      Volume: 13 Issue: 13 Pages: 6259-6372

    • DOI

      10.1093/nar/gkv621

    • NAID

      120005617590

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Production of Extrachromosomal MicroDNAs Is Linked to Mismatch Repair Pathways and Transcriptional Activity.2015

    • Author(s)
      Dillon LW, Kumar P, Shibata Y, Wang YH, Willcox S, Griffith JD, Pommier Y, Takeda S, Dutta A.
    • Journal Title

      Cell Rep.

      Volume: 11 Issue: 11 Pages: 1749-1759

    • DOI

      10.1016/j.celrep.2015.05.020

    • NAID

      120005829370

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] BRCA1 and CtIP are both required to recruit dna2 at double-strand breaks in homologous recombination.2015

    • Author(s)
      Hoa NN, Kobayashi J, Omura M, Hirakawa M, Yang SH, Komatsu K, Paull TT, Takeda S, Sasanuma H.
    • Journal Title

      PLoS One

      Volume: 10 Issue: 4 Pages: e0124495-e0124495

    • DOI

      10.1371/journal.pone.0124495

    • NAID

      120005615636

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Development of a targeted flip-in system in avian DT40 cells.2015

    • Author(s)
      Kobayashi, K., Fujii, T., Asada, R., Ooka, M., Hirota, K.
    • Journal Title

      PLoS One

      Volume: 10(3) Issue: 3 Pages: e0122006-e0122006

    • DOI

      10.1371/journal.pone.0122006

    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Antagonistic controls of chromatin and mrna start site selection by Tup family corepressors and the CCAAT-binding factor.2015

    • Author(s)
      Asada R, Takemata N, Hoffman CS, Ohta K, Hirota K.
    • Journal Title

      Mol Cell Biol.

      Volume: 35 Issue: 5 Pages: 847-855

    • DOI

      10.1128/mcb.00924-14

    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Abacavir, an anti-HIV-1 drug, targets TDP1 deficient adult T-cell leukemia. Science Advances.2015

    • Author(s)
      Kobayashi M, Takeda S, Takaori A, et al.
    • Journal Title

      Sci Adv.

      Volume: in press

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] BRCA1 and CtIP are both required to recruit dna2 at double-strand breaks in homologous recombination.2015

    • Author(s)
      Nguyen NH, Kobayashi J, Omura M, Hirakawa M, Yang SH, Komatsu K, Paull TT, Takeda S, Sasanuma H
    • Journal Title

      PLoS One

      Volume: in press

    • NAID

      120005615636

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Viable neuronopathic Gaucher disease model in medaka (Oryzias latipes) displays axonal accumulation of alpha-synuclein2015

    • Author(s)
      Norihito Uemura, Masato Koike, Satoshi Ansai, Masato Kinoshita, Tomoko Ishikawa-Fujiwara, Hideaki Matsui, Kiyoshi Naruse, Naoaki Sakamoto, Yasuo Uchiyama, Takeshi Todo, Shunichi Takeda, Hodaka Yamakado, Ryosuke Takahashi
    • Journal Title

      PLoS Genetics

      Volume: 11 Issue: 4 Pages: 1-14

    • DOI

      10.1371/journal.pgen.1005065

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The POLD3 subunit of DNA polymerase δ can promote translesion synthesis independently of DNA polymerase ζ.2015

    • Author(s)
      Hirota K, Yoshikiyo K, Guilbaud G, Tsurimoto T, Murai J, Tsuda M, Phillips L, Narita T, Nishihara K, Kobayashi K, Yamada K, Nakamura J, Pommier Y, Lehmann, A, Sale J, Takeda S
    • Journal Title

      Nucleic Acids Res.

      Volume: 43 Issue: 3 Pages: 1671-1683

    • DOI

      10.1093/nar/gkv023

    • NAID

      120005825923

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Rad18 and Rnf8 facilitate homologous recombination by two distinct mechanisms, promoting Rad51 focus formation and suppressing the toxic effect of nonhomologous end-joining.2015

    • Author(s)
      Kobayashi S, Kasaishi Y, Nakada S, Takagi T, Era S, Motegi A, Chiu RK, Takeda S, Hirota K
    • Journal Title

      Oncogene

      Volume: in press

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Isotonic regression-based method in quantitative high-throughput screenings for genotoxicity.2015

    • Author(s)
      Fujii Y, Narita T, Tice RR, Takeda S, Yamada R
    • Journal Title

      Dose Response

      Volume: 1 Pages: 1-20

    • NAID

      120005668062

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] SUMO-targeted ubiquitin ligase RNF4 plays a critical role in preventing chromosome loss.2014

    • Author(s)
      Hirota K, Tsuda M, Murai J, Takagi T, Keka IS, Narita T, Fujita M, Sasanuma H, Kobayashi J, Takeda S
    • Journal Title

      Genes Cells

      Volume: 19 Issue: 10 Pages: 743-754

    • DOI

      10.1111/gtc.12173

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Identification of novel PARP inhibitors using a cell-based TDP1 inhibitory assay in a quantitative high-throughput screening platform.2014

    • Author(s)
      Murai J, Marchand C, Shahane SA, Sun H, Huang R, Zhang Y, Chergui A, Ji J, Doroshow JH, Jadhav A, Takeda S, Xia M, Pommier Y
    • Journal Title

      DNA Repair

      Volume: 21 Pages: 177-182

    • DOI

      10.1016/j.dnarep.2014.03.006

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rationale for PARP inhibitors in combination therapy with camptothecins or temozolomide based on PARP trapping versus catalytic inhibition.2014

    • Author(s)
      Murai J, Zhang Y, Morris J, Ji J, Takeda S, Doroshow J, Pommier YG
    • Journal Title

      J Pharmacol Exp Ther.

      Volume: 349 Issue: 3 Pages: 408-416

    • DOI

      10.1124/jpet.113.210146

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] RNA cytidine acetyltransferase of small-subunit ribosomal RNA: identification of acetylation sites and the responsible acetyltransferase in fission yeast, Schizosaccharomyces pombe.2014

    • Author(s)
      Taoka M, Ishikawa D, Nobe Y, Ishikawa H, Yamauchi Y, Terukina G, Nakayama H, Hirota K, Takahashi N, Isobe T
    • Journal Title

      PLoS One

      Volume: 9 Issue: 11 Pages: e112156-e112156

    • DOI

      10.1371/journal.pone.0112156

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Evolution of Pre-Existing versus Acquired Resistance to Platinum Drugs and PARP Inhibitors in BRCA-Associated Cancers.2014

    • Author(s)
      Kimiyo N.Yamamoto, Hirota Kouji, Shunichi Takeda, and Hiroshi Haeno.
    • Journal Title

      PLos One

      Volume: 9 Issue: 8 Pages: e105724-e105724

    • DOI

      10.1371/journal.pone.0105724

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] RNase MRP cleaves pre-tRNASer-Met in the tRNA maturation pathway.2014

    • Author(s)
      Saito Y, Takeda J, Adachi K, Nobe Y, Kobayashi J, Hirota K, Oliveira DV, Taoka M, Isobe T
    • Journal Title

      PLoS One

      Volume: 9

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A nationwide study of SLE in Japanese identified subgroups of patients with clear signs patterns and associations between signs and age or sex.2014

    • Author(s)
      Terao C, Yamada R, Mimori T, Yamamoto K, Sumida T
    • Journal Title

      Lupus

      Volume: 23 Issue: 13 Pages: 1435-1442

    • DOI

      10.1177/0961203314547790

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] EDEM2 initiates mammalian glycoprotein ERAD by catalyzing the first mannose trimming step.2014

    • Author(s)
      Ninagawa S, Okada T, Sumitomo Y, Kamiya Y, Kato K, Horimoto S, Ishikawa T, Takeda S, Sakuma T, Yamamoto T, Mori K
    • Journal Title

      J Cell Biol.

      Volume: 206 Issue: 3 Pages: 347-356

    • DOI

      10.1083/jcb.201404075

    • NAID

      120005468381

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Rationale for PARP inhibitors in combination therapy with camptothecins or temozolomide based on PARP trapping versus catalytic inhibition.2014

    • Author(s)
      Murai J, Zhang Y, Morris J, Ji J, Takeda S, Doroshow JH, Pommier YG
    • Journal Title

      J Pharmacol Exp Ther.

      Volume: in press

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Berberine induces double-strand DNA breaks in Rev3 deficient cells.2014

    • Author(s)
      Hu X, Wu X, Huang Y, Tong Q, Takeda S, Qing Y
    • Journal Title

      Mol Med Rep.

      Volume: 9 Issue: 5 Pages: 1883-1888

    • DOI

      10.3892/mmr.2014.1999

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Histone deacetylase inhibitors selectively target homology dependent DNA repair defective cells and elevate non-homologous endjoining activity.2014

    • Author(s)
      Smith S, Fox J, Mejia M, Ruangpradit W, Saberi A, Kim S, Choi Y, Oh S, Wang Y, Choi K, Li L, Hendrickson EA, Takeda S, Muller M, Myung K
    • Journal Title

      PLoS One

      Volume: 9 Issue: 1 Pages: e87203-e87203

    • DOI

      10.1371/journal.pone.0087203

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Stereospecific PARP trapping by BMN 673 and comparison with olaparib and rucaparib.2014

    • Author(s)
      Murai J, Huang SY, Renaud A, Zhang Y, Ji J, Takeda S, Morris J, Teicher B, Doroshow JH, Pommier Y
    • Journal Title

      Mol Cancer Ther.

      Volume: 13 Issue: 2 Pages: 433-443

    • DOI

      10.1158/1535-7163.mct-13-0803

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Compensatory functions and interdependency of the DNA-binding domain of BRCA2 with the BRCA1-PALB2-BRCA2 complex.2014

    • Author(s)
      Al Abo M, Dejsuphong D, Hirota K, Yonetani Y, Yamazoe M, Kurumizaka H, Takeda S
    • Journal Title

      Cancer Res.

      Volume: 74 Issue: 3 Pages: 797-807

    • DOI

      10.1158/0008-5472.can-13-1443

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Differential and common DNA repair pathways for topoisomerase I- and II-targeted drugs in a genetic DT40 repair cell screen panel.2014

    • Author(s)
      Maede Y, Shimizu H, Fukushima T, Kogame T, Nakamura T, Miki T, Takeda S, Pommier Y, Murai J
    • Journal Title

      Mol Cancer Ther.

      Volume: 13 Issue: 1 Pages: 214-220

    • DOI

      10.1158/1535-7163.mct-13-0551

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A novel genotoxicity assay of carbon nanotubes using functional macrophage receptor with collagenous structure (MARCO)-expressing chicken B lymphocytes.2014

    • Author(s)
      Mohiuddin, Keka IS, Evans TJ, Hirota K, Shimizu H, Kono K, Takeda S, Hirano S
    • Journal Title

      Arch Toxicol.

      Volume: 88 Issue: 1 Pages: 145-160

    • DOI

      10.1007/s00204-013-1084-7

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] C-terminal region of activation-induced cytidine deaminase (AID) is required for efficient class switch recombination and gene conversion.2014

    • Author(s)
      Sabouri S, Kobayashi M, Begum NA, Xu J, Hirota K, Honjo T
    • Journal Title

      Proc Natl Acad Sci U S A.

      Volume: 111 Issue: 6 Pages: 2253-2258

    • DOI

      10.1073/pnas.1324057111

    • NAID

      120005395696

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Isotonic regression-based method in quantitative hith-throughput screenings for genotoxicity.2014

    • Author(s)
      Fujii Y, Narita T, Tice R, Takeda S, Yamada R
    • Journal Title

      Dose Response.

      Volume: in press

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genetics of rheumatoid arthritis contributes to biology and drug discovery.2014

    • Author(s)
      Okada Y, Wu D, Trynka G, Raj T, Terao C, Ikari K, Kochi Y, Ohmura K, Suzuki A, Yoshida S, Graham RR, Manoharan A, Ortmann W, Bhangale T, Denny JC, (省略), GARNET consortium, (以下省略).
    • Journal Title

      Nature

      Volume: 506 Issue: 7488 Pages: 376-381

    • DOI

      10.1038/nature12873

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Chicken DT40 cell line lacking DJ-1, the gene responsible for familial Parkinson's disease, displays mitochondrial dysfunction.2013

    • Author(s)
      Minakawa EN, Yamakado H, Tanaka A, Uemura K, Takeda S, Takahashi R
    • Journal Title

      Neurosci Res.

      Volume: 77 Issue: 4 Pages: 228-233

    • DOI

      10.1016/j.neures.2013.09.006

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genotoxic potentials and related mechanisms of bisphenol A and other bisphenol compounds: a comparison study employing chicken DT40 cells.2013

    • Author(s)
      Lee S, Liu X, Takeda S, Choi K
    • Journal Title

      Chemosphere

      Volume: 93 Issue: 2 Pages: 434-440

    • DOI

      10.1016/j.chemosphere.2013.05.029

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The proteasome factor Bag101 binds to Rad22 and suppresses homologous recombination.2013

    • Author(s)
      Saito Y, Takeda J, Okada M, Kobayashi J, Kato A, Hirota K, Taoka M, Matsumoto T, Komatsu K, Isobe T.
    • Journal Title

      Sci Rep.

      Volume: 3 Issue: 1 Pages: 2022-2022

    • DOI

      10.1038/srep02022

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Application of permanents of square matrices for DNA identification in multiple-fatality cases.2013

    • Author(s)
      Narahara M, Tamaki K, Yamada R
    • Journal Title

      BMC Genet.

      Volume: 14 Issue: 1 Pages: 72-72

    • DOI

      10.1186/1471-2156-14-72

    • NAID

      120005323880

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The unfolded protein response transducer ATF6 represents a novel transmembrane-type endoplasmic reticulum-associated degradation substrate requiring both mannose trimming and SEL1L protein.2013

    • Author(s)
      Horimoto S, Ninagawa S, Okada T, Koba H, Sugimoto T, Kamiya Y, Kato K, Takeda S, Mori K
    • Journal Title

      J Biol Chem.

      Volume: 288 Issue: 44 Pages: 31517-31527

    • DOI

      10.1074/jbc.m113.476010

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structure-specific endonucleases Xpf and Mus81 play overlapping but essential roles in DNA repair by homologous recombination.2013

    • Author(s)
      Kikuchi K, Narita T, Pham 〓, Iij〓 J, Hirota K, Islam SK, Mohiuddin M, Okawa K, Bori T, Fukagama T, Essers J, Kanser R, Whithy NC, Sugasawa K, Taniguchi T, Kitagawa K. Takeda S.
    • Journal Title

      Cancer Res.

      Volume: (in press) Issue: 14 Pages: 6917-6929

    • DOI

      10.1158/0008-5472.can-12-3154

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Interference in DNA replieation can cause mitotic chromosomal breakage unassociated with double-strand breaks2013

    • Author(s)
      Fujita Mari, Sasanuma H, Kimiyo N. Yamamoto Harada H, Kurosawa A, Adachi N, Omura M, Hiraoka M, Takeda S, Hirota K.
    • Journal Title

      PLos One

      Volume: 8 Issue: 4 Pages: 60043-60043

    • DOI

      10.1371/journal.pone.0060043

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Trapping of PARP1 and PARP2 by Clinical PARP Inhibitors.2012

    • Author(s)
      Murai J
    • Journal Title

      Cancer Res.

      Volume: 72 Issue: 21 Pages: 5588-5599

    • DOI

      10.1158/0008-5472.can-12-2753

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The SUMO protease SENP1 is required for cohesion maintenance and mitotic arrest following spendle poison treatment2012

    • Author(s)
      Saho Era
    • Journal Title

      Biochem. Biophys. Res. Comm.

      Volume: 426 Issue: 3 Pages: 310-316

    • DOI

      10.1016/j.bbrc.2012.08.066

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] TDP2 promotes repair of topoisomerase I-mediated DNA damage in the absence of TDP1.2012

    • Author(s)
      Zeng Z
    • Journal Title

      Nucleic Acids Res.

      Volume: 40 Pages: 8371-8380

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The helicase domain and C-terminus of human RecQL4 facilitate replication elongation on DNA templates damaged by ionizing radiation.2012

    • Author(s)
      Kohzaki M
    • Journal Title

      Carcinogenesis

      Volume: 33 Issue: 6 Pages: 1203-1210

    • DOI

      10.1093/carcin/bgs149

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Potentials and mechanisms of genotoxicity of six Pharmaceuticals frequently detected in freshwater environment2012

    • Author(s)
      Liu X, Takeda S, et al
    • Journal Title

      Toxicol Let

      Volume: 211 Issue: 1 Pages: 70-76

    • DOI

      10.1016/j.toxlet.2012.03.003

    • Related Report
      2012 Annual Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Tyrosyl-DNA phosphodi esterase 1 (TDP1) repairs DNA damages induced by topoisomerases I and II, and base alkylation in vertebrate cells2012

    • Author(s)
      Murai J, Takeda S, et al
    • Journal Title

      J Biol Chem

      Volume: 287 Issue: 16 Pages: 12848-12857

    • DOI

      10.1074/jbc.m111.333963

    • Related Report
      2012 Annual Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A central coupler for recombination initiation linking chromosome architecture to S phase checkpoint.2012

    • Author(s)
      三好知一郎
    • Journal Title

      Molecular Cell

      Volume: 14 Issue: 5 Pages: 722-733

    • DOI

      10.1016/j.molcel.2012.06.023

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Population model-based inter-diplotype similarity measure for accurate diplotype clustering.2012

    • Author(s)
      Onuki R
    • Journal Title

      J Comput Biol.

      Volume: 19 Issue: 1 Pages: 55-67

    • DOI

      10.1089/cmb.2010.0227

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genetic polymorphisms of the human PNPLA3 gene are strongly associated with severity of non-alcoholic fatty liver disease in Japanese.2012

    • Author(s)
      Kawaguchi T
    • Journal Title

      PLoS One

      Volume: 7 Issue: 6 Pages: e38322-e38322

    • DOI

      10.1371/journal.pone.0038322

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] hibition of Homologous Recombination by the PCNA-Interacting Protein PARI2012

    • Author(s)
      Moldovan GL, Takeda S, et al
    • Journal Title

      Mol Cell

      Volume: 45 Issue: 1 Pages: 75-86

    • DOI

      10.1016/j.molcel.2011.11.010

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A genome-wide association study identified AFF1 as a susceptibility locus for systemic lupus eyrthematosus in Japanese2012

    • Author(s)
      岡田随象
    • Journal Title

      PLoS Genetics

      Volume: (掲載確定) Issue: 1 Pages: 1002455-1002455

    • DOI

      10.1371/journal.pgen.1002455

    • NAID

      120005906866

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Meta-analysis identifies nine new loci associated with rheumatoid arthritis in the Japanese population2012

    • Author(s)
      Okada Y, Terao C, Ikari K, Kochi Y, Ohmura K, Suzuki A, Kawaguchi T, Stahl EA, Kurreeman FA, Nishida N, Ohmiya H, Myouzen K, Takahashi M, Sawada T, Nishioka Y, Yukioka M, Matsubara T, Wakitani S, Teshima R, Tohma S, Takasugi K, Shimada K, Murasawa A, Honjo S, Matsuo K, Tanaka H, Tajima K, Suzuki T, Iwamoto T, Kawamura Y, Tanii H, Okazaki Y, Sasaki T, Gregersen PK, Padyukov L, Worthington J, Siminovitch KA, Lathrop M, Taniguchi A, Takahashi A, Tokunaga K, Kubo M, Nakamura Y, Kamatani N, Mimori T, Plenge RM, Yamanaka H, Momohara S, Yamada R, Matsuda F, Yamamoto K.
    • Journal Title

      Nat Genet.

      Volume: (in press) Issue: 5 Pages: 511-6

    • DOI

      10.1038/ng.2231

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genotoxicity and endocrine disruption potentials of sediment near an oil spillt site : 2 years after the Hebei Spir oil spill2011

    • Author(s)
      Ji K, et al., Takeda S
    • Journal Title

      Environ Sci Technol

      Volume: 45 Issue: 11 Pages: 7481-7488

    • DOI

      10.1021/es104344e

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Purification of the Human SMN-GEMIN2 Complex and Assessment of Its Stimulation of RAD51-Mediated DNA Recombination Reactions2011

    • Author(s)
      Motoki Takaku, Takashi Tsujita, Naoki Horikoshi, Yoshimasa Takizawa, Yong, Qing, Kouji Hirota, Masae Ikura, Tsuyoshi Ikura, Shunichi Takeda, Hitoshi Kurumizaka
    • Journal Title

      Biochemistry

      Volume: 50 Issue: 32 Pages: 6797-6805

    • DOI

      10.1021/bi200828g

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The histone chaperone FACT maintains replication fork rates2011

    • Author(s)
      T.Abe, K.Sugimura, Y.Hosono, Y.Takami, M.Akita, A.Yoshimura, S.Tada, T.Nakayama, H.Murofushi, K.Okumura, S.Takeda, M.Horikoshi, M.Seki & T.Enomoto
    • Journal Title

      J.Biol.Chem.

      Volume: 286 Issue: 35 Pages: 30504-30512

    • DOI

      10.1074/jbc.m111.264721

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The epistatic relationship between BRCA2 and the other RAD51 mediators in homologous recombination2011

    • Author(s)
      Qing Y, et al
    • Journal Title

      PLoS Genet

      Volume: 7 Issue: 7 Pages: e1002148-e1002148

    • DOI

      10.1371/journal.pgen.1002148

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genotoxicity and endocrine disruption potentials of sediment near an oil spill site : 2 years after the Hebei Spirit oil spill2011

    • Author(s)
      Ji K, Takeda S, et al
    • Journal Title

      Environ Sci Technol

      Volume: 45 Issue: 17 Pages: 7481-7488

    • DOI

      10.1021/es200724x

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] SEL1L Is Required for Endoplasmic Reticulum-associated Degradation of Misfolded Luminal Proteins but not Transmembrane Proteins in Chicken DT40 Cell Line2011

    • Author(s)
      S. Ninagawa, T. Okada, S. Takeda and K. Mori
    • Journal Title

      Cell Structure and Function

      Volume: 36 Issue: 2 Pages: 187-195

    • DOI

      10.1247/csf.11018

    • NAID

      130004137570

    • ISSN
      0386-7196, 1347-3700
    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Regulation of the Fanconi anemia pathway by a SUMO-like delivery network2011

    • Author(s)
      Yang K, Takeda S, et al
    • Journal Title

      Genes Dev

      Volume: 25 Issue: 17 Pages: 1847-1858

    • DOI

      10.1101/gad.17020911

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Aurora A and Aurora B jointly coordinate chromosome segregation and anaphase microtubule dynamics2011

    • Author(s)
      Hegarat N, Takeda S, et al
    • Journal Title

      J Cell Biol

      Volume: 195 Issue: 7 Pages: 1103-1113

    • DOI

      10.1083/jcb.201105058

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The FOXE1 and NKX2-1 loci are associated with susceptibility to papillary thyroid carcinoma in the Japanese population2011

    • Author(s)
      Matsuse M, Takahashi M, Mitsutake N, Saenko V, Bychkov A, Suzuki K, Matsuo K, Yamashita S, et al
    • Journal Title

      J Med Genet

      Volume: 48(9) Issue: 9 Pages: 645-648

    • DOI

      10.1136/jmedgenet-2011-100063

    • NAID

      120006985044

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Association between the SERPING1 gene and age-related macular degeneration and polypoidal choroidal vasculopathy in Japanese2011

    • Author(s)
      Nakata I, Yamashiro K, Yamada R, Gotoh N, Nakanishi H, Hayashi H, Tsujikawa A, Otani A, Saito M, Iida T, Oishi A, Matsuo K, Tajima K, Matsuda F, Yoshimura N
    • Journal Title

      PLoS One

      Volume: 6 Issue: 4 Pages: e19108-e19108

    • DOI

      10.1371/journal.pone.0019108

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Myelin basic protein as a novel genetic risk factor in rheumatoid arthritis-a genome-wide study combined with immunological analyses2011

    • Author(s)
      Terao C., et at
    • Journal Title

      PLoS ONE

      Volume: 6 Issue: 6 Pages: e20457-e20457

    • DOI

      10.1371/journal.pone.0020457

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] Evidence for translesion synthesis by DNA polymerase in vivo2016

    • Author(s)
      Takeda S, Tsuda M, Hirota K, Sale J
    • Organizer
      abcam, Maintenance of Genome Stability 2016
    • Place of Presentation
      Panama City, Panama
    • Year and Date
      2016-03-07
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] SUMO化蛋白質を認識するRING型ユビキチンリガーゼRNF4による、スピンドルアセンブリチェックポイント(SAC)活性維持と染色体ロスの防止2016

    • Author(s)
      廣田 耕志、高木 季代、津田 雅貴、村井 純子、Keka Islam、 成田 岳雄、藤田 真梨、笹沼 博之、小林 純也、武田 俊一
    • Organizer
      SUMO研究会
    • Place of Presentation
      大阪
    • Year and Date
      2016-01-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] PAR結合蛋白質ALC1/CHDL1とXRCC1による単鎖切断修復2016

    • Author(s)
      廣田 耕志、安井 明、荻 朋男、原田 浩、中村 純、Yves Pommier、 武田 俊一
    • Organizer
      染色体ワークショップ
    • Place of Presentation
      宮城
    • Year and Date
      2016-01-12
    • Related Report
      2015 Annual Research Report
  • [Presentation] RNF4によるH2A.Zを介した染色体制御機構の解明2016

    • Author(s)
      髙木 季代、廣田 耕志
    • Organizer
      染色体ワークショップ
    • Place of Presentation
      宮城
    • Year and Date
      2016-01-12
    • Related Report
      2015 Annual Research Report
  • [Presentation] 分裂酵母fbp1におけるゲノム高次構造変化による遺伝子発現精密制御機構の解明2016

    • Author(s)
      浅田 隆大、廣田 耕志
    • Organizer
      染色体ワークショップ
    • Place of Presentation
      宮城
    • Year and Date
      2016-01-12
    • Related Report
      2015 Annual Research Report
  • [Presentation] 分裂酵母fbp1の転写抑制時のクロマチン再構築機構の解明2015

    • Author(s)
      梅田 未来、廣田 耕志
    • Organizer
      日本分子生物学会年会
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] 酸素の有無による電離放射線照射時のDNA損傷の種類の解析2015

    • Author(s)
      大岡 正人、清水 直登、高木 季代、武田 俊一、廣田 耕志
    • Organizer
      日本分子生物学会年会
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] 分裂酵母fbp1遺伝子の転写活性化におけるGcn5HATとクロマチンリモデリング因子Snf21およびSnf22の機能の解析2015

    • Author(s)
      足立 朗、廣田 耕志
    • Organizer
      日本分子生物学会年会
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] 分裂酵母fbp1におけるゲノム高次構造変化による遺伝子発現精密制御機構の解明2015

    • Author(s)
      浅田 隆大、廣田 耕志
    • Organizer
      日本分子生物学会年会
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] Capability of proofreading-exonucreas-deficient replicative DNA polymerase d to perform translesion DNA synthesis2015

    • Author(s)
      Hirota K, Tsuda M, Mohiudin, Tsurimoto T, Livneh Z, Iwai S, Sale J, Takeda S
    • Organizer
      日本分子生物学会年会
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 分裂酵母fbp1の転写抑制時のクロマチン再構築機構の解明2015

    • Author(s)
      梅田 未来、廣田 耕志
    • Organizer
      バイオコンファレンス2015
    • Place of Presentation
      東京
    • Year and Date
      2015-11-06
    • Related Report
      2015 Annual Research Report
  • [Presentation] DNA修復経路欠損細胞を用いたタバコに含まれる化学物質の遺伝毒性評価2015

    • Author(s)
      大岡 正人、高沢 浩則、武田 俊一、廣田 耕志
    • Organizer
      バイオコンファレンス2015
    • Place of Presentation
      東京
    • Year and Date
      2015-11-06
    • Related Report
      2015 Annual Research Report
  • [Presentation] Differential roles of Mre11 nuclease in DNA damage responses in the budding yeast and mammalian cells2015

    • Author(s)
      Hoa NN, Kobayashi J, Omura M, Hirakawa M, Yang SH, Paull TT, Komatsu K, Takeda S, Sasanuma H
    • Organizer
      16th Ataxia-Telangiectasia Workshop (ATW2015)
    • Place of Presentation
      Beijing, China
    • Year and Date
      2015-10-11
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The Role of Mre11 Nuclease Myth and Facts2015

    • Author(s)
      Takeda S
    • Organizer
      16th Ataxia-Telangiectasia Workshop (ATW2015)
    • Place of Presentation
      Beijing, China
    • Year and Date
      2015-10-11
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The Role of Mre11 in double-strand break repair Myth and Facts2015

    • Author(s)
      Takeda S
    • Organizer
      The 2nd IFOM - KU Joint SymposiumPerspectives in cancer biology: Genomic variations and host-tumor interactions
    • Place of Presentation
      京都
    • Year and Date
      2015-10-06
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] SUMO-Targeted Ubiquitin Ligase RNF4 required for genome maintenance regulates stability of the histone variant H2A.Z2015

    • Author(s)
      Hirota K
    • Organizer
      International symposium on chromatin structure, dynamics and function
    • Place of Presentation
      兵庫
    • Year and Date
      2015-08-23
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The regulation of Schizosaccharomyces Pombe fbp1 gene transcription through local higher order genome structure by transcription regulators2015

    • Author(s)
      Asada R, Hirota K
    • Organizer
      International Symposium Chromatin Structure, Dynamics and Function
    • Place of Presentation
      兵庫
    • Year and Date
      2015-08-23
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Collaboration of BRCA1, CtIP, and Dna2 is required to initiate double-strand break repair by homologous recombination2015

    • Author(s)
      Hoa NN, Kobayashi J, Omura M, Tsuda M, Hirota K, Hirakawa M, Yang SH, Paull TT, Komatsu K, Takeda S, Sasanuma H
    • Organizer
      FASEB Science Research Conferences, Genetic Recombination and Genome Rearrangements
    • Place of Presentation
      Steamboat Springs, Colorado, USA
    • Year and Date
      2015-07-19
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The regulation of Schizosaccharomyces pombe fbp1 gene transcription through local DNA looping structure by transcription regulators2015

    • Author(s)
      Asada R, Hirota K
    • Organizer
      Pombe 2015
    • Place of Presentation
      神戸
    • Year and Date
      2015-06-21
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 抗がん剤の作用機序を遺伝学的手法を使って調べる2015

    • Author(s)
      武田 俊一
    • Organizer
      天理よろづ相談所学術講演会
    • Place of Presentation
      奈良
    • Year and Date
      2015-06-19
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Differential roles of Mre11 nuclease in DNA damage responses in the budding yeast and mammalian cells2015

    • Author(s)
      Takeda S
    • Organizer
      CGI International Symposium
    • Place of Presentation
      Ulsan, Korea
    • Year and Date
      2015-05-27
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Genetic Analysis of Proteins Involved in The Initial Step of Double-strand Break Repair2015

    • Author(s)
      Takeda S
    • Organizer
      15th International Congress of Radiation Research
    • Place of Presentation
      京都
    • Year and Date
      2015-05-25
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Collaboration of BRCA1, CtIP, and Dna2 is required to initiate double-strand break repair by homologous recombination.2015

    • Author(s)
      Sasanuma H, Takeda S
    • Organizer
      Keystone Symposia, DNA Replication and Recombination
    • Place of Presentation
      Whistler, British Columbia, Canada
    • Year and Date
      2015-03-01 – 2015-03-06
    • Related Report
      2014 Annual Research Report
  • [Presentation] RNF4は染色体ロスを防止する2014

    • Author(s)
      高木季代、津田雅貴、村井純子、武田俊一、廣田耕志
    • Organizer
      染色体ワークショップ
    • Place of Presentation
      広島
    • Year and Date
      2014-12-15 – 2014-12-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] 分裂酵母fbp1におけるクロマチン構造・ゲノム高次構造変化による遺伝子発現制御機構の解明 染色体ワークショップ2014

    • Author(s)
      浅田隆大、竹俣直道、Charles Hoffman、太田邦史、廣田耕志
    • Organizer
      染色体ワークショップ
    • Place of Presentation
      広島
    • Year and Date
      2014-12-15 – 2014-12-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] 複製ポリメラーゼδPOLD3/p66サブユニットはポリメラーゼζ経路と独立に損傷乗り越えに寄与する2014

    • Author(s)
      廣田耕志、村井純子、Guillaume Guilbaud、津田雅貴、成田岳雄、西原佳那、笹沼博之、釣本俊樹、Julian Sale、武田俊一
    • Organizer
      染色体ワークショップ
    • Place of Presentation
      広島
    • Year and Date
      2014-12-15 – 2014-12-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] 損傷乗り越えniokeruPrimPolと損傷乗り越えポリメラーぜPolζ/ηの関係2014

    • Author(s)
      廣田耕志
    • Organizer
      染色体ワークショップ
    • Place of Presentation
      広島
    • Year and Date
      2014-12-15
    • Related Report
      2014 Annual Research Report
  • [Presentation] 損傷乗り越えPrimPolと損傷乗り越えポリメラーぜPolζ/ηの関係2014

    • Author(s)
      廣田耕志
    • Organizer
      分子生物学会
    • Place of Presentation
      横浜
    • Year and Date
      2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] SUMOターゲットユビキチンリガーゼ(STUbL)、RNF111は複製ストレストレランスに重要な役割を果たす2014

    • Author(s)
      川澄遼太郎、廣田耕志
    • Organizer
      分子生物学会
    • Place of Presentation
      横浜
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] Tup family corepressor とCCAATーbinding factorによるクロマチン構造変化の拮抗的な制御がmRNA転写開始点を決定する2014

    • Author(s)
      浅田隆大、竹俣直道、太田邦史、廣田耕志
    • Organizer
      分子生物学会
    • Place of Presentation
      横浜
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] SUMO化タンパク質を認識するRING型ユビキチンリガーゼRNF4による、スピンドルアセンブリチェックポイント(SAC)の活性維持と染色体ロスの防止2014

    • Author(s)
      廣田耕志、高木季代、津田雅貴、村井純子、Keka Islam、成田岳雄、藤田真梨、笹沼博之、小林純也、武田俊一
    • Organizer
      分子生物学会
    • Place of Presentation
      横浜
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] Collaboration of BRCA1, CtIP, and Dna2 is required to initiate double-strand break repair by homologous recombination.2014

    • Author(s)
      Sasanuma H, Takeda S
    • Organizer
      The 9th 3R Symposium Together with National Institute of Genetics “2014 International Symposium” “3R and beyond”
    • Place of Presentation
      御殿場
    • Year and Date
      2014-11-17 – 2014-11-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] EDEM1/2/3 are α1,2-mannosidases essential for endoplasmic reticulum-associated degradation of glycoproteins.2014

    • Author(s)
      Ninagawa S
    • Organizer
      SFG JSCR Joint Meeting
    • Place of Presentation
      Hawaii, USA
    • Year and Date
      2014-11-16 – 2014-11-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] Collaboration of BRCA1, CtIP, and Dna2 is required to initiate double-strand break repair by homologous recombination.2014

    • Author(s)
      武田俊一、笹沼博之
    • Organizer
      第73回日本癌学会学術総会
    • Place of Presentation
      横浜
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 分裂酵母fbp1におけるクロマチン構造変化を利用した遺伝子発現制御機構の解明2014

    • Author(s)
      浅田隆大、廣田耕志
    • Organizer
      酵母遺伝学フォーラム
    • Place of Presentation
      東京
    • Year and Date
      2014-09-01 – 2014-09-03
    • Related Report
      2014 Annual Research Report
  • [Presentation] The p66 subunit of DNA polymerase δ promotes translesion synthesis independently of DNA polymerase ζ.2014

    • Author(s)
      Hirota K, Sale J.E., Takeda S
    • Organizer
      ZING Conferences, DNA Polymerases: Biology, Diseases an Biomedical Applications
    • Place of Presentation
      Cambridge, UK
    • Year and Date
      2014-08-31 – 2014-09-04
    • Related Report
      2014 Annual Research Report
  • [Presentation] 小胞体で新生タンパク質に付加されたN型糖鎖からのマンノーストリミングはEDEM2によって開始される2014

    • Author(s)
      蜷川暁
    • Organizer
      第33回日本糖質学会
    • Place of Presentation
      名古屋
    • Year and Date
      2014-08-10 – 2014-08-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] A subunit of polymerase, p66 promotes translesion DNA synthesis independently of polymerase pathway.2014

    • Author(s)
      Hirota K, Yoshikiyo K, Murai J, Tsuda M, Takeda S
    • Organizer
      International Symposium on Xeroderma Pigmentosum and Related Diseases: Disorders of DNA Damage Response -Bench to Bedside-
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report
  • [Presentation] Collaboration between the CtIP and Dna2 nucleases is required to create 3' single-stranded ends at double-strand breaks in homologous recombination.2014

    • Author(s)
      Nguyen NH, Kobayashi J, Omura M, Tsuda M, Hirota K, Komatsu K, Takeda S, Sasanuma H
    • Organizer
      Fusion Conferences, Functional Polymeric Materials
    • Place of Presentation
      Cancun, Mexico
    • Related Report
      2013 Annual Research Report
  • [Presentation] Mutants deficient in DNA repair pathways provide high throughput assays for genotoxicity of chemicals and contribute to development of in silico methods for predicting genotoxicity of chemicals from their structure.2013

    • Author(s)
      Takeda S
    • Organizer
      FAOBMB Conference 2013
    • Place of Presentation
      Dakka, Bangladish
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Stereospecific trapping of PARP-DNA complexes by BMN 673 and comparison with olaparib and rucaparib.2013

    • Author(s)
      Murai J, Huang SN, Renaud A, Zhang Y, Ji J, Takeda S, Morris J, Teicher B, Doroshow JH, Pommier Y
    • Organizer
      第22回DNA複製・組換え・修復ワークショップ
    • Place of Presentation
      仙台
    • Related Report
      2013 Annual Research Report
  • [Presentation] The non-ubiquitylation and catalytic functioning of the RAD18 and RNF8 E3 ubiquitin ligases have substantial overlapping roles in SDB repaor and genome maintenance.2013

    • Author(s)
      Takeda S, Kobayashi S, Hirota K
    • Organizer
      The 5th EMBO Meeting 2013
    • Place of Presentation
      Amsterdam, Netherlands
    • Related Report
      2013 Annual Research Report
  • [Presentation] Collaboration between the CtIP and Dna2 nucleases is required to create 3’ single-stranded ends at double-strand breaks in homologous recombination.2013

    • Author(s)
      Takeda S, Nguyen NH, Sasanuma H
    • Organizer
      2013 FASEB Science Research Conferences, Genetic Recombination & Genome Rearrangements
    • Place of Presentation
      Steamboat Springs, Colorado, USA
    • Related Report
      2013 Annual Research Report
  • [Presentation] クロマチンリモデリング因子ALC1はPARP経路による酸化DNA損傷トレランスに必須の役割を果たす2013

    • Author(s)
      廣田耕志
    • Organizer
      第36回日本分子生物学会
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report
  • [Presentation] 染色体断裂はいつもDNA2重鎖切断が原因とは限らない2013

    • Author(s)
      Hirota K
    • Organizer
      第72回日本癌学会
    • Place of Presentation
      横浜
    • Related Report
      2013 Annual Research Report 2012 Annual Research Report
    • Invited
  • [Presentation] クロマチンリモデリング因子ALC1はPARP経路による酸化ダメージDNA損傷トレランスに必須の役割を果たす2013

    • Author(s)
      廣田耕志
    • Organizer
      遺伝研研究会
    • Place of Presentation
      静岡
    • Related Report
      2013 Annual Research Report
  • [Presentation] ニワトリBリンパ球DT40細胞を用いた遺伝毒物学手法による有害化学物質の検出法の開発と、その応用による抗がんシーズ化合物のスクリーニング2013

    • Author(s)
      廣田耕志
    • Organizer
      Biotech 2013
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
  • [Presentation] iPOP解析におけるデータ解析手法の統一化2013

    • Author(s)
      山田亮
    • Organizer
      日本人類遺伝学会
    • Place of Presentation
      仙台
    • Related Report
      2013 Annual Research Report
  • [Presentation] DNA二重鎖切断末端修飾におけるCtIPとDna2蛋白質の機能解析2013

    • Author(s)
      笹沼博之, Nguyen Ngoc Hoa, 小林純也, 小村将人, 津田雅貴, 廣田耕志, 小松賢志, 武田俊一
    • Organizer
      第36回日本分子生物学会
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report
  • [Presentation] Collaboration between the CtIP and Dna2 nucleases is required to create 3' single-stranded ends at double-strand breaks in homologous recombination.2013

    • Author(s)
      Sasanuma H, Nguyen NH, Kobayashi J, Omura M, Tsuda M, Hirota K, Komatsu K, Takeda S
    • Organizer
      RBC Symposium
    • Place of Presentation
      京都
    • Related Report
      2013 Annual Research Report
  • [Presentation] DNA二重鎖切断末端の削り込みにおけるCtIPとDna2による協調的な働き2013

    • Author(s)
      笹沼博之, Nguyen Ngoc Hoa, 小林純也, 小村将人, 津田雅貴, 廣田耕志, 小松賢志, 武田俊一
    • Organizer
      第22回DNA複製・組換え・修復ワークショップ
    • Place of Presentation
      仙台
    • Related Report
      2013 Annual Research Report
  • [Presentation] 遺伝学的手法を応用した、新規抗がん剤のスクリーニングとその作用機序の解析2013

    • Author(s)
      武田 俊一
    • Organizer
      金沢大学薬学シンポジウム2012 -DNA損傷応答を標的とした革新的がん創薬研究-
    • Place of Presentation
      金沢
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Genetic and Biochemical evidence for translesion DNA synthesis by replicative DNA polymerase delta2013

    • Author(s)
      Murai J
    • Organizer
      Keystone Symposia Conference, Genomic Instability and DNA Repair
    • Place of Presentation
      Banff, Canada
    • Related Report
      2012 Annual Research Report
  • [Presentation] 遺伝子破壊ニワトリBリンパ球を用いたクロロ酢酸のDNA障害とアポトーシス阻害の検討2013

    • Author(s)
      清水 宏泰
    • Organizer
      第83回日本衛生学会学術総会
    • Place of Presentation
      金沢
    • Related Report
      2012 Annual Research Report
  • [Presentation] Analysis of ERAD components SEL1L and EDEM1/2/3 using single, double and triple knockout cells of chicken DT402012

    • Author(s)
      Ninagawa S
    • Organizer
      FASEB Summer research conferences : From Unfolded Proteins in the Endoplasmic Reticulum to Disease
    • Place of Presentation
      Saxtons River, Vermont Academy, USA
    • Year and Date
      2012-06-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] The complex functional relationship between the RAD18 and RNF8 E3 ubiquitin lieases in DNA-damage responses2012

    • Author(s)
      Takeda S, Kobayashi S, Hirota K
    • Organizer
      The Sixth International Conference, SUMO, Ubiquitin, UBL Proteins : Implications for Human
    • Place of Presentation
      The University of Texas MD Anderson Cancer Center, Houston, USA
    • Year and Date
      2012-02-09
    • Related Report
      2011 Annual Research Report
  • [Presentation] Genetic models for DNA repair and cancer2012

    • Author(s)
      Takeda S
    • Organizer
      Tenth CMC Winter Symposium, Cellular and Molecular Medicine, Cancer, Stem Cells & Inflammation
    • Place of Presentation
      Christian Medical College, Vellore, India(招待講演)
    • Year and Date
      2012-01-06
    • Related Report
      2011 Annual Research Report
  • [Presentation] Rad51AP1, Rad54 and Rad54B play an overlapping role in strand exchange in homologous DNA recombination, and are essential for genome maintenance in higher eukaryotes2012

    • Author(s)
      Takeda S
    • Organizer
      EMBO Workshop, Recombination Mechanisms and Genome Instability
    • Place of Presentation
      Jerez de la Frontera, Spain
    • Related Report
      2012 Annual Research Report
  • [Presentation] Genetic and Biocheical evidence for translesion DNA synthesis by replicative DNA polymerase delta2012

    • Author(s)
      Takeda S
    • Organizer
      Gordon Research Conference, Mutagenesis
    • Place of Presentation
      Newport, USA
    • Related Report
      2012 Annual Research Report
  • [Presentation] Xpf and Mus81 are required to process homologous recombination intermediates2012

    • Author(s)
      Takeda S
    • Organizer
      EMBO Workshop, Structure-specific nucleases in DNA replication and repair
    • Place of Presentation
      Hyeres, France
    • Related Report
      2012 Annual Research Report
  • [Presentation] Analysis of homologous recombination using the chicken B cell line, DT402012

    • Author(s)
      Takeda S
    • Organizer
      IFOM-Kyoto University Joint Symposium, Mechanisms of cell transformation and metastasis
    • Place of Presentation
      Milan, Italy
    • Related Report
      2012 Annual Research Report
  • [Presentation] SUMO-targetted Ubiquitin Ligase RNF4 Guards Genome Stability by suppressing error-prone Homologous Recombination2012

    • Author(s)
      Hirota K
    • Organizer
      第35回日本分子生物学会
    • Place of Presentation
      博多
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Isotonic regression-based method for high throughput genotoxicity screenings2012

    • Author(s)
      Fujii Y
    • Organizer
      Congress of the European Societies of Toxicology
    • Place of Presentation
      Stockholm, Sweden
    • Related Report
      2012 Annual Research Report
  • [Presentation] 単調減少モデルによる遺伝毒性ハイスループットスクリーニングの解析2012

    • Author(s)
      藤井 庸祐
    • Organizer
      日本人類遺伝学会
    • Place of Presentation
      東京
    • Related Report
      2012 Annual Research Report
  • [Presentation] ERAD因子EDEMsは、小胞体におけるメジャーなマンノシダーゼである~ニワトリDT40細胞を用いたEDEM1/2/3トリプルKO細胞の解析~2012

    • Author(s)
      蜷川 暁
    • Organizer
      第12回日本蛋白質科学会年会
    • Place of Presentation
      名古屋
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] ER-associated degradation component EDEM promotes degradation of ER stress sensor ATF6 in vertebrate cells2012

    • Author(s)
      Horimoto S
    • Organizer
      FASEB Summer Research Conferences”Protein Folding in the Cell”
    • Place of Presentation
      Saxton's River, USA
    • Related Report
      2012 Annual Research Report
  • [Presentation] Mutants deficient in DNA repair pathways provide high throughput assays for genotoxicity of chemicals and contribute to development of in silico methods for predicting genotoxicity of chemicals from their structure2011

    • Author(s)
      Takeda S
    • Organizer
      International Scientific Conference, Regenerative Medicine and Healthy Aging
    • Place of Presentation
      Center for Life Sciences of Nazarbayev University, Astana, Kazakhstan(招待講演)
    • Year and Date
      2011-11-11
    • Related Report
      2011 Annual Research Report
  • [Presentation] 産業現場で使用される有害化学物質スクリーニングとしてのニワトリBリンパ球(DT40)によるバイオアッセイの確立2011

    • Author(s)
      清水宏泰、武田俊一, 他と河野公一
    • Organizer
      第51回近畿産業衛生学会
    • Place of Presentation
      奈良県文化会館(奈良県)
    • Year and Date
      2011-11-05
    • Related Report
      2011 Annual Research Report
  • [Presentation] Reverse Genetic Analysis of Protein Quality Control System in the ER2011

    • Author(s)
      Ninagawa S
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都国際会館(京都府)
    • Year and Date
      2011-09-23
    • Related Report
      2011 Annual Research Report
  • [Presentation] Reverse Genetic study of Structure-Specific-Endonucleases, FAN1, MUS81, and XPF in Homologous Recombination and Fanconi Anemia Repair Pathways2011

    • Author(s)
      Kikuchi K, et al., Takeda S
    • Organizer
      Responses to DNA damage : from molecular mechanism to human disease
    • Place of Presentation
      Egmond aan Zee, The Netherlands
    • Related Report
      2011 Annual Research Report
  • [Presentation] Complex functional relationship of the UBC13 E2 ubiquitin conjugating enzyme, and the RAD18 and RNF8 E3 ubiguitin ligases2011

    • Author(s)
      Takeda S, Kobayashi S
    • Organizer
      FASEB Summer Research Conferences, Genetic Recombination & Genome Rearrangements
    • Place of Presentation
      Steamboat Springs, Colorado, USA
    • Related Report
      2011 Annual Research Report
  • [Book] 医学のあゆみ、DNA損傷修復の概念提起からメカニズム解明へ2015

    • Author(s)
      廣田 耕志、武田 俊一
    • Total Pages
      5
    • Publisher
      医歯薬出版
    • Related Report
      2015 Annual Research Report
  • [Book] 実験医学増刊、酵母SRG1, mlonRNAによる遺伝子発現制御2015

    • Author(s)
      浅田 隆大、廣田 耕志
    • Total Pages
      2
    • Publisher
      羊土社
    • Related Report
      2015 Annual Research Report
  • [Book] 月刊血液内科 血液内科領域における抗腫瘍薬の作用機序・副作用に基づく使い分け シスプラチン耐性の分子機構2012

    • Author(s)
      笹沼 博之
    • Total Pages
      6
    • Publisher
      科学評論社
    • Related Report
      2012 Annual Research Report
  • [Remarks] DNAが切れていないのに発生する染色体断裂の発見-ヒトの被爆線量を測定する手法に異議あり-

    • URL

      http://www.kyoto-u.ac.jp/ja/news_data/h/h1/news6/2013/130404_1.htm

    • Related Report
      2013 Annual Research Report
  • [Remarks] 京都大学大学院 理学研究科 森研究室

    • URL

      http://www.upr.biophys.kyoto-u.ac.jp/research/

    • Related Report
      2013 Annual Research Report
  • [Remarks] DNAが切れていないのに発生する染色体断裂の発見-ヒトの被爆線量を測定する手法に異議あり-

    • URL

      http://www.kyoto-u.ac.jp/ja/news_data/h/h1/news6/2013/130404_1.htm

    • Related Report
      2012 Annual Research Report
  • [Remarks] 京都大学大学院 理学研究科 森研究室

    • URL

      http://www.upr.biophys.kyoto-u.ac.jp/research/

    • Related Report
      2012 Annual Research Report

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Published: 2011-06-18   Modified: 2022-02-07  

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