| Project/Area Number |
23227006
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| Research Category |
Grant-in-Aid for Scientific Research (S)
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional biochemistry
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| Research Institution | Waseda University (2013)
Kyoto Sangyo University (2011-2012) |
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Principal Investigator |
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| Project Period (FY) |
2011-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥106,210,000 (Direct Cost: ¥81,700,000、Indirect Cost: ¥24,510,000)
Fiscal Year 2013: ¥50,700,000 (Direct Cost: ¥39,000,000、Indirect Cost: ¥11,700,000)
Fiscal Year 2012: ¥50,310,000 (Direct Cost: ¥38,700,000、Indirect Cost: ¥11,610,000)
Fiscal Year 2011: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
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| Keywords | ATP / ATPsynthase / FoF1 / F1 / IF1 / ATP synthesis / Orf47 / ＡＴＰ合成 / ＡＴＰ合成酵素 / 細胞エネルギー / ミトコンドリア / 回転モーター / 生体エネルギー変換 / ATP合成 / 酵素の調節 |
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| Outline of Final Research Achievements |
We found the followings. Bacterial F1 generates at rotation angle 0, 40, 80°in one 120 cycle. Torque at 40°has been unnoticed before. Chroloplast ATP synthase of field spinach is activated at morning by reducing S-S bond of its gamma subunit and inactivated at night by oxidation. Human F1 rotates with three steps in a 120 cycle; 0→65°by ATP binding, 65→90°by Pi release, and 90→120°by ATP hydrolysis. A new factor Orf47 is required for assembly of human mitochondrial ATP synthase. Knockout mice that lack mitochondrial IF1, an inhibitor protein for ATP synthase, was produced. Surprisingly, they are healthy.
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| Assessment Rating |
Verification Result (Rating)
A-
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Assessment Rating |
Result (Rating)
A-: Progress in the research is steadily towards the initial goal. However, further efforts are necessary as a part of the research progress is delayed.
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