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Comprehensive elucidation and application of the function of enzymes involved in cleavage and synthesis of a carbon-nitrogen bond

Research Project

Project/Area Number 23228002
Research Category

Grant-in-Aid for Scientific Research (S)

Allocation TypeSingle-year Grants
Research Field Applied biochemistry
Research InstitutionUniversity of Tsukuba

Principal Investigator

KOBAYASHI Michihiko  筑波大学, 生命環境系, 教授 (70221976)

Co-Investigator(Kenkyū-buntansha) 熊野 匠人  筑波大学, 生命環境系, 助教 (70585025)
橋本 義輝  筑波大学, 生命環境系, 准教授 (00323254)
東端 啓貴  東洋大学, 生命科学部, 准教授 (20344864)
Project Period (FY) 2011-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥193,050,000 (Direct Cost: ¥148,500,000、Indirect Cost: ¥44,550,000)
Fiscal Year 2015: ¥35,880,000 (Direct Cost: ¥27,600,000、Indirect Cost: ¥8,280,000)
Fiscal Year 2014: ¥39,650,000 (Direct Cost: ¥30,500,000、Indirect Cost: ¥9,150,000)
Fiscal Year 2013: ¥39,520,000 (Direct Cost: ¥30,400,000、Indirect Cost: ¥9,120,000)
Fiscal Year 2012: ¥43,030,000 (Direct Cost: ¥33,100,000、Indirect Cost: ¥9,930,000)
Fiscal Year 2011: ¥34,970,000 (Direct Cost: ¥26,900,000、Indirect Cost: ¥8,070,000)
Keywords微生物 / 酵素 / 代謝 / 精製 / バイオテクノロジー
Outline of Final Research Achievements

Enzymes involved in the cleavage and synthesis of a carbon-nitrogen bond (except for the peptide bond) are very important in applied as well as academic fields. In this project, we tried to elucidate the function of enzymes involved in the cleavage and synthesis of a carbon-nitrogen bond. Moreover, we constructed fundamental techniques for the production of useful compounds using their catalytic activities.

Assessment Rating
Verification Result (Rating)

A-

Assessment Rating
Result (Rating)

A-: Progress in the research is steadily towards the initial goal. However, further efforts are necessary as a part of the research progress is delayed.

Report

(9 results)
  • 2017 Research Progress Assessment (Verification Result) ( PDF )
  • 2016 Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report   Abstract(Research Progress Assessment) ( PDF )   Research Progress Assessment (Result) ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (45 results)

All 2017 2016 2015 2014 2013 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Acknowledgement Compliant: 3 results,  Open Access: 1 results) Presentation (40 results) (of which Int'l Joint Research: 4 results,  Invited: 8 results) Remarks (1 results)

  • [Journal Article] Amide compound synthesis by adenylation domain of bacillibactin synthetase2017

    • Author(s)
      Abe, T., Hashimoto, Y., Sugimoto, S., Kobayashi, K., Kumano, T., & Kobayashi, M.
    • Journal Title

      J. Antibiot.,

      Volume: 70 Issue: 4 Pages: 435-442

    • DOI

      10.1038/ja.2016.117

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] New function of aldoxime dehydratase: Redox catalysis and the formation of an expected product2017

    • Author(s)
      Yamada, M., Hashimoto, Y., Kumano, T., Tsujimura, S. & Kobayashi, M.
    • Journal Title

      PLOS ONE

      Volume: 12 Issue: 4 Pages: e0175846-e0175846

    • DOI

      10.1371/journal.pone.0175846

    • NAID

      120007129139

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Peptide Bond Synthesis by a Mechanism Involving an Enzymatic Reaction and a Subsequent Chemical Reaction2016

    • Author(s)
      Abe, T., Hashimoto, Y., Zhuang, Y., Ge, Y., Kumano, T., & Kobayashi, M.
    • Journal Title

      J. Biol. Chem.,

      Volume: 291 Issue: 4 Pages: 1735-1750

    • DOI

      10.1074/jbc.m115.700989

    • NAID

      120007128491

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Crystal structure of aldoxime dehydratase and its catalytic mechanism involved in carbon-nitrogen triple-bond synthesis2013

    • Author(s)
      Nomura, J., Hashimoto, H., Ohta, T., Hashimoto, Y., Wada, K., Naruta, Y., Oinuma, K-I., & Kobayashi, M.
    • Journal Title

      Proc. Natl. Acad. Sci. USA

      Volume: 110 Issue: 8 Pages: 2810-2815

    • DOI

      10.1073/pnas.1200338110

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] 枯草菌細胞壁合成に関与する酵素による新規ペプチド合成反応における反応機構解析2016

    • Author(s)
      安部 智子、橋本 義輝、荘 葉、熊野 匠人、葛 寅、小林 達彦
    • Organizer
      2016年度日本農芸化学会大会
    • Place of Presentation
      札幌コンベンションセンター、北海道・札幌市
    • Related Report
      2015 Annual Research Report
  • [Presentation] Adenylate-forming superfamily酵素における新規ペプチド/アミド合成の反応機構解析2016

    • Author(s)
      橋本 義輝、安部 智子、熊野 匠人、荘 葉、葛 寅、小林 達彦
    • Organizer
      2016年度日本農芸化学会大会
    • Place of Presentation
      札幌コンベンションセンター、北海道・札幌市
    • Related Report
      2015 Annual Research Report
  • [Presentation] ニトリル前駆体アナログの微生物代謝2016

    • Author(s)
      石上 佳奈、橋本 義輝、熊野 匠人、小林 達彦
    • Organizer
      2016年度日本農芸化学会大会
    • Place of Presentation
      札幌コンベンションセンター、北海道・札幌市
    • Related Report
      2015 Annual Research Report
  • [Presentation] ニトリル合成酵素の多機能性2015

    • Author(s)
      山田 優駿、橋本 義輝、熊野 匠人、小林 達彦
    • Organizer
      2015年度日本農芸化学会大会
    • Place of Presentation
      岡山県岡山市、岡山大学
    • Year and Date
      2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] 微生物代謝および酵素の分子機構と機能開発2015

    • Author(s)
      小林 達彦
    • Organizer
      2015年度日本農芸化学会大会 日本農芸化学会賞受賞講演
    • Place of Presentation
      岡山県岡山市、岡山大学
    • Year and Date
      2015-03-26
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Microbial degradation of an analog of nitrile precursor2015

    • Author(s)
      Yoshiteru Hashimoto, Suguru Ohsuka and Michihiko Kobayashi
    • Organizer
      2015 International Symposium on New Frontiers in Microbiology and Biotechnology
    • Place of Presentation
      無錫、中国
    • Year and Date
      2015-01-12
    • Related Report
      2014 Annual Research Report
    • Int'l Joint Research
  • [Presentation] New synthetic formation of a carbon-nitrogen bond by unique enzyme function2015

    • Author(s)
      Michihiko Kobayashi
    • Organizer
      BIT’s 6th Annual Symposium of Enzyme & Biocatalysis 2015
    • Place of Presentation
      南京・中国
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] アルドキシム脱水酵素の多機能性2015

    • Author(s)
      山田 優駿、橋本 義輝、熊野 匠人、小林 達彦
    • Organizer
      BMB2015(第 38回日本分子生物学会年会、第 88回日本生化学会大会 合同大会)
    • Place of Presentation
      神戸ポートアイランド、兵庫県・神戸市
    • Related Report
      2015 Annual Research Report
  • [Presentation] ニトリル前駆体アナログ分解菌の培養条件検討2014

    • Author(s)
      大須賀 勝、橋本 義輝、熊野 匠人、小林 達彦
    • Organizer
      第87回日本生化学会大会
    • Place of Presentation
      京都府京都市、国立京都国際会館
    • Year and Date
      2014-10-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] 枯草菌細胞壁合成に関与する酵素の基質特異性解析2014

    • Author(s)
      橋本 義輝、荘 葉、安部 智子、葛 寅、熊野 匠人、小林 達彦
    • Organizer
      第87回日本生化学会大会
    • Place of Presentation
      京都府京都市、国立京都国際会館
    • Year and Date
      2014-10-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] Enzymatic dipeptide synthesis2014

    • Author(s)
      T.Abe,Y.Hashimoko,Y.Zhuang,Y.Ge,T.Kumano,M.Kobayashi
    • Organizer
      FEBS EMBO 2014 Conference
    • Place of Presentation
      Paris, France
    • Year and Date
      2014-09-09
    • Related Report
      2014 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Substrate specificity in peptide synthetic reaction2014

    • Author(s)
      Y.Hashimoto,T.Abe,Y.Zhuang,Y.Ge,T.Kumano,M.Kobayashi
    • Organizer
      FEBS EMBO 2014 Conference
    • Place of Presentation
      Paris, France
    • Year and Date
      2014-09-09
    • Related Report
      2014 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 枯草菌細胞壁合成に関与する酵素を用いたペプチド合成の基質特異性2014

    • Author(s)
      荘 葉、橋本 義輝、安部 智子、葛 寅、熊野 匠人、小林 達彦
    • Organizer
      2014年度日本農芸化学会大会
    • Place of Presentation
      東京、明治大学
    • Year and Date
      2014-03-28
    • Related Report
      2013 Annual Research Report
  • [Presentation] Unique reaction of carbon-nitrogen triple bond synthetic enzyme2014

    • Author(s)
      Masatoshi Yamada, Yoshiteru Hashimoto, Takuto Kumano and Michihiko Kobayashi
    • Organizer
      2014 International Symposium on New Frontiers in Microbiology and Biotechnology
    • Place of Presentation
      Wuxi, China
    • Year and Date
      2014-03-14
    • Related Report
      2013 Annual Research Report
  • [Presentation] Microbial nitrile-synthetic enzyme involved in C-N triple bond formation: Unique function and reaction2013

    • Author(s)
      Junpei Nomura,Hiroshi Hashimoto,Takehiro Ohta,Yoshiteru Hashimoto and Michihiko Kobayashi
    • Organizer
      BioMicroWorld 2013
    • Place of Presentation
      Madrid, Spain
    • Year and Date
      2013-10-03
    • Related Report
      2013 Annual Research Report
  • [Presentation] Unique heme-containing enzyme involved in formation of carbon-nitrogen triple bond: Expression, structural and mechanistic understanding and the potential for nitrile synthesis2013

    • Author(s)
      Junpei Nomura,Hiroshi Hashimoto,Takehiro Ohta,Yoshiteru Hashimoto, Michihiko Kobayashi
    • Organizer
      Enzyme Engineering XXII
    • Place of Presentation
      Toyama, Japan
    • Year and Date
      2013-09-26
    • Related Report
      2013 Annual Research Report
  • [Presentation] 枯草菌細胞壁合成に関与する酵素を利用したオリゴペプチド合成2013

    • Author(s)
      荘 葉、橋本 義輝、安部 智子、熊野 匠人、小林 達彦
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      横浜、パシフィコ横浜
    • Year and Date
      2013-09-13
    • Related Report
      2013 Annual Research Report
  • [Presentation] 炭素-窒素3重結合形成に関わる酵素の構造機能解析2013

    • Author(s)
      小林 達彦
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      横浜、パシフィコ横浜
    • Year and Date
      2013-09-13
    • Related Report
      2013 Annual Research Report
  • [Presentation] ニトリル前駆体アナログ分解菌のスクリーニング2013

    • Author(s)
      大須賀 勝、橋本 義輝、熊野 匠人、小林 達彦
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      横浜、パシフィコ横浜
    • Year and Date
      2013-09-13
    • Related Report
      2013 Annual Research Report
  • [Presentation] アルドキシムデヒドラターゼの立体構造と反応機構解析2013

    • Author(s)
      野村 純平、橋本 博、太田 雄大、橋本 義輝、和田 浩一、成田 吉徳、老沼 研一、山田 優駿、小林 達彦
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      横浜、パシフィコ横浜
    • Year and Date
      2013-09-11
    • Related Report
      2013 Annual Research Report
  • [Presentation] Unique enzyme reaction involved in the synthesis of a carbon-nitrogen bond2013

    • Author(s)
      Michihiko Kobayashi
    • Organizer
      2013 Annual Meeting of Society for Industrial Microbiology & Biotechnology (SIMB
    • Place of Presentation
      San Diego, USA
    • Year and Date
      2013-08-12
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Nitrile-synthetic enzyme involved in the formation of a carbon-nitrogen triple bond2013

    • Author(s)
      M.Kobayashi, J.Nomura and Y.Hashimoto
    • Organizer
      Experimental Biology 2013
    • Place of Presentation
      Boston, USA
    • Year and Date
      2013-04-23
    • Related Report
      2013 Annual Research Report
  • [Presentation] 微生物代謝の分子機構と物質生産への機能開発

    • Author(s)
      小林 達彦
    • Organizer
      BioJapan2012
    • Place of Presentation
      横浜、パシフィコ横浜
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Diverse metabolism of toxic isonitrile compounds and the degrading enzymes

    • Author(s)
      Michihiko Kobayashi
    • Organizer
      2012 Annual meeting, Society for Industrial Microbiology & Biotechnology
    • Place of Presentation
      Washington,DC, USA
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Unique biogenesis of carbon-nitrogen-cleaving enzyme harnessing a new mechanism

    • Author(s)
      Michihiko Kobayashi, Z. Zhou & Yoshiteru Hashimoto
    • Organizer
      2012 Annual meeting, American Society for Biochemistry and Molecular Biology
    • Place of Presentation
      San Diego, USA
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Aldoxime dehydratase: Crystal structure and catalytic mechanism involved in carbon-nitrogen triple bond synthesis

    • Author(s)
      Yoshiteru Hashimoto, Junpei Nomura, Hiroshi Hashimoto, Takehiro Ohta, Koichi Wada, Yoshinori Naruta, Ken-Ichi Oinuma & Michihiko Kobayashi
    • Organizer
      2013 International Symposium on New Frontiers in Microbiology and Biotechnology
    • Place of Presentation
      Wuxi, China
    • Related Report
      2012 Annual Research Report
  • [Presentation] Microbial Nitrile-synethetic Metabolism and the Enzyme

    • Author(s)
      Junpei Nomura, Ken-Ichi Oinuma, Yoshiteru Hashimoto & Michihiko Kobayashi
    • Organizer
      American Society for Microbiology 112th General Meeting
    • Place of Presentation
      San Francisco, USA
    • Related Report
      2012 Annual Research Report
  • [Presentation] Discovery of a novel enzyme involved in cleavage of a nitrogen-carbon triple bond

    • Author(s)
      Hiroyoshi Sato, Yoshiteru Hashimoto, & Michihiko Kobayashi
    • Organizer
      The 12th Japan-China-Korea Joint Symposium on Enzyme Engineering
    • Place of Presentation
      Kanazawa, Japan, Kanazawa Excel Hotel Tokyu
    • Related Report
      2012 Annual Research Report
  • [Presentation] 枯草菌細胞壁合成に関与する酵素を用いた新規ペプチド合成法

    • Author(s)
      荘 葉、橋本 義輝、安部 智子、葛 寅、熊野 匠人、小林 達彦
    • Organizer
      2013年度日本農芸化学会
    • Place of Presentation
      仙台、東北大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] 枯草菌細胞壁合成に関与する酵素の新規アミド結合触媒反応

    • Author(s)
      荘 葉、安部 智子、葛 寅、橋本 義輝、小林 達彦
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡、福岡国際会議場・マリンメッセ福岡
    • Related Report
      2012 Annual Research Report
  • [Presentation] Arthrobacter属放線菌由来新規イソニトリルヒドラターゼの部位特異的変異解析

    • Author(s)
      橋本 義輝、佐藤 太祐、小林 達彦
    • Organizer
      2012年度日本放線菌学会大会
    • Place of Presentation
      東京、府中の森芸術劇場
    • Related Report
      2012 Annual Research Report
  • [Presentation] 微生物と酵素の潜在機能の開拓: 探索と戦略

    • Author(s)
      小林 達彦
    • Organizer
      2012年度日本農芸化学会
    • Place of Presentation
      京都、京都女子大学
    • Related Report
      2011 Annual Research Report
  • [Presentation] 新規イソニトリルヒドラターゼの機能解析

    • Author(s)
      佐藤 太祐、橋本 義輝、小林 達彦
    • Organizer
      2012年度日本農芸化学会
    • Place of Presentation
      京都、京都女子大学
    • Related Report
      2011 Annual Research Report
  • [Presentation] ニトリル合成酵素の変異体構築および諸性質の解析

    • Author(s)
      野村 純平、橋本 義輝、小林 達彦
    • Organizer
      2012年度日本農芸化学会
    • Place of Presentation
      京都、京都女子大学
    • Related Report
      2011 Annual Research Report
  • [Presentation] シュードモナス属細菌由来ニトリル合成酵素の変異体解析

    • Author(s)
      野村 純平、橋本 義輝、小林 達彦
    • Organizer
      84回 生化学会大会
    • Place of Presentation
      京都、国立京都国際会館
    • Related Report
      2011 Annual Research Report
  • [Presentation] 新規イソニトリルヒドラターゼの諸性質と活性残基の同定

    • Author(s)
      佐藤 太祐、橋本 義輝、小林 達彦
    • Organizer
      84回 生化学会大会
    • Place of Presentation
      京都、国立京都国際会館
    • Related Report
      2011 Annual Research Report
  • [Presentation] Unique enzyme reactions involved in the formation of a carbon-nitrogen bond

    • Author(s)
      Michihiko Kobayashi
    • Organizer
      Enzyme Engineering XXI
    • Place of Presentation
      Colorado,USA
    • Related Report
      2011 Annual Research Report
    • Invited
  • [Presentation] New isonitrile hydratase from Arthrobacter: Characterization and identification of the active site

    • Author(s)
      Hiroyoshi Sato, Yoshiteru Hashimoto, and Michihiko Kobayashi
    • Organizer
      Internationar Union of Microbiological Societies 2011 Congress (IUMS 2011 Congress)
    • Place of Presentation
      札幌、札幌コンベンションセンター
    • Related Report
      2011 Annual Research Report
  • [Presentation] Microbial nitrile metabolism: Regulation and utilization

    • Author(s)
      Michihiko Kobayashi
    • Organizer
      Society for Industrial Microbiology 61st Annual Meeting (SIM)
    • Place of Presentation
      New Orleans,USA
    • Related Report
      2011 Annual Research Report
    • Invited
  • [Presentation] Microbial cleavage of nitrogen-carbon triple bond: Metabolism and enzyme

    • Author(s)
      Hiroyoshi Sato, Yoshiteru Hashimoto and Michihiko Kobayashi
    • Organizer
      American Society for Microbiology 111th General Meeting
    • Place of Presentation
      New Orleans, USA
    • Related Report
      2011 Annual Research Report
  • [Remarks] 研究室ホームページ

    • URL

      http://www.microbes.agbi.tsukuba.ac.jp/kobayashi/

    • Related Report
      2012 Annual Research Report

URL: 

Published: 2011-06-18   Modified: 2019-07-29  

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