|Budget Amount *help
¥107,380,000 (Direct Cost: ¥82,600,000、Indirect Cost: ¥24,780,000)
Fiscal Year 2015: ¥15,730,000 (Direct Cost: ¥12,100,000、Indirect Cost: ¥3,630,000)
Fiscal Year 2014: ¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2013: ¥20,280,000 (Direct Cost: ¥15,600,000、Indirect Cost: ¥4,680,000)
Fiscal Year 2012: ¥25,610,000 (Direct Cost: ¥19,700,000、Indirect Cost: ¥5,910,000)
Fiscal Year 2011: ¥28,730,000 (Direct Cost: ¥22,100,000、Indirect Cost: ¥6,630,000)
|Outline of Final Research Achievements
Natural killer T (NKT) cells are characterized by the expression of the single invariant Va14 receptor recognizing glycolipid antigens in the association with monomorphic CD1d molecule. However, their developmental pathway and molecular basis of their development remain unclear.
Here, we identified the novel developmental pathway for NKT cells directly generated from late DN stage precursors bypassing through DP stage and mainly migrated to the liver, but not to the fat, mesenteric lymphnode and lamina propria, where DP-derived NKT cells are migrated. We also found that 15 transcription factors, including Ring family proteins, were selectively expressed in DN-derived preNKT cells without surface Va14 receptor. Some of these TFs were shown to be responsible for NKT cell development in their knock-out mice. In addition, we succeeded to detect alteration of epigenetic modification responsible gene loci that regulate NKT cell development and function during NKT cell development.