Study of TRIC and MG23 channels

• Project/Area Number: 23240055
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurophysiology and muscle physiology
• Research Institution: Kyoto University
• Principal Investigator: TAKESHIMA Hiroshi 京都大学, 薬学研究科(研究院), 教授 (70212024)
• Co-Investigator(Kenkyū-buntansha): 山崎 大樹 京都大学, 学際融合教育研究推進センター, 特定助教 (40467428) ; 西 美幸 京都大学, 大学院薬学研究科, ・研究員 (60183894)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥49,400,000 (Direct Cost: ¥38,000,000、Indirect Cost: ¥11,400,000); Fiscal Year 2013: ¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000); Fiscal Year 2012: ¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000); Fiscal Year 2011: ¥20,800,000 (Direct Cost: ¥16,000,000、Indirect Cost: ¥4,800,000)
• Keywords: TRICチャネル / 小胞体Ca2+放出 / 血管平滑筋 / ミツグミン２３ / Ca2+スパーク / ミツグミン53 / TRIM50 / ミツグミン23 / 脂質二重膜再構成 / 単粒子解析 / リアノジン受容体 / 高血圧

Research Abstract

TRIC-A-knockout mice develop hypertension due to elevated myogenic tonus in resistance artery. By analyzing TRIC-A-knockout and overexpression mice, we found that TRIC-A density restricts Ca2+ spark frequency in vascular smooth muscle.
Mitsugumin 23 is an unknown membrane protein in the endo/sarcoplasmic reticulum. Our studies revealed that mitsugumin 23 forms an homo-maltimer with a bowl-like stereostructure and exhibits unstable cation channel activity.

Research Products

• [Journal Article] Enhanced dihydropyridine receptor calcium channel activity improves skeletal muscle strength (2013)
  • Author(s): Mosca, B., Bergamelli, L., Vukcevic, M, Lopez, R., Treves, S., Nishi, M., Takeshima, H., Paolini, C., Martini, M., Rispoli, G., Protasi, F. & Zorzato, F
  • Journal Title: Nat. Commun Volume: 4 Pages: 1541-1541
• [Journal Article] Facilitated hyperpolarization signaling in vascular smooth muscle overexpressing TRIC-A channels (2013)
  • Author(s): Tao, S., Yamazaki, D., Komazaki, S., Zhao, C., Iida, T., Kakizawa, S., Imaizumi, Y. & Takeshima, H
  • Journal Title: J. Biol. Chem Volume: 288 Pages: 15581-15589
• [Journal Article] TRIC channels supporting efficient Ca^<2+> release from intracellular stores (2013)
  • Author(s): Venturi, E., Sitsapesan, R., Yamazaki, D. & Takeshima, H
  • Journal Title: Pflugers Arch Volume: 465 Pages: 187-195
• [Journal Article] TRIC-B channels display labile gating : evidence from the TRIC-A knockout mouse model (2013)
  • Author(s): Venturi, E., Matyjaszkiewicz, A., Pitt, S J., Tsaneva-Atanasova, K., Nishi, M., Yamazaki, D., Takeshima, H. & Sitsapesan, R
  • Journal Title: Pflugers Arch Volume: 465 Pages: 1135-1148
• [Journal Article] Facilitated hyperpolarization signaling in vascular smooth muscle overexpressing TRIC-A channels. (2013)
  • Author(s): Tao, S., Yamazaki, D., Komazaki, S., Zhao, C., Iida, T., Kakizawa, S., Imaizumi, Y. & Takeshima, H.
  • Journal Title: J. Biol. Chem. Volume: 288 Issue: 22 Pages: 15581-15589
  • DOI: 10.1074/jbc.m112.435396
  • Peer Reviewed
• [Journal Article] TRIC-B channels display labile gating: evidence from the TRIC-A knockout mouse model. (2013)
  • Author(s): Venturi, E., Matyjaszkiewicz, A., Pitt, S. J., Tsaneva-Atanasova, K., Nishi, M., Yamazaki, D., Takeshima, H. & Sitsapesan, R.
  • Journal Title: Pflugers Arch. Volume: 465 Issue: 8 Pages: 1135-1148
  • DOI: 10.1007/s00424-013-1251-y
  • Peer Reviewed
• [Journal Article] Sarcalumenin plays a critical role in age-related cardiac dysfunction due to decreases in SERCA2a expression and activity (2012)
  • Author(s): Jiao, Q., Takeshima, H., Ishikawa, Y. & Minamisawa, S
  • Journal Title: Cell Calcium Volume: 51 Pages: 31-39
• [Journal Article] TRIM50 regulates vesicular trafficking for acid secretion in gastric parietal cells (2012)
  • Author(s): Nishi, M., Aoyama, F., Kisa, F., Zhu, H., Sun, M., Lin, P., Ohta, H., Yamamoto, S., Kakizawa, S., Sakai, H., Ma, J., Sawaguchi, A. & Takeshima, H
  • Journal Title: J. Biol. Chem Volume: 287 Pages: 33523-33532
• [Journal Article] Recombinant human MG53 protein modulates therapeutic cell membrane repair. (2012)
  • Author(s): Weisleder N, Takizawa N, Lin P, Wang X, Cao C, Zhang Y, Tan T, Ferrante C, Zhu H, Chen P-J, Yan R, Sterling M, Zhao X, Hwang M, Cai C, Cheng H, Takeshima H, Xiao R. & Ma J.
  • Journal Title: Sci. Transl. Med. Volume: 4 Issue: 139 Pages: 1-10
  • DOI: 10.1126/scitranslmed.3003921
  • Peer Reviewed
• [Journal Article] TRIM50 regulates vesicular trafficking for acid secretion in gastric parietal cells. (2012)
  • Author(s): Nishi, M., Aoyama, F., Kisa, F., Zhu, H., Sun, M., Lin, P., Ohta, H., Yamamoto, S., Kakizawa, S., Sakai, H., Ma, J., Sawaguchi, A. & Takeshima, H.
  • Journal Title: J. Biol. Chem. Volume: 287 Issue: 40 Pages: 33523-33532
  • DOI: 10.1074/jbc.m112.370551
  • Peer Reviewed
• [Journal Article] Mitsugumin 23 forms massive bowl-shaped assembly and cation-selective channel (2011)
  • Author(s): Venturi, E., Mio, K., Nishi, M., Ogura, T., Moriya, T., Okuda, K., Kakizawa, S., Sitapeasan, R., Chikara Sato, C. & Takeshima, H
  • Journal Title: Biochemistry Volume: 50 Pages: 2623-2632
• [Journal Article] TRIC-A channels in vascular smooth muscle contribute to blood pressure maintenance (2011)
  • Author(s): Yamazaki, D., Tabara, Y., Kita, S., Hanada, H., Komazaki, S., Naitou, D., Mishima, A., Nishi, M., Yamamura, H., Yamamoto, S., Kakizawa, S., Miyachi, H., Yamamoto, S., Miyata, T., Kawano, Y., Kamide, K., Ogihara, T., Hata, A., Umemura, S., Soma, M., Takahashi, N., Imaizumi, Y., Miki, T., Iwamoto, T. & Takeshima, H
  • Journal Title: Cell Metab Volume: 14 Pages: 231-241
  • NAID: 120003255678
• [Journal Article] TRIC-A channels in vascular smooth muscle contribute to blood pressure maintenance (2011)
  • Author(s): Yamazaki D, Tabara Y, Kita S, Hanada H, Komazaki S, Naitou D, Mishima A, Nishi M, Yamamura H, Yamamoto S, Kakizawa S, Miyachi H, Yamamoto S, Miyata T, Kawano Y, Kamide K, Ogihara T, Hata A, Umemura S, Soma M, Takahashi N, Imaizumi Y, Miki T, Iwamoto T, Ta
  • Journal Title: Cell Metab Volume: 14 Issue: 2 Pages: 231
  • DOI: 10.1016/j.cmet.2011.05.011
  • NAID: 120003255678
  • URL: https://pure.teikyo.jp/en/publications/ba8a77d6-2bdd-4164-afda-b1aeb465f18b http://hdl.handle.net/2433/143634
  • Peer Reviewed
• [Presentation] TRIC channels supporting physiological Ca^<2+> release (2012)
  • Author(s): Takeshima, H
  • Organizer: Gordon Research Conference; muscle E-C coupling
  • Place of Presentation: Les Diablerets, Switzerland
  • Year and Date: 2012-06-04
• [Presentation] TRIC channels are essential partners for Ca^<2+> release (2012)
  • Author(s): Takeshima, H
  • Organizer: European Calcium Channel Conference
  • Place of Presentation: Alpbach, Austria
  • Year and Date: 2012-05-19
• [Presentation] TRIC channel subtypes, willing partners with Ca^<2+> release channels in intracellular stores (2011)
  • Author(s): Takeshima, H
  • Organizer: Gordon Research Conference; Ca^<2+> signaling
  • Place of Presentation: Waterville Valley, USA
  • Year and Date: 2011-06-27
• [Presentation] TRIC channel subtypes, willing partners with Ca2+ release channels in intracellular stores (2011)
  • Author(s): Takeshima, H.
  • Organizer: Gordon Research Conference ; Ca2+ signaling
  • Place of Presentation: Watervilie Valley, USA(招待講演)
  • Year and Date: 2011-06-27
• [Presentation] TRIC channels and vascular tonus
  • Author(s): Takeshima, H.
  • Organizer: Gordon Research Conference; Ca2+ signaling
  • Place of Presentation: Tuscany Il Ciocco Resorts, Lucca, Italy
• [Presentation] TRIC channels supporting physiological Ca2+ release.
  • Author(s): Hiroshi Takeshima
  • Organizer: Gordon Research Conference; muscle E-C coupling
  • Place of Presentation: Les Diablerets, Switzerland
  • Invited
• [Book] TRIC チャネルと高血圧 (2013)
  • Author(s): 山崎大樹、陶晟辰、竹島浩
  • Publisher: Clinical Calcium
• [Remarks]
  • URL: http://www.pharm.kyoto-u.ac.jp/biochem/
• [Remarks] 所属研究室ホームページ
  • URL: http://www.pharm.kyoto-u.ac.jp/biochem/
• [Remarks] 研究室ホームページ
  • URL: http://www.pharm.kyoto-u.ac.jp/biochem/