Budget Amount *help |
¥49,010,000 (Direct Cost: ¥37,700,000、Indirect Cost: ¥11,310,000)
Fiscal Year 2014: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Fiscal Year 2013: ¥11,960,000 (Direct Cost: ¥9,200,000、Indirect Cost: ¥2,760,000)
Fiscal Year 2012: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
Fiscal Year 2011: ¥19,890,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥4,590,000)
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Outline of Final Research Achievements |
In order to explore new biomarkers which respond to exercise and stress, we performed proteomics using blood and urine samples before and after exhaustive exercise and identified the molecules using mass spectrometric analyses. Among the molecules which exhibited remarkable increases, we developed measurement system using ELISA. Also, we identified several microRNAs in the blood circulation in response to acute resistant exercise using microarray, and quantified expression levels using real-time PCR. Furthermore, we set up global analyses of translation dynamics using ribosome profiling for biomarker discovery.
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