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Development of novel biomarkers on exercise, stress and aging

Research Project

Project/Area Number 23240097
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied health science
Research InstitutionWaseda University

Principal Investigator

SUZUKI Katsuhiko  早稲田大学, スポーツ科学学術院, 教授 (80344597)

Co-Investigator(Kenkyū-buntansha) AKIMOTO Takayuki  東京大学, 大学院医学系研究科, 講師 (00323460)
Project Period (FY) 2011-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥49,010,000 (Direct Cost: ¥37,700,000、Indirect Cost: ¥11,310,000)
Fiscal Year 2014: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Fiscal Year 2013: ¥11,960,000 (Direct Cost: ¥9,200,000、Indirect Cost: ¥2,760,000)
Fiscal Year 2012: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
Fiscal Year 2011: ¥19,890,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥4,590,000)
Keywordsバイオマーカー / プロテオミクス / マイクロアレイ / リボソームプロファイリング / 運動 / ストレス / 筋損傷 / 炎症 / ホルモン / サイトカイン / マイクロRNA / 尿 / 老化 / エクソソーム / プロテオーム解析 / 酵素免疫測定法 / ウェスタンブロット / 活性酸素
Outline of Final Research Achievements

In order to explore new biomarkers which respond to exercise and stress, we performed proteomics using blood and urine samples before and after exhaustive exercise and identified the molecules using mass spectrometric analyses. Among the molecules which exhibited remarkable increases, we developed measurement system using ELISA. Also, we identified several microRNAs in the blood circulation in response to acute resistant exercise using microarray, and quantified expression levels using real-time PCR. Furthermore, we set up global analyses of translation dynamics using ribosome profiling for biomarker discovery.

Report

(5 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (35 results)

All 2015 2014 2013 2012 2011 Other

All Journal Article (16 results) (of which Peer Reviewed: 15 results,  Open Access: 3 results,  Acknowledgement Compliant: 3 results) Presentation (16 results) (of which Invited: 2 results) Book (1 results) Remarks (2 results)

  • [Journal Article] MicroRNA-23a has minimal effect on endurance exercise-induced adaptation of mouse skeletal muscle.2015

    • Author(s)
      S. Wada, Y. Kato, S. Sawada, K. Aizawa, J. H. Park, A. P. Russell, T. Ushida, T. Akimoto
    • Journal Title

      Pflügers Archiv

      Volume: 467 Issue: 2 Pages: 389-398

    • DOI

      10.1007/s00424-014-1517-z

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Changes of thioredoxin, oxidative stress markers, inflammation and muscle/renal damage following endurance exercise.2015

    • Author(s)
      K. Sugama, K. Suzuki, K. Yoshitani, K. Shiraishi, S. Miura, H. Yoshioka, Y. Mori, T. Kometani.
    • Journal Title

      Exer. Immunol. Rev.

      Volume: 21 Pages: 130-142

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Effect of aging and sex on circulating microRNAs in humans.2014

    • Author(s)
      S. Sawada, T. Akimoto, M. Takahashi, R. Sakurai, S. Shinkai, T. Ushida, Y. Fujiwara, K. Suzuki.
    • Journal Title

      Adv Aging Res

      Volume: 3 Issue: 02 Pages: 152-159

    • DOI

      10.4236/aar.2014.32023

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Evaluation of serum leaking enzymes and investigation into new biomarkers for exercise-induced muscle damage.2014

    • Author(s)
      Kanda K, Sugama K, Sakuma J, Kawakami Y. Suzuki K.
    • Journal Title

      Exer. Immunol. Rev. (EIR)

      Volume: 20 Pages: 39-54

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Exploring the importance of translational regulation in the inflammatory responses by a genome-wide approach.2014

    • Author(s)
      Sako H, Suzuki K.
    • Journal Title

      Exer. Immunol. Rev. (EIR)

      Volume: 20 Pages: 55-67

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of aging and sex on circulating microRNAs in humans.2014

    • Author(s)
      Sawada S, Akimoto T, Ushida T, Sakurai R, Shinkai S, Fujiwara Y, Suzuki K.
    • Journal Title

      Advances in Aging Research

      Volume: in press

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Exercise training attenuates adipose tissue fibrosis in diet-induced obese mice.2013

    • Author(s)
      Kawanishi K, Niihara H, Mizokami T, Yano H, Suzuki K.
    • Journal Title

      Biochem.Biophys.Res.Commun.(BBRC)

      Volume: 440/4 Pages: 774-779

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Exercise attenuates M1 macrophages and CD8+ T cells in adipose tissue of obese mice.2013

    • Author(s)
      Kawanishi N, Mizokami T, Yano H, Suzuki K.
    • Journal Title

      Med. Sci. Sports Exer. (MSSE)

      Volume: 45/9 Pages: 1684-1693

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The association between physical activity and sex-specific oxidative stress in older adults.2013

    • Author(s)
      Takahashi M, Miyashita M, Park JH, Kim HS, Nakamura Y, Sakamoto S, Suzuki K.
    • Journal Title

      J. Sports Sci. Med.

      Volume: 12/3 Pages: 571-578

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Profiling of circulating microRNAs after a bout of acute resistance exercise in humans.2013

    • Author(s)
      Sawada S, Kon M, Wada S, Ushida T, Suzuki K, Akimoto T.
    • Journal Title

      PLoS ONE

      Volume: 8/7

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] リボソームプロファイリングのバイオマーカー開発への応用2013

    • Author(s)
      佐古博皓
    • Journal Title

      日本補完代替医療学会誌

      Volume: 10/1 Pages: 1-7

    • Related Report
      2012 Annual Research Report
  • [Journal Article] Low-volume exercise training attenuates oxidative stress and neutrophils activation in older adults2013

    • Author(s)
      Masaki Takahashi, et al.
    • Journal Title

      European Journal of Applied Physiology

      Volume: 113 Issue: 5 Pages: 1117-1126

    • DOI

      10.1007/s00421-012-2531-5

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Urinary excretion of cytokines versus their plasma levels after endurance exercise.2013

    • Author(s)
      Sugama K
    • Journal Title

      Exerc. Immunol. Rev.

      Volume: 19 Pages: 64-84

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 運動ストレスに対する新規バイオマーカーとしての血中microRNA2012

    • Author(s)
      鈴木克彦
    • Journal Title

      日本補完代替医療学会誌

      Volume: 9(2) Pages: 69-74

    • NAID

      130001924033

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] IL-17, neutrophil activation and muscle damage following endurance exercise2012

    • Author(s)
      Sugama K, Suzuki K, Yoshitani K, Shiraishi K, Kometani T
    • Journal Title

      Exerc.Immunol.Rev.

      Volume: 18 Pages: 115-126

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Determination of chronic inflammatory states in cancer patients using assay of reactive oxygen species production by neutrophils2011

    • Author(s)
      Suzuki Y, Ohno S, Okuyama R, Aruga A, et al
    • Journal Title

      Antcancer Res.

      Volume: 32 Pages: 565-570

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] 一過性レジスタンス運動が血中マイクロRNAのプロファイルに及ぼす影響2012

    • Author(s)
      沢田秀司
    • Organizer
      第67回日本体力医学会学術総会
    • Place of Presentation
      岐阜
    • Related Report
      2012 Annual Research Report
  • [Presentation] 身体運動によるヘルスベネフィットのメカニズムを探る新たな実験モデル. シンポジウム10「身体運動によるヘルスベネフィットのメカニズムを探る」2012

    • Author(s)
      秋本崇之
    • Organizer
      第67回日本体力医学会学術総会
    • Place of Presentation
      岐阜
    • Related Report
      2012 Annual Research Report
  • [Presentation] 高齢者における12週間のウォーキング介入ならびにビタミンE摂取が血中の酸化ストレス指標に及ぼす影響2012

    • Author(s)
      高橋将記
    • Organizer
      第67回日本体力医学会学術総会
    • Place of Presentation
      東京都
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] The effects of sports drink composition on fluid intake and immunoendocrine responses to cycling in hot conditions2011

    • Author(s)
      K Suzuki, H Hashimoto, T Oh, T Ishijima, H Mitsuda, et al
    • Organizer
      11th International Symposium on Human & Sports Science
    • Place of Presentation
      Keimyung University, Daegu (KOREA)(招待講演)
    • Year and Date
      2011-12-17
    • Related Report
      2011 Annual Research Report
  • [Presentation] Salivary stress markers and inflammatory cytokines following exercise and the phase of the menstrual cycle2011

    • Author(s)
      H Hayashida, M Shimura, K Sugama, K Kanda, K Suzuki
    • Organizer
      11th International Symposium on Human & Sports Science
    • Place of Presentation
      Daegu (KOREA)
    • Year and Date
      2011-12-17
    • Related Report
      2011 Annual Research Report
  • [Presentation] Eccentric exercise-induced delayed-onset muscle soreness and changes in markers of muscle damage and inflammation2011

    • Author(s)
      K Kanda, K Sugama, H Hayashida K, K Suzuki., et al
    • Organizer
      11th International Symposium on Human & Sports Science
    • Place of Presentation
      Daegu (KOREA)
    • Year and Date
      2011-12-17
    • Related Report
      2011 Annual Research Report
  • [Presentation] The comparison of circulating cytokine responses depending on exercise intensity and their biological and pathological significance2011

    • Author(s)
      K Suzuki, K Tokizawa, M Okutsu, K Sugama, K Kanda, et al
    • Organizer
      10th Symposium of the International Society of Exercise and Immunology
    • Place of Presentation
      Oxford (UK)
    • Year and Date
      2011-07-11
    • Related Report
      2011 Annual Research Report
  • [Presentation] Changes in cytokines and leucocyte activation after low- and moderate- intensity exercise during menstruation2011

    • Author(s)
      H Hayashida, M Shimura, K Sugama, K Kanda, K Suzuki
    • Organizer
      10th Symposium of the International Society of Exercise and Immunology
    • Place of Presentation
      Oxford (UK)
    • Year and Date
      2011-07-11
    • Related Report
      2011 Annual Research Report
  • [Presentation] Aerobic exercise-induced changes of ankle brachial pressure index and menstrual cycle-dependent inflammation2011

    • Author(s)
      H Hayashida, M Shimura, K Suzuki
    • Organizer
      16th annual Congress of the European college of sport science
    • Place of Presentation
      Liverpool (UK)
    • Year and Date
      2011-07-08
    • Related Report
      2011 Annual Research Report
  • [Presentation] Assessment of neutrophil functional activity following prolonged endurance exercise.

    • Author(s)
      Suzuki K, Sugama K, Yoshitani Y, Shiraishi K, Kometani T, Miura S, Yoshioka H, Mori Y.
    • Organizer
      11th Symposium of the International Society of Exercise and Immunology
    • Place of Presentation
      Newcastle, Australia
    • Related Report
      2013 Annual Research Report
  • [Presentation] Genome-wide translational profiling of the inflammatory response of macrophage to LPS.

    • Author(s)
      Sako H, Suzuki K.
    • Organizer
      11th Symposium of the International Society of Exercise and Immunology
    • Place of Presentation
      Newcastle, Australia
    • Related Report
      2013 Annual Research Report
  • [Presentation] Genome-wide translational analysis reveals the impact of global translational dynamics in the early inflammatory response in macrophages.

    • Author(s)
      Sako H, Suzuki K.
    • Organizer
      EMBO workshop Complex Systems in Immunology
    • Place of Presentation
      Biopolis, Singapore
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Genome-wide translational analysis discovered the unique translational regulation independent of mRNA levels in the early inflammatory response of macrophages.

    • Author(s)
      Sako H, Suzuki K.
    • Organizer
      GTC Cytokines & Inflammation
    • Place of Presentation
      San Diego, U.S.A
    • Related Report
      2013 Annual Research Report
  • [Presentation] Changes of thioredoxin, oxidative stress markers and inflammatory responses after prolonged endurance exercise.

    • Author(s)
      Sugama K, Suzuki K, Yoshitani K, Shiraishi K, Kometani T
    • Organizer
      11th Symposium of the International Society of Exercise and Immunology
    • Place of Presentation
      Newcastle, Australia
    • Related Report
      2013 Annual Research Report
  • [Presentation] Changes of cytokines in mouse skeletal muscle and plasma by a single bout of endurance exercise.

    • Author(s)
      Lee M, Kawanishi N, Yada K, Hung Y, Mizokami T, Nihara H, Suzuki K.
    • Organizer
      11th Symposium of the International Society of Exercise and Immunology
    • Place of Presentation
      Newcastle, Australia
    • Related Report
      2013 Annual Research Report
  • [Presentation] 運動と免疫機能

    • Author(s)
      鈴木克彦
    • Organizer
      日本食品免疫学会第8回学術大会シンポジウム
    • Place of Presentation
      Velen, Germany
    • Related Report
      2012 Annual Research Report
  • [Book] Encyclopedia of Exercise Medicine in Health and Disease. Mooren FC, eds.2012

    • Author(s)
      Suzuki K. Cytokines.
    • Total Pages
      4
    • Publisher
      Springer
    • Related Report
      2012 Annual Research Report
  • [Remarks] 文部科学省 科研費

    • URL

      http://www.f.waseda.jp/katsu.suzu/mext.htm

    • Related Report
      2013 Annual Research Report
  • [Remarks] 研究者データベース公開

    • URL

      https://www.wnp7.waseda.jp/Rdb/app/ip/ipi0211.html?lang_kbn=0&kensaku_no=2134

    • Related Report
      2013 Annual Research Report

URL: 

Published: 2011-04-06   Modified: 2019-07-29  

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