Roles of newly discovered NBS1 domains in ubiquitin signals and rejoining of double-strand breaks after irradiation
Project/Area Number |
23241021
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Research Category |
Grant-in-Aid for Scientific Research (A)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Risk sciences of radiation/Chemicals
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Research Institution | Kyoto University |
Principal Investigator |
KOMATSU Kenshi 京都大学, 放射線生物研究センター, 教授 (80124577)
|
Co-Investigator(Kenkyū-buntansha) |
KOBAYASHI Junya 京都大学, 放射線生物研究センター, 准教授 (30301302)
KATO Akihiro 京都大学, 放射線生物研究センター, 研究員 (70423051)
|
Project Period (FY) |
2011-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥37,700,000 (Direct Cost: ¥29,000,000、Indirect Cost: ¥8,700,000)
Fiscal Year 2013: ¥12,480,000 (Direct Cost: ¥9,600,000、Indirect Cost: ¥2,880,000)
Fiscal Year 2012: ¥12,480,000 (Direct Cost: ¥9,600,000、Indirect Cost: ¥2,880,000)
Fiscal Year 2011: ¥12,740,000 (Direct Cost: ¥9,800,000、Indirect Cost: ¥2,940,000)
|
Keywords | 損傷乗越えDNA合成 / NBS1 / 損傷乗り越えDNA合成 / クロマチン・リモデリング / RNF20 / RAD18 / ATM / FACT / 相同組み換え修復 / 非相同末端再結合 / レポーター遺伝子 |
Outline of Final Research Achievements |
NBS1, a protein responsible for radiation sensitive disorder, has been to control both cell cycle checkpoint and homologous recombination repair by binding to either ATM or MRE11 at the C-terminus of NBS1. We hosed here other two binding regions at the C-terminus and their regulatory mechanisms. NBS1 ubiquitinates PCNA and then initiates Pol eta-mediated translesional DNA synthesis by binding to RAD18. It also binding to RNF20 to ubiquitinate histone H2B, which,in turn, recruits histone chaperon FACT and initiates chromatin-remodeling for rejoining of DNA double-strand break.
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Report
(4 results)
Research Products
(28 results)
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[Journal Article] Histone chaperone FACT regulates homologous recombination by chromatin remodeling through interaction with RNF20.2014
Author(s)
Oliveira DV, Kato A, Nakamura K, Ikura T, Okada M, Kobayashi J, Yanagihara H, Saito Y, Tauchi H, Komatsu K.
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Journal Title
J Cell Sci,
Volume: 127
Pages: 763-772
DOI
Related Report
Peer Reviewed
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[Journal Article] Mutations in the FHA-domain of ectopically expressed NBS1 lead to Radiosensitization and to no increase in somatic mutation rates via a partial suppression of homologous recombination2014
Author(s)
Maki Ohara, Yumi Funyu, Shunsuke Ebara, Yuki Sakamoto, Ryota Seki, Kenta Iijima, Akiko Ohishi, Junya Kobayashi, Kenshi Komatsu, Akira Tachibana, and Hiroshi Tauchi
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Journal Title
J. Radiat. Res
Volume: 55
Pages: in press
DOI
Related Report
Peer Reviewed
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