| Budget Amount *help |
¥46,150,000 (Direct Cost: ¥35,500,000、Indirect Cost: ¥10,650,000)
Fiscal Year 2014: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
Fiscal Year 2013: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
Fiscal Year 2012: ¥11,440,000 (Direct Cost: ¥8,800,000、Indirect Cost: ¥2,640,000)
Fiscal Year 2011: ¥15,730,000 (Direct Cost: ¥12,100,000、Indirect Cost: ¥3,630,000)
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| Outline of Final Research Achievements |
We have successfully established a novel cell fusion method based on field constriction created by microorifices. Using the method, fusion among ES-cells and tissue cells are performed, which resulted in bistable flat- and domelike- colony, which in 7 days showed typical colony shape of initialized cells. Another sample shown the initialization reporter from the colony. We have also shown the fusion of B cells and myeloma cell, which resulted in the hybridoma colony with the very high yield of 5%. These results show that our high-yield fusion method opens a way for cell initialization, as well as high yield production of mono-clonal antibodies.
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