Development of experimental techniques for the analysis of amplicomics

• Project/Area Number: 23241064
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Medical genome science
• Research Institution: Waseda University
• Principal Investigator: SEMBA Kentaro 早稲田大学, 理工学術院, 教授 (70206663)
• Co-Investigator(Kenkyū-buntansha): ITO Emi 福島医科大学, 医学部, 助教 (50540796) ; IMAI Junichi 福島医科大学, 医学部, 准教授 (70376739)
• Co-Investigator(Renkei-kenkyūsha): WATANABE Shinya 福島医科大学, 医学部, 教授 (70251444) ; WAGURI Satoshi 福島医科大学, 医学部, 教授 (30244908) ; ISOGAI Takao 福島医科大学, 医学部, 教授 (70456185) ; GOSHIMA Naoki 独立行政法人産業総合研究所, その他, 研究員 (70215482)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥49,270,000 (Direct Cost: ¥37,900,000、Indirect Cost: ¥11,370,000); Fiscal Year 2013: ¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000); Fiscal Year 2012: ¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000); Fiscal Year 2011: ¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
• Keywords: 疾患関連遺伝子 / 遺伝子増幅 / がん / ゲノム / 分子腫瘍学 / ゲノム発現 / 婦人科腫瘍学

Research Abstract

Cancer is a disease caused by mutations in genes. We carry two individual genes transmitted from father and mother, but in cancer cells, number of some genes is aberrantly increased by so-called "gene amplification". Accumulating evidence indicates that those genes, "oncogenes" can be used as target genes for diagnosis and therapy. Our research group has been focusing on breast cancer in which gene amplification is frequently observed and we have succeeded in developing several experimental procedures to discover oncogenes. For example, we established a protocol which enable us to efficiently introduce genes into normal cells to test transforming activity, namley whether or not they generate cancer cells. Finally, we found some genes which may serve as molecular diagnosis and targeted therapy for cancer. Furthermore, we performed functional analysis to know "why they make cancer".

Research Products

• [Journal Article] Gene amplification : mechanisms and involvement in cancer (2013)
  • Author(s): Matsui, A., Ihara, T., Suda, H., Mikami, H., and Semba, K
  • Journal Title: Biomolecular Concepts Volume: 4 Pages: 567-582
• [Journal Article] Gene amplification: mechanisms and involvement in cancer (2013)
  • Author(s): Semba, K et al.
  • Journal Title: Biomolecular Concepts Volume: 4 Pages: 567-582
  • DOI: 10.1515/bmc-2013-0026
  • Peer Reviewed
• [Journal Article] Expression screening of 17q12-21 amplicon reveals GRB7 as an ERBB2-dependent oncogene (2012)
  • Author(s): Saito, M., Kato, Y., Ito, E., Fujimoto, J., Ishikawa, K., Doi, A., Kumazawa, K., Matsui, A., Takebe, S., Ishida, T., Azuma, S., Mochizuki, H., Kawamura, Y., Yanagisawa, Y., Honma, R., Imai, J., Ohbayashi, H., Goshima, N., Semba, K., and Watanabe, S
  • Journal Title: FEBS letters Volume: 586 Pages: 1708-1714
• [Journal Article] Expression screening of 17q12-21 amplicon reveals GRB7 as an ERBB2-dependent oncogene. (2012)
  • Author(s): Saito M
  • Journal Title: FEBS Lett. Volume: 586 Pages: 1708-1714
  • Peer Reviewed
• [Journal Article] ErbB2/HER2 : Its Contribution to Basic Cancer Biology and the Development of Molecular Targeted Therapy, Breast Cancer - Carcinogenesis (2011)
  • Author(s): Yamamoto, T., Saito, M., Kumazawa, K., Doi, A., Matsui, A., Takebe, S., Amari, T., Oyama, M., and Semba, K
  • Journal Title: Cell Growth and Signalling Pathways, Prof. Mehmet Gunduz (Ed.) (InTech)
  • DOI: 10.5772/21965
• [Presentation] 発現制御可能な遺伝子改変マウス乳腺組織の作製 (2013)
  • Author(s): 伊原 辰哉
  • Organizer: 第36回日本分子生物学会年
  • Place of Presentation: 神戸国際展示場
  • Year and Date: 2013-12-04
• [Presentation] 幹細胞化を利用したマウス乳腺への簡便なトランスジーン技術の確立 (2013)
  • Author(s): 三上 紘史
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸国際展示場
  • Year and Date: 2013-12-04
• [Presentation] 初代培養乳腺上皮細胞を用いた3次元培養法の確立とその発癌機構解析への応用 (2013)
  • Author(s): 山本 瑞生
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸国際展示場
  • Year and Date: 2013-12-04
• [Presentation] 乳がん細胞株における遺伝子増幅領域を包括的に対象とした新規乳がん遺伝子のスクリーニング (2013)
  • Author(s): 石井 徹
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2013-10-05
• [Presentation] 三次元培養系を用いた新規乳癌関連遺伝子の同定 (2013)
  • Author(s): 須田 啓
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2013-10-03
• [Presentation] 上皮細胞株を用いたアンプリコン17q12-21 のスクリーニングによる新規がん遺伝子HNF1Bの同定と機能解析 (2013)
  • Author(s): 松井 貴香
  • Organizer: 第17回日本がん分子標的治療学会学術集会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2013-06-13
• [Presentation] 上皮細胞株を用いたアンプリコン17q12-21のスクリーニングによる新規がん遺伝子HNF1Bの同定と機能解析 (2013)
  • Author(s): 松井貴香
  • Organizer: 第17回日本がん分子標的治療学会学術集会
  • Place of Presentation: 国立京都国際会館
• [Presentation] 発現制御可能な遺伝子改変マウス乳腺組織の作製 (2013)
  • Author(s): 伊原 辰哉
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸国際展示場
• [Presentation] 浸潤に関わる新規乳癌遺伝子のスクリーニングのためのコラーゲンゲル三次元培養系の確立 (2012)
  • Author(s): 柴田 奈緒
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: ロイトン札幌
  • Year and Date: 2012-09-21
• [Presentation] 乳がんにおけるRARA(レチノイン酸受容体α)のEMT誘導因子としての機能 (2012)
  • Author(s): 土井 綾乃
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: さっぽろ芸文館
  • Year and Date: 2012-09-19
• [Presentation] E3 ユビキチンリガーゼをコードする新規形質転換遺伝子の同定 (2012)
  • Author(s): 加藤 悠希子
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌市教育文化会館
  • Year and Date: 2012-09-19
• [Presentation] Identification of a novel transforming gene encoding an E3 ubiquitin ligase-like protein (2012)
  • Author(s): Kato Y
  • Organizer: 日本癌学会学術総会
  • Place of Presentation: 札幌
• [Presentation] Oncogenic function of retinoic acid receptor alpha （RARA） as an epithelial-mesenchymal transition （EMT）-inducing factor (2012)
  • Author(s): Doi A
  • Organizer: 日本癌学会学術総会
  • Place of Presentation: 札幌
• [Presentation] Establishment of collagen gel 3D culture as a screening system for novel breast cancer oncogenes involved in invasion (2012)
  • Author(s): Shibata N
  • Organizer: 日本癌学会学術総会
  • Place of Presentation: 札幌
• [Presentation] GSC promotes tumorigenicity of ERBB2-expressing cells (2011)
  • Author(s): Kentaro Kumazawa
  • Organizer: Advances in breast cancer research : genetics, biology, and clinical applications
  • Place of Presentation: The Fairmont San Francisco
  • Year and Date: 2011-10-14
• [Presentation] RARA promotes the disruption of acinal structures induced by ERBB2 within the three-dimensional culture of MCF10A (2011)
  • Author(s): Ayano Doi
  • Organizer: Advances in breast cancer research : genetics, biology, and clinical applications
  • Place of Presentation: The Fairmont San Francisco
  • Year and Date: 2011-10-14
• [Presentation] Identification of a novel oncogene by systematic screening using NMuMG immortalized epithelial cells and a full-length cDNA expression library (2011)
  • Author(s): Atsuka Matsui
  • Organizer: Advances in breast cancer research : genetics, biology, and clinical applications
  • Place of Presentation: The Fairmont San Francisco
  • Year and Date: 2011-10-14
• [Presentation] Identification of a novel oncogene by systematic screening using NMuMG immortalized epithelial cells and a full-length cDNA expression library (2011)
  • Author(s): Atsuka Matsui
  • Organizer: Advances in breast cancer research : genetics, biology, and clinical applications
  • Place of Presentation: サンフランシスコ(アメリカ)
  • Year and Date: 2011-10-14
• [Presentation] GSC promotes tumorigenecity of ERBB2-expressing cells (2011)
  • Author(s): Kentaro Kumazawa
  • Organizer: Advances in breast cancer research : genetics, biology, and clinical applications
  • Place of Presentation: サンフランシスコ(アメリカ)
  • Year and Date: 2011-10-14
• [Presentation] RARA promotes the disruption of acinal structures induced by ERBB2 within the three-dimensional culture of MCF10A (2011)
  • Author(s): Ayano Doi
  • Organizer: Advances in breast cancer research : genetics, biology, and clinial applications
  • Place of Presentation: サンフランシスコ(アメリカ)
  • Year and Date: 2011-10-14
• [Presentation] ERBB2+サブタイプ乳癌の発がんにおけるEMT 誘導遺伝子の役割 (2011)
  • Author(s): 熊澤 建太郎
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2011-10-04
• [Presentation] Context-dependent oncogene in the ERBB2 amplicon : Identification of GRB7 as a bona fide supporter of ERBB2 (2011)
  • Author(s): 斉藤 諒
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2011-10-04
• [Presentation] MCF10A 三次元培養を利用した発癌の初期過程に関わる遺伝子スクリーニング系の確立 (2011)
  • Author(s): 土井 綾乃
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2011-10-04
• [Presentation] 上皮系細胞と全長cDNAを用いてのスクリーニングによる新規癌遺伝子の同定と機能の解析 (2011)
  • Author(s): 松井 貴香
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2011-10-04
• [Presentation] MCF10A三次元培養を利用した発癌の初期過程に関わる遺伝子スクリーニング系の確立 (2011)
  • Author(s): 土井綾乃
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場(名古屋)
  • Year and Date: 2011-10-04
• [Presentation] Context-dependent oncogene in the ERBB2 amplicon : Identification of GRB7 as a bona fide supporter of ERBB2 (2011)
  • Author(s): 斉藤諒
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場(名古屋)
  • Year and Date: 2011-10-04
• [Presentation] ERBB2+サブタイプ乳癌の発癌におけるEMT誘導遺伝子の役割 (2011)
  • Author(s): 熊澤建太郎
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場(名古屋)
  • Year and Date: 2011-10-04
• [Presentation] 上皮系細胞と全長cDNAを用いてのスクリーニングによる新規癌遺伝子の同定と機能の解析 (2011)
  • Author(s): 松井貴香
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場(名古屋)
  • Year and Date: 2011-10-03
• [Presentation] Identification of a context-dependent oncogene in ErbB2-positive cancer (2011)
  • Author(s): Makoto Saito
  • Organizer: Mechanisms and models of cancer
  • Place of Presentation: サンディエゴ(アメリカ)
  • Year and Date: 2011-08-14
• [Presentation] Identification of a Context-dependent Oncogene in ErbB2-positive Breast Cancer (2011)
  • Author(s): Makoto Saito
  • Organizer: Mechanisms & Models of Cancer
  • Place of Presentation: California・USA
  • Year and Date: 2011-08-10
• [Book] シグナル伝達キーワード事典 (2012)
  • Author(s): 山本 雅
  • Total Pages: 351
  • Publisher: 羊土社
• [Remarks]
  • URL: http://www.biomed.sci.waseda.ac.jp
• [Remarks] 早稲田大学 生命医科学科
  • URL: http://www.biomed.sci.waseda.ac.jp