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Development of experimental techniques for the analysis of amplicomics

Research Project

Project/Area Number 23241064
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Medical genome science
Research InstitutionWaseda University

Principal Investigator

SEMBA Kentaro  早稲田大学, 理工学術院, 教授 (70206663)

Co-Investigator(Kenkyū-buntansha) ITO Emi  福島医科大学, 医学部, 助教 (50540796)
IMAI Junichi  福島医科大学, 医学部, 准教授 (70376739)
Co-Investigator(Renkei-kenkyūsha) WATANABE Shinya  福島医科大学, 医学部, 教授 (70251444)
WAGURI Satoshi  福島医科大学, 医学部, 教授 (30244908)
ISOGAI Takao  福島医科大学, 医学部, 教授 (70456185)
GOSHIMA Naoki  独立行政法人産業総合研究所, その他, 研究員 (70215482)
Project Period (FY) 2011-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥49,270,000 (Direct Cost: ¥37,900,000、Indirect Cost: ¥11,370,000)
Fiscal Year 2013: ¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2012: ¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000)
Fiscal Year 2011: ¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Keywords疾患関連遺伝子 / 遺伝子増幅 / がん / ゲノム / 分子腫瘍学 / ゲノム発現 / 婦人科腫瘍学
Research Abstract

Cancer is a disease caused by mutations in genes. We carry two individual genes transmitted from father and mother, but in cancer cells, number of some genes is aberrantly increased by so-called "gene amplification". Accumulating evidence indicates that those genes, "oncogenes" can be used as target genes for diagnosis and therapy. Our research group has been focusing on breast cancer in which gene amplification is frequently observed and we have succeeded in developing several experimental procedures to discover oncogenes. For example, we established a protocol which enable us to efficiently introduce genes into normal cells to test transforming activity, namley whether or not they generate cancer cells. Finally, we found some genes which may serve as molecular diagnosis and targeted therapy for cancer. Furthermore, we performed functional analysis to know "why they make cancer".

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report

Research Products

(38 results)

All 2013 2012 2011 Other

All Journal Article (5 results) (of which Peer Reviewed: 2 results) Presentation (30 results) Book (1 results) Remarks (2 results)

  • [Journal Article] Gene amplification : mechanisms and involvement in cancer2013

    • Author(s)
      Matsui, A., Ihara, T., Suda, H., Mikami, H., and Semba, K
    • Journal Title

      Biomolecular Concepts

      Volume: 4 Pages: 567-582

    • Related Report
      2013 Final Research Report
  • [Journal Article] Gene amplification: mechanisms and involvement in cancer2013

    • Author(s)
      Semba, K et al.
    • Journal Title

      Biomolecular Concepts

      Volume: 4 Pages: 567-582

    • DOI

      10.1515/bmc-2013-0026

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression screening of 17q12-21 amplicon reveals GRB7 as an ERBB2-dependent oncogene2012

    • Author(s)
      Saito, M., Kato, Y., Ito, E., Fujimoto, J., Ishikawa, K., Doi, A., Kumazawa, K., Matsui, A., Takebe, S., Ishida, T., Azuma, S., Mochizuki, H., Kawamura, Y., Yanagisawa, Y., Honma, R., Imai, J., Ohbayashi, H., Goshima, N., Semba, K., and Watanabe, S
    • Journal Title

      FEBS letters

      Volume: 586 Pages: 1708-1714

    • Related Report
      2013 Final Research Report
  • [Journal Article] Expression screening of 17q12-21 amplicon reveals GRB7 as an ERBB2-dependent oncogene.2012

    • Author(s)
      Saito M
    • Journal Title

      FEBS Lett.

      Volume: 586 Pages: 1708-1714

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ErbB2/HER2 : Its Contribution to Basic Cancer Biology and the Development of Molecular Targeted Therapy, Breast Cancer - Carcinogenesis2011

    • Author(s)
      Yamamoto, T., Saito, M., Kumazawa, K., Doi, A., Matsui, A., Takebe, S., Amari, T., Oyama, M., and Semba, K
    • Journal Title

      Cell Growth and Signalling Pathways, Prof. Mehmet Gunduz (Ed.) (InTech)

    • DOI

      10.5772/21965

    • Related Report
      2013 Final Research Report
  • [Presentation] 発現制御可能な遺伝子改変マウス乳腺組織の作製2013

    • Author(s)
      伊原 辰哉
    • Organizer
      第36回日本分子生物学会年
    • Place of Presentation
      神戸国際展示場
    • Year and Date
      2013-12-04
    • Related Report
      2013 Final Research Report
  • [Presentation] 幹細胞化を利用したマウス乳腺への簡便なトランスジーン技術の確立2013

    • Author(s)
      三上 紘史
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸国際展示場
    • Year and Date
      2013-12-04
    • Related Report
      2013 Final Research Report
  • [Presentation] 初代培養乳腺上皮細胞を用いた3次元培養法の確立とその発癌機構解析への応用2013

    • Author(s)
      山本 瑞生
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸国際展示場
    • Year and Date
      2013-12-04
    • Related Report
      2013 Final Research Report
  • [Presentation] 乳がん細胞株における遺伝子増幅領域を包括的に対象とした新規乳がん遺伝子のスクリーニング2013

    • Author(s)
      石井 徹
    • Organizer
      第72回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2013-10-05
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] 三次元培養系を用いた新規乳癌関連遺伝子の同定2013

    • Author(s)
      須田 啓
    • Organizer
      第72回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2013-10-03
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] 上皮細胞株を用いたアンプリコン17q12-21 のスクリーニングによる新規がん遺伝子HNF1Bの同定と機能解析2013

    • Author(s)
      松井 貴香
    • Organizer
      第17回日本がん分子標的治療学会学術集会
    • Place of Presentation
      国立京都国際会館
    • Year and Date
      2013-06-13
    • Related Report
      2013 Final Research Report
  • [Presentation] 上皮細胞株を用いたアンプリコン17q12-21のスクリーニングによる新規がん遺伝子HNF1Bの同定と機能解析2013

    • Author(s)
      松井貴香
    • Organizer
      第17回日本がん分子標的治療学会学術集会
    • Place of Presentation
      国立京都国際会館
    • Related Report
      2013 Annual Research Report
  • [Presentation] 発現制御可能な遺伝子改変マウス乳腺組織の作製2013

    • Author(s)
      伊原 辰哉
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸国際展示場
    • Related Report
      2013 Annual Research Report
  • [Presentation] 浸潤に関わる新規乳癌遺伝子のスクリーニングのためのコラーゲンゲル三次元培養系の確立2012

    • Author(s)
      柴田 奈緒
    • Organizer
      第71回日本癌学会学術総会
    • Place of Presentation
      ロイトン札幌
    • Year and Date
      2012-09-21
    • Related Report
      2013 Final Research Report
  • [Presentation] 乳がんにおけるRARA(レチノイン酸受容体α)のEMT誘導因子としての機能2012

    • Author(s)
      土井 綾乃
    • Organizer
      第71回日本癌学会学術総会
    • Place of Presentation
      さっぽろ芸文館
    • Year and Date
      2012-09-19
    • Related Report
      2013 Final Research Report
  • [Presentation] E3 ユビキチンリガーゼをコードする新規形質転換遺伝子の同定2012

    • Author(s)
      加藤 悠希子
    • Organizer
      第71回日本癌学会学術総会
    • Place of Presentation
      札幌市教育文化会館
    • Year and Date
      2012-09-19
    • Related Report
      2013 Final Research Report
  • [Presentation] Identification of a novel transforming gene encoding an E3 ubiquitin ligase-like protein2012

    • Author(s)
      Kato Y
    • Organizer
      日本癌学会学術総会
    • Place of Presentation
      札幌
    • Related Report
      2012 Annual Research Report
  • [Presentation] Oncogenic function of retinoic acid receptor alpha (RARA) as an epithelial-mesenchymal transition (EMT)-inducing factor2012

    • Author(s)
      Doi A
    • Organizer
      日本癌学会学術総会
    • Place of Presentation
      札幌
    • Related Report
      2012 Annual Research Report
  • [Presentation] Establishment of collagen gel 3D culture as a screening system for novel breast cancer oncogenes involved in invasion2012

    • Author(s)
      Shibata N
    • Organizer
      日本癌学会学術総会
    • Place of Presentation
      札幌
    • Related Report
      2012 Annual Research Report
  • [Presentation] GSC promotes tumorigenicity of ERBB2-expressing cells2011

    • Author(s)
      Kentaro Kumazawa
    • Organizer
      Advances in breast cancer research : genetics, biology, and clinical applications
    • Place of Presentation
      The Fairmont San Francisco
    • Year and Date
      2011-10-14
    • Related Report
      2013 Final Research Report
  • [Presentation] RARA promotes the disruption of acinal structures induced by ERBB2 within the three-dimensional culture of MCF10A2011

    • Author(s)
      Ayano Doi
    • Organizer
      Advances in breast cancer research : genetics, biology, and clinical applications
    • Place of Presentation
      The Fairmont San Francisco
    • Year and Date
      2011-10-14
    • Related Report
      2013 Final Research Report
  • [Presentation] Identification of a novel oncogene by systematic screening using NMuMG immortalized epithelial cells and a full-length cDNA expression library2011

    • Author(s)
      Atsuka Matsui
    • Organizer
      Advances in breast cancer research : genetics, biology, and clinical applications
    • Place of Presentation
      The Fairmont San Francisco
    • Year and Date
      2011-10-14
    • Related Report
      2013 Final Research Report
  • [Presentation] Identification of a novel oncogene by systematic screening using NMuMG immortalized epithelial cells and a full-length cDNA expression library2011

    • Author(s)
      Atsuka Matsui
    • Organizer
      Advances in breast cancer research : genetics, biology, and clinical applications
    • Place of Presentation
      サンフランシスコ(アメリカ)
    • Year and Date
      2011-10-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] GSC promotes tumorigenecity of ERBB2-expressing cells2011

    • Author(s)
      Kentaro Kumazawa
    • Organizer
      Advances in breast cancer research : genetics, biology, and clinical applications
    • Place of Presentation
      サンフランシスコ(アメリカ)
    • Year and Date
      2011-10-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] RARA promotes the disruption of acinal structures induced by ERBB2 within the three-dimensional culture of MCF10A2011

    • Author(s)
      Ayano Doi
    • Organizer
      Advances in breast cancer research : genetics, biology, and clinial applications
    • Place of Presentation
      サンフランシスコ(アメリカ)
    • Year and Date
      2011-10-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] ERBB2+サブタイプ乳癌の発がんにおけるEMT 誘導遺伝子の役割2011

    • Author(s)
      熊澤 建太郎
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2011-10-04
    • Related Report
      2013 Final Research Report
  • [Presentation] Context-dependent oncogene in the ERBB2 amplicon : Identification of GRB7 as a bona fide supporter of ERBB22011

    • Author(s)
      斉藤 諒
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2011-10-04
    • Related Report
      2013 Final Research Report
  • [Presentation] MCF10A 三次元培養を利用した発癌の初期過程に関わる遺伝子スクリーニング系の確立2011

    • Author(s)
      土井 綾乃
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2011-10-04
    • Related Report
      2013 Final Research Report
  • [Presentation] 上皮系細胞と全長cDNAを用いてのスクリーニングによる新規癌遺伝子の同定と機能の解析2011

    • Author(s)
      松井 貴香
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2011-10-04
    • Related Report
      2013 Final Research Report
  • [Presentation] MCF10A三次元培養を利用した発癌の初期過程に関わる遺伝子スクリーニング系の確立2011

    • Author(s)
      土井綾乃
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場(名古屋)
    • Year and Date
      2011-10-04
    • Related Report
      2011 Annual Research Report
  • [Presentation] Context-dependent oncogene in the ERBB2 amplicon : Identification of GRB7 as a bona fide supporter of ERBB22011

    • Author(s)
      斉藤諒
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場(名古屋)
    • Year and Date
      2011-10-04
    • Related Report
      2011 Annual Research Report
  • [Presentation] ERBB2+サブタイプ乳癌の発癌におけるEMT誘導遺伝子の役割2011

    • Author(s)
      熊澤建太郎
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場(名古屋)
    • Year and Date
      2011-10-04
    • Related Report
      2011 Annual Research Report
  • [Presentation] 上皮系細胞と全長cDNAを用いてのスクリーニングによる新規癌遺伝子の同定と機能の解析2011

    • Author(s)
      松井貴香
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場(名古屋)
    • Year and Date
      2011-10-03
    • Related Report
      2011 Annual Research Report
  • [Presentation] Identification of a context-dependent oncogene in ErbB2-positive cancer2011

    • Author(s)
      Makoto Saito
    • Organizer
      Mechanisms and models of cancer
    • Place of Presentation
      サンディエゴ(アメリカ)
    • Year and Date
      2011-08-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] Identification of a Context-dependent Oncogene in ErbB2-positive Breast Cancer2011

    • Author(s)
      Makoto Saito
    • Organizer
      Mechanisms & Models of Cancer
    • Place of Presentation
      California・USA
    • Year and Date
      2011-08-10
    • Related Report
      2013 Final Research Report
  • [Book] シグナル伝達キーワード事典2012

    • Author(s)
      山本 雅
    • Total Pages
      351
    • Publisher
      羊土社
    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.biomed.sci.waseda.ac.jp

    • Related Report
      2013 Final Research Report
  • [Remarks] 早稲田大学 生命医科学科

    • URL

      http://www.biomed.sci.waseda.ac.jp

    • Related Report
      2012 Annual Research Report

URL: 

Published: 2011-04-05   Modified: 2019-07-29  

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