|Budget Amount *help
¥48,620,000 (Direct Cost: ¥37,400,000、Indirect Cost: ¥11,220,000)
Fiscal Year 2014: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
Fiscal Year 2013: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
Fiscal Year 2012: ¥14,820,000 (Direct Cost: ¥11,400,000、Indirect Cost: ¥3,420,000)
Fiscal Year 2011: ¥11,960,000 (Direct Cost: ¥9,200,000、Indirect Cost: ¥2,760,000)
|Outline of Final Research Achievements
First, we developed a method for classifying functional RNA combined graph theoretic approach with the software SHARAKU to calculate the mapping shape similarity for processing patterns. The proposed method was applied to the sequence data obtained by the next-generation sequencing for the tumor samples taken in the mouse carcinogenicity experiment, and comprehensive analysis of small derived RNA processed specifically at each stage in multistage carcinogenesis was carried out. Second, we developed a prediction program for protein-RNA interactions. We succeeded in developing a technique for contact prediction between residues-bases. Third, it was carried out verification experiments using transcriptome analysis of carcinogenicity for Meis1 gene of conditional knockout mouse.