| Project/Area Number |
23241073
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Chemical biology
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| Research Institution | Osaka University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
TAKEI Fumie 大阪大学, 産業科学研究所, 助教 (30252711)
HAGIHARA Masaki 弘前大学, 理工学研究科, 准教授 (40403000)
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| Research Collaborator |
DOHNO Chikara 大阪大学, 産業科学研究所, 准教授 (60420395)
MURATA Asako 大阪大学, 産業科学研究所, 助教 (50557121)
FUKUZUMI Takeo 大阪大学, 産業科学研究所, 特任助教 (00592768)
AIKAWA Haruo 大阪大学, 産業科学研究所, 特任助教 (70547322)
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| Project Period (FY) |
2011-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥49,270,000 (Direct Cost: ¥37,900,000、Indirect Cost: ¥11,370,000)
Fiscal Year 2013: ¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2012: ¥15,730,000 (Direct Cost: ¥12,100,000、Indirect Cost: ¥3,630,000)
Fiscal Year 2011: ¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
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| Keywords | synthesis / 8-substituted purine / chemical library / riboswitch / reengineering / ８位置換プリン誘導体 / in vivo セレクション / リボスイッチ / doped library / ８位置換プリン化合物 / ライブラリー / 8位置換プリン / インビトロセレクション |
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| Outline of Final Research Achievements |
In the projet "Synthesis of 8-substituted purine chemical library and ribolswitch-reengineering", we have focused our attention on the synthesis of compound library of 8-substituted purim derivatives, and the application of compounds for the regulation of artificial riboswitches. The synthesis of 8-substituted purine derivatives were successfully done by a coupling of 8-bromo-adenine and 8-bromo-adenosine with the commercially available borate esters under Suzuki-Miyaura coupling conditions, providing us a chemical library of 8-substituted purines. With these compounds in hand, we looked for the RNA functioning as riboswiches by in vitro selection using doped library of adenine riboswitch. We have changed the selection conditions, the desired RNA functioning as riboswitch could not be selected. The lack of diversity of RNA sequence was one of reason for these results.
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