Methanogenesis under high pressure conditions mimicking subsurface environments
| Project/Area Number |
23244109
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Geochemistry/Astrochemistry
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
KAMAGATA YOICHI 独立行政法人産業技術総合研究所, 生物プロセス研究部門, 研究部門長 (70356814)
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| Co-Investigator(Kenkyū-buntansha) |
SAKATA Susumu 産業技術総合研究所, 地圏資源環境部門研究, グループ長 (70357101)
吉岡 秀佳 産業技術総合研究所, 地圏資源環境部門, 主任研究員 (30415765)
皆川 秀紀 産業技術総合研究所, メタンハイドレート研究センター, 研究チーム長 (70202348)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥48,750,000 (Direct Cost: ¥37,500,000、Indirect Cost: ¥11,250,000)
Fiscal Year 2013: ¥14,170,000 (Direct Cost: ¥10,900,000、Indirect Cost: ¥3,270,000)
Fiscal Year 2012: ¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000)
Fiscal Year 2011: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
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| Keywords | メタン生成菌 / メタンハイドレート / 天然ガス / 高圧培養 / 微生物間共生 / 地下圏微生物 / 安定同位体比 / 水素 / メタン生成古細菌 / 高温微生物 / 高温性微生物 / 微生物共生 / 根源有機物 / 共生微生物 / 安定同位対比 / 酢酸酸化 |
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| Research Abstract |
We investigated whether biological methanogensis occurs under high pressure conditions (mimicking subsurface environments). We used pure cultures of hydrogenotrophic and aceticlastic methanogens, and an acetate oxidizing hydrogen producing syntroph as well as environmental subsurface samples taken from an oil field. We obtained the following results: 1) pure cultures of hydrogenotrophic and aceticlastic methanogens, and syntrophic coculture were successfully cultivated under high pressure conditions (up to 15MPa). Note that the syntrophic coculture grew and produced methane under such high pressure conditions. 2) Methoxylated benzoates were used as model kerogen derivatives to enrich communities that were capable of degrading them to produce methane. 3) In the acetate-utilizing methane-producing community, CO2 concentration is the crucial factor driving either aceticlastic methanogenesis or syntrophic acetate oxidation and subsequent methanogenesis from H2 and CO2.
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Report
(4 results)
Research Products
(21 results)
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[Journal Article] Methanothermobacter tenebrarum sp. nov., a hydrogenotrophic thermophilic methanogen isolated from gas-associated formation water of a natural gas field in Japan2013
Author(s)
Nakamura, K., A. Takahashi, C. Mori, H. Tamaki, H. Mochimaru, K. Nakamura, K. Takamizawa, and, Y. Kamagata
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Journal Title
International Journal of Systematic and Evolutionary Microbiology.
Volume: 63
Issue: Pt_2
Pages: 715-722
DOI
Related Report
Peer Reviewed
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