Determination of Inter-molecular Structures of Fibers and Polymers using Ultra-High Field Solid State NMR with Ultra-High Speed NMR Probe
| Project/Area Number |
23245045
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Polymer/Textile materials
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
ASAKURA TETSUO 東京農工大学, 工学(系)研究科(研究院), 教授 (30139208)
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| Co-Investigator(Kenkyū-buntansha) |
SHIMIZU Tadashi 独立行政法人物質, 中核機能部門強磁場ステーション, ステーション長 (00354366)
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| Co-Investigator(Renkei-kenkyūsha) |
NAKAZAWA Yasumoto 東京農工大学, 大学院工学研究院, 准教授 (20456255)
SUZUKI Yu 東京農工大学, 大学院工学研究院, 助教 (90600263)
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| Project Period (FY) |
2011-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥50,440,000 (Direct Cost: ¥38,800,000、Indirect Cost: ¥11,640,000)
Fiscal Year 2013: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
Fiscal Year 2012: ¥14,430,000 (Direct Cost: ¥11,100,000、Indirect Cost: ¥3,330,000)
Fiscal Year 2011: ¥26,390,000 (Direct Cost: ¥20,300,000、Indirect Cost: ¥6,090,000)
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| Keywords | 1H固体NMR構造解析 / 超高速固体NMRマイクロプローブ / 超高磁場NMR / 家蚕絹の繊維化前後の構造 / クモ絹の構造 / 水素結合 / 1H NMR 化学シフト / 絹の繊維化機構 / 高分子構造・物性 / 固体NMR / 絹 |
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| Outline of Final Research Achievements |
The analytical method for the structure determination of several fibers and polymer materials was proposed on the basis of newly developed 1H solid state NMR method. The method consists of the combination of microprobe with ultra-high speed and ultra-high field NMR magnet together with the theoretical chemical shift calculation. The 1H NMR peak assignment and 1H chemical shift data were obtained together with the inter-molecular atomic distance information using this analytical method. The structure of Bombyx mori silk fibroin before spinning was determined precisely and details of the hydrogen bonding formation which contributes to the structural stabilization were clarified. The structure of the silk fibroin after spinning was also determined precisely. Then the fiber formation mechanism was discussed on the basis of these two structures. Similarly, this 1H solid state NMR method was also applied to determination of the structure of the crystalline fraction in spider silk.
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Report
(4 results)
Research Products
(204 results)
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[Journal Article] Intermolecular Packing in B. mori Silk Fibroin: Multinuclear NMR Study of the Model Peptide (Ala-Gly)15 Defines a Heterogeneous Antiparallel Antipolar Mode of Assembly in the Silk II Form2015
Author(s)
Asakura, T., Ohata, T., Kametani S., Okushita, K., Yazawa, K., Nishiyama, Y., Nishimura, K., Aoki, A., Suzuki, F., Kaji, H., Anne S. Ulrich, and Mike P. Williamson
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Journal Title
Macromolecules
Volume: 48
Issue: 1
Pages: 28-36
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Silk Fibroin2015
Author(s)
Suzuki, Y., Asakura, T.
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Journal Title
Encyclopedia of Polymer Nanomaterials, Springer
Volume: 印刷中
Pages: 1-7
DOI
ISBN
9783642361999
Related Report
Peer Reviewed
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[Journal Article] Recombinant silk fibroin incorporated celladhesive sequences produced by transgenic silkworm as a possible candidate for use in vascular graft2014
Author(s)
Asakura, T., Isozaki, M., Saotome, T., Tatematsu, K., Sezutsu, H., Kuwabara, N., Nakazawa, Y.
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Journal Title
Journal of Materials Chemistry B
Volume: 2
Issue: 42
Pages: 7375-7383
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Silk fibroin-based scaffolds for bone regeneration2013
Author(s)
Kuboyama N, Kiba H, Arai K, Uchida R, Tanimoto Y, Bhawal UK, Abiko Y, Miyamoto S, Knight D, Asakura T, Nishiyama N.
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Journal Title
J Biomed Mater Res B Appl Biomater
Volume: Vol.101
Issue: 2
Pages: 295-302
DOI
Related Report
Peer Reviewed
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[Journal Article] Synthesis and Characterization of Water-Soluble Silk Peptides and Recombinant Silk Protein Containing Polyalanine, the Integrin Binding Site, and Two Glutamic Acids at Each Terminal Site as a Possible Candidate for Use in Bone Repair Materials2013
Author(s)
Asakura, T., Suzuki, Y., Nagano, A., Knight, D.P., Kamiya, M., Demura, M.
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Journal Title
Biomacromolecules
Volume: 14
Issue: 10
Pages: 3731-3741
DOI
Related Report
Peer Reviewed
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