| Budget Amount *help |
¥50,180,000 (Direct Cost: ¥38,600,000、Indirect Cost: ¥11,580,000)
Fiscal Year 2013: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000)
Fiscal Year 2012: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
Fiscal Year 2011: ¥29,120,000 (Direct Cost: ¥22,400,000、Indirect Cost: ¥6,720,000)
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| Outline of Final Research Achievements |
Tissue engineering is basically composed of the three technologies which are concerning cells, their culture and scaffold. This research is concerning the fabrication of tissue engineering scaffold with laser sintering, one of so-called 3D printer technology. In this research, short wave length of 1.1 microns was selected to reduce the laser spot diameter, while typical commerciall systems employ 11microns, and a prototype scaffold was successfully developed out of typical scaffold material of Polycaprolactone. Fabrication technique that reduces powder bed temperature was developed. Material of porogen powder was switched from sodium chloride to less heat conductive and alkaline soluble plastic. Owing two these developments, precision of scaffold was roughly doubled. Hydrophilicity treatment of PCL scaffold with alkaline solution was established to improve cell adhesion. Resultantly, stability of cell culture of HepG2 cells was improved.
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