Higher Speed Biosensing Techniques based on Nano-surgery System

• Project/Area Number: 23246044
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Intelligent mechanics/Mechanical systems
• Research Institution: Meijo University (2013); Nagoya University (2011-2012)
• Principal Investigator: FUKUDA Toshio 名城大学, 理工学部, 教授 (70156785)
• Co-Investigator(Kenkyū-buntansha): NAKAJIMA Masahiro 名古屋大学, 工学研究科, 助教 (80377837) ; 小嶋 勝 名古屋大学, 大学院・工学研究科, 特任助教 (00533647)
• Project Period (FY): 2011-05-31 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥49,660,000 (Direct Cost: ¥38,200,000、Indirect Cost: ¥11,460,000); Fiscal Year 2013: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000); Fiscal Year 2012: ¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000); Fiscal Year 2011: ¥22,880,000 (Direct Cost: ¥17,600,000、Indirect Cost: ¥5,280,000)
• Keywords: ナノサージェリ / バイオセンシング / マイクロ・ナノデバイス / モデル生物 / マイクロ流体デバイス / ナノデバイス / ナノマニピュレーション / ハイブリッド顕微鏡 / ナノツール / 電子顕微鏡 / ナノ加工

Research Abstract

Currently, the bio-sensing technologies have been received much attention for smart, safe, and high-sensitive analysis of environmental materials using living organism directly with some effects on ecosystem. In this research, we applied our nanomanipulation technique to realize single cell level local analysis using various nano-tools. We proposed a bio-indicator device for heavy metal inside some solution by controlling a micro-fluidic chip to use a model organism.

Research Products

• [Journal Article] A microfluidic device with multi valves system to enable simultaneous several exposure tests on Caenorhabditis elegans (2014)
  • Author(s): Jaehoon Jung, Masahiro Nakajima, Masaru Takeuchi, Qiang Huang, Toshio Fukuda
  • Journal Title: Journal of Micromechanics and Microengineering Volume: Vol.24, No.3 Pages: 35012-35012
  • Peer Reviewed
• [Journal Article] 53. A microfluidic device with multi valves system to enable simultaneous several exposure tests on Caenorhabditis elegans (2014)
  • Author(s): Jaehoon JUNG, Masahiro NAKAJIMA, Masaru TAKEUCHI, Qiang HUANG, and Toshio FUKUDA
  • Journal Title: Journal of Micromechanics and Microengineering Volume: 24 Issue: 3 Pages: 35012-35012
  • DOI: 10.1088/0960-1317/24/3/035012
  • Peer Reviewed
• [Journal Article] Microfluidic device for the continuous culture and analysis of Caenorhabditis elegans in a toxic aqueous environment (2013)
  • Author(s): Jaehoon Jung, Masahiro Nakajima, Hirotaka Tajima, Toshio Fukuda
  • Journal Title: Journal of Micromechatronics and Microengineering Volume: Vol.23 Pages: 085008-8
  • Peer Reviewed
• [Journal Article] Single cell stiffness measurement at various humidity conditions by nanomanipulation of a nano-needle (2013)
  • Author(s): Yajing Shen, Masahiro Nakajima, Zhan Yang, Hirotaka Tajima, Zoran Najdovski, Michio Homma, Toshio Fukuda
  • Journal Title: Nanotechnology Volume: Vol.24, Number 14 Pages: 145703-145703
  • Peer Reviewed
• [Journal Article] A microfluidic device for the continuous culture and analysis of Caenorhabditis elegans in a toxic aqueous environment (2013)
  • Author(s): Jaehoon JUNG, Masahiro NAKAJIMA, Hirotaka TAJIMA, Qiang HUANG, and Toshio FUKUDA
  • Journal Title: Journal of Micromechanics and Microengineering Volume: 1 Issue: 8 Pages: 85008-85008
  • DOI: 10.1088/0960-1317/23/8/085008
  • Peer Reviewed
• [Journal Article] Instantaneous and Quantitative Single Cells Viability Determination Using Dual Nanoprobe Inside ESEM (2012)
  • Author(s): Mohd Ridzuan Ahmad, Masahiro Nakajima, Masaru Kojima, Seiji Kojima, Michio Homma, Toshio Fukuda
  • Journal Title: IEEE Transactions on Nanotechnology Volume: Vol.11, No.2 Pages: 298-306
  • Peer Reviewed
• [Journal Article] Nanofork for Single Cells Adhesion Measurement via ESEM-Nanomanipulator System (2012)
  • Author(s): Mohd Ridzuan Ahmad, Masahiro Nakajima, Masaru Kojima, Seiji Kojima, Michio Homma, Toshio Fukuda
  • Journal Title: IEEE Transactions on Nanobioscience Volume: Vol.11, No.1 Pages: 70-78
  • Peer Reviewed
• [Journal Article] Microchip device for measurement of body volume of C. elegans as bioindicator application (2012)
  • Author(s): Jaehoon Jung, Masahiro Nakajima, Masaru Kojima, Katsutoshi Ooe, Toshio Fukuda
  • Journal Title: Journal of Micro - Nano Mechatronics Volume: Vol.7 Pages: 3-11
  • Peer Reviewed
• [Journal Article] Evaluation of the single yeast cell's adhesion to ITO substrates with various surface energies via ESEM nanorobotic manipulation system (2011)
  • Author(s): Yajing Shen, Mohd Ridzuan Ahmad, Masahiro Nakajima, Seiji Kojima, Michio Homma, Toshio Fukuda
  • Journal Title: IEEE Transactions on Nanobioscience Volume: Vol.10, No.4 Pages: 217-224
  • Peer Reviewed
• [Journal Article] Study of the time effect on the strength of cell-cell adhesion force by a novel nano-picker (2011)
  • Author(s): Yajing Shen, Masahiro Nakajima, Seiji Kojima, Michio Homma, Toshio Fukuda
  • Journal Title: Biochemical and Biophysical Research Communication Volume: Vol.409, No.2 Pages: 160-165
  • Peer Reviewed
• [Journal Article] Single cell adhesion force measurement for cell viability identification by using AFM cantilever based micro putter (2011)
  • Author(s): Yajing Shen, Masahiro Nakajima, Seiji Kojima, Michio Homma, Masaru Kojima, Toshio Fukuda
  • Journal Title: Measurement science and technology Volume: Vol.22, No.11 Pages: 115802-115802
  • Peer Reviewed
• [Journal Article] Design and characterization of nanoknife with buffering beam for in situ single cell cutting (2011)
  • Author(s): Yajing Shen, Masahiro Nakajima, Zhan Yang, Seiji Kojima, Michio Homma, Toshio Fukuda
  • Journal Title: Nanotechnology Volume: Vol.22, No.30 Pages: 305701-305701
  • Peer Reviewed
• [Journal Article] Effect of ambient humidity on the strength of the adhesion force of single yeast cell inside environmental-SEM (2011)
  • Author(s): Yajing Shen, Masahiro Nakajima, Mohd Ridzuan Ahmad, Seiji Kojima, Michio Homma, Toshio Fukuda
  • Journal Title: Ultramicroscopy Volume: Vol.111, No.8 Pages: 1176-1183
  • Peer Reviewed
• [Journal Article] Buckling Nanoneedle for Characterizing Single Cells Mechanics inside Environmental SEM (2011)
  • Author(s): Mohd Ridzuan Ahmad, Masahiro Nakajima, Seiji Kojima, Michio Homma, Toshio Fukuda
  • Journal Title: IEEE Transactions on Nanotechnology Volume: Vol.10 Issue 2 Pages: 226-236
  • Peer Reviewed
• [Journal Article] Microchip device for measurement of body volume of C.elegans as bioindicator application (2011)
  • Author(s): Jaehoon Jung, Masahiro Nakajima, Masaru Kojima, Katsutoshi Ooe, Toshio Fukuda
  • Journal Title: Journal of Micro-Nano Mechatronics Volume: 7 Issue: 1-3 Pages: 3-11
  • DOI: 10.1007/s12213-011-0036-7
  • Peer Reviewed
• [Journal Article] Study of the time effect on the strength of cell-cell adhesion force by a novel nano-picker (2011)
  • Author(s): Yajing Shen, Masahiro Nakajima, Seiji Kojima, Michio Homma, Toshio Fukuda
  • Journal Title: Biochemical and Biophysical Research Communication Volume: 409 Issue: 2 Pages: 160-165
  • DOI: 10.1016/j.bbrc.2011.04.019
  • Peer Reviewed
• [Presentation] Microfluidic device for Coincident Multi-exposure test on C. elegans (2014)
  • Author(s): Jaehoon JUNG, Masahiro NAKAJIMA, Masaru TAKEUCHI, Qiang HUANG, and Toshio FUKUDA
  • Organizer: International Symposium on Micro-Nano Mechatronics and Human Science (MHS2013)
  • Place of Presentation: 名古屋
• [Presentation] Microfluidic device for Coincident Multi-exposure test on C. elegans (2013)
  • Author(s): Jahoon Jung, Masahiro Nakajima, Masaru Takeuchi, Qiang Huang, Toshio Fukuda
  • Organizer: Proceedings 2013 International Symposium on Micromechatronics and Human Science
  • Place of Presentation: (60-63, 査読有)
• [Presentation] Real-Time Measurement of the Adhesion Force by Hybrid System of ESEM and AFM Cantilever (2013)
  • Author(s): Yajing Shen, Masahiro Nakajima, Zhan Yang, Qiang Huang, Toshio Fukuda
  • Organizer: Proceedings 13th IEEE International Conference on Nanotechnology(IEEE-Nano 2013)
  • Place of Presentation: (325-328, 査読有)
• [Presentation] Micro Fluidic Device to Analyze the Effect of Cadmium on Caenorhabditis elegans (2012)
  • Author(s): Jaehoon Jung, Masahiro Nakajima, Hirotaka Tajima, Toshio Fukuda
  • Organizer: Proceedings of the 2012 International Symposium on Micromechatronics and Human Science (MHS 2012)
  • Place of Presentation: (262-267, 査読有)
• [Presentation] Auto Nanomanipulation System for Single Cell Mechanical Property Characterization inside an Environmental SEM (2012)
  • Author(s): Yajing Shen, Masahiro Nakajima, Masaru Kojima, Toshio Fukuda
  • Organizer: Proceedings of the 2012 IEEE/RSJ International Conference on Intelligent Robotics and Systems (IROS 2012)
  • Place of Presentation: (646-651, 査読有)
• [Presentation] Micro Fluidic Device to Control the Position and to Analyze the Condition of C. elegans as a Bioindicator (2012)
  • Author(s): Jaehoon Jung, Masahiro Nakajima, Masaru Kojima, Hirotaka Tajima, Toshio Fukuda
  • Organizer: Proceedings of the 2012 IEEE/RSJ International Conference on Intelligent Robotics and Systems (IROS 2012)
  • Place of Presentation: (932-937, 査読有)
• [Presentation] Nano Needle with Buffering Beam for Single Cell Stiffness Measurement by Nanorobotic Manipulators inside ESEM (2012)
  • Author(s): Yajing Shen, Masahiro Nakajima, Zhan Yang, Michio Homma, Toshio Fukuda
  • Organizer: Proceedings of the 2012 12th IEEE Conference on Nanotechnology (IEEE-Nano 2012)
  • Place of Presentation: (4 pages, 1-4, 査読有)
• [Presentation] Development of the Auto Manipulation System towards the Single Cell Automatic Analysis inside an Environmental SEM (2012)
  • Author(s): Yajing Shen, Masahiro Nakajima, TaoYue, Seiji Kojima, Michio Homma, Toshio Fukuda
  • Organizer: Proceedings of the 2012 IEEE International Conference on Robotics and Automation (ICRA 2012)
  • Place of Presentation: (4594-4599, 査読有)
• [Presentation] Development of the Auto Manipulation System towards the Single Cell Automatic Analysis inside an Environmental SEM (2012)
  • Author(s): Yajing Shen, Masahiro Nakajima, Tao Yue, Seiji Kojima, Michio Homma, Toshio Fukuda
  • Organizer: Proceedings of the 2012 IEEE International Conference on Robotics and Automation (ICRA 2012)
  • Place of Presentation: Minnesota, USA
• [Presentation] Multi-slicing of C. elegans Tissue using Micro-Nanocutting Probe based on Nanomanipulation (2012)
  • Author(s): Masahiro Nakajima, Hirotaka Hida, Yajing Shen, Masaru Kojima, Kazuo Sato, Toshio Fukuda
  • Organizer: Proceedings of the 2012 12th IEEE Conference on Nanotechnology (IEEE-Nano 2012)
  • Place of Presentation: Birmingham, UK
• [Presentation] Nano Needle with Buffering Beam for Single Cell Stiffness Measurement by Nanorobotic Manipulators inside ESEM (2012)
  • Author(s): Yajing Shen, Masahiro Nakajima, Zhan Yang, Michio Homma, Toshio Fukuda
  • Organizer: Proceedings of the 2012 12th IEEE Conference on Nanotechnology (IEEE-Nano 2012)
  • Place of Presentation: Birmingham, UK
• [Presentation] Assembly and Evaluation of MWCNTs Probe Thermal Sensor by Nanorobotic Manipulation (2012)
  • Author(s): Zhan Yang, Masahiro Nakajima, Toshio Fukuda
  • Organizer: Proceedings of the 2012 12th IEEE Conference on Nanotechnology (IEEE-Nano 2012)
  • Place of Presentation: Birmingham, UK
• [Presentation] Local Nano-injection of Fluorescent Nano-beads inside C. elegans based on Nanomanipulation (2012)
  • Author(s): Masahiro Nakajima, Takahiro Hirano, Masaru Kojima, Naoki Hisamoto, Naoya Nakanishi, Hirotaka Tajima, Michio Homma, Toshio Fukuda
  • Organizer: Proceedings of the 2012 IEEE/RSJ International Conference on Intelligent Robotics and Systems (IROS 2012)
  • Place of Presentation: Vilamoura, Portugal
• [Presentation] Micro Fluidic Device to Control the Position and to Analyze the Condition of C. elegans as a Bioindicator (2012)
  • Author(s): Jaehoon Jung, Masahiro Nakajima, Masaru Kojima, Hirotaka Tajima, Toshio Fukuda
  • Organizer: Proceedings of the 2012 IEEE/RSJ International Conference on Intelligent Robotics and Systems (IROS 2012)
  • Place of Presentation: Vilamoura, Portugal
• [Presentation] Auto Nanomanipulation System for Single Cell Mechanical Property Characterization inside an Environmental SEM (2012)
  • Author(s): Yajing Shen, Masahiro Nakajima, Masaru Kojima, Toshio Fukuda
  • Organizer: Proceedings of the 2012 IEEE/RSJ International Conference on Intelligent Robotics and Systems (IROS 2012)
  • Place of Presentation: Vilamoura, Portugal
• [Presentation] Micro Fluidic Device to Analyze the Effect of Cadmium on Caenorhabditis elegans (2012)
  • Author(s): Jaehoon Jung, Masahiro Nakajima, Hirotaka Tajima, Toshio Fukuda
  • Organizer: Proceedings of the 2012 International Symposium on Micromechatronics and Human Science (MHS 2012)
  • Place of Presentation: Nagoya, Japan
• [Presentation] Local Injection Probe of Functional Micro-Nano Gel Tools into Caenorhabditis elegans (2012)
  • Author(s): Masahiro Nakajima, Naoya Nakanishi, Naoki Hisamoto, Hirotaka Tajima, Michio Homma, Toshio Fukuda
  • Organizer: Proceedings of the 2012 International Symposium on Micromechatronics and Human Science (MHS 2012)
  • Place of Presentation: Nagoya, Japan
• [Presentation] Selective Nano-injection using Nano-Probe based on Nanomanipulation under Hybrid Microscope (2011)
  • Author(s): Takahiro Hirano, Masahiro Nakajima, Masaru Kojima, Naoki Hisamoto, Michio Homma, Toshio Fukuda
  • Organizer: Proceedings of the 2011 International Symposium on Micromechatronics and Human Science (MHS 2011)
  • Place of Presentation: (216-221, 査読有)
• [Presentation] Method to Study the Single Cell's Time-variation Adhesion Strength during the Manipulation inside ESEM (2011)
  • Author(s): Yajing Shen, Masahiro Nakajima, Zoran Najdovski, Zhan Yang, Masaru Kojima, Seiji Kojima, Michio Homma, Toshio Fukuda
  • Organizer: Proceedings of the 2011 International Symposium on Micromechatronics and Human Science (MHS 2011)
  • Place of Presentation: (210-215, 査読有)
• [Presentation] Measurement of Body Volume of Live C. elegans by Microchip (2011)
  • Author(s): Jaehoon Jung, Masahiro Nakajima, Masaru Kojima, Toshio Fukuda
  • Organizer: Proceedings of the 2011 International Symposium on Micromechatronics and Human Science (MHS 2011)
  • Place of Presentation: (204-209, 査読有)
• [Presentation] Microchip Analysis Device for Fixed Caenorhabditis elegans by Tapered Micro channel (2011)
  • Author(s): Jaehoon Jung, Masahiro Nakajima, Masaru Kojima, Toshio Fukuda
  • Organizer: Proceedings of the SICE Annual Conference 2011
  • Place of Presentation: (2421-2426, 査読有)
• [Presentation] Evaluation of Nano-Knife's Edge Angle for Single Cell Cutting by Using Nanorobotic Manipulators Inside ESEM (2011)
  • Author(s): Yajing Shen, Masahiro Nakajima, Zhang Yang, Seiji Kojima, Michio Homma, Toshio Fukuda, Masaru Kojima
  • Organizer: Proceedings of the 2011 11th IEEE Conf. on Nanotechnology (IEEE-Nano 2011)
  • Place of Presentation: (Vol.1, 155-160, 査読有)
• [Presentation] Direct Nano-injection Method by Nanoprobe Insertion based on E-SEM Nanorobotic Manipulation under Hybrid Microscope (2011)
  • Author(s): Masahiro Nakajima, Takanori Hirano, Masaru Kojima, Naoki Hisamoto, Michio Homma, Toshio Fukuda
  • Organizer: Proceedings of the 2011 IEEE International Conference on Robotics and Automation (ICRA 2011)
  • Place of Presentation: (4139-4144, 査読有)
• [Presentation] Characterization of Oscillating Nano Knife for Single Cell Cutting by Nanorobotic Manipulation System inside ESEM (2011)
  • Author(s): Yajing Shen, Masahiro Nakajima, Seiji Kojima, Michio Homma, Toshio Fukuda
  • Organizer: Proceedings of the 2011 IEEE International Conference on Robotics and Automation (ICRA 2011)
  • Place of Presentation: (4133-4138, 査読有)
• [Presentation] Measurement for Viability Identification using Nanorobotic Manipulation System inside ESEM (2011)
  • Author(s): Yajing Shen, Masahiro Nakajima, Seiji Kojima, Michio Homma, Toshio Fukuda
  • Organizer: Proceedings of 2011 IEEE International Conference on Nano/Micro Engineered and Molecular Systems (IEEE-NEMS 2011)
  • Place of Presentation: (944-947, 査読有)
• [Presentation] Characterization of Oscillating Nano Knife for Single Cell Cutting by Nanorobotic Manipulation System inside ESEM (2011)
  • Author(s): Yajing Shen, Masahiro Nakajima, Seiji Kojima, Michio Homma, Toshio Fukuda
  • Organizer: Proceedings of the 2011 IEEE International Conference on Robotics and Automation (ICRA2011), pp.4133-4138
  • Place of Presentation: Shanghai, China
• [Presentation] Direct Nano-injection Method by Nanoprobe Insertion based on E-SEM Nanorobotic Manipulation under Hybrid Microscope (2011)
  • Author(s): Masahiro Nakajima, Takanori Hirano, Masaru Kojima, Naoki Hisamoto, Michio Homma, Toshio Fukuda
  • Organizer: Proceedings of the 2011 IEEE International Conference on Robotics and Automation (ICRA2011), pp.4133-4138
  • Place of Presentation: Shanghai, China
• [Presentation] Evaluation of Nano-Knife's Edge Angle for Single Cell Cutting by Using Nanorobotic Manipulators Inside ESEM (2011)
  • Author(s): Yajing Shen, Masahiro Nakajima, Zhang Yang, Seiji Kojima, Michio Homma, Toshio Fukuda, Masaru Kojima
  • Organizer: Proceedings of the 2011 11th IEEE Conf.on Nanotechnology (IEEE-Nano 2011), Vol.1, pp.155-160
  • Place of Presentation: Portland, USA
• [Presentation] Microchip Analysis Device for Fixed Caenorhabditis elegans by Tapered Micro channel (2011)
  • Author(s): Jaehoon Jung, Masahiro Nakajima, Masaru Kojima, Toshio Fukuda
  • Organizer: Proceedings of the SICE Annual Conference 2011, pp.2421-2426
  • Place of Presentation: Tokyo, Japan
• [Presentation] Nanotool Exchanger System using Low-melting Metal under Environmental SEM (2011)
  • Author(s): Masahiro Nakajima, Takuya Kawamoto, Masaru Kojima, Toshio Fukuda
  • Organizer: Proceedings of the 2011 International Symposium on Micromechatronics and Human Science (MHS 2011), pp.69-74
  • Place of Presentation: Nagoya, Japan
• [Presentation] Method to Study the Single Cell's Time-variation Adhesion Strength during the Manipulation inside ESEM (2011)
  • Author(s): Yajing Shen, Masahiro Nakajima, Zoran Najdovski, Zhan Yang, Masaru Kojima, Seiji Kojima, Michio Homma, Toshio Fukuda
  • Organizer: Proceedings of the 2011 International Symposium on Micromechatronics and Human Science (MHS 2011), pp.210-215
  • Place of Presentation: Nagoya, Japan
• [Presentation] Selective Nano-injection using Nano-Probe based on Nanomanipulation under Hybrid Microscope (2011)
  • Author(s): Takahiro Hirano, Masahiro Nakajima, Masaru Kojima, Naoki Hisamoto, Michio Homma, Toshio Fukuda
  • Organizer: Proceedings of the 2011 International Symposium on Micromechatronics and Human Science (MHS 2011), pp.216-221
  • Place of Presentation: Nagoya, Japan
• [Book] Micro-Nanorobotic Manipulation Systems and Their Applications (2013)
  • Author(s): Toshio Fukuda, Fumihito Arai, Masahiro Nakajima
  • Publisher: Springer
• [Book] 「リアルタイム計測による生命現象の解析」第2章環境制御型電子顕微鏡内ナノマニピュレーション技術(監修村田静昭) (2011)
  • Author(s): 中島正博, 福田敏男, アハマド モハマド リズワン
  • Publisher: シーエムシー出版刊
• [Remarks]
  • URL: http://www.mein.nagoya-u.ac.jp/indexj.html
• [Patent(Industrial Property Rights)] 微生物の電気化学的特性の解析用マイクロチップデバイス (2011)
  • Inventor(s): 福田敏男,中島正博,小嶋勝
  • Industrial Property Rights Holder: 福田敏男,中島正博,小嶋勝
  • Industrial Property Number: 2011-231134
  • Filing Date: 2011-09-16