| Budget Amount *help |
¥49,140,000 (Direct Cost: ¥37,800,000、Indirect Cost: ¥11,340,000)
Fiscal Year 2013: ¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000)
Fiscal Year 2012: ¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2011: ¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
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| Research Abstract |
Specific targets were detected by short DNA and RNA aptamers immobilized on diamond. 1) HIV-TAT protein(target) and platelet derived growth factor (PDGF) were detected by their aptamers using two methods such as photonic method based on fluorescence dye and potentiometric method based on field effect transistor (FET). The bonding between aptamer and their targets were discussed by the two different detecting mechanisms. 2) Adenosine-triphosphate (ATP) has been clearly detected by without-marker on ATP, but with-marker on its aptamer. It shows the possibility of label free aptamer sensor, 3) PDGF were selectively detected by different transistors in FET arrays which are placed at the bottom of micro flow channels. It leads to the basic technology for integrated micro bio-systems.
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