| Budget Amount *help |
¥48,620,000 (Direct Cost: ¥37,400,000、Indirect Cost: ¥11,220,000)
Fiscal Year 2014: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
Fiscal Year 2013: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
Fiscal Year 2012: ¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000)
Fiscal Year 2011: ¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000)
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| Outline of Final Research Achievements |
Analysis of the mechanism of undifferentiated state maintenance is one of the most important research subjects for regenerative therapy and cell medicine. This study was aimed at the real time analysis of dynamic expression of 4 genes; Oct3/4, Sox2, Cdx2, and Nanog, in living single-cells of mouse ES cell. The gene expression regulating vectors, gene product proteins, and gene expression visualized cell lines were developed for the study. Vectors and proteins were introduced into single ES cells of novel cell lines by femtoinjection and their gene expressions were analyzed by time-lapse imaging. Typical experimental results were the relationship between Nanog and Cdx2 expressions and the relationship between Oct3/4 protein quantity and Oct3/4 gene expression.
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