How cortical microtubule patterns are established?

• Project/Area Number: 23247008
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: HASHIMOTO Takashi 奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (80180826)
• Project Period (FY): 2011-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥48,360,000 (Direct Cost: ¥37,200,000、Indirect Cost: ¥11,160,000); Fiscal Year 2014: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000); Fiscal Year 2013: ¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000); Fiscal Year 2012: ¥12,220,000 (Direct Cost: ¥9,400,000、Indirect Cost: ¥2,820,000); Fiscal Year 2011: ¥14,430,000 (Direct Cost: ¥11,100,000、Indirect Cost: ¥3,330,000)
• Keywords: 微小管 / チューブリン / 重合 / アラビドプシス / 植物 / γチューブリン / 重合開始 / 重合核複合体 / MOZART

Outline of Final Research Achievements

It is not well known how microtubules are assembled into higher order structures and form diverse array patterns in acentrosomal plant cells. To ultimately construct ordered microtubule patterns in vitro, this study established an efficient purification method that purify polymerization-competent tubulins from the model plant Arabidopsis thaliana, and analyzed detailed nucleation patterns of cortical microtubules in Arabidopsis epidermal cells.

Research Products

• [Journal Article] GCP-WD mediates g-TuRC recruitment and the geometry of microtubule nucleation in the acentrosomla interphase arrays of Arabidopsis. (2014)
  • Author(s): Walia et al.
  • Journal Title: Curr. Biol. Volume: 24 Issue: 21 Pages: 2548-2555
  • DOI: 10.1016/j.cub.2014.09.013
  • Peer Reviewed
• [Journal Article] Dissecting the cellular functions of plant microtubules using mutant tubulins. (2013)
  • Author(s): T. Hashimoto
  • Journal Title: Cytoskeleton Volume: 70 Issue: 4 Pages: 191-200
  • DOI: 10.1002/cm.21099
  • Peer Reviewed
• [Journal Article] A ring for all: -tubulin-containing nucleation complexes in acentrosomal plant microtubule arrays (2013)
  • Author(s): T. Hashimoto
  • Journal Title: Curr. Opin. Plant Biol. Volume: 16 Issue: 6 Pages: 698-703
  • DOI: 10.1016/j.pbi.2013.09.002
  • Peer Reviewed
• [Journal Article] Purification and characterization of novel microtubule-associated proteins from Arabidopsis cell suspension cultures. (2013)
  • Author(s): Takahiro Hamada, Nahoko Nagasaki Tekeuchi, Takehide Kato, Masayuki Fujiwara, Seiji Sonobe, Yoichiro Fukao, Takashi Hashimoto
  • Journal Title: Plant Physiol. Volume: 163 Issue: 4 Pages: 1804-1816
  • DOI: 10.1104/pp.113.225607
  • Peer Reviewed
• [Journal Article] RNA Processing Bodies, Peroxisomes, Golgi Bodies, Mitochondria, and ER Tubule Junctions Frequently Pause at Cortical Microtubules (2012)
  • Author(s): Takahiro Hamada, Motoki Tominaga, Takeshi Fukaya, Masayoshi Nakamura, Akihiko Nakano, Yuichiro Watanabe, Takashi Hashimoto, Tobias I.Baskin
  • Journal Title: Plant and Cell Physiology Volume: 53 Issue: 4 Pages: 699-708
  • DOI: 10.1093/pcp/pcs025
  • Peer Reviewed
• [Journal Article] Arabidopsis GCP3-INTERACTING PROTEIN1/MOZAET1 is an integral component of the Y-tubulin-containing microtubule nucleating complex (2012)
  • Author(s): Nakamura M, Yagi N, Kato T, Fujita S, Kawashima N, Ehrhardt DW, Hashimoto T
  • Journal Title: The Plant Journal Volume: (In Press) Issue: 2 Pages: 216-225
  • DOI: 10.1111/j.1365-313x.2012.04988.x
  • Peer Reviewed
• [Journal Article] Microtubule and cell shape determination (2011)
  • Author(s): T.Hashimoto
  • Journal Title: The Plant Cytoskeleton Pages: 245-257
  • DOI: 10.1007/978-1-4419-0987-9_11
  • ISBN: 9781441909862, 9781441909879
• [Presentation] Stress-induce destabilization of cortical microtubules is mediated by tubulin kinase (2015)
  • Author(s): Takashi Hashimoto
  • Organizer: Front Lines of Plant Cell Wall Research
  • Place of Presentation: 東大寺総合文化センター、奈良県奈良市
  • Year and Date: 2015-03-20 – 2015-03-21
  • Invited
• [Presentation] ゼニゴケを用いたストレス誘導性の表層微小管脱重合機構の解析 (2015)
  • Author(s): 高橋英之
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京農業大学世田谷キャンパス、東京都世田谷区
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] TOG カラムを用いた植物培養細胞からのチューブリン精製 (2015)
  • Author(s): 堀田 崇
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京農業大学世田谷キャンパス、東京都世田谷区
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] Functional analysis of novel Arabidopsis proteins that associate with microtubule nucleation sites. (2014)
  • Author(s): N. Yagi, T. Hamada, M. Nakamura, M. Kawaguchi, T. Kato and T. Hashimoto
  • Organizer: 日本植物生理学会
  • Place of Presentation: 富山大学、富山県富山市
• [Presentation] シロイヌナズナ新規微小管結合タンパク質ファミリーの機能解析
  • Author(s): 長崎菜穂子
  • Organizer: 日本植物生理学会年会
  • Place of Presentation: 岡山大学
• [Presentation] 微小管形成開始部位に局在するシロイヌナズナ新規MAPsの機能解析
  • Author(s): 加藤壮英
  • Organizer: 日本植物生理学会年会
  • Place of Presentation: 岡山大学
• [Presentation] Dual labeling of microtubules and nucleation complexes reveals detailed array assembly processes in Arabidopsis cells
  • Author(s): 八木慎宜
  • Organizer: 日本植物生理学会年会
  • Place of Presentation: 岡山大学
• [Presentation] atypical kinase PHS1は高浸透圧条件下において微小管の脱重合を促進する
  • Author(s): 藤田智史
  • Organizer: 日本植物生理学会年会
  • Place of Presentation: 岡山大学
• [Presentation] Organization of cortical microtubule arrays in Arabidopsis
  • Author(s): Takashi Hashimoto
  • Organizer: 10th International Congress of Plant Molecular Biology
  • Place of Presentation: Cheju, Korea
  • Invited
• [Presentation] Organization of cortical microtubule arrays in Arabidopsis
  • Author(s): Takashi Hashimoto
  • Organizer: EMBL Workshop "Microtubules"
  • Place of Presentation: Heidelberg, Germany
  • Invited