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The morphogenesis and functional differentiation of endoplasmic reticulum in plant cells

Research Project

Project/Area Number 23247009
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionUniversity of Hyogo

Principal Investigator

SHIMMEN Teruo  兵庫県立大学, 生命理学研究科, 教授 (80114510)

Co-Investigator(Kenkyū-buntansha) 横田 悦雄  兵庫県立大学, 生命理学研究科, 助教 (80212299)
Co-Investigator(Renkei-kenkyūsha) 西村 いくこ  京都大学, 理学研究科, 教授 (00241232)
園部 誠司  兵庫県立大学, 生命理学研究科, 准教授 (30197024)
Project Period (FY) 2011-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2014: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2013: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2012: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Keywords小胞体 / RHD3 / GTP / シロイヌナズナ / ミオシン / RHD3 / ダイナソー / アトラスチン / GTP / タバコ培養細胞 / カルシウムイオン / エクオリン / レティキュロン / アクチン / ビリン
Outline of Final Research Achievements

We investigated the role of RHD3, a plant homologue of atlastin GTPase, on the membrane fusion of endoplasmic reticulum. Based on analyses of the membrane fusion and the tubular formation in vitro of endoplasmic reticulum isolated from Arabidopsis seedling or cultured cell, RHD3 was revealed to be involved in GTP-dependent membrane fusion of endoplasmic reticulum. Furthermore, the biochemical approach with the chemical cross-linkers showed that the membrane fusion of endoplasmic reticulum was progressed through the formation of RHD3 oligomer by the association with GTP.

Report

(5 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (31 results)

All 2015 2014 2013 2012 2011 Other

All Journal Article (11 results) (of which Peer Reviewed: 11 results) Presentation (17 results) (of which Invited: 1 results) Remarks (3 results)

  • [Journal Article] Cytoplasmic streaming velocity as a plant size determinant2013

    • Author(s)
      M. Tominaga, A. Kimura, E. Yokota, T. Hamaguchi, T. Shimmen, K. Yamamoto, A. Nakano, and K. Ito
    • Journal Title

      Dev. Cell

      Volume: 27 Issue: 3 Pages: 45-352

    • DOI

      10.1016/j.devcel.2013.10.005

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Reconstruction of active regular motion in amoeba extract: dynamic cooperation between sol and gel states.2013

    • Author(s)
      Nishigami Y, Ichikawa M, Kazama T, Kobayashi R, Shimmen T, Yoshikawa K, Sonobe S
    • Journal Title

      PLoS One

      Volume: 5;8(8) Issue: 8 Pages: e70317-e70317

    • DOI

      10.1371/journal.pone.0070317

    • NAID

      120005312151

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ion gradients in xylem exudate and guttation fluid related to tissue ion levels along primary leaves of barley.2013

    • Author(s)
      Nagai Makiko, Ohnishi Miwa, Uehara Takeo, Yamagami Mutsumi, Miura Eiko, Kamakura Mai, Kitamura Akira, Sakaguchi Shuichi, Sakamoto Wataru, Shimmen Teruo, Fukaki Hidehiro, Reid Rob, J., Furukawa Akio and Mimura Tetsuro
    • Journal Title

      Plant, Cell & Environment

      Volume: 36 Issue: 10 Pages: 1826-1837

    • DOI

      10.1093/pcp/pct107

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ion gradients in xylem exudate and guttation fluid related to tissue ion levels along primary leaves of barley.2013

    • Author(s)
      Nagai M., Ohnishi M., Uehara T., Yamagami M., Miura E., Kamakura M., et al.
    • Journal Title

      Plant, Cell and Environment

      Volume: 36 Issue: 10 Pages: 1826-1837

    • DOI

      10.1111/pce.12090

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transduction of pressure signal to electrical signal upon sudden increase in turgor pressure in Chara corallina.2013

    • Author(s)
      Shimmen T, Ogata K.
    • Journal Title

      Journal of Plant Research

      Volume: 126(3) Issue: 3 Pages: 439-446

    • DOI

      10.1007/s10265-012-0537-z

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Studies on conjugation of Spirogyra using monoclonal culture2012

    • Author(s)
      H. Ikegaya, T. Nakase, K. Iwata, H. Tsuchida, S. Sonobe, T. Shimmen
    • Journal Title

      Journal of Plant Research

      Volume: 125 Issue: 3 Pages: 457-464

    • DOI

      10.1007/s10265-011-0457-3

    • NAID

      10030652893

    • Related Report
      2012 Annual Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Further electrophysiological studies on cellular effect of herbicide, bromoxynil, using chracean cells2012

    • Author(s)
      T.Shimmen
    • Journal Title

      Journal of Plant Research

      Volume: (印刷中) Issue: 6 Pages: 749-754

    • DOI

      10.1007/s10265-012-0487-5

    • Related Report
      2012 Annual Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Calcium-induced mechanical change in the neck domain alters the activity of plant myosin XI2012

    • Author(s)
      M. Tominaga, H. Kojima, E. Yokota, R. Nakamori, M. Anson, T. Shimmen, K. Oiwa
    • Journal Title

      The Journal of Biological Chemistry

      Volume: 287 Pages: 30711-30718

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Myosin XI-dependent formation of tubular structures from endoplasmic reticulum isolated from tobacco cultured cells, BY-22011

    • Author(s)
      E. Yokota, H. Ueda, K. Hashimoto, H. Orii, T. Shimada, I. Hara-Nishimura, T. Shimmen
    • Journal Title

      Plant Physiol

      Volume: 156 Issue: 1 Pages: 129-143

    • DOI

      10.1104/pp.111.175018

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The myosin ATPase inhibitor, 2,3-butanedione 2-monoxime, prevents protein secretion by the basidiomycete Coprinopsis cinerea2011

    • Author(s)
      Hashimoto, K., Yokota, E., Shimmen, T., and Yoshida, M.
    • Journal Title

      Biotechnol. Lett.

      Volume: 33 Issue: 4 Pages: 769-775

    • DOI

      10.1007/s10529-010-0497-0

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Involvement of protein synthesis in recovery from refractory period of electrical depolarization induced by osmotic stimulation in Chara coralline2011

    • Author(s)
      T.Shimmen
    • Journal Title

      Journal of Plant Research

      Volume: 124 Issue: 5 Pages: 639-644

    • DOI

      10.1007/s10265-010-0391-9

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] アクチン結合タンパク質ビリンの活性はリン脂質により修飾される2015

    • Author(s)
      横田悦雄
    • Organizer
      第56回日本植物生理学会年会
    • Place of Presentation
      東京農業大学世田谷キャンパス(東京都・世田谷区)
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] オオシャジクモにおける圧受容機構2014

    • Author(s)
      新免輝男
    • Organizer
      日本植物学会第78回大会
    • Place of Presentation
      明治大学生田キャンパス(神奈川県川崎市)
    • Year and Date
      2014-09-12 – 2014-09-14
    • Related Report
      2014 Annual Research Report
  • [Presentation] 速度改変型キメラミオシンXIによる原形質流動速度変化が植物サイズに及ぼす影響2013

    • Author(s)
      富永 基樹, 木村 篤司, 横田 悦雄, 原口 武士, 新免 輝男, 山本 啓一, 中野 明彦, 伊藤 光二
    • Organizer
      日本細胞生物学会第65回大会
    • Place of Presentation
      ウインクあいち(愛知県名古屋市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Characeae as a model system for electrophysiological studies on intercellular communication.2013

    • Author(s)
      Teruo Shimmen
    • Organizer
      Annual Meeting of the Society of Plant Signaling and Behavior 2013
    • Place of Presentation
      バンクーバー(カナダ)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] イカダケイソウの滑走運動機構2011

    • Author(s)
      山岡望海
    • Organizer
      2011年日本植物学会近畿支部大会
    • Place of Presentation
      キャンパスプラザ京都(京都府)
    • Year and Date
      2011-12-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] オオシャジクモにおける酸化的リン酸化脱共役剤の脱分極作用2011

    • Author(s)
      新免輝男
    • Organizer
      2011年日本植物学会近畿支部大会
    • Place of Presentation
      キャンパスプラザ京都(京都府)
    • Year and Date
      2011-12-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] 植物細胞におけるミオシン依存的な小胞体流動とアクチン繊維束の組織化2011

    • Author(s)
      上田晴子
    • Organizer
      日本生化学会第84回大会
    • Place of Presentation
      京都国際会館(京都府)(招待講演)
    • Year and Date
      2011-09-21
    • Related Report
      2011 Annual Research Report
  • [Presentation] 接合菌類ヒゲカビの原形質流動を担うミオシンの同定2011

    • Author(s)
      横田悦雄
    • Organizer
      日本植物学会第75回大会
    • Place of Presentation
      東京大学駒場キャンパス(東京都)
    • Year and Date
      2011-09-18
    • Related Report
      2011 Annual Research Report
  • [Presentation] イカダケイソウの滑走運動に関するアクチンの役割2011

    • Author(s)
      山岡望海
    • Organizer
      日本藻類学会第32回大会
    • Place of Presentation
      東京海洋大学(東京都)
    • Year and Date
      2011-05-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] 速度改変型キメラミオシンXIの発現により明らかになってきた原形質流動の機構と制御

    • Author(s)
      富永基樹、伊藤光二、原田武士、横田悦雄、新免輝男、山本啓一、中野明彦
    • Organizer
      第55回日本生理学会年会
    • Place of Presentation
      富山大学(富山県富山市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 植物サイズ制御における原形質流動の機能およびミオシンXIメンバーの役割分担

    • Author(s)
      富永 基樹、伊藤 光二、原口 武士、横田悦雄、新免 輝男、山本啓一、中野 明彦
    • Organizer
      日本植物学会第77回大会
    • Place of Presentation
      北海道大学(北海道札幌市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] オオシャジクモにおける圧シグナルの電気シグナルへの変換

    • Author(s)
      新免輝男
    • Organizer
      日本植物学会第76回大会
    • Place of Presentation
      兵庫県立大学・書写キャンパス(兵庫県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] アオミドロの障害における細胞質の集積機構

    • Author(s)
      堀川千尋、岩田和佳、新免輝男
    • Organizer
      日本植物学会第76回大会
    • Place of Presentation
      兵庫県立大学・書写キャンパス(兵庫県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] タバコ培養細胞における小胞体輸送を担うミオシン

    • Author(s)
      横田悦雄、上田晴子、西村いくこ、新免輝男
    • Organizer
      日本植物学会第76回大会
    • Place of Presentation
      兵庫県立大学・書写キャンパス(兵庫県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 小胞体運動の解析から見えてきたミオシンとアクチンの意外な関係

    • Author(s)
      上田晴子、横田悦雄、朽名夏麿、嶋田知生、田村謙太郎、新免輝男、西村いくこ
    • Organizer
      日本植物学会第76回大会
    • Place of Presentation
      兵庫県立大学・書写キャンパス(兵庫県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 分子レベルから眺める原形質流動

    • Author(s)
      富永基樹、伊藤光二、小嶋寛明、横田悦雄、山本啓一、新免輝男、大岩和弘、中野明彦
    • Organizer
      日本植物学会第76回大会
    • Place of Presentation
      兵庫県立大学・書写キャンパス(兵庫県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 小胞体運動に関わる小胞体タンパク質の解析

    • Author(s)
      上田晴子、横田悦雄、朽名夏麿、真野昌二、嶋田知生、田村謙太郎、新免輝男、西村幹夫、西村いくこ
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山大学・津島キャンパス (岡山県)
    • Related Report
      2012 Annual Research Report
  • [Remarks] 兵庫県立大学・大学院生命理学研究科・分子機械学講座

    • URL

      http://www.sci.u-hyogo.ac.jp/life/biomecha/index-j.html

    • Related Report
      2014 Annual Research Report
  • [Remarks] 兵庫県立大学・大学院生命理学研究科・分子機械学講座ホームページ

    • URL

      http://www.sci.u-hyogo.ac.jp/life/biomecha/index-j.html

    • Related Report
      2013 Annual Research Report
  • [Remarks] 兵庫県立大学・生命理学研究科・分子機械学講座ホームページ

    • URL

      http://www.sci.u-hyogo.ac.jp/life/biomecha/index-j.html

    • Related Report
      2012 Annual Research Report

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Published: 2013-05-15   Modified: 2019-07-29  

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