Studies on the assembly of super-molecular complex by synthetic approach
| Project/Area Number |
23247013
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (A)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Structural biochemistry
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
UEDA Takuya 東京大学, 新領域創成科学研究科, 教授 (80184927)
|
|---|
| Project Period (FY) |
2011-04-01 – 2014-03-31
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥48,360,000 (Direct Cost: ¥37,200,000、Indirect Cost: ¥11,160,000)
Fiscal Year 2013: ¥14,950,000 (Direct Cost: ¥11,500,000、Indirect Cost: ¥3,450,000)
Fiscal Year 2012: ¥14,950,000 (Direct Cost: ¥11,500,000、Indirect Cost: ¥3,450,000)
Fiscal Year 2011: ¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
|
|---|
| Keywords | リボソーム / 無細胞蛋白質合成系 / リポソーム / シャペロン / ＡＴＰ合成酵素 / トランスロコン / 膜タンパク質 / 自己組織化 / 生体外蛋白質合成系 / 膜蛋白質複合体 / フォールディング / 膜蛋白質 / 蛋白質複合体 / ATPase / トランスコロン / 膜透過機構 |
|---|
| Research Abstract |
Most of proteins are able to express function by forming a complex of subunit.However, the formation process of a complex in the cell is only poorly understood. In this project, using a cel-free translation system constituted with purified translation factors, PURE system, complex formation processes were reconstructed in the test-tube. As a result, we succeeded in the synthesis of the ATP synthase, which is formed from22 polypeptides and translocon composed of three polypeptides onto the cell membrane. the reconstruction of small subunit of the ribosome (30S) with high efficiency was also successfully achieved form a 16SrRNA and of 20 ribosomal proteins in the presence of the factors involved in biosynthesis of 30S ribosome subunit.
|
Report
(4 results)
Research Products
(89 results)
-
-
-
-
-
[Journal Article] Unbiased Tracking of the Progression of mRNA and Protein Synthesis in Bulk and in Liposome-Confined Reactions2013
Author(s)
van Nies P, Nourian Z, Kok M, van Wijk R, Moeskops J, Westerlaken I, Poolman JM, Eelkema R, van Esch JH, Kuruma Y, Ueda T, Danelon C
-
Journal Title
Chembiochem
Volume: 14(15)
Pages: 1963-6
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Journal Article] Single molecule imaging of the trans-translation entry process via anch oring of the tagged ribosome.2011
Author(s)
Zhou, Z-P., Shimizu, Y., Tadakuma, H.*, Taguchi, H., Ito, K., Ueda, T.
-
Journal Title
J. Biochem.
Volume: 149
Issue: 5
Pages: 609-618
DOI
NAID
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
