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Studies on the assembly of super-molecular complex by synthetic approach

Research Project

Project/Area Number 23247013
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Structural biochemistry
Research InstitutionThe University of Tokyo

Principal Investigator

UEDA Takuya  東京大学, 新領域創成科学研究科, 教授 (80184927)

Project Period (FY) 2011-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥48,360,000 (Direct Cost: ¥37,200,000、Indirect Cost: ¥11,160,000)
Fiscal Year 2013: ¥14,950,000 (Direct Cost: ¥11,500,000、Indirect Cost: ¥3,450,000)
Fiscal Year 2012: ¥14,950,000 (Direct Cost: ¥11,500,000、Indirect Cost: ¥3,450,000)
Fiscal Year 2011: ¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Keywordsリボソーム / 無細胞蛋白質合成系 / リポソーム / シャペロン / ATP合成酵素 / トランスロコン / 膜タンパク質 / 自己組織化 / 生体外蛋白質合成系 / 膜蛋白質複合体 / フォールディング / 膜蛋白質 / 蛋白質複合体 / ATPase / トランスコロン / 膜透過機構
Research Abstract

Most of proteins are able to express function by forming a complex of subunit.However, the formation process of a complex in the cell is only poorly understood. In this project, using a cel-free translation system constituted with purified translation factors, PURE system, complex formation processes were reconstructed in the test-tube. As a result, we succeeded in the synthesis of the ATP synthase, which is formed from22 polypeptides and translocon composed of three polypeptides onto the cell membrane. the reconstruction of small subunit of the ribosome (30S) with high efficiency was also successfully achieved form a 16SrRNA and of 20 ribosomal proteins in the presence of the factors involved in biosynthesis of 30S ribosome subunit.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (89 results)

All 2014 2013 2012 2011 Other

All Journal Article (35 results) (of which Peer Reviewed: 35 results) Presentation (50 results) (of which Invited: 1 results) Book (3 results) Remarks (1 results)

  • [Journal Article] PURE ribosome display and its application in antibody technology2014

    • Author(s)
      Kanamori T, Fujino Y, Ueda T
    • Journal Title

      Biochim Biophys Acta. Pii

      Volume: S1570-9639(14) Issue: 11 Pages: 93-4

    • DOI

      10.1016/j.bbapap.2014.04.007

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Signal Recognition Particle and SecA Cooperate during Export of Secretory Proteins with Highly Hydrophobic Signal Sequences2014

    • Author(s)
      Zhou Y, UedaT, MullerM
    • Journal Title

      PLoS One

      Volume: 9(4) Issue: 4 Pages: e92994-e92994

    • DOI

      10.1371/journal.pone.0092994

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Role of Positions e and g in the Fibrous Assembly Formation of an Amphipathic alpha-Helix-Forming Polypeptide2014

    • Author(s)
      Takei, T.
    • Journal Title

      Biopolymers

      Volume: in press Issue: 3 Pages: 260-72

    • DOI

      10.1002/bip.22479

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The PURE system for protein production2014

    • Author(s)
      Shimizu, Y.
    • Journal Title

      Methods Mol Biol

      Volume: 1118 Pages: 275-284

    • DOI

      10.1007/978-1-62703-782-2_19

    • ISBN
      9781627037815, 9781627037822
    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Unbiased Tracking of the Progression of mRNA and Protein Synthesis in Bulk and in Liposome-Confined Reactions2013

    • Author(s)
      van Nies P, Nourian Z, Kok M, van Wijk R, Moeskops J, Westerlaken I, Poolman JM, Eelkema R, van Esch JH, Kuruma Y, Ueda T, Danelon C
    • Journal Title

      Chembiochem

      Volume: 14(15) Pages: 1963-6

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Direct monitoring of initiation factor dynamics through formation of 30S and 70S translation-initiation complexes on a quartz crystal microbalance2013

    • Author(s)
      Takahashi S, Isobe H, Ueda T, Okahata Y
    • Journal Title

      Chemistry

      Volume: 19(21) Pages: 6807-16

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Translation Enhancer Improves the Ribosome Liberation from Translation Initiation2013

    • Author(s)
      Takahashi S, Furusawa H, Ueda T, Okahata Y
    • Journal Title

      J Am Chem Soc.

      Volume: 135(35) Pages: 13096-106

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Nuclear Respiratory Factor 2β (NRF-2β) Recruits NRF-2α to the Nucleus by Binding to Importin-α : β via an Unusual Monopartite-Type Nuclear Localization Signal2013

    • Author(s)
      Hayashi R, Takeuchi N, Ueda T
    • Journal Title

      J Mol Biol

      Volume: 425(18) Pages: 3536-48

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Crystallization and preliminary X-ray analysis of peptidyl-tRNA hydrolase from Thermus thermophilus HB82013

    • Author(s)
      Matsumoto A, Shimizu Y, Takemoto C, Ueda T,UchiumiT, Ito K
    • Journal Title

      Acta Crystallogr Sect F Struct Biol Cryst Commun

      Volume: 69(Pt 3) Pages: 332-5

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Human G-proteins, ObgH1 and Mtg1, associate with the large mitochondrial ribosome subunit and are involved in translation and assembly of respiratory complexes2013

    • Author(s)
      Kotani T, Akabane S, Takeyasu K, Ueda T and Takeuchi N
    • Journal Title

      Nucleic Acids Research

      Volume: 41(6) Pages: 3713-22

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Nuclear Respiratory Factor 2beta (NRF-2beta) Recruits NRF-2alpha to the Nucleus by Binding to Importin-alpha:beta via an Unusual Monopartite-Type Nuclear Localization Signal2013

    • Author(s)
      Hayashi, R.
    • Journal Title

      Journal of molecular biology

      Volume: 425 Issue: 18 Pages: 3536-3548

    • DOI

      10.1016/j.jmb.2013.07.007

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Crystallization and preliminary X-ray analysis of peptidyl-tRNA hydrolase from Thermus thermophilus HB82013

    • Author(s)
      Matsumoto, A.
    • Journal Title

      Acta Crystallogr Sect F Struct Biol Cryst Commun

      Volume: 69 Issue: 3 Pages: 332-335

    • DOI

      10.1107/s1744309113003424

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of Chain Length of an Amphipathic Polypeptide Carrying the Repeated Amino Acid Sequence (LETLAKA)(n) on α-Helix and Fibrous Assembly Formation.2013

    • Author(s)
      Takei T., et al
    • Journal Title

      Biochemistry

      Volume: 52 Issue: 16 Pages: 2810-2820

    • DOI

      10.1021/bi400001c

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Unbiased tracking of the progression of mRNA and protein synthesis in bulk and in liposome-confined reactions2013

    • Author(s)
      van Nies, P
    • Journal Title

      Chembiochem

      Volume: 14 Issue: 15 Pages: 1963-1966

    • DOI

      10.1002/cbic.201300449

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Translation Enhancer Improves the Ribosome Liberation from Translation Initiation2013

    • Author(s)
      S. Takahashi, H. Furusawa, T. Ueda, and Y. Okahata
    • Journal Title

      J. Am. Chem. Soc.

      Volume: 135 Issue: 35 Pages: 13096-13106

    • DOI

      10.1021/ja405967h

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Direct monitoring of initiation factor dynamics through formation of 30S and 70S translation-initiation complexes on a quartz crystal microbalance2013

    • Author(s)
      Takahashi, S.
    • Journal Title

      Chemistry

      Volume: 19 Issue: 21 Pages: 6807-6816

    • DOI

      10.1002/chem.201203502

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Human G-proteins, ObgH1 and Mtg1, associate with the large mitochondrial ribosome subunit and are involved in translation and assembly of respiratory complexes2013

    • Author(s)
      Kotani, T.
    • Journal Title

      Nucleic acids research

      Volume: 2013 Issue: 6 Pages: 3713-3722

    • DOI

      10.1093/nar/gkt079

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structural basis for the substrate recognition and catalysis of peptidyl-tRNA hydrolase2012

    • Author(s)
      Ito K, Murakami R, Mochizuki M, Qi H, Shimizu, Y, Miura K, Ueda T, Uchiumi T
    • Journal Title

      Nucleic Acids Research

      Volume: 40(20) Pages: 10521-10531

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Crystal structure analysis of the translation factor RF3 (release factor 3)2012

    • Author(s)
      Kihira K, Shimizu Y, Shomura Y, Shibata N, Kitamura A, Ueda T, Ochi K, Higuchi Y
    • Journal Title

      Febs Letters

      Volume: 586(20) Pages: 3705-3709

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Robust in vitro affinity maturation strategy based on interface-focused high-throughput mutational scanning2012

    • Author(s)
      Fujino Y, Fujita R, Wada K, Fujishige K, Kanamori T, Hunt L, Shimizu Y, Ueda T
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 428(3) Pages: 395-400

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Target Specificity of an Autoreactive Pathogenic Human gamma delta-T Cell Receptor in Myositis2012

    • Author(s)
      Bruder J, Siewert K, Obermeier B, Malotka J, Scheinert P, Kellermann J, Ueda T, Hohlfeld R
    • Journal Title

      Journal of Biological Chemistry

      Volume: 287(25) Pages: 20986-20995

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Functional analysis of membranous F-0-a subunit of F1F0-ATP synthase by in vitro protein synthesis2012

    • Author(s)
      Kuruma Y, Suzuki T, Ono S, Yoshida M, Ueda T
    • Journal Title

      Biochemical Journal

      Volume: 442: Pages: 631-638

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Elongation Factor G Is a Critical Target during Oxidative Damage to the Translation System of Escherichia coli2012

    • Author(s)
      Nagano T, Kojima K, Hisabori T, Hayashi H, Morita E. H, Kanamori T,Miyagi T, Ueda T, Nishiyama Y
    • Journal Title

      Journal of Biological Chemistry

      Volume: 287 Pages: 28697-28704

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Global analysis of chaperone effects using a reconstituted cell-free translation system2012

    • Author(s)
      Niwa T, Kanamori T, Ueda T, Taguchi H
    • Journal Title

      Proceedings of the National Academy of Sciences of the United States of America

      Volume: 109(23) Pages: 8937-8942

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Traveling Time of a Translating Ribosome along Messenger RNA Monitored Directly on a Quartz Crystal Microbalance2012

    • Author(s)
      Takahashi S, Tsuji K, Ueda T, Okahata Y
    • Journal Title

      Journal of the American Chemical Society

      Volume: 134(15) Pages: 6793-6800

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Peptide screening using PURE ribosome display2012

    • Author(s)
      Ohashi H, Kanamori T, Osada E, Akbar BK, Ueda T
    • Journal Title

      Methods inmolecular biology

      Volume: 805 Pages: 251-259

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Peptide screening using PURE ribosome display2012

    • Author(s)
      Ohashi, H.,Kanamori, T. Osada, E., Atbar, B. K., Ueda, T.
    • Journal Title

      Methods in molecular biology

      Volume: 805 Pages: 251-259

    • DOI

      10.1007/978-1-61779-379-0_14

    • ISBN
      9781617793783, 9781617793790
    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Global Analysis of Chaperone Effects Using a Reconstituted Cell-Free Translation System2012

    • Author(s)
      Niwa, T., Kanamori T, Ueda, T.*, Taguchi, H.*
    • Journal Title

      Proc. Natl. Acad. Sci. U.S.A.

      Volume: 109 Issue: 23 Pages: 8937-8942

    • DOI

      10.1073/pnas.1201380109

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Functional analysis of membranous Fcra subunit of FIFo-ATP synthase by in vitro protein synthesis2012

    • Author(s)
      Kuruma, Y., Suzuki, T., Ono, S., Yoshida, M., Ueda, T.
    • Journal Title

      The Biochemical journal

      Volume: 442 Issue: 3 Pages: 631-638

    • DOI

      10.1042/bj20111284

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Creation of biosystems in a testtube2011

    • Author(s)
      Ueda T
    • Journal Title

      Genes & Genetic Systems

      Volume: 86(6) Pages: 395-395

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Recruitment of a species-specific translational arrest module to monitor different cellular processes2011

    • Author(s)
      Chiba S, Kanamori T, Ueda T, Akiyama Y, Pogliano K, Ito K
    • Journal Title

      Proceedings of the National Academy of Sciences of the United States of America

      Volume: 108(15) Pages: 6073-6078

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Crystallization and preliminary X-ray analysis of peptidyl-tRNA hydrolase from Escherichia coli in complex with the acceptor-TPsiC domain of tRNA2011

    • Author(s)
      Ito K, Qi H, Shimizu Y, Murakami R, Miura KI, Ueda T, Uchiumi T
    • Journal Title

      Acta crystallographica Section F, Structural biology and crystallization communications

      Volume: 67 (Pt 12) Pages: 1566-1569

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Single molecule imaging of the trans-translation entry process via anchoring of the tagged ribosome2011

    • Author(s)
      Zhou ZP, Shimizu Y, Tadakuma H, Taguchi H, Ito K, Ueda T
    • Journal Title

      Journal of biochemistry

      Volume: 149(5) Pages: 609-618

    • NAID

      10030585460

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Single molecule imaging of the trans-translation entry process via anch oring of the tagged ribosome.2011

    • Author(s)
      Zhou, Z-P., Shimizu, Y., Tadakuma, H.*, Taguchi, H., Ito, K., Ueda, T.
    • Journal Title

      J. Biochem.

      Volume: 149 Issue: 5 Pages: 609-618

    • DOI

      10.1093/jb/mvr010

    • NAID

      10030585460

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Recruitment of a species-specific arrest module to monitor different cellular processes2011

    • Author(s)
      Chiba, S., et al
    • Journal Title

      Proc.Natl.Acad.Sci, USA

      Volume: 108 Issue: 15 Pages: 6073-6078

    • DOI

      10.1073/pnas.1018343108

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] High-Resolution Thermodynamic Analysis of ABC-Transportation MSBA Reconstituted in Nanodiscs2014

    • Author(s)
      Takeaki Kawai, Jose Mannuel Martinez Caaveiro, Hisashi Tadakuma, Kouhei Tsumoto, Takuya Ueda
    • Organizer
      Biophysical Society 58th annual meeting Moscone Center
    • Place of Presentation
      San Francisco, USA
    • Year and Date
      2014-03-15
    • Related Report
      2013 Final Research Report
  • [Presentation] High-Resolution Thermodynamic Analysis of ABC-Transportation MSBA Reconstituted in Nanodiscs2014

    • Author(s)
      Takeaki Kawai, Jose Mannuel Martinez Caaveiro, Hisashi Tadakuma, Kouhei Tsumoto, Takuya Ueda
    • Organizer
      Biophysical Society 58th annual meeting
    • Place of Presentation
      Moscone Center, San Francisco, USA
    • Related Report
      2013 Annual Research Report
  • [Presentation] PURE systemの過去・現在・未来2013

    • Author(s)
      上田卓也
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸ポートピアホテル
    • Year and Date
      2013-12-04
    • Related Report
      2013 Final Research Report
  • [Presentation] The Pure System for Artificial Cells2013

    • Author(s)
      Takuya Ueda
    • Organizer
      International Astrobiology Workshop 2013
    • Place of Presentation
      ISAS Main Building 2F Conference Room, Sagamihara
    • Year and Date
      2013-11-28
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] 膜タンパク質合成システム‐Secトランスコロン‐を創る2013

    • Author(s)
      松林英明、車兪澈、上田卓也
    • Organizer
      細胞を創る研究会6.0
    • Place of Presentation
      山形大学鶴岡タウンキャンパス
    • Year and Date
      2013-11-14
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] Rational design of orthogonal gene neno-device on DNAorigami2013

    • Author(s)
      Takeya Masubuchi, Hisashi Tadakuma, Masayuki Endo, Yoshie Harada, Hiroshi Sugiyama, Takuya Ueda
    • Organizer
      第51回生物物理学会年会
    • Place of Presentation
      国立京都国際会館
    • Year and Date
      2013-10-28
    • Related Report
      2013 Final Research Report
  • [Presentation] 無細胞タンパク質合成系を用いたSecトランスコロンの構築2013

    • Author(s)
      松林英明、車兪澈、上田卓也
    • Organizer
      第8回無細胞生命科学研究会
    • Place of Presentation
      新潟大学中央図書館
    • Year and Date
      2013-10-21
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] 再構成型無細胞蛋白質合成系における30Sリボゾームサブユニットの再構成2013

    • Author(s)
      田丸大知、清水義宏、上田卓也
    • Organizer
      第8回無細胞生命科学研究会
    • Place of Presentation
      新潟大学中央図書館
    • Year and Date
      2013-10-21
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] in vitro Synthesis of Membrane Protein Machinery toward the Construction of Artificial Cell2013

    • Author(s)
      松林英明、車兪澈、上田卓也
    • Organizer
      12th European Conference on Artificial Life Taormina
    • Place of Presentation
      Italy
    • Year and Date
      2013-09-02
    • Related Report
      2013 Final Research Report
  • [Presentation] Autonomous Construction of Synthetic Cell Membrane2013

    • Author(s)
      車兪澈、上田卓也
    • Organizer
      12th European Conference on Artificial Life Taormina
    • Place of Presentation
      Italy
    • Year and Date
      2013-09-02
    • Related Report
      2013 Final Research Report
  • [Presentation] 無細胞翻訳系における30Sリボゾームサブユニットの再構成2013

    • Author(s)
      田丸大知、清水義宏、上田卓也
    • Organizer
      第15回日本 RNA学会年会
    • Place of Presentation
      愛媛県県民文化会館ひめぎんホール
    • Year and Date
      2013-07-24
    • Related Report
      2013 Final Research Report
  • [Presentation] PURE system の過去・現在・未来2013

    • Author(s)
      上田卓也
    • Organizer
      第36 回日本分子生物学会年会
    • Place of Presentation
      ポートピアホテル、神戸
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 無細胞翻訳系における30Sリボゾームサブユニットの再構成2013

    • Author(s)
      田丸大知、清水義宏、上田卓也
    • Organizer
      第15回日本RNA学会年会
    • Place of Presentation
      愛媛県県民文化会館ひめぎんホール
    • Related Report
      2013 Annual Research Report
  • [Presentation] in vitro Synthesis of Membrane Protein Machinery toward the Construction of Artificial Cell2013

    • Author(s)
      松林英明、車ゆうてつ、上田卓也
    • Organizer
      12th European Conference on Artificial Life
    • Place of Presentation
      Taormina, Italy
    • Related Report
      2013 Annual Research Report
  • [Presentation] Autonomous Constructiono of Synthetic Cell Membrane2013

    • Author(s)
      車ゆうてつ、上田卓也
    • Organizer
      12th European Conference on Artificial Life
    • Place of Presentation
      Taormina, Italy
    • Related Report
      2013 Annual Research Report
  • [Presentation] Dissection of Translation elongation in Yeast2013

    • Author(s)
      Hikari Hayashi, Satoshi Nishimura, Tetsuya Kotani, Syun Utsunomiya, Masanori Ishibashi, Takuya Ueda, Koichi Ito, Akira Kaji, Nono Tomita-Takeuchi
    • Organizer
      Ribo Club 2013 Annual Meeting
    • Place of Presentation
      Quebec, Canada
    • Related Report
      2013 Annual Research Report
  • [Presentation] Characterization of eukaryotic translation initiation factor 5A from Saccharomyces cerevisiae2013

    • Author(s)
      Satoshi Nishimura, Hikari Hayashi, Tetsuya Kotani, Takuya Ueda, Koichi Ito, Nono Tomita
    • Organizer
      Ribo Club 2013 Annual Meeting
    • Place of Presentation
      Quebec, Canada
    • Related Report
      2013 Annual Research Report
  • [Presentation] Rational design of orthogonal gene neno-device on DNA origami2013

    • Author(s)
      Takeya Masubuchi, Hisashi Tadakuma, Masayuki Endo, Yoshie Harada, Hiroshi Sugiyama, Takuya Ueda
    • Organizer
      第51回生物物理学会年会
    • Place of Presentation
      国立京都国際会館
    • Related Report
      2013 Annual Research Report
  • [Presentation] Engineered DNA glue and engineering protein in PUREsystem2013

    • Author(s)
      Ueda Takuya, Hao Qui
    • Organizer
      中国生物材料学会2013年会
    • Place of Presentation
      深セン市会議場, 中国
    • Related Report
      2013 Annual Research Report
  • [Presentation] Single moleculeimaging of the trans-translation entry process viaanchoring of the tagged ribosome2012

    • Author(s)
      Zhan-Ping Zhou, Yoshiiro shimizu, Hisashi Tadakuma, Hideki Taguchi, Koichi Ito, Takuya Ueda
    • Organizer
      日本生化学会第85回大会
    • Place of Presentation
      マリンメッセ福岡
    • Year and Date
      2012-12-15
    • Related Report
      2013 Final Research Report
  • [Presentation] Construction of Membren Protein Complexes by Cell-free System2012

    • Author(s)
      車兪澈、松林英明、鈴木俊治、吉田賢右、上田卓也
    • Organizer
      日本分子生物学会第35回年会
    • Year and Date
      2012-12-13
    • Related Report
      2013 Final Research Report
  • [Presentation] Construction of Membrane Protein Complexes by Cell-free System2012

    • Author(s)
      車兪澈、松林英明、鈴木俊治、吉田賢右、上田卓也
    • Organizer
      日本分子生物学会第35回年会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2012-12-13
    • Related Report
      2013 Final Research Report
  • [Presentation] DNAナノ構造体を用いたDNA-RNAポリメラーゼナノ複合体の構築と活性評価2012

    • Author(s)
      増渕岳也、多田隈尚史、遠藤政幸、杉山弘、原田慶恵、上田卓也
    • Organizer
      「細胞を作る」研究会5.0
    • Place of Presentation
      東京工業大学すずかけ台キャンパス
    • Year and Date
      2012-11-21
    • Related Report
      2013 Final Research Report
  • [Presentation] 無細胞系で創る膜タンパク室複合体2012

    • Author(s)
      車兪澈、松林英明、鈴木俊治、上田卓也
    • Organizer
      「細胞を作る」研究会5.0
    • Place of Presentation
      東京工業大学すずかけ台キャンパス
    • Year and Date
      2012-11-21
    • Related Report
      2013 Final Research Report
  • [Presentation] 無細胞タンパク質合成系による膜タンパク質複合体の構築2012

    • Author(s)
      車兪澈、松林英明、鈴木俊治、吉田賢右、上田卓也
    • Organizer
      第7回無細胞生命科学研究会
    • Place of Presentation
      愛媛大学南加記念ホール
    • Year and Date
      2012-11-18
    • Related Report
      2013 Final Research Report
  • [Presentation] The PURE system2012

    • Author(s)
      上田卓也
    • Organizer
      第10回無細胞科学松山国際シンポジウム
    • Place of Presentation
      松山全日空ホテル
    • Year and Date
      2012-09-25
    • Related Report
      2013 Final Research Report
  • [Presentation] The PURE system2012

    • Author(s)
      上田卓也
    • Organizer
      第10回無細胞科学松山国際シンポジウム
    • Place of Presentation
      松山全日空ホテル(松山市)(招待講演)
    • Year and Date
      2012-09-25
    • Related Report
      2011 Annual Research Report
  • [Presentation] Catalytic activity of MsbA reconstituted in nanodisc panodisc particles is modulated by remoteinteractions with the bilayer2012

    • Author(s)
      Takeaki Kawai, Jose Caaveiro, Toyomasa Katagiri, Hisashi Tadakuma, Takuya Ueda, Kouhei Tsumoto
    • Organizer
      第50回日本生物物理学会年会
    • Place of Presentation
      名古屋大学東山キャンパス
    • Year and Date
      2012-09-24
    • Related Report
      2013 Final Research Report
  • [Presentation] DNAナノ構造体を用いたDNA-RNAポリメラーゼナノ複合体の構築と活性評価2012

    • Author(s)
      増渕岳也、多田隈尚史、遠藤政幸、杉山弘、原田慶恵、上田卓也
    • Organizer
      第50回日本生物物理学会年会
    • Place of Presentation
      名古屋大学東山キャンパス
    • Year and Date
      2012-09-24
    • Related Report
      2013 Final Research Report
  • [Presentation] PURE systemでバイオシステムを作る2012

    • Author(s)
      上田卓也
    • Organizer
      日本農芸化学会2012年度大会
    • Place of Presentation
      京都女子大学
    • Year and Date
      2012-03-25
    • Related Report
      2013 Final Research Report
  • [Presentation] PURE system でバイオシステムを作る2012

    • Author(s)
      上田卓也
    • Organizer
      日本農芸化学会 2012年度大会
    • Place of Presentation
      京都女子大学(京都市)
    • Year and Date
      2012-03-25
    • Related Report
      2011 Annual Research Report
  • [Presentation] Catalytic activity of MsbA reconstituted in nanodisc particles is modulated by remote interactions with the bilayer2012

    • Author(s)
      河合 武揚
    • Organizer
      第50回日本生物物理学会年会
    • Place of Presentation
      名古屋大学東山キャンパス
    • Related Report
      2012 Annual Research Report
  • [Presentation] Single molecule imaging of the trans-translation entry process via anchoring of the tagged ribosome2012

    • Author(s)
      Zhan-Ping Zhou
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡国際会議場・マリンメッセ福岡
    • Related Report
      2012 Annual Research Report
  • [Presentation] 無細胞翻訳系を用いた機能性生体膜の構築2011

    • Author(s)
      車兪澈,松林英明,鈴木俊治,吉田賢右,上田卓也
    • Organizer
      第6回無細胞生命科学研究会
    • Place of Presentation
      兵庫県立大学姫路書写キャンパス
    • Year and Date
      2011-11-16
    • Related Report
      2013 Final Research Report
  • [Presentation] 無細胞翻訳系を用いた機能性生体膜の構築2011

    • Author(s)
      車兪激、松林英明、鈴木俊治、吉田賢右、上田卓也
    • Organizer
      第6回無細胞生命科学研究会
    • Place of Presentation
      兵庫県立大学(姫路市)
    • Year and Date
      2011-11-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] Construction of Synthetic BiomembranebyCell-Free Translation2011

    • Author(s)
      Yutetsu Kuruma, Hideaki Matsubayashi, Takuya Ueda
    • Organizer
      「細胞を創る」研究会4.0
    • Place of Presentation
      大阪千里ライフサイエンスセンター
    • Year and Date
      2011-10-28
    • Related Report
      2013 Final Research Report
  • [Presentation] The PURE system for Synthetic Biology2011

    • Author(s)
      Takuya Ueda
    • Organizer
      Synthesizing, Lifeand Biological Systems
    • Place of Presentation
      大阪千里ライフサイエンスセンター
    • Year and Date
      2011-10-26
    • Related Report
      2013 Final Research Report
  • [Presentation] The PURE system for Synthetic Biology2011

    • Author(s)
      Takuya Ueda
    • Organizer
      Synthesizing Life and Biological Systems
    • Place of Presentation
      千里ライフサイエンスセンター(大阪市)(招待講演)
    • Year and Date
      2011-10-26
    • Related Report
      2011 Annual Research Report
  • [Presentation] Construction of SecYEG translocon by cell-free protein synthesis system2011

    • Author(s)
      Hideaki Matsubayashi, Yutetsu Kuruma, Shin-ichiro M.Nomura, Ken-ichi Nishiyama, Takuya Ueda
    • Organizer
      Synthesizing Life and BiologicalSystems「細胞を創る」研究会4.0
    • Year and Date
      2011-10-25
    • Related Report
      2013 Final Research Report
  • [Presentation] Construction of SecYEG translocon by cell-free protein synthesis system2011

    • Author(s)
      Hideaki Matsubayashi, Yutetsu Kuruma, Shin-ichiro M.Nomura, Ken-ichi Nishiyama, Takuya Ueda
    • Organizer
      Synthesizing Life and Biological Systems,「細胞を創る」研究会4.0
    • Place of Presentation
      千里ライフサイエンスセンター(大阪市)
    • Year and Date
      2011-10-25
    • Related Report
      2011 Annual Research Report
  • [Presentation] 日本の大学における学生チームの編成と活動の実際2011

    • Author(s)
      上田卓也
    • Organizer
      第63回日本生物工学会大会
    • Place of Presentation
      東京農工大学(東京都)(招待講演)
    • Year and Date
      2011-09-26
    • Related Report
      2011 Annual Research Report
  • [Presentation] Construction of SecYEG translocon by cell-free protein synthesissystem2011

    • Author(s)
      Hideaki Matsubayashi, Yutetsu Kuruma, Shin-ichiro M. Nomura, Ken-ichi Nishiyama, Takuya Ueda
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      国立京都国際会館
    • Year and Date
      2011-09-24
    • Related Report
      2013 Final Research Report
  • [Presentation] Construction of SecYEG translocon by cell-free protein synthesis system2011

    • Author(s)
      Hideaki Matsubayashi, Yutetsu Kuruma, Shin-ichiro M. Nomura, Ken-ichi Nishiyama, Takuya Ueda
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      国立京都国際会館(京都市)
    • Year and Date
      2011-09-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] 試験管内でバイオシステムを作る2011

    • Author(s)
      上田卓也
    • Organizer
      日本遺伝学会第83回大会
    • Place of Presentation
      京都大学
    • Year and Date
      2011-09-20
    • Related Report
      2013 Final Research Report
  • [Presentation] 試験管内でバイオシステムを作る2011

    • Author(s)
      上田卓也
    • Organizer
      日本遺伝学会第83回大会
    • Place of Presentation
      京都大学(京都市)
    • Year and Date
      2011-09-20
    • Related Report
      2011 Annual Research Report
  • [Presentation] Constructing DNA-kinesin hybrid-nanomachine using theDNA-tile scaffold DNA-kinesin2011

    • Author(s)
      Yuya Miyazono, HisashiTadakuma, Takuya Ueda, Yoshie Harada
    • Organizer
      ハイブリッド・ナノマシンの構築日本生物物理学会
    • Place of Presentation
      兵庫県立大学
    • Year and Date
      2011-09-16
    • Related Report
      2013 Final Research Report
  • [Presentation] Artificial Kuruma, TosYoshida, Organelle hiharu Takuya for Suzuki, Ueda, Energy Production in Artificial Cell2011

    • Author(s)
      Yutetsu Masasuke
    • Organizer
      European Conference on Artificial Life 2011 Internationale Universitaire de Paris
    • Place of Presentation
      Paris France
    • Year and Date
      2011-08-08
    • Related Report
      2013 Final Research Report
  • [Presentation] Artificial Organelle for Energy Production in Artificial Cell2011

    • Author(s)
      Yutetsu Kuruma, Toshiharu Suzuki, Masasuke Yoshida, Takuya Ueda
    • Organizer
      European Conference on Artificial Life 2011
    • Place of Presentation
      The Cite Internationale Universitaire de Paris, Paris(France)
    • Year and Date
      2011-08-08
    • Related Report
      2011 Annual Research Report
  • [Presentation] Construction of SecYEG translocon by cell-free protein synthesis system2011

    • Author(s)
      Hideaki Matsubayashi, Yutetsu Kuruma, Shin-ichiro M. Nomura, Ken-ichi Nishiyama, Takuya Ueda
    • Organizer
      the Fifth International Meeting on Synthetic Biology Stanford University
    • Place of Presentation
      Stanford, California, USA
    • Year and Date
      2011-06-15
    • Related Report
      2013 Final Research Report
  • [Presentation] Construction of SecYEG translocon by cell-free protein synthesis system2011

    • Author(s)
      Hideaki Matsubayashi, Yutetsu Kuruma, Shin-ichiro M.Nomura, Ken-ichi Nishiyama, Takuya Ueda
    • Organizer
      the Fifth International Meeting on Synthetic Biology
    • Place of Presentation
      Stanford University, California (USA)
    • Year and Date
      2011-06-15
    • Related Report
      2011 Annual Research Report
  • [Book] 翻訳伸長制御のメカニズムとその意義, 実験医学 31(7)2013

    • Author(s)
      富田野乃、上田卓也
    • Related Report
      2013 Final Research Report
  • [Book] 合成生物学による翻訳系の新展開‐リボゾームや遺伝コードの改変を通して, 化学 68(6)2013

    • Author(s)
      多田隈尚史、上田卓也
    • Related Report
      2013 Final Research Report
  • [Book] Construction of the Synthetic Cell Based on the Reconstructed Cell-Free Translation System, PURE system, 化学とマイクロ・ナノシステム 10(2)2011

    • Author(s)
      車兪澈,上田卓也
    • Related Report
      2013 Final Research Report
  • [Remarks] 東京大学上田研究室業績

    • URL

      http://park.itc.u-tokyo.ac.jp/molbio/publications.html

    • Related Report
      2013 Annual Research Report

URL: 

Published: 2011-04-06   Modified: 2019-07-29  

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