Molecular Mechanism of Protein Maturation of Hydrogenase
Project/Area Number |
23247014
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Research Category |
Grant-in-Aid for Scientific Research (A)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Structural biochemistry
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Research Institution | Kyoto University |
Principal Investigator |
MIKI Kunio 京都大学, 理学(系)研究科(研究院), 教授 (10116105)
|
Co-Investigator(Renkei-kenkyūsha) |
ATOMI Haruyuki 京都大学, 大学院工学研究科, 教授 (90243047)
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Research Collaborator |
WATANABE Satoshi 京都大学, 大学院理学研究科, 特定研究員 (50432357)
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Project Period (FY) |
2011-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥47,060,000 (Direct Cost: ¥36,200,000、Indirect Cost: ¥10,860,000)
Fiscal Year 2013: ¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2012: ¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2011: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
|
Keywords | 成熟化タンパク質 / X線結晶構造解析 / 金属取り込み / Hypタンパク質 / 複合体構造 / X線結晶構造解析 / 構造生物学 |
Outline of Final Research Achievements |
Six Hyp proteins (HypA, B, C, D, E, and F) are primarily involved in the maturation process of [NiFe] hydrogenases which catalyze reversible hydrogen production and consumption. These Hyp proteins incorporate a complex Ni/Fe cluster into a protein subunit of [NiFe] hydrogenases. On the basis of our previous success in the structure determination of several Hyp proteins, we have investigated structure and function of transient complexes (HypAB, HypCD, and HypCDE complexes, and hydrogenase-large subunit (HyhL) and Hyp protein complexes) and reaction intermediates (carbamoylated and cyanated HypEs) to obtain insights into molecular mechanism of the [NiFe] hydrogenase maturation.
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Report
(4 results)
Research Products
(24 results)
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[Journal Article] Identification and Structure of a Novel Archaeal HypB for [NiFe] Hydrogenase Maturation2013
Author(s)
D. Sasaki, S. Watanabe, R. Matsumi, T. Shoji, A. Yasukochi, K. Tagashira, W. Fukuda, T. Kanai, H. Atomi, T. Imanaka, and K. Miki
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Journal Title
J. Mol. Biol.
Volume: 425
Issue: 10
Pages: 1627-1640
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Presentation] Identification and Structure of a Novel Archaeal HypB for [NiFe] Hydrogenase Maturation2012
Author(s)
D. Sasaki, S. Watanabe, R. Matsumi, T. Shoji, A. Yasukochi, K. Tagashira, W. Fukuda, T. Kanai, H. Atomi, T. Imanaka, and K. Miki
Organizer
27th European Crystallographic Meeting
Place of Presentation
Bergen, Norway
Related Report
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