| Project/Area Number |
23247014
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
MIKI Kunio 京都大学, 理学(系)研究科(研究院), 教授 (10116105)
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| Co-Investigator(Renkei-kenkyūsha) |
ATOMI Haruyuki 京都大学, 大学院工学研究科, 教授 (90243047)
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| Research Collaborator |
WATANABE Satoshi 京都大学, 大学院理学研究科, 特定研究員 (50432357)
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| Project Period (FY) |
2011-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥47,060,000 (Direct Cost: ¥36,200,000、Indirect Cost: ¥10,860,000)
Fiscal Year 2013: ¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2012: ¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2011: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
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| Keywords | 成熟化タンパク質 / X線結晶構造解析 / 金属取り込み / Hypタンパク質 / 複合体構造 / Ｘ線結晶構造解析 / 構造生物学 |
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| Outline of Final Research Achievements |
Six Hyp proteins (HypA, B, C, D, E, and F) are primarily involved in the maturation process of [NiFe] hydrogenases which catalyze reversible hydrogen production and consumption. These Hyp proteins incorporate a complex Ni/Fe cluster into a protein subunit of [NiFe] hydrogenases. On the basis of our previous success in the structure determination of several Hyp proteins, we have investigated structure and function of transient complexes (HypAB, HypCD, and HypCDE complexes, and hydrogenase-large subunit (HyhL) and Hyp protein complexes) and reaction intermediates (carbamoylated and cyanated HypEs) to obtain insights into molecular mechanism of the [NiFe] hydrogenase maturation.
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