|Budget Amount *help
¥48,230,000 (Direct Cost: ¥37,100,000、Indirect Cost: ¥11,130,000)
Fiscal Year 2014: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000)
Fiscal Year 2013: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000)
Fiscal Year 2012: ¥12,480,000 (Direct Cost: ¥9,600,000、Indirect Cost: ¥2,880,000)
Fiscal Year 2011: ¥12,740,000 (Direct Cost: ¥9,800,000、Indirect Cost: ¥2,940,000)
|Outline of Final Research Achievements
The dynamic structural change of the stator in the sodium-driven flagellar motor of Vibrio was analyzed for the mechanism of rotational energy conversion when the coupling ions flow in the stator of the motor. The ion uptake activity by the stator has been detected directly and firstly in vivo by atomic absorption analysis. In addition, although in Vibrio stator the mutation of the ion binding residues, PomB-D24N, give completely nonfunctional penotype, the additional PomA-N194D mutation suppress the defect. It is inferred that the ion binding capacity was recovered by the mutation. Furthermor, the structure of the C-terminal fragment of PomB (PomBC) was determined by crystallographic analysis and was compared with the structure of the correponding region of E. coli MotB. Based on these structural information chimeric proteins PotB made of PomB and MotB were constrected and carried out the functional analysis of these chimeric proteins.