| Budget Amount *help |
¥46,930,000 (Direct Cost: ¥36,100,000、Indirect Cost: ¥10,830,000)
Fiscal Year 2014: ¥14,170,000 (Direct Cost: ¥10,900,000、Indirect Cost: ¥3,270,000)
Fiscal Year 2013: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Fiscal Year 2012: ¥14,430,000 (Direct Cost: ¥11,100,000、Indirect Cost: ¥3,330,000)
Fiscal Year 2011: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
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| Outline of Final Research Achievements |
Protein-protein interactions are characterized by the extensive interaction surfaces with a number of the metastable states. The free energy landscapes of such complex interactions were investigated for the complex systems of Barnase/Barstar and EIN/HPr by the exhaustive sampling simulations of their all-atom model in explicit solvents. The simulation results revealed that the encounter complexes of the protein complex with micro molar Kd should be understood as the metastable states in broad equilibrium distributions, but not as those appeared in the kinetic process of the complex formation. Those metastable states make the functional native structure more transient, or entropically unstable.
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