| Budget Amount *help |
¥48,750,000 (Direct Cost: ¥37,500,000、Indirect Cost: ¥11,250,000)
Fiscal Year 2013: ¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000)
Fiscal Year 2012: ¥14,820,000 (Direct Cost: ¥11,400,000、Indirect Cost: ¥3,420,000)
Fiscal Year 2011: ¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000)
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| Outline of Final Research Achievements |
During recombination, homology search between two DNA molecules is a key reaction which is mediated by the RecA homolog Rad51 during mitosis. On the other hand, homology search in meiotic recombination is catalyzed by a collaborative action of two RecA homolog, Rad51 and Dmc1. Both Rad51 and Dmc1 form a filament on the single-stranded DNAs which is regulated in a positive and negative way. We identified a new protein complex containing Psy3, Csm2, Shu1 and Shu2 for Rad51 assembly. We determined a crystal structure of a core component of this complex, Psy3-Csm2. Surprisingly, Psy3-Csm2 shows a structural similarity to Rad51 dimer, although there is little sequence similarity between the proteins. Based on the structure, we propose how the Psy3-Csm2 promotes Rad51 filament on the DNA. This is very novel finding to understand molecular mechanism on how Rad51 mediators facilitate Rad51 filament formation.
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