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Analysis of mechanism involved in the assembly balance between centromere chromatin and/or heterochromatin and in higher cellular regulation.

Research Project

Project/Area Number 23247030
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Molecular biology
Research InstitutionKazusa DNA Research Institute

Principal Investigator

MASUMOTO Hiroshi  公益財団法人かずさDNA研究所, ヒトゲノム研究部, 室長 (70229384)

Co-Investigator(Renkei-kenkyūsha) NAKANO Megumi  (公益財団法人)かずさDNA 研究所, 研究員 (50542825)
OHZEKI Jun-ichirou  (公益財団法人)かずさDNA 研究所, 研究員 (30514088)
Project Period (FY) 2011-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥49,790,000 (Direct Cost: ¥38,300,000、Indirect Cost: ¥11,490,000)
Fiscal Year 2013: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
Fiscal Year 2012: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
Fiscal Year 2011: ¥28,990,000 (Direct Cost: ¥22,300,000、Indirect Cost: ¥6,690,000)
Keywords遺伝学 / 染色体 / ヘテロクロマチン / セントロメア / 人工染色体
Research Abstract

We investigated mechanism involved in the chromatin assembly balance between centromere chromatin and/or heterochromatin at human centromeric repetitive alphoid DNA. We found that tethering of histone acetyltransferases (HATs) to alphoid DNA arrays broke a cell type-specific barrier (heterochromatin formation) for de novo stable CENP-A chromatin assembly and induced assembly of stable Human Artificial Chromosomes (HACs) with the input alphoid DNA. We, then, tethered a huge variety of tetR-fusion proteins to the tetO-alphoid HAC and screened many positive and negative regulators for the CENP-A chromatin assembly using KAZUSA full-length cDNA library. We also developed a system targeting the chromatin assembly balance at endogenous centromeres by fusing these regulators and CENP-B.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (60 results)

All 2014 2013 2012 2011 Other

All Journal Article (34 results) (of which Peer Reviewed: 31 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (18 results) (of which Invited: 5 results) Book (1 results) Remarks (4 results) Patent(Industrial Property Rights) (3 results) (of which Overseas: 3 results)

  • [Journal Article] The epigenetic regulator Uhrf1 facilitates the proliferation and maturation of colonic regulatory T cells2014

    • Author(s)
      Obata Y, Furusawa Y, Endo TA, Sharif J, Takahashi D, Atarashi K, Nakayama M, Onawa S, Fujimura Y, Takahashi M, Ikawa T, Otsubo T, Kawamura YI, Dohi T, Tajima S, Masumoto H, Ohara O, Honda K, Hori S, Ohno H, Koseki H, Hase K
    • Journal Title

      Nat Immunol. Apr 28.

      Volume: 15 Pages: 571-579

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Human artificial chromosome based gene delivery vectors for biomedicine and biotechnology2014

    • Author(s)
      Kouprina N, Tomilin AN, Masumoto H, Earnshaw WC, and Larionov V
    • Journal Title

      Expert Opin. Drug Deliv. 総説

      Volume: 11(4) Pages: 517-35

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] The epigenetic regulator Uhrf1 facilitates proliferation and maturation of colonic regulatory T cells2014

    • Author(s)
      Yuuki Obata*, Yukihiro Furusawa*, Takaho A. Endo, Jafar Sharif, Daisuke Takahashi, Koji Atarashi, Manabu Nakayama, Satoshi Onawa, Yumiko Fujimura, Masumi Takahashi, Tomokatsu Ikawa, Takeshi Otsubo, Yuki I. Kawamura, Taeko Dohi, Shoji Tajima, Osamu Ohara, Kenya Honda, Shohei Hori, Hiroshi Ohno, Haruhiko Koseki and Koji Hase. 【* : 筆頭筆者】
    • Journal Title

      Nature Immunology

      Volume: 15 Issue: 6 Pages: 571-579

    • DOI

      10.1038/ni.2886

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Human artificial chromosome based gene delivery vectors for biomedicine and biotechnology.2014

    • Author(s)
      Kouprina N, Tomilin AN, Masumoto H, Earnshaw WC, and Larionov V
    • Journal Title

      Expert Opin. Drug Deliv.

      Volume: 11(4) Issue: 4 Pages: 517-535

    • DOI

      10.1517/17425247.2014.882314

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Identification of Novel α-N-Methylation Modification of CENP-B That Regulates Its Binding to the Centromeric DNA2013

    • Author(s)
      Dai X, Otake K, You C, Cai Q, Wang Z, Masumoto H and Wang Y
    • Journal Title

      J Proteome Res. Sep 6

      Volume: 12(9) Pages: 4167-75

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] A new assay for measuring chromosome instability (CIN) and identification of drugs that elevate CIN in cancer cells2013

    • Author(s)
      Lee H-S, Lee NC, Grimes BR, Samoshkin A, Kononenko AV, Bansal R, Masumoto H, Earnshaw WC, Kouprina N, Larionov V
    • Journal Title

      BioMed Central Cancer, May 22

      Volume: 13(1) Pages: 252-252

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Protecting a transgene expression from the HAC-based vector by different chromatin insulators2013

    • Author(s)
      Lee NC, Kononenko AV, Lee H-S, Tolkunova EN, Liskovykh MA, Masumoto H, Earnshaw WC, Tomilin AN, Larionov V, Kouprina N
    • Journal Title

      Cell Mol Life Sci., Oct

      Volume: 70(19) Pages: 3723-37

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Nap1 regulates proper CENP-B binding to nucleosomes2013

    • Author(s)
      Tachiwana H, Miya Y, Shono N, Ohzeki J, Osakabe A, Otake K, Larionov V, Earnshaw WC, Masumoto H and Kurumizaka H
    • Journal Title

      Nucleic. Acid. Res.

      Volume: 41(5) Pages: 2869-2880

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Esperanto for histones : CENP-A, not CenH3, is the centromeric histone H3 variant2013

    • Author(s)
      Earnshaw WC, Allshire RC, Black BE, Bloom K, Brinkley BR, Brown W, Cheeseman IM, Choo KHA, Copenhaver GP, DeLuca JG, Desai A, Diekmann S, Erhardt S, Fitzgerald-Hayes M, Foltz D, Fukagawa T, Gassmann R, Gerlich DW, Glover DM, Gorbsky GJ, Harrison SC, Heun P, Hirota T, Jansen LET, Karpen G, Kops GJPL, Lampson MA, Lens SM, Losada A, Luger K, Maiato H, Maddox PS, Margolis RM, Masumoto H, McAinsh AD, Mellone BG, Meraldi P, Musacchio A, Oegema K, O'Neill RJ, Salmon ED, Scott KC, Straight AF, Stukenberg PT, Sullivan BA, Sullivan KF, Sunkel CE, Swedlow JR, Walczak CE, Warburton PE, Westermann S, Willard HF, Wordeman L, Yanagida M, Yen TJ, Yoda K, Cleveland DW
    • Journal Title

      Chromosome Res. 総説

      Volume: 21(2) Pages: 101-106

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] A new generation of human artificial chromosomes for functional genomics and gene therapy2013

    • Author(s)
      Kouprina N, Earnshaw WC, Masumoto H, and Larionov V
    • Journal Title

      Cell. Mol. Life Sci. 総説

      Volume: 70(7) Pages: 1135-48

    • Related Report
      2013 Final Research Report
  • [Journal Article] Identification of Novel α-N-Methylation Modification of CENP-B That Regulates Its Binding to the Centromeric DNA.2013

    • Author(s)
      Dai X, Otake K, You C, Cai Q, Wang Z, Masumoto H and Wang Y
    • Journal Title

      J. Proteome Res.

      Volume: 12(9) Issue: 9 Pages: 4167-4175

    • DOI

      10.1021/pr400498y

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A new assay for measuring chromosome instability (CIN) and identification of drugs that elevate CIN in cancer cells.2013

    • Author(s)
      Lee H-S, Lee NC, Grimes BR, Samoshkin A, Kononenko AV, Bansal R, Masumoto H, Earnshaw WC, Kouprina N, Larionov V
    • Journal Title

      BioMed Central Cancer

      Volume: 13(1) Issue: 1 Pages: 252-252

    • DOI

      10.1186/1471-2407-13-252

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Protecting a transgene expression from the HAC-based vector by different chromatin insulators.2013

    • Author(s)
      Lee NC, Kononenko AV, Lee H-S, Tolkunova EN, Liskovykh MA, Masumoto H, Earnshaw WC, Tomilin AN, Larionov V, Kouprina N
    • Journal Title

      Cell Mol Life Sci.

      Volume: 70(19) Issue: 19 Pages: 3723-3737

    • DOI

      10.1007/s00018-013-1362-9

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nap1 regulates proper CENP-B binding to nucleosomes2013

    • Author(s)
      Tachiwana H, Miya Y, Shono N, Ohzeki J, Osakabe A, Otake K, Larionov V, Earnshaw WC, Kimura H, Masumoto H, Kurumizaka H
    • Journal Title

      Nucleic Acids Res

      Volume: 5 Issue: 5 Pages: 2869-2880

    • DOI

      10.1093/nar/gks1464

    • Related Report
      2013 Annual Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A new generation of human artificial chromosomes for functional genomics and gene therapy.2013

    • Author(s)
      Kouprina N, Earnshaw WC, Masumoto H, and Larionov V
    • Journal Title

      Cell. Mol. Life Sci.

      Volume: 70(7) Issue: 7 Pages: 1135-1148

    • DOI

      10.1007/s00018-012-1113-3

    • Related Report
      2013 Annual Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Esperanto for histones: CENP-A, not CenH3, is the centromeric histone H3 variant.2013

    • Author(s)
      Earnshaw WC, Allshire RC, Black BE, Bloom K, Brinkley BR, Brown W, Cheeseman IM, Choo KHA, Masumoto H, Cleveland DW et al
    • Journal Title

      Chromosome Res.

      Volume: 21(2) Issue: 2 Pages: 101-106

    • DOI

      10.1007/s10577-013-9347-y

    • Related Report
      2013 Annual Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Centromere Architecture Breakdown Induced by the Viral E3 Ubiquitin Ligase ICP0 Protein of Herpes Simplex Virus Type 12012

    • Author(s)
      Gross S, Catez F, Masumoto H and Lomonte P
    • Journal Title

      PLOS ONE

      Volume: 7(9)

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Organization of Synthetic Alphoid DNA Array in Human Artifcial Chromosome (HAC) with a Conditional Centromere2012

    • Author(s)
      Kouprina N, Samoshkin A, Erliandri I, Nakano M, Lee H-S, Fu H, Iida Y, Aladjem M, Oshimura M, Masumoto H, Earnshaw WC and Larionov V
    • Journal Title

      ACS Synth.Biol.

      Volume: 1(12) Pages: 590-601

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] HACking the centromere chromatin code : insights from human artificial chromosomes2012

    • Author(s)
      Bergmann JH, Martins NM, Larionov V, Masumoto H, Earnshaw WC
    • Journal Title

      Chromosome Res.

      Volume: 20(5) Pages: 505-519

    • Related Report
      2013 Final Research Report
  • [Journal Article] Breaking the HAC Barrier : Histone H3K9 acetyl/methyl balance regulates CENP-A assembly2012

    • Author(s)
      Ohzeki J, Bergmann JH, Kouprina N, Noskov V, Nakano M, Kimura H, Earnshaw WC, Larionov V and Masumoto H
    • Journal Title

      EMBO J

      Volume: 31(10) Pages: 2391-2402

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Epigenetic Engineering : Histone H3K9 acetylation is compatible with kinetochore structure and function2012

    • Author(s)
      Bergmann JH, Jakubsche J, Martins NM, Nakano M, Kimura H, Kelly DA, Turner BM, Masumoto H, Larionov V and Earnshaw WC
    • Journal Title

      J. Cell Science

      Volume: 125(Pt 2) Pages: 411-421

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Centromere Architecture Breakdown Induced by the Viral E3 Ubiquitin Ligase ICP0 Protein of Herpes Simplex Virus Type 1.2012

    • Author(s)
      Gross S.
    • Journal Title

      PLOS ONE

      Volume: 7(9) Issue: 9 Pages: e44227-e44227

    • DOI

      10.1371/journal.pone.0044227

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Breaking the HAC Barrier : Histone H3K9 acetyl/ methyl balance regulates CENP-A assembly2012

    • Author(s)
      Ohzeki J-i, Bergmann JH, Kouprina N, Noskov VN, Nakano M, Kimura H, Earnshaw WC, Larionov V, and Masumoto H
    • Journal Title

      EMBO J

      Volume: (in press) Issue: 10 Pages: 2391-2402

    • DOI

      10.1038/emboj.2012.82

    • Related Report
      2012 Annual Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Epigenetic Engineering : Histone H3K9 acetylation is compatible with kinetochore structure and function2012

    • Author(s)
      Bergmann JH, Jakubsche JN, Martins NM, Kagansky A, Nakano M, Kimura H, Kelly DA, Turner BM, Masumoto H, Larionov V, and Earnshaw WC
    • Journal Title

      J Cell Sci

      Volume: 125 Issue: 2 Pages: 411-421

    • DOI

      10.1242/jcs.090639

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Organization of Synthetic Alphoid DNA Array in Human Artificial Chromosome (HAC) with a Conditional Centromere.2012

    • Author(s)
      Kouprina N.
    • Journal Title

      ACS Synth.Biol.

      Volume: 1(12) Pages: 590-601

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] HACking the centromere chromatin code: insights from human artificial chromosomes.2012

    • Author(s)
      Bergmann J.H.
    • Journal Title

      Chromosome Res.

      Volume: 20(5) Issue: 5 Pages: 505-519

    • DOI

      10.1007/s10577-012-9293-0

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Epigenetic Engineering : Histone H3K9 acetylation is compatible with kinetochore structure and function2012

    • Author(s)
      Jan H.Bergmann
    • Journal Title

      J.Cell Science

      Volume: 15;125(Pt 2) Pages: 411-421

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] HP1γ links histone methylation marks to meiotic synapsis in mice2011

    • Author(s)
      Takada Y, Naruse C, Costa Y, Shirakawa T, Tachibana M, Sharif J, Kezuka-Shiotani F, Kakiuchi D, Masumoto H, Shinkai Y, Ohbo K, Peters AH, Turner JM, Asano M, Koseki
    • Journal Title

      Development.

      Volume: 138 Pages: 4207-17

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Human Artificial Chromosome (HAC) vector with a conditional centromere for correction of genetic deficiencies in human cells2011

    • Author(s)
      Kim J-H, Kononenko A, Erliandri I, Kim T, Nakano M, Iida Y, Barrett CJ, Oshimura M, Masumoto H, Earnshaw WC, Larionov V & Kouprina N
    • Journal Title

      PNAS

      Volume: 108 Pages: 20048-53

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Epigenetic engineering shows H3K4me2 is required for HJURP targeting and CENP-A assembly on a synthetic human kinetochore2011

    • Author(s)
      Bergmann JH, Rodriguez MG, Martins NMC, Kimura H, Kelly DA, Masumoto H, Larionov V, Jansen LET and Earnshaw WC
    • Journal Title

      EMBO J.

      Volume: 30 Pages: 328-340

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] HP1γ links histone methylation marks to meiotic synapsis in mice2011

    • Author(s)
      Y.Takada
    • Journal Title

      Development

      Volume: 138 Pages: 4207-4217

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Human Artificial Chromosome (HAC) vector with a conditional centromere for correction of genetic deficiencies in human cells2011

    • Author(s)
      Jung-Hyun Kim
    • Journal Title

      PNAS

      Volume: 108 Issue: 50 Pages: 20048-20053

    • DOI

      10.1073/pnas.1114483108

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Epigenetic engineering shows H3K4me2 is required for HJURP targeting and CENP-A assembly on a synthetic human kinetochore2011

    • Author(s)
      Bergmann JH, Rodriguez MG, Martins NM, Kimura H, Kelly DA, Masumoto H, Larionov V, Jansen LE, and Earnshaw WC
    • Journal Title

      EMBO J

      Volume: 30 Issue: 2 Pages: 328-340

    • DOI

      10.1038/emboj.2010.329

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] セントロメア形成を決定するクロマチン集合バランス2011

    • Author(s)
      舛本寛
    • Journal Title

      生体の科学

      Volume: 60(5) Pages: 458-459

    • Related Report
      2011 Annual Research Report
  • [Presentation] ヒト人工染色体を用いたセントロメアとヘテロクロマチンの集合機構の解析2014

    • Author(s)
      舛本寛
    • Organizer
      高等研プロジェクト第1回「クロマチン・デコーディング」研究会
    • Place of Presentation
      国際高等研
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] セントロメア形成を制御するアセチル化酵素の探索2013

    • Author(s)
      大関淳一郎、庄野暢晃、大竹興一郎、中野めぐみ、William C. Earnshaw、Vladimir Larionov、長瀬隆弘、舛本 寛
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド
    • Related Report
      2013 Annual Research Report
  • [Presentation] 脱落制御可能なヒト人工染色体(HAC) を用いたiPS 細胞の作製と細胞選抜への利用2013

    • Author(s)
      岡村 佳明、霜島 司、中野めぐみ、大関 淳一郎、長谷川 嘉則、 池野 正史、William C. Earnshaw, Vladimir Larionov、 舛本 寛
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド
    • Related Report
      2013 Annual Research Report
  • [Presentation] 細胞へ導入されたDNAの運命 : 人工染色体形成、宿主染色体への組込み、核からの脱落、はどのように起こるか?2012

    • Author(s)
      舛本寛、大関淳一郎、中野めぐみ、Larionov V and Earnshaw WC
    • Organizer
      第84回日本遺伝学会大会
    • Place of Presentation
      博多
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Exploring of centromere acetylating mechanism using tetO-alphoid system2012

    • Author(s)
      Ohzeki J, Shono N, Otake K, Nakano M, Earnshaw WC, Larionov V, Nagase T, Masumoto H
    • Organizer
      第30回染色体ワークショップ、第11回核ダイナミクス研究会
    • Place of Presentation
      淡路夢舞台国際会議(口頭発表)
    • Related Report
      2013 Final Research Report
  • [Presentation] Analysis of centromere chromatin assembly using tetO/tetR synthetic DNA system2012

    • Author(s)
      Shono N, Ohzeki J, Nakano M, Nagase T, Earnshaw WC, Larionov V, Masumoto H
    • Organizer
      第35回日本分子生物学会
    • Place of Presentation
      福岡国際会議場(口頭発表)
    • Related Report
      2013 Final Research Report
  • [Presentation] Heterochromatin assembly balance determines the fate of de novo kinetochre formation on satellite DNA(ミニシンポジウム主催)2012

    • Author(s)
      Masumoto H, Ohzeki J, Nakano M, Vladimir N, Kouprina N, Earnshaw WC and Larionov V
    • Organizer
      第63回日本細胞生物学会大会
    • Place of Presentation
      札幌
    • Related Report
      2013 Final Research Report
  • [Presentation] 細胞へ導入されたDNAの運命:人工染色体形成、宿主染色体への組込み、核からの脱落、はどのように起こるか?2012

    • Author(s)
      舛本寛
    • Organizer
      第84回日本遺伝学会大会
    • Place of Presentation
      九州大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] Exploring of centromere acetylating mechanism using tetO-alphoid system.2012

    • Author(s)
      Ohzeki J.
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2012 Annual Research Report
  • [Presentation] The Analysis of regulation and maintenance mechanisms of centromere functional structure with chromatin-alterable human artificial chromosome system.2012

    • Author(s)
      Nakano M.
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2012 Annual Research Report
  • [Presentation] The effect of CENP-B binding to Alphoid DNA on the formation and maintenance of CENP-A chromatin.2012

    • Author(s)
      Otake K.
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2012 Annual Research Report
  • [Presentation] Analysis of centromere chromatin assembly using tetO/tetR synthetic DNA system.2012

    • Author(s)
      Shono N.
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2012 Annual Research Report
  • [Presentation] ヒト人工染色体を創って調べる2011

    • Author(s)
      舛本寛
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都
    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Invited
  • [Presentation] Chromatin assembly balance determines the fate of de novo kinetochre formation on satellite DNA2011

    • Author(s)
      Masumoto H, Ohzeki J, Nakano M, Kouprina N, Larionov V, Earnshaw WC
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] セントロメアをアセチル化する機構の探索2011

    • Author(s)
      大関淳一郎、庄野暢晃、中野めぐみ、 William C. Earnshaw, Vladimir Larionov、長瀬隆弘、舛本寛
    • Organizer
      第29回染色体ワークショップ
    • Place of Presentation
      仙台(口頭)
    • Related Report
      2013 Final Research Report
  • [Presentation] A chromatin assembly balance on satellite DNA determines the fate of de novo kinetochore and HAC formation2011

    • Author(s)
      Masumoto H, Ohzeki J, Nakano M, Kouprina N, Larionov V and Earnshaw WC
    • Organizer
      Japanese-German JSPS and DFG-funded workshop "Centromeres and Artificial Chromosomes"
    • Place of Presentation
      Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Heterochromatin assembly balance determines the fate of de novo kinetochre formation on satellite DNA2011

    • Author(s)
      Hiroshi Masumoto
    • Organizer
      第63回日本細胞生物学会大会
    • Place of Presentation
      札幌
    • Related Report
      2011 Annual Research Report
  • [Presentation] Chromatmin assembly balance determines the fate of de novo kinetochre formation on satellite DNA2011

    • Author(s)
      Hiroshi Masumoto
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2011 Annual Research Report
  • [Book] セントロメア形成を決定するクロマチン集合バランス, 生体の科62(5)2011

    • Author(s)
      舛本寛
    • Related Report
      2013 Final Research Report
  • [Remarks]

    • URL

      http://www.kazusa.or.jp/j/laboratories/labo_cellengineering.html

    • Related Report
      2013 Final Research Report
  • [Remarks] かずさDNA研究所 研究内容>細胞工学研究室

    • URL

      http://www.kazusa.or.jp/j/laboratories/organization.html

    • Related Report
      2013 Annual Research Report
  • [Remarks] かずさDNA研究所 研究内容>細胞工学研究室

    • URL

      http://www.kazusa.or.jp/j/laboratories/lab_cellengineering.html

    • Related Report
      2012 Annual Research Report
  • [Remarks] 更新作業中

    • URL

      http://www.kazusa.or.jp/j/laboratories/laboratories.html

    • Related Report
      2011 Annual Research Report
  • [Patent(Industrial Property Rights)] A method for positively or negatively regulating the assembly of newly synthesized CENP-A to exogenous alphoid DNA containing CENP-B boxes in mammalian cell lines2012

    • Inventor(s)
      Masumoto H, Ohzeki J, Larionov V, Earnshaw WC
    • Industrial Property Rights Holder
      かずさDNA研究所
    • Industrial Property Rights Type
      特許
    • Filing Date
      2012-11-22
    • Related Report
      2013 Final Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] A method for CENP-A assembly2012

    • Inventor(s)
      Masumoto H.
    • Industrial Property Rights Holder
      Masumoto H.
    • Industrial Property Rights Type
      特許
    • Filing Date
      2012-11-22
    • Related Report
      2012 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] A method for positively or negatively regulating the assembly of newly synthesized CENP-A to exogenous alphoid DNA containing CENP-B boxes in mammalian cell lines2011

    • Inventor(s)
      H.Masumoto, J.Ohzeki, V.Larionov, W.C.Earnshaw
    • Industrial Property Rights Holder
      かずさDNA研究所
    • Filing Date
      2011-11-22
    • Related Report
      2011 Annual Research Report
    • Overseas

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Published: 2011-04-06   Modified: 2019-07-29  

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