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Elucidation of new functions of intermediate filaments linking a relationship between proliferation and differentiation

Research Project

Project/Area Number 23247035
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Cell biology
Research InstitutionAichi Cancer Center Research Institute

Principal Investigator

INAGAKI Masaki  愛知県がんセンター(研究所), 腫瘍医化学部, 部長 (30183007)

Co-Investigator(Kenkyū-buntansha) IZAWA Ichiro  愛知県がんセンター(研究所), 腫瘍医化学部, 室長 (20311441)
GOTO Hidemasa  愛知県がんセンター(研究所), 腫瘍医化学部, 室長 (20393126)
KASAHARA Kousuke  愛知県がんセンター(研究所), 腫瘍医化学部, 研究員 (90455535)
Project Period (FY) 2011-11-18 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥29,380,000 (Direct Cost: ¥22,600,000、Indirect Cost: ¥6,780,000)
Fiscal Year 2013: ¥14,690,000 (Direct Cost: ¥11,300,000、Indirect Cost: ¥3,390,000)
Fiscal Year 2012: ¥14,690,000 (Direct Cost: ¥11,300,000、Indirect Cost: ¥3,390,000)
Keywords中間径フィラメント / 増殖 / 分化 / ケラチン / 細胞骨格 / 中心体
Research Abstract

To uncover roles of intermediate filaments in proliferation and differentiation, we studied the functions of trichoplein, a keratin-binding protein, and trichoplein-related proteins. We found that trichoplein-Aurora-A pathway is required for G1 progression through a key role in the continuous suppression of primary cilia assembly. On the other hand, we revealed that knock-in mice expressing phosphodeficient vimentin variants developed binucleation and aneuploidy in lens epithelial cells, which promoted lens cataract, an aging phenotype.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (41 results)

All 2014 2013 2012 2011 Other

All Journal Article (22 results) (of which Peer Reviewed: 11 results,  Open Access: 3 results) Presentation (12 results) (of which Invited: 1 results) Book (3 results) Remarks (4 results)

  • [Journal Article] New insights into roles of intermediate filament phosphorylation and progeria pathogenesis2014

    • Author(s)
      Goto, H., and Inagaki, M.
    • Journal Title

      IUBMB Life

      Volume: 66 Pages: 195-200

    • Related Report
      2013 Final Research Report
  • [Journal Article] Cdk1 Coordinates Timely Activation of MKlp2 Kinesin with Relocation of the Chromosome Passenger Complex for Cytokinesis2014

    • Author(s)
      Kitagawa, M., Fung, S.Y.S., Hameed, U.F.S., Goto, H., Inagaki, M., Lee, S.H.
    • Journal Title

      Cell Rep.

      Volume: 7 Pages: 166-179

    • Related Report
      2013 Final Research Report
  • [Journal Article] Inhibition of endocytic vesicle fusion by Plk1-mediated phosphorylation of vimentin during mitosis2014

    • Author(s)
      Ikawa, K., Satou, A., Fukuhara, M., Matsumura, S., Sugiyama, N., Goto, H., Fukuda, M., Inagaki, M., Ishihama, Y. and Toyoshima, F.
    • Journal Title

      Cell Cycle

      Volume: 13 Pages: 126-137

    • Related Report
      2013 Final Research Report
  • [Journal Article] Inhibition of endocytic vesicle fusion by Plk1-mediated phosphorylation of vimentin during mitosis.2014

    • Author(s)
      Ikawa, K.
    • Journal Title

      Cell Cycle

      Volume: 13 Issue: 1 Pages: 126-137

    • DOI

      10.4161/cc.26866

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cdk1 Coordinates Timely Activation of MKlp2 Kinesin with Relocation of the Chromosome Passenger Complex for Cytokinesis.2014

    • Author(s)
      Kitagawa, M.
    • Journal Title

      Cell Rep.

      Volume: 7 Issue: 1 Pages: 166-179

    • DOI

      10.1016/j.celrep.2014.02.034

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] New Insights into Roles of Intermediate Filament Phosphorylation and Progeria Pathogenesis.2014

    • Author(s)
      Goto, H.
    • Journal Title

      IUBMB Life

      Volume: 66 Issue: 3 Pages: 195-200

    • DOI

      10.1002/iub.1260

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Defect of mitotic vimentin phosphorylation causes microophthalmia and cataract via aneuploidy and senescence in lens epithelial cells2013

    • Author(s)
      Matsuyama, M., Tanaka, H., Inoko, A., Goto, H., Yonemura, S., Kobori, K., Hayashi, Y., Kondo, E., Itohara, S., Izawa, I. and Inagaki, M.
    • Journal Title

      J. Biol. Chem.

      Volume: 288 Pages: 35626-35635

    • Related Report
      2013 Final Research Report
  • [Journal Article] PI 3-kinase-dependent phosphorylation of Plk1-Ser99 promotes association with 14-3-3γ and is required for metaphase-anaphase transition2013

    • Author(s)
      Kasahara, K., Goto, H., Izawa, I., Kiyono, T., Watanabe, N., Elowe, S., Nigg, E.A. and Inagaki, M.
    • Journal Title

      Nat. Commun.

      Volume: 4 Issue: 1 Pages: 1882-1882

    • DOI

      10.1038/ncomms2879

    • Related Report
      2013 Annual Research Report 2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Cell cycle progression by the repression of primary cilia formation in proliferating cells2013

    • Author(s)
      Goto, H., Inoko, A. and Inagaki, M.
    • Journal Title

      Cell. Mol. Life Sci.

      Volume: 70 Pages: 3893-3905

    • Related Report
      2013 Final Research Report
  • [Journal Article] The p90 ribosomal S6 kinase (RSK) inhibitor BI-D1870 prevents gamma irradiation-induced apoptosis and mediates senescence via RSK-and p53-independent accumulation of p21WAF1/CIP12013

    • Author(s)
      Neise, D., Sohn, D., Stefanski, A., Goto, H., Inagaki, M., Wesselborg, S., Budach, W., Stuhler, K. and Janicke, R.U.
    • Journal Title

      Cell Death and Dis.

      Volume: 4

    • Related Report
      2013 Final Research Report
  • [Journal Article] Keratin 8 Is Required for the Maintenance of Architectural Structure in Thymus Epithelium2013

    • Author(s)
      Odaka, C., Loranger, A., Takizawa, K., Ouellet, M., Tremblay, M.J., Murata, S., Inoko, A., Inagaki, M. and Marceau, N.
    • Journal Title

      PLoS ONE

      Volume: 8

    • Related Report
      2013 Final Research Report
  • [Journal Article] Detection of vimentin serine phosphorylation by multicolor Quenchbodies2013

    • Author(s)
      Jeong, H.J., Ohmuro-Matsuyama, Y., Ohashi, H., Ohsawa, F., Tatsu, Y., Inagaki, M. and Ueda, H.
    • Journal Title

      Biosens Bioelectron

      Volume: 40 Pages: 17-23

    • Related Report
      2013 Final Research Report
  • [Journal Article] Keratin 8 Is Required for the Maintenance of Architectural Structure in Thymus Epithelium.2013

    • Author(s)
      Odaka, C.
    • Journal Title

      PLoS ONE

      Volume: 8 Issue: 9 Pages: 75101-75101

    • DOI

      10.1371/journal.pone.0075101

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The p90 ribosomal S6 kinase (RSK) inhibitor BI-D1870 prevents gamma irradiation-induced apoptosis and mediates senescence via RSK- and p53-independent accumulation of p21WAF1/CIP1.2013

    • Author(s)
      Neise, D.
    • Journal Title

      Cell Death Dis.

      Volume: 4 Issue: 10 Pages: e859-e859

    • DOI

      10.1038/cddis.2013.386

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Defect of mitotic vimentin phosphorylation causes microophthalmia and cataract via aneuploidy and senescence in lens epithelial cells.2013

    • Author(s)
      Matsuyama, M.
    • Journal Title

      J. Biol. Chem.

      Volume: 288 Issue: 50 Pages: 35626-35635

    • DOI

      10.1074/jbc.m113.514737

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cell cycle progression by the repression of primary cilia formation in proliferating cells.2013

    • Author(s)
      Goto, H.
    • Journal Title

      Cell. Mol. Life Sci.

      Volume: 70 Issue: 20 Pages: 3893-3905

    • DOI

      10.1007/s00018-013-1302-8

    • Related Report
      2013 Annual Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Trichoplein and Aurora A block aberrant primary cilia assembly in proliferating cells2012

    • Author(s)
      Inoko, A., Matsuyama, M., Goto, H., Ohmuro-Matsuyama, Y., Hayashi, Y., Enomoto, M., Ibi, M., Urano, T., Yonemura, S., Kiyono, T., Izawa, I. and Inagaki, M.
    • Journal Title

      J. Cell Biol.

      Volume: 197 Pages: 391-405

    • Related Report
      2013 Final Research Report
  • [Journal Article] P90 RSK arranges Chk1 in the nucleus for monitoring of genomic integrity during cell proliferation2012

    • Author(s)
      Li, P., Goto, H., Kasahara, K., Matsuyama, M., Wang, Z., Yatabe, Y., Kiyono, T. and Inagaki, M.
    • Journal Title

      Mol. Biol. Cell

      Volume: 23 Pages: 1582-1592

    • Related Report
      2013 Final Research Report
  • [Journal Article] Evidence that formation of Vimentin/Mitogen-activated Protein Kinase (MAPK) complex mediates mast cell activation following FcεRI/CC chemokine receptor 1 cross-talk2012

    • Author(s)
      Toda, M., Kuo, C.H., Borman, S.K., Richardson, R.M., Inoko, A., Inagaki, M., Collins, A., Schneider, K. and Ono, S.J.
    • Journal Title

      J. Biol. Chem.

      Volume: 287 Pages: 24516-24524

    • Related Report
      2013 Final Research Report
  • [Journal Article] Trichoplein and Aurora A block aberrant primary cilia assembly in proliferating cells.2012

    • Author(s)
      Inoko, A., …., Inagaki, M.
    • Journal Title

      J. Cell Biol.

      Volume: 197 Issue: 3 Pages: 391-405

    • DOI

      10.1083/jcb.201106101

    • Related Report
      2012 Annual Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Novel regulation of checkpoint kinase 1 : Is checkpoint kinase 1 a good candidate for anti-cancer therapy?2012

    • Author(s)
      Goto, H., Izawa, I., Li, P. and Inagaki, M.
    • Journal Title

      Cancer Sci.

      Volume: (in press) Issue: 7 Pages: 1195-1200

    • DOI

      10.1111/j.1349-7006.2012.02280.x

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] P90 RSK arranges Chk1 in the nucleus for monitoring of genomic integrity during cell proliferation2012

    • Author(s)
      Li, P., Goto, H., Kasahara, K., Matsuyama, M., Wang, Z., Yatabe, Y., Kiyono, T., Inagaki, M.
    • Journal Title

      Mol. Biol. Cell

      Volume: 23 Issue: 8 Pages: 1582-1592

    • DOI

      10.1091/mbc.e11-10-0883

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] 細胞質分裂障害は染色体不安定性の亢進および細胞老化を誘導する2013

    • Author(s)
      田中宏樹
    • Organizer
      第65回日本細胞生物学会大会
    • Place of Presentation
      名古屋
    • Year and Date
      2013-06-21
    • Related Report
      2013 Final Research Report
  • [Presentation] Screening of novel Aurora-A-associated proteins to prevent primary cilia assembly at the centrosome in proliferating cells2013

    • Author(s)
      Goto, H
    • Organizer
      第65回日本細胞生物学会大会
    • Place of Presentation
      名古屋
    • Year and Date
      2013-06-20
    • Related Report
      2013 Final Research Report
  • [Presentation] Translocation of keratin-binding proteins between the cell-cell adhesion and the centrosome as the functional switch of differentiation and proliferation2013

    • Author(s)
      Inoko, A
    • Organizer
      第65回日本細胞生物学会大会
    • Place of Presentation
      名古屋
    • Year and Date
      2013-06-19
    • Related Report
      2013 Final Research Report
  • [Presentation] ビメンチンリン酸化の生理学的意義の解明2013

    • Author(s)
      稲垣昌樹
    • Organizer
      第13回日本蛋白質科学学会年会
    • Place of Presentation
      鳥取
    • Year and Date
      2013-06-12
    • Related Report
      2013 Final Research Report
  • [Presentation] Emerging role of the ubiquitin-proteasome pathway in primary cilia assembly2013

    • Author(s)
      Inagaki, M
    • Organizer
      The 25th CDB meeting "Cilia and centrosomes, from fertilization to cancer"
    • Place of Presentation
      Kobe, Japan
    • Related Report
      2013 Final Research Report
  • [Presentation] Intermediate filaments and site-and phosphorylation state-specific antibodies2012

    • Author(s)
      Inagaki, M
    • Organizer
      Global COE the 4th International Symposium
    • Place of Presentation
      Nagoya, Japan
    • Year and Date
      2012-11-15
    • Related Report
      2013 Final Research Report
  • [Presentation] Intermediate filaments and site-and phosphorylation state-specific antibodies2012

    • Author(s)
      Inagaki, M
    • Organizer
      Lecture at The University of Gothenburg
    • Place of Presentation
      Gothenberg, Sweden
    • Year and Date
      2012-09-24
    • Related Report
      2013 Final Research Report
  • [Presentation] Novel regulatory mechanism of Plk1 at mitosis2011

    • Author(s)
      Inagaki, M.
    • Organizer
      MEXT Priority Research Project International Symposium "Cell Division"
    • Place of Presentation
      ホテル大箱根(箱根)
    • Year and Date
      2011-06-29
    • Related Report
      2011 Annual Research Report
  • [Presentation] ビメンチンリン酸化の生理学的意義の解明

    • Author(s)
      稲垣昌樹
    • Organizer
      第13回日本蛋白質科学会年会
    • Place of Presentation
      とりぎん文化会館(鳥取市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 細胞質分裂障害は染色体不安定性の亢進および細胞老化を誘導する

    • Author(s)
      田中宏樹
    • Organizer
      第65回日本細胞生物学会大会
    • Place of Presentation
      ウインク愛知(名古屋)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Intermediate filaments and site- and phosphorylation state-specific antibodies.

    • Author(s)
      Inagaki, M.
    • Organizer
      Lecture at The University of Gothenburg
    • Place of Presentation
      The University of Gothenburg (Gothenburg, Sweden)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Intermediate filaments and site- and phosphorylation state-specific antibodies.

    • Author(s)
      Inagaki, M.
    • Organizer
      Global COE the 4th International Symposium
    • Place of Presentation
      ホテルルブラ王山(名古屋)
    • Related Report
      2012 Annual Research Report
  • [Book] Method for generation of antibodies specific for site-and post-translational modifications. Ossipow V. and Fischer N. (eds.), Monoclonal Antibodies: Methods and Protocols, Methods in Molecular Biology, vol. 11312014

    • Author(s)
      Goto, H. and Inagaki, M.
    • Publisher
      Springer Science+Business Media New York(in press)
    • Related Report
      2013 Final Research Report
  • [Book] Monoclonal Antibodies: Methods and Protocols, Second Edition, “Methods in molecular biology” series2013

    • Author(s)
      Goto, H.
    • Publisher
      Humana Press
    • Related Report
      2012 Annual Research Report
  • [Book] 「チェックポイントキナーゼ1(Chk1)の新規制御機構とがん」シグナル伝達研究最前線2012実験医学増刊Vol.30-NO.52012

    • Author(s)
      後藤英仁、稲垣昌樹
    • Publisher
      羊土社
    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.pref.aichi.jp/cancer-center

    • Related Report
      2013 Final Research Report
  • [Remarks] 愛知県がんセンターホームページ

    • URL

      http://www.pref.aichi.jp/cancer-center

    • Related Report
      2013 Annual Research Report
  • [Remarks] 愛知県がんセンターホームページ

    • URL

      http://www.pref.aichi.jp/cancer-center

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.pref.aichi.jp/cancer-center/ri/01bumon/08hatsugan_seigyo/index.html

    • Related Report
      2011 Annual Research Report

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Published: 2013-05-15   Modified: 2019-07-29  

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