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Splicing mechanisms of a novel split intron and evolution of the mRNA maturation process in eukaryotes

Research Project

Project/Area Number 23247038
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Evolutionary biology
Research InstitutionUniversity of Tsukuba

Principal Investigator

HASHIMOTO Tetsuo  筑波大学, 生命環境系, 教授 (50208451)

Co-Investigator(Kenkyū-buntansha) INAGAKI Yuji  筑波大学, 生命環境系, 准教授 (50387958)
NARA Takeshi  順天堂大学, 医学研究科, 准教授 (40276473)
IIDA Kei  京都大学, 医学研究科, 特定助教 (00387961)
Project Period (FY) 2011-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥30,550,000 (Direct Cost: ¥23,500,000、Indirect Cost: ¥7,050,000)
Fiscal Year 2014: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
Fiscal Year 2013: ¥11,700,000 (Direct Cost: ¥9,000,000、Indirect Cost: ¥2,700,000)
Fiscal Year 2012: ¥10,790,000 (Direct Cost: ¥8,300,000、Indirect Cost: ¥2,490,000)
Keywordsイントロン / トランススプライシング / スプライセオソーム / Giardia / 分子進化 / スプライソソーム / 機能進化
Outline of Final Research Achievements

We previously found novel split introns in the divergent protozoan parasite, Giardia intestinalis. In this project, we explored distribution of the split and ordinary introns in the Giardia genome. In addition to the four ordinary and four split introns that were already identified, we newly found two ordinary and one split introns. The ordinary introns were present in the separate two genes whose functions were unknown, while a split intron found in the RNA-dependent helicase p68 gene, the coding region of which was split, and N- and C-terminal portions of the gene were located distantly in the Giardia genome. To further investigate the splicing machinery of the split intron, and to compare the Giardia spliceosome which can splice both ordinary and split introns with other spliceosomes from diverse eukaryotes, we started biochemical analyses to purify intact spliceosomes from Giardia.

Report

(5 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (13 results)

All 2015 2014 2013 2012 2011

All Journal Article (7 results) (of which Peer Reviewed: 6 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (6 results) (of which Invited: 1 results)

  • [Journal Article] Phylogeny and evolution of apicoplasts and apicomplexan parasites2015

    • Author(s)
      Arisue N, Hashimoto T
    • Journal Title

      Parasitology International

      Volume: 64 Issue: 3 Pages: 254-259

    • DOI

      10.1016/j.parint.2014.10.005

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Secondary loss of a cis-spliced intron during the divergence of Giardia intestinalis assemblages2014

    • Author(s)
      Ryoma Kamikawa, Yuji Inagaki and Tetsuo Hashimoto
    • Journal Title

      BMC Research Notes

      Volume: 7 Issue: 1 Pages: 413-413

    • DOI

      10.1186/1756-0500-7-413

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Gene-content evolution in discobid mitochondria deduced from the phylogenetic position and complete mitochondrial genome of Tsukubamonas globosa.2014

    • Author(s)
      Kamikawa R, Kolisko M, Nishimura Y, Yabuki A, rown MW, Sohta A. Ishikawa, Ishida K, Roger AJ, Hashimoto T, Inagaki Y
    • Journal Title

      Genome Biology and Evolution

      Volume: 第6巻2号 Issue: 2 Pages: 306-315

    • DOI

      10.1093/gbe/evu015

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Parallel re-modeling of EF-la function in eukaryotic evolution : Divergent, low-expressed EF-1α genes co-occur with EFL genes in diverse distantly related eukaryotes.2013

    • Author(s)
      Kamikawa R, Brown MW, Nishimura Y, Sako Y, Heiss AA, Yubuki N, Gawryluk R, Simpson AGB, Roger AJ, Hashimoto T, Inagaki Y
    • Journal Title

      BMC Evolutionary Biology

      Volume: 13 Issue: 1 Pages: 131-131

    • DOI

      10.1186/1471-2148-13-131

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] RY-coding and non-homogeneous models ameliorate the maximum-likelihood inferences from nucleotide sequence data with parallel compositional heterogeneity.2012

    • Author(s)
      Ishikawa SA, Inagaki Y, Hashimoto T.
    • Journal Title

      Evolutionary Bioinformatics

      Volume: 8 Pages: 357-371

    • DOI

      10.4137/ebo.s9017

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Splintrons in Giardia intestinalis: Spliceosomal introns in a split form2011

    • Author(s)
      Kamikawa R
    • Journal Title

      Communicative & Integrative Biology

      Volume: 4 Pages: 454-456

    • Related Report
      2011 Annual Research Report
  • [Journal Article] A novel spliceosome-mediated trans-splicing can change our view on genome complexity of the divergent eukaryote Giardia intestinalis2011

    • Author(s)
      Ryoma Kamikawa, Inagaki Y, Hashimoto T
    • Journal Title

      Biophysical Reviews

      Volume: 3 Issue: 4 Pages: 193-197

    • DOI

      10.1007/s12551-011-0058-3

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] 43遺伝子系統解析により解明されたキネトプラスチダ類における寄生性形質獲得プロセス2015

    • Author(s)
      矢﨑裕規, 石川奏太, 久米慶太郎, 谷藤吾朗, 釜石隆, 橋本哲男, 稲垣祐司
    • Organizer
      第84回日本寄生虫学会大会
    • Place of Presentation
      杏林大学三鷹キャンパス, 東京
    • Year and Date
      2015-03-21 – 2015-03-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] フォルニカータ生物Kipferlia bialataの二者培養系の確立とゲノム・トランスクリプトーム解析2015

    • Author(s)
      髙林舜, 谷藤吾朗, 久米慶太郎, 稲垣祐司, 橋本哲男
    • Organizer
      第84回日本寄生虫学会大会
    • Place of Presentation
      杏林大学三鷹キャンパス, 東京
    • Year and Date
      2015-03-21 – 2015-03-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] A new strategy of organellar genome sequencing incorporating rolling circle amplification: protist mitochondria genomes2012

    • Author(s)
      Nishimura Y
    • Organizer
      Protist 2012
    • Place of Presentation
      Oslo (ノルウェー)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 寄生性真核微生物Giardiaの縮退ゲノムにおける複雑な遺伝子発現2011

    • Author(s)
      神川竜馬
    • Organizer
      第5回日本進化原生生物学研究会
    • Place of Presentation
      富山大学
    • Related Report
      2011 Annual Research Report
  • [Presentation] Spliceosomal introns in a split form: Another aspect of intron evolution in eukaryotes2011

    • Author(s)
      Kamikawa R
    • Organizer
      SMBE2011
    • Place of Presentation
      Kyoto University
    • Related Report
      2011 Annual Research Report
  • [Presentation] Trans-splicing of split introns is required for the expressions of indispensable genes in Giardia intestinalis2011

    • Author(s)
      Kamikawa R
    • Organizer
      IUMS2011 (International Union of Microbiological Societies 2011 Congress)
    • Place of Presentation
      Sapporo Convention Center
    • Related Report
      2011 Annual Research Report
    • Invited

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Published: 2013-05-15   Modified: 2019-07-29  

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