Analysis of formation of parasitic tissue connection using genomics infrastructure for tomato
| Project/Area Number |
23248005
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Horticulture/Landscape architecture
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| Research Institution | Osaka Prefecture University |
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Principal Investigator |
AOKI Koh 大阪府立大学, 生命環境科学研究科(系), 教授 (30344021)
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| Co-Investigator(Kenkyū-buntansha) |
YANO Kentaro 明治大学, 農学部, 准教授 (00446543)
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| Project Period (FY) |
2011-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥32,500,000 (Direct Cost: ¥25,000,000、Indirect Cost: ¥7,500,000)
Fiscal Year 2014: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2013: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2012: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
Fiscal Year 2011: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
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| Keywords | 寄生植物 / ネナシカズラ / ヤセウツボ / トマト / 維管束接続 / 細胞壁 / トランスクリプトーム / Orobanche aegyptiaca / Cuscuta japonica / 高速シークエンサー / オロバンキ / 遺伝子発現 / 通導組織 / 接続 / ゲノム |
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| Outline of Final Research Achievements |
We first established NGS-based genomic infrastructure by constructing databases TOMATOMICS and Plant Omics Data Center. We investigated the mechanisms how parasitic plants establish cellular connection to host plants. To this end, we used a stem parasitic plant, Cuscuta japonica, and a root parasitic plant, Orobanche aegyptiaca. Differentially expressed genes in the parasitic interface tissue formed between C. japonica and soybean were identified. We developed a bioinformatics method to classify RNA-Seq reads to either the parasite or the host, and analyzed gene expression profiles of the parasite and the host simultaneously. We discovered unforeseen coordination of gene expression between parasite and host plants, such as sequential expression of cell-wall degrading enzyme genes and cell-wall reconstitution genes. By using O. aegyptiaca parasitizing to tomato, we found that liquid-cultured callus segregated to parasitism-competent state and parasitism-incompetent states.
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Report
(5 results)
Research Products
(18 results)
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[Journal Article] Plant omics data center: an integrated web repository for interspecies gene expression networks with NLP-based curation.2015
Author(s)
Ohyanagi, H., Takano, T., Terashima, S., Kobayashi, M., Kanno, M., Morimoto, K., Matsumura, H., Sasaki, Y., Aya, K., Suwabe, K., Suzuki, G., Watanabe, M., Matsuoka, M., Yokoyama, K., Yano, K.
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Journal Title
Plant and Cell Physiology
Volume: 56
Issue: 1
Pages: 1-8
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Genome-wide analysis of intraspecific DNA polymorphism in ‘Micro-Tom’, a model cultivar of tomato (Solanum lycopersicum).2014
Author(s)
Kobayashi M, Nagasaki N, Garcia V, Just D, Bres C, Mauxion JP, Le Paslier MC, Brunel D, Suda K, Minakuchi Y, Toyoda A, Fujiyama A, Toyoshima H, Suzuki T, Igarashi K, Rothan C, Kaminuma E, Nakamura Y, Yano K, Aoki K.
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Journal Title
Plant and Cell Physiology
Volume: 55
Issue: 2
Pages: 445-454
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Presentation] CA Plot Viewer and Plant Omics Data Center: A GUI-based Tool for Gene Expression Network Construction and an Integrated Web Repository for Interspecies Gene Expression Networks with NLP-based Curation.2014
Author(s)
Ohyanagi H, Takano T, Terashima S, Kobayashi M, Kanno M, Morimoto K, Kanegae H, Ozaki S, Kudo T, Matsumura H, Sasaki Y, Saito M, Asano S, Yokoyama K, Aya K, Suwabe K, Suzuki G, Aoki K, Kubo Y, Watanabe M, Matsuoka M, Yano K.
Organizer
12th International Symposium on Rice Functional Genomics
Place of Presentation
Tucson, Arizona, USA.
Year and Date
2014-11-16 – 2014-11-19
Related Report
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