| Budget Amount *help |
¥32,500,000 (Direct Cost: ¥25,000,000、Indirect Cost: ¥7,500,000)
Fiscal Year 2014: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2013: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2012: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
Fiscal Year 2011: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
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| Outline of Final Research Achievements |
We first established NGS-based genomic infrastructure by constructing databases TOMATOMICS and Plant Omics Data Center. We investigated the mechanisms how parasitic plants establish cellular connection to host plants. To this end, we used a stem parasitic plant, Cuscuta japonica, and a root parasitic plant, Orobanche aegyptiaca. Differentially expressed genes in the parasitic interface tissue formed between C. japonica and soybean were identified. We developed a bioinformatics method to classify RNA-Seq reads to either the parasite or the host, and analyzed gene expression profiles of the parasite and the host simultaneously. We discovered unforeseen coordination of gene expression between parasite and host plants, such as sequential expression of cell-wall degrading enzyme genes and cell-wall reconstitution genes. By using O. aegyptiaca parasitizing to tomato, we found that liquid-cultured callus segregated to parasitism-competent state and parasitism-incompetent states.
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