Molecular structures, functional coordination, and cell dynamics of vacuolar proton pump and metal transporters

• Project/Area Number: 23248017
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Nagoya University
• Principal Investigator: MAESHIMA Masayoshi 名古屋大学, 生命農学研究科, 教授 (80181577)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥48,230,000 (Direct Cost: ¥37,100,000、Indirect Cost: ¥11,130,000); Fiscal Year 2013: ¥13,780,000 (Direct Cost: ¥10,600,000、Indirect Cost: ¥3,180,000); Fiscal Year 2012: ¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000); Fiscal Year 2011: ¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
• Keywords: 金属イオン / プロトンポンプ / イオン輸送体 / 液胞 / 分子構造 / 機能調節 / 膜輸送 / 亜鉛 / 金属ストレス / 植物 / 濃度調節 / 金属選択性

Research Abstract

We focussed our attention to the vacuolar proton H+-PPase and zinc transporter MTP1 and obtained the following information. (1) Individual biochemical role of 30 residues of MTP1 (FEBS J., 2012), (2) structural and ligand binding property of the His-rich loop of MTP1 (FEBS Open Bio, 2013), (3) biochemical dynamics of membrane transporters under Zn stress (Plant Physiol., 2011), functional role of H+-PPase for early development of seedlings (Plant Cell 2011, Plant Signal. Behav. 2012), and biochemical significance of interaction of H+-PPase and phosphatidylinositol phosphates (Plant Cell 2013).

Research Products

• [Journal Article] Characteristics of a root hair-less line of Arabidopsis thaliana under physiological stresses. (2014)
  • Author(s): Tanaka, N., Kato, M., Tomioka, R., Kurata, R., Fukao, Y., Aoyama, T., and Maeshima, M.
  • Journal Title: Journal of Experimental Botany Volume: 65 Issue: 6 Pages: 1497-1512
  • DOI: 10.1093/jxb/eru014
  • Peer Reviewed / Open Access
• [Journal Article] The ABCA9 transporter facilitates seeds storage lipid synthesis at the endoplasmic reticulum (2013)
  • Author(s): Kim, S., Yamaoka, Y., Ono, H., Kim, H., Shim, D., Maeshima, M., Martinoia, E., Cahoon, E.B., Nishida, I., and Lee, Y.
  • Journal Title: Proc. Natl. Acad. Sci. U.S.A. Volume: 110 Pages: 773-778
  • Peer Reviewed
• [Journal Article] Arabidopsis TWISTED DWARF1 functionally interacts with auxin exporter ABCB1 on the root plasma membrane (2013)
  • Author(s): Wang, B., Bailly, A., Zwiewka, M., Henrichs, S., Azzarello, E., Mancuso, S., Maeshima, M., Friml, J., Schulz, A., and Geisler, M.
  • Journal Title: Plant Cell Volume: 25 Pages: 202-214
  • Peer Reviewed
• [Journal Article] A Ca2+-binding protein PCaP2 located on the plasma membrane is involves in root hair development as a possible signal transducer (2013)
  • Author(s): Kato, M., Aoyama, T., and Maeshima, M.
  • Journal Title: Plant J. Volume: 74 Pages: 690-700
  • Peer Reviewed
• [Journal Article] Zinc-binding and structural properties of the histidine-rich loop of Arabidopsis thaliana vacuolar membrane zinc transporter MTP1 (2013)
  • Author(s): Tanaka, N., Kawachi, M., Fujiwara, T., and Maeshima, M.
  • Journal Title: FEBS Open Bio Volume: 3 Pages: 218-224
  • Peer Reviewed
• [Journal Article] A role of phosphatidylinositol 3,5-bisphosphate in vacuolar structure change in guard cells of closing stomata (2013)
  • Author(s): Bak, G., Lee, E.J., Lee, Y., Kato, M., Segami, S., Heven, S., Maeshima, M., Hwang, J.U., and Lee, Y.
  • Journal Title: Plant Cell Volume: 25 Pages: 2202-2216
  • Peer Reviewed
• [Journal Article] Local distribution of membrane proteins on vacuoles isolated from Arabidopsis suspension-cultured cells (2013)
  • Author(s): Yoshida, K., Ohnishi, M., Fukao, Y., Okazaki, Y., Song, C., Hayashi, F., Fujiwara, M., Nakanishi, Y., Saito, K., Shimmen, T., Suzaki, T., Fukaki, H., Maeshima, M., and Mimura, T.
  • Journal Title: Plant Cell Physiol. Volume: 54 Pages: 1571-1584
  • Peer Reviewed
• [Journal Article] Mn tolerance in rice is mediated by MTP8.1, a member of the cation diffusion facilitator family (2013)
  • Author(s): Chen, Z., Fujii, Y., Yamaji, N., Masuda, S., Takemoto, Y., Kamiya, T., Yusuyin, Y., Iwasaki, K., Kato, S., Maeshima, M., Ma, J.F., and Ueno, D.
  • Journal Title: J. Exp. Botany Volume: 64 Pages: 4375-4387
  • Peer Reviewed
• [Journal Article] Enhanced cell expansion in KRP2 overexpressor is mediated through increased V-ATPase activity (2013)
  • Author(s): Ferjani, A., Ishikawa, K., Asaoka, M., Ishida, M., Horiguchi, G., Maeshima, M., and Tsukaya, H.
  • Journal Title: Plant Cell Physiol. Volume: 54 Pages: 1989-1998
  • Peer Reviewed
• [Journal Article] Class III compensation, represented by KRP2 overexpression, depends on V-ATPase activity in proliferative cells (2013)
  • Author(s): Ferjani, A., Ishikawa, K., Asaoka, M., Ishida, M., Horiguchi, G., Maeshima, M., and Tsukaya, H.
  • Journal Title: Plant Signaling and Behavior Volume: 8
  • Peer Reviewed
• [Journal Article] The Ca2+-binding protein PCaP2 located on the plasma membrane is involved in root hair development as a possible signal transducer (2013)
  • Author(s): Kato, M., et. al.
  • Journal Title: Plant Journal Volume: (印刷中) Issue: 4 Pages: 690-700
  • DOI: 10.1111/tpj.12155
  • Peer Reviewed
• [Journal Article] Zinc-binding and structural properties of the histidine-rich loop of Arabidopsis thaliana vacuolar membrane zinc transporter MTP1. (2013)
  • Author(s): Tanaka, N., Kawachi, M., Fujiwara, T., and Maeshima, M.
  • Journal Title: FEBS Open Bio Volume: 3 Issue: 1 Pages: 218-224
  • DOI: 10.1016/j.fob.2013.04.004
  • Peer Reviewed
• [Journal Article] A role of phosphatidylinositol 3,5-bisphosphate in vacuolar structure change in guard cells of closing stomata. (2013)
  • Author(s): Bak, G., Lee, E.J., Lee, Y., Kato, M., Segami, S., Heven, S., Maeshima, M., Hwang, J.U., and Lee, Y.
  • Journal Title: Plant Cell Volume: 25 Issue: 6 Pages: 2202-2216
  • DOI: 10.1105/tpc.113.110411
  • Peer Reviewed
• [Journal Article] Ion gradients in xylem exudate and guttation fluid related to tissue ion levels along primary leaves of barley. (2013)
  • Author(s): Nagai Makiko, Ohnishi Miwa, Uehara Takeo, Yamagami Mutsumi, Miura Eiko, Kamakura Mai, Kitamura Akira, Sakaguchi Shuichi, Sakamoto Wataru, Shimmen Teruo, Fukaki Hidehiro, Reid Rob, J., Furukawa Akio and Mimura Tetsuro
  • Journal Title: Plant, Cell & Environment Volume: 36 Issue: 10 Pages: 1826-1837
  • DOI: 10.1093/pcp/pct107
  • Peer Reviewed
• [Journal Article] Mn tolerance in rice is mediated by MTP8.1, a member of the cation diffusion facilitator family. (2013)
  • Author(s): Chen, Z., Fujii, Y., Yamaji, N., Masuda, S., Takemoto, Y., Kamiya, T., Yusuyin, Y., Iwasaki, K., Kato, S., Maeshima, M., Ma, J.F., and Ueno, D.
  • Journal Title: Journal Experimental Botany Volume: 64 Issue: 14 Pages: 4375-4387
  • DOI: 10.1093/jxb/ert243
  • Peer Reviewed
• [Journal Article] Enhanced cell expansion in KRP2 overexpressor is mediated through increased V-ATPase activity. (2013)
  • Author(s): Ferjani, A., Ishikawa, K., Asaoka, M., Ishida, M., Horiguchi, G., Maeshima, M. and Tsukaya, H.
  • Journal Title: Plant Cell Physiology Volume: 54 Issue: 12 Pages: 1989-1998
  • DOI: 10.1093/pcp/pct138
  • Peer Reviewed
• [Journal Article] Class III compensation, represented by KRP2 overexpression, depends on V-ATPase activity in proliferative cells. (2013)
  • Author(s): Ferjani, A., Ishikawa, K., Asaoka, M., Ishida, M., Horiguchi, G., Maeshima, M., and Tsukaya, H.
  • Journal Title: Plant Signaling and Behavior Volume: 8
  • Peer Reviewed
• [Journal Article] The ABCA9 transporter facilitates seeds storage lipid synthesis at the endoplasmic reticulum. (2013)
  • Author(s): Kim, S., Yamaoka, Y., Ono, H., Kim, H., Shim, D., Maeshima, M., Martinoia, E., Cahoon, E.B., Nishida, I., Lee, Y.
  • Journal Title: Proc. Natl. Acad. Sci. U.S.A. Volume: 110 Issue: 2 Pages: 773-778
  • DOI: 10.1073/pnas.1214159110
  • Peer Reviewed
• [Journal Article] Arabidopsis TWISTED DWARF1 functionally interacts with auxin experter ABCB1 on the root plasma membrane. (2013)
  • Author(s): Wang, B., Bailly, A., Zwiewka, M., Henrichs, S., Azzarello, E., Mancuso, S., Maeshima, M., Friml, J., Schulz, A., Geisler, M.
  • Journal Title: Plant Cell Volume: 25 Issue: 1 Pages: 202-214
  • DOI: 10.1105/tpc.112.105999
  • Peer Reviewed
• [Journal Article] Regulation of pyrophosphate levels by the H+-PPase is central for proper resumption of early plant development (2012)
  • Author(s): Ferjani, A., Segami, S., Horiguchi, G., Sakata, A., Maeshima, M., and Tsukaya, H.
  • Journal Title: Plant Signaling & Behavior Volume: 7 Pages: 38-42
  • Peer Reviewed
• [Journal Article] Interaction of the trans-frame potyvirus protein P3N-PIPO with host protein PCaP1 facilitates potyvirus movement (2012)
  • Author(s): Vijayapalani, P., Maeshima, M., Nagasaki-Takekuchi, N., and Miller, W.A.
  • Journal Title: PLoS Pathogens Volume: 8
  • Peer Reviewed
• [Journal Article] Amino acid screening based on structural modeling identifies critical residues for the function, ion selectivity and structure of Arabidopsis MTP1 (2012)
  • Author(s): Kawachi, M., Kobae, Y., Kogawa, S., Mimura, T., Kramer, U., and Maeshima, M.
  • Journal Title: FEBS J. Volume: 279 Pages: 2339-2356
  • Peer Reviewed
• [Journal Article] Stimulation of root growth induced by aluminum in Quercus serrata Thunb. is related to activity of nitrate reductase and maintenance of IAA concentration in roots (2012)
  • Author(s): Tomioka, R., Takenaka, C., Maeshima, M., and Tezuka, T., Kojima, M., Sakakibara, H.
  • Journal Title: American J. Plant Sciences Volume: 3 Pages: 1619-1624
  • Peer Reviewed
• [Journal Article] Amino acid screening based on structural modeling identifies critical residues for function, ion selectivity and structure of Arabidopsis MTP1 (2012)
  • Author(s): Kawachi, M., et al
  • Journal Title: FEBS Journal Volume: (掲載確定) Issue: 13 Pages: 2339-2356
  • DOI: 10.1111/j.1742-4658.2012.08613.x
  • Peer Reviewed
• [Journal Article] Stimulation of root growth induced by aluminum in Quercus serrata Thunb. is related to activity of nitrate reductase and maintenance of IAA concentration in roots. (2012)
  • Author(s): Tomioka, R., Takenaka, C., Maeshima, M., and Tezuka, T., Kojima, M., Sakakibara, H.
  • Journal Title: American J. Plant Sciences Volume: 3 Issue: 11 Pages: 1619-1624
  • DOI: 10.4236/ajps.2012.311196
  • Peer Reviewed
• [Journal Article] Interaction of the trans-frame potyvirus protein P3N-PIPO with host protein PCaP1 facilitates potyvirus movement (2012)
  • Author(s): Vijayapalani, P., Maeshima, M., Nagasaki-Takekuchi, N., Miller, W.A.
  • Journal Title: PLoS Pathogens Volume: 8 Issue: 4 Pages: e1002639-e1002639
  • DOI: 10.1371/journal.ppat.1002639
  • Peer Reviewed
• [Journal Article] Amino acid screening based on structural modeling identifies critical residues for function, ion selectivity and structure of Arabidopsis MTP1 (2012)
  • Author(s): Kawachi, M., Kobae, Y., Kogawa, S., Mimura, T., Krämer, U., Maeshima, M.
  • Journal Title: FEBS Journal Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Vacuolar proton pumps and aquaporins involved in rapid internode elongation of deepwater rice (2011)
  • Author(s): Muto, Y., Segami, S., Hayashi, H., Sakurai, J., Murai, M., Hattori, Y., Ashikari, M., and Maeshima, M.
  • Journal Title: Biosci. Biotechnol. Biochem. Volume: 75 Pages: 114-122
  • NAID: 10027896894
  • Peer Reviewed
• [Journal Article] The iTRAQ analysis reveals mechanisms of growth defects due to excess zinc in Arabidopsis thaliana (2011)
  • Author(s): Fukao, Y., Ferjani, A., Tomioka, R., Nagasaki, N., Kurata, R., Nishimori, Y., Fujiwara, M., and Maeshima, M.
  • Journal Title: Plant Physiology Volume: 155 Pages: 1893-1907
  • Peer Reviewed
• [Journal Article] Chilling-induced changes of vacuolar proton pumps of hypocotyls from Vigna unguiculata (2011)
  • Author(s): Oliveira, L.M.N., Sobreira, A.C., Sobreira, A.C.M., Costa, J.H., Otoch, M.L.O., Maeshima, M., and Fernandes de Melo, D.
  • Journal Title: Plant Growth Regulation Volume: 64 Pages: 211-219
  • Peer Reviewed
• [Journal Article] Keep an eye on PPi : The vacuolar-type H+-pyrophosphatase regulates postgerminative development in Arabidopsis (2011)
  • Author(s): Ferjani, A., Segami, S., Horiguchi, G., Muto, Y., Maeshima, M., and Tsukaya, H.
  • Journal Title: Plant Cell Volume: 23 Pages: 2895-2908
  • Peer Reviewed
• [Journal Article] The small GTPase Rab5a is essential for intracellular transport of proglutelin from Golgi apparatus to the protein storage vacuole and endosomal membrane organization in developing rice endosperm (2011)
  • Author(s): Fukuda, M., Satoh-Cruz, M., Wen, L., Crofts, A.J., Sugino, A., Washida, H., Okita, T.W., Ogawa, M., Y. Kawagoe, Y., Maeshima, M., and Kumamaru, T.
  • Journal Title: Plant Physiol. Volume: 157 Pages: 632-644
  • Peer Reviewed
• [Journal Article] Keep an Eye on PPi : The Vacuolar-Type H^+-Pyrophosphatase Regulates Postgerminative Development in Arabidopsis (2011)
  • Author(s): Ferjani, A.
  • Journal Title: THE PLANT CELL Volume: 23(8) Issue: 8 Pages: 2895-2908
  • DOI: 10.1105/tpc.111.085415
  • Peer Reviewed
• [Presentation] Dimerization of GFPs fused to H+-pyrophosphatase influences the morphology and dynamics of vacuoles (2013)
  • Author(s): Segami, S. et al.
  • Organizer: International Workshop on Plant Membrane Biology
  • Place of Presentation: Okayama
• [Presentation] Active control mechanism of Arabidopsis thaliana Zn transporter MTP1. (2013)
  • Author(s): Kawachi, M., Tanaka, N., Tomioka, R., Kraemer, U., Maeshima, M.
  • Organizer: Annual Meeting of the Japan Biochemical Society, Symposium
  • Place of Presentation: Yokohama
  • Invited
• [Presentation] GFPなどの蛍光タグの多量体化がオルガネラの形態に与える影響 (2013)
  • Author(s): 瀬上紹嗣、牧野沙知、三宅愛、浅岡真理子、前島正義
  • Organizer: 日本生化学会大会
  • Place of Presentation: 横浜
• [Presentation] New functional residues of vacuolar H+-pyrophosphataase revealed by site-directed mutagenesis. (2013)
  • Author(s): Asaoka, M., Segami, S., Maeshima, M.
  • Organizer: International Workshop on Plant Membrane Biology
  • Place of Presentation: 倉敷市芸文館
• [Presentation] Dimerization of GFPs fused to H+-pyrophosphatase influences the morphology and dynamics of vacuoles. (2013)
  • Author(s): Segami, S., Makino, S., Miyake, A., Asaoka, M., Maeshima, M.
  • Organizer: International Workshop on Plant Membrane Biology
  • Place of Presentation: 倉敷市芸文館
• [Presentation] Zinc-binding and structural properties of the histidine-rich loop of Arabidopsis vacuolar membrane zinc transporter MTP1. (2013)
  • Author(s): Tanaka, N., Kawachi, M., Fujiwara, T., Maeshima, M.
  • Organizer: International Workshop on Plant Membrane Biology
  • Place of Presentation: 倉敷市芸文館
• [Presentation] 液胞膜バルブ構造の動態と生理的役割の解析 (2012)
  • Author(s): 瀬上紹嗣、牧野沙知、三宅愛、浅岡真理子、前島正義
  • Organizer: 日本植物生理学会2012年度年会
  • Place of Presentation: 京都産業大学
  • Year and Date: 2012-03-18
• [Presentation] 液胞膜プロトンポンプH^+-PPase機能欠失株と過剰発現株の生理学的特性の解析 (2012)
  • Author(s): 浅岡真理子、瀬上紹嗣、佐藤ちひろ、Ali Ferjani、前島正義
  • Organizer: 日本植物生理学会2012年度年会
  • Place of Presentation: 京都産業大学
  • Year and Date: 2012-03-18
• [Presentation] シロイヌナズナ亜鉛輸送体MTP1のイオン選択性および活性調節機構. (2012)
  • Author(s): 河内美樹、古川紗耶香、三村徹郎、Ute Kraemer、前島正義
  • Organizer: 日本生化学会大会
  • Place of Presentation: 福岡国際会議場
• [Presentation] シロイヌナズナにおけるユニークな亜鉛輸送体メンバーAtMTP12の解析 (2012)
  • Author(s): 日本分子生物学会大会
  • Organizer: 日本分子生物学会大会
  • Place of Presentation: 福岡国際会議場
• [Presentation] シロイヌナズナ液胞膜亜鉛輸送体AtMTP1のイオン選択性および活性調節機構解明 (2011)
  • Author(s): 河内美樹、三村徹郎、Ute Kraemer、前島正義
  • Organizer: 第37回生体エネルギー研究会討論会
  • Place of Presentation: 京都産業大学
  • Year and Date: 2011-12-21
• [Presentation] Membrane Transport Adaptation to High Temperatures : Aquaporins and H^+-Pyrophosphatase (2011)
  • Author(s): Maeshima, M., Tshuchira, A., Segami, S.
  • Organizer: The JSPS Colloquium in Stavanger on "Abiotic stress from genes to biosphere."
  • Place of Presentation: Stavanger, Norway(招待講演)
  • Year and Date: 2011-08-20
• [Presentation] 機能型液胞膜H^+-pyrophosphataseの発現株での解析 (2011)
  • Author(s): 瀬上紹嗣、牧野沙知、前島正義
  • Organizer: 日本生化学会大会
  • Place of Presentation: 京都国際会館
• [Book] Regulation of PPi levels through vacuolar membrane H+-pyrophosphatase. In Progress in Botany, Vol.75, ed. Luttge, Beyschlag and Cushman (2013)
  • Author(s): Ferjani, A., Segami, S., Asaoka, M. and Maeshima, M.
  • Publisher: Springer-Verlag, Heidelberg
• [Book] Progress in Botany, volume 75 (2013)
  • Author(s): Ferjani, A., Segami, S., Asaoka, M., and Maeshima, M.
  • Total Pages: 400
  • Publisher: Springer-Verlag
• [Book] Progress in Botany, Vol. 75 (Ed. By U. Luettge) (2013)
  • Author(s): erjani, A., Segami, S., Asaoka, M., and Maeshima, M.
  • Publisher: Springer-Verlag
• [Book] The role of membrane transport in the detoxification and accumulation of zinc in plants. In A Soil Biology Series: Detoxification of Heavy Metals (2011)
  • Author(s): Kawachi, M., Kobae, Y., Tomioka, R., and Maeshima, M.
  • Publisher: Springer-Verlag
• [Book] Application of radioisotope in biochemical analysis: Metal binding proteins and metal transporters. In Radioisotopes - Applications in Bio-Medical Science, Ed by Singh, N. (2011)
  • Author(s): Kawachi, M., Nagasaki-Takeuchi, N., Kato, M., and Maeshima, M.
  • Publisher: InTech
• [Book] A Soil Biology Series : Detoxification of Heavy Metals. (The role of membrane transport in the detoxification and accumulation of zinc in plants.) (2011)
  • Author(s): Kawachi, M., Kobae, Y., Tomioka, R., Maeshima, M.
  • Publisher: Springer-Verlag
• [Book] Radioisotopes-Applications in Bio-Medical Science (Application of radioisotope in biochemical analysis : Metal binding proteins and metal transporters) (2011)
  • Author(s): Kawachi, M., Nagasaki-Takeuchi, N., Kato, M., Maeshima, M.
  • Publisher: In Tech
• [Remarks]
  • URL: http://celld.agr.nagoya-u.ac.jp/index.html
• [Remarks]
  • URL: http://celld.agr.nagoya-u.ac.jp/index.html