Development of strigolactone function regulators and elucidation of strigolactne perception mechanisms
| Project/Area Number |
23248018
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bioproduction chemistry/Bioorganic chemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
ASAMI Tadao 東京大学, 農学生命科学研究科, 教授 (90231901)
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| Project Period (FY) |
2011-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥50,050,000 (Direct Cost: ¥38,500,000、Indirect Cost: ¥11,550,000)
Fiscal Year 2013: ¥15,730,000 (Direct Cost: ¥12,100,000、Indirect Cost: ¥3,630,000)
Fiscal Year 2012: ¥14,690,000 (Direct Cost: ¥11,300,000、Indirect Cost: ¥3,390,000)
Fiscal Year 2011: ¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000)
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| Keywords | 生理活性 / 植物 / 有機化学 / 農林水産物 / 遺伝子 / 受容体 / 植物ホルモン / ストリゴラクトン / ジベレリン / アゴニスト / アンタゴニスト / D14 / デブラノン / クロストーク / 創薬 / 農薬 / 植物生長調節剤 / DELLAタンパク質 / 加水分解 |
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| Outline of Final Research Achievements |
In plants, strigolactones (SLs) function as a phytohormone inhibiting shoot branching. An alpha/beta-hydrolase protein, DWARF14 (D14), and an F-box protein, DWARF3 (D3)/MAX2, have been shown to be involved in SL-signaling, although precise functions of them are largely unknown. Here we show the SL-dependent interaction of D14 with SLR1, a repressor of gibberellin signaling, the direct binding of SL to rice D14 (OsD14) protein, and the degradation of SL in vitro by D14. In addition, the X-ray crylstallographic analyses of OsD14 demonstrate the existence of open cavity sufficient for perceiving SLs, the binding of hydroxy D-ring, a putative product of SL hydrolysis by D14, to the cavity, and the amino acid residues critical for the interaction with the hydroxy D-ring. Our results present a novel model for the mechanism of SL recognition and signal transduction by D14. We found debranones as new chemicals possessing SL-like activity against the rice d10-1 mutant.
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Report
(4 results)
Research Products
(28 results)
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[Journal Article] Strigolactone regulates anthocyanin accumulation, acid phosphatases production and plant growth under low phosphate condition in Arabidopsis2015
Author(s)
Ito S, Nozoye T, Sasaki E, Imai M, Shiwa Y, Shibata-Hatta M, Ishige T, Fukui K, Ito K, Nakanishi H, Nishizawa NK, Yajima S, Asami T
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Journal Title
PLoS ONE
Volume: 10
Issue: 3
Pages: e0119724-e0119724
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Molecular mechanism of strigolactone perception by DWARF142013
Author(s)
Hidemitsu Nakamura, Yau-Lin Xue, Takuya Miyakawa, Feng Hou, Hui-Min Qin, Kosuke Fukui, Xuan Shi, Emi Ito, Shinsaku Ito, Seung-Hyun Park, Yumiko Miyauchi, Atsuko Asano, Naoya Totsuka, Takashi Ueda, Masaru Tanakura & Tadao Asami
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Journal Title
Nature Communications
Volume: 4
Issue: 1
Pages: 2613-2613
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] D14-SCFD3-dependent degradation of D53 regulates strigolactone signaling2013
Author(s)
Zhou F, Lin Q, Zhu L, Ren Y, Zhou K, Shabek N, Wu F, Mao H, Dong W, Gan L, Ma W, Gao H, Chen J, Yang C, Wang D, Tan J, Zhang X, Guo X, Wang J, Jiang L, Liu X, Chen W, Chu J, Yan C, Ueno K, Ito S, Asami T, Cheng Z, Wang J, Lei C, Zhai H, Wu C, Wang H, Zheng N and Wan J
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Journal Title
Nature
Volume: 504
Issue: 7480
Pages: 406-410
DOI
Related Report
Peer Reviewed
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