Development of strigolactone function regulators and elucidation of strigolactne perception mechanisms

• Project/Area Number: 23248018
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bioproduction chemistry/Bioorganic chemistry
• Research Institution: The University of Tokyo
• Principal Investigator: ASAMI Tadao 東京大学, 農学生命科学研究科, 教授 (90231901)
• Project Period (FY): 2011-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥50,050,000 (Direct Cost: ¥38,500,000、Indirect Cost: ¥11,550,000); Fiscal Year 2013: ¥15,730,000 (Direct Cost: ¥12,100,000、Indirect Cost: ¥3,630,000); Fiscal Year 2012: ¥14,690,000 (Direct Cost: ¥11,300,000、Indirect Cost: ¥3,390,000); Fiscal Year 2011: ¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000)
• Keywords: 生理活性 / 植物 / 有機化学 / 農林水産物 / 遺伝子 / 受容体 / 植物ホルモン / ストリゴラクトン / ジベレリン / アゴニスト / アンタゴニスト / D14 / デブラノン / クロストーク / 創薬 / 農薬 / 植物生長調節剤 / DELLAタンパク質 / 加水分解

Outline of Final Research Achievements

In plants, strigolactones (SLs) function as a phytohormone inhibiting shoot branching. An alpha/beta-hydrolase protein, DWARF14 (D14), and an F-box protein, DWARF3 (D3)/MAX2, have been shown to be involved in SL-signaling, although precise functions of them are largely unknown. Here we show the SL-dependent interaction of D14 with SLR1, a repressor of gibberellin signaling, the direct binding of SL to rice D14 (OsD14) protein, and the degradation of SL in vitro by D14. In addition, the X-ray crylstallographic analyses of OsD14 demonstrate the existence of open cavity sufficient for perceiving SLs, the binding of hydroxy D-ring, a putative product of SL hydrolysis by D14, to the cavity, and the amino acid residues critical for the interaction with the hydroxy D-ring. Our results present a novel model for the mechanism of SL recognition and signal transduction by D14. We found debranones as new chemicals possessing SL-like activity against the rice d10-1 mutant.

Research Products

• [Journal Article] Strigolactone regulates anthocyanin accumulation, acid phosphatases production and plant growth under low phosphate condition in Arabidopsis (2015)
  • Author(s): Ito S, Nozoye T, Sasaki E, Imai M, Shiwa Y, Shibata-Hatta M, Ishige T, Fukui K, Ito K, Nakanishi H, Nishizawa NK, Yajima S, Asami T
  • Journal Title: PLoS ONE Volume: 10 Issue: 3 Pages: e0119724-e0119724
  • DOI: 10.1371/journal.pone.0119724
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Molecular mechanism of strigolactone perception by DWARF14 (2013)
  • Author(s): Hidemitsu Nakamura, Yau-Lin Xue, Takuya Miyakawa, Feng Hou, Hui-Min Qin, Kosuke Fukui, Xuan Shi, Emi Ito, Shinsaku Ito, Seung-Hyun Park, Yumiko Miyauchi, Atsuko Asano, Naoya Totsuka, Takashi Ueda, Masaru Tanakura & Tadao Asami
  • Journal Title: Nature Communications Volume: 4 Issue: 1 Pages: 2613-2613
  • DOI: 10.1038/ncomms3613
  • Peer Reviewed / Open Access
• [Journal Article] D14-SCFD3-dependent degradation of D53 regulates strigolactone signaling (2013)
  • Author(s): Zhou F, Lin Q, Zhu L, Ren Y, Zhou K, Shabek N, Wu F, Mao H, Dong W, Gan L, Ma W, Gao H, Chen J, Yang C, Wang D, Tan J, Zhang X, Guo X, Wang J, Jiang L, Liu X, Chen W, Chu J, Yan C, Ueno K, Ito S, Asami T, Cheng Z, Wang J, Lei C, Zhai H, Wu C, Wang H, Zheng N and Wan J
  • Journal Title: Nature Volume: 504 Issue: 7480 Pages: 406-410
  • DOI: 10.1038/nature12878
  • Peer Reviewed
• [Journal Article] A novel mitochondrial DnaJ/Hsp40 family protein BIL2 promotes plant growth and resistance against environmental stress in brassinosteroid signaling (2013)
  • Author(s): Davaapurev Bekh-Ochir
  • Journal Title: Planta Volume: 237 Issue: 6 Pages: 1509-1525
  • DOI: 10.1007/s00425-013-1859-3
  • Peer Reviewed
• [Journal Article] Effects of strigolactone-biosynthesis inhibitor TIS108 on Arabidopsis (2013)
  • Author(s): shinsaku Ito
  • Journal Title: Plant Signal and Behavior Volume: 8
  • Peer Reviewed
• [Journal Article] Chemical screening of an inhibitor for gibberellin receptors based on a yeast two-hybrid system (2013)
  • Author(s): Jung-Min Yoon
  • Journal Title: Bioorganic and Medicinal Chemistry Letters Volume: 23 Issue: 4 Pages: 10960-10098
  • DOI: 10.1016/j.bmcl.2012.12.007
  • Peer Reviewed
• [Journal Article] Selective mimics of strigolactone actions and their potential use forcontrolling damage caused by root parasitic weeds (2013)
  • Author(s): Kosuke Fukui, Shinsaku Ito andTadao Asami
  • Journal Title: Molecular Plant Volume: 6(1) Issue: 1 Pages: 88-99
  • DOI: 10.1093/mp/sss138
  • Peer Reviewed
• [Journal Article] Effects of strigolactone-biosynthesis inhibitor TIS108 on Arabidopsis (2013)
  • Author(s): Shinsaku Ito
  • Journal Title: Plant Signal & Behavior Volume: 8
  • Peer Reviewed
• [Presentation] 機能選択的ストリゴラクトンミミック『デブラノン』の選択性に関する詳察 (2015)
  • Author(s): 福井 康祐、間下 大樹志、中村 英光、浅見 忠男
  • Organizer: 日本農芸化学会2015年度大会
  • Place of Presentation: 岡山大学
  • Year and Date: 2015-03-25
• [Presentation] ストリゴラクトン受容体阻害剤の探索 (2015)
  • Author(s): 間下大樹志、中村英光、浅見忠男
  • Organizer: 日本農薬学会第40回大会
  • Place of Presentation: 玉川大学
  • Year and Date: 2015-03-18
• [Presentation] Selective chemicals that mimic each of SL functions (2015)
  • Author(s): Tadao Asami
  • Organizer: 1st International Congress on Strigolactones
  • Place of Presentation: Wageningen, Holland
  • Year and Date: 2015-03-01 – 2015-03-06
  • Invited
• [Presentation] ストリゴラクトン誘導性転写因子の機能解析 (2014)
  • Author(s): 中村英光、永井優子、長江未有、増口 潔、山上あゆみ、光田展隆、高木 優、中野雄司、浅見忠男
  • Organizer: 植物化学調節学会第49回大会
  • Place of Presentation: 京都大学
  • Year and Date: 2014-10-18
• [Presentation] ストリゴラクトンミミック『デブラノン』の機能選択性に関する詳察 (2014)
  • Author(s): 福井康祐、山上大智、間下大樹志、中村英光、秋山康紀、浅見忠男
  • Organizer: 植物化学調節学会第49回大会
  • Place of Presentation: 京都大学
  • Year and Date: 2014-10-18
• [Presentation] Chemicals that negatively regulate plant disease resistance (2013)
  • Author(s): Tadao Asami
  • Organizer: VIPCA Plant Disease and Resistance Mechanism
  • Place of Presentation: Wiena, Austria
  • Invited
• [Presentation] ストリゴラクトンシグナル因子D14による加水分解と植物の分げつ抑制活性の関係 (2013)
  • Author(s): 戸塚直哉
  • Organizer: 日本農芸化学会2013年度大会
  • Place of Presentation: 仙台
  • Invited
• [Presentation] 枝分かれ抑制におけるストリゴラクトンとジベレリンのクロストーク (2013)
  • Author(s): 中村光英
  • Organizer: 日本農芸化学会2013年度大会
  • Place of Presentation: 仙台
  • Invited
• [Presentation] IAA mutants isolated by screening with IAA biosynthesis inhibitor (2012)
  • Author(s): Takuya Ando
  • Organizer: AUXIN2012
  • Place of Presentation: Kona, Hawaii
  • Invited
• [Presentation] Novel chemicals that control IAA biosynthesis and signaling (2012)
  • Author(s): Ikuya Shirai
  • Organizer: AUXIN2012
  • Place of Presentation: Kona, Hawaii
  • Invited
• [Presentation] P450阻害剤処理に伴うストリゴラクトン内生量の変動
  • Author(s): 戸塚直哉
  • Organizer: 農薬デザイン研究会
  • Place of Presentation: お茶の水ジュラクホテル
• [Presentation] ストリゴラクトンにより誘導される転写因子の光応答シグナルにおける機能の解析
  • Author(s): 永井優子
  • Organizer: 日本農芸化学会2013年度大会
  • Place of Presentation: 東北大学川内校舎
• [Presentation] 枝分かれ抑制におけるストリゴラクトンとジベレリンのクロストーク
  • Author(s): 中村英光
  • Organizer: 日本農芸化学会2013年度大会
  • Place of Presentation: 東北大学川内校舎
• [Presentation] ストリゴラクトン生合成阻害剤を利用したイネの枝分かれ抑制変異体の探索
  • Author(s): 山上大智
  • Organizer: 日本農芸化学会2013年度大会
  • Place of Presentation: 東北大学川内校舎
• [Presentation] シロイヌナズナにおけるリン酸欠乏とストリゴラクトンの関係
  • Author(s): 伊藤晋作
  • Organizer: 日本農芸化学会2013年度大会
  • Place of Presentation: 東北大学川内校舎
• [Presentation] シロイヌナズナの根の形態形成における新規ストリゴラクトンミミックの作用解析
  • Author(s): 福井康祐
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山大學
• [Presentation] ストリゴラクトンシグナル因子D14による加水分解と 植物の分げつ抑制活性の関係
  • Author(s): 戸塚直哉
  • Organizer: 日本農芸化学会2013年度大会
  • Place of Presentation: 東北大学川内校舎
• [Presentation] ストリゴラクトン生合成阻害剤を用いた変異体の探索
  • Author(s): 山上大智
  • Organizer: 植物化学調節学会第47回大会
  • Place of Presentation: 山形大学鶴岡キャンパス
• [Presentation] 新規ストリゴラクトンアナログdebranoneのシロイヌナズナの根の形態への影響
  • Author(s): 福井康祐
  • Organizer: 植物化学調節学会第47回大会
  • Place of Presentation: 山形大学鶴岡キャンパス
• [Book] ストリゴラクトン受容体の構造とシグナル伝達のしくみ (2015)
  • Author(s): 中村英光、宮川拓哉、田之倉優、浅見忠男
  • Publisher: 日本農芸化学会