Mechanisms of disease development in pine wilt disease based on the interaction of biomolecules

• Project/Area Number: 23248024
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Forest science
• Research Institution: Forestry and Forest Products Research Institute
• Principal Investigator: SAHASHI NORIO 独立行政法人森林総合研究所, 森林微生物研究領域, 領域長 (10202102)
• Co-Investigator(Kenkyū-buntansha): KIKUTI Taisei 宮崎大学, 医学部, 准教授 (20353659) ; AKIBA Mitsuteru (独)森林総合研究所, 森林微生物研究領域, 主任研究員 (50353553) ; KANZAKI Natsumi (独)森林総合研究所, 森林微生物研究領域, 主任研究員 (70435585) ; YOKOI Toshiro (独)森林総合研究所, 森林微生物研究領域, 主任研究員 (60378867) ; WATANABE Atsushi 九州大学, 農学研究科, 准教授 (10360471) ; HIRAO Tomonori (独)森林総合研究所, 森林バイオ研究センター, 主任研究員 (90457763) ; FUTAI Kazuyoshi 京都大学, 農学研究科, 教授 (50165445) ; UEDA Mitsuyoshi 京都大学, 農学研究科, 教授 (90183201) ; TAKEUCHI Yuko 京都大学, 農学研究科, 助教 (80452283) ; YAMADA Toshihiro 東京大学, 農学生命科学研究科, 教授 (30332571) ; KUSUMOTO Dai 東京大学, 農学生命科学研究科, 講師 (80540608) ; HASEGAWA Koichi 中部大学, 応用生物学部, 講師 (10609837)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥49,790,000 (Direct Cost: ¥38,300,000、Indirect Cost: ¥11,490,000); Fiscal Year 2013: ¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000); Fiscal Year 2012: ¥15,600,000 (Direct Cost: ¥12,000,000、Indirect Cost: ¥3,600,000); Fiscal Year 2011: ¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
• Keywords: マツ材線虫病 / マツノザイセンチュウ / 発病機構 / 生体分子 / 感染症 / 宿主－寄生者相互作用 / 網羅的解析 / マツ林線虫病 / 宿主-寄生者相互作用

Research Abstract

To understand the pathogenicity (or virulence) of Bursaphelencus xylophilus (a causal pathogen of pine wilt disease) and the mechanisms of resistance (or susceptibility) of pine trees, physiological, histoathological and molecular biological studies were carried out. High quality draft genome sequence of Bursaphelenchus xylophilus was completed. Furthermore, many important factors associated with disease development were identified in both nematode and pine tree. The results presented here will contribute for further understanding the mechanisms of pine wilt disease development.

Research Products

• [Journal Article] Integrative diagnosis of the needle nematode Longidorus jonesi affecting forest in soutern Japan (2014)
  • Author(s): Palomares-Rius, J.E., Kikuchi, T., Cantalapiedra-Navarrete, C., and Castillo, P.
  • Journal Title: Forest Pathology Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Signatures of adaptation to plant parasitism in nematode genomes (2014)
  • Author(s): Bird, D.M., Jones, J.T., Opperman, C.H., Kikuchi, T., and Danchin, E.G.
  • Journal Title: Parasitology Pages: 1-14
  • Peer Reviewed
• [Journal Article] The genome and life-stage specific transcriptomes of Globodera pallida elucdate key aspects of plant parasitism by a cyst nematode (2014)
  • Author(s): Cotton, J., Lilley, C., Jones, L., Kikuchi, T., Reid, A., Thorpe, P., Tsai, I., Beasley, H., Blok, V., Cock, P., Eves-van den Akker, S., Holroyd, N., Hunt, M., Mantelin, S., Naghra, H., Pain, A., Palomares-Rius, J., Zarowiecki, M., Berriman, M., Jones, J., and Urwin, P.
  • Journal Title: Genome Biology Volume: 15巻
  • Peer Reviewed
• [Journal Article] Characterisation of the transcriptome of Aphelenchoides besseyi and identification of a GHF 45 cellulase (2014)
  • Author(s): Kikuchi, T., Helder, J., Cock, P.J., and Jones, J.T.
  • Journal Title: Nematology Volume: 16巻 Pages: 99-107
  • Peer Reviewed
• [Journal Article] Comparison of histological responses and tissue damage expansion between rsistant and susceptible Pinus thunbergii ifected with pine wood nematode Bursaphelenchus xylophilus (2014)
  • Author(s): Kusumoto, D., Yonemichi, T., Inoue, H., Hirao, T., Watanabe, A., and Yamada, T.
  • Journal Title: Journal of Forest Research Volume: 19巻 Pages: 285-294
  • Peer Reviewed
• [Journal Article] Distribution and evoltion of Glycoside Hydrolase Family 45 cellulases in Nematodes and Fungi (2014)
  • Author(s): Palomares-Rius, J.E., Hirooka, Y., Tsai, I.J., Masuya, H., Hino, A., Kanzaki, N., Jones, J.T., and Kikuchi, T.
  • Journal Title: BMC Evolutionary Biology Volume: 14巻 Pages: 69-69
  • Peer Reviewed
• [Journal Article] Bursaphelenchus niphades n. sp. (Tylenchina : Aphelenchoididae) amensally associated with Niphades variegatus (Roelofs) (Coleoptera: Curculionidae) (2014)
  • Author(s): Tanaka, S., Tanaka, R., Akiba, M., Aikawa, T., Maehara, N., Takeuchi, Y., Kanzaki, N.
  • Journal Title: Nematology Volume: 16巻 Pages: 259-281
  • Peer Reviewed
• [Journal Article] Signatures of adaptation to plant parasitism in nematode genomes. (2014)
  • Author(s): Bird, D.M., Jones, J.T., Opperman, C.H., Kikuchi, T., and Danchin, E.G.
  • Journal Title: Parasitology Volume: 無し Issue: S1 Pages: 1-14
  • DOI: 10.1017/s0031182013002163
  • Peer Reviewed
• [Journal Article] Characterisation of the transcriptome of Aphelenchoides besseyi and identification of a GHF 45 cellulase. (2014)
  • Author(s): Kikuchi, T., Helder, J., Cock, P.J., and Jones, J.T.
  • Journal Title: Nematology Volume: 16 Issue: 1 Pages: 99-107
  • DOI: 10.1163/15685411-00002748
  • Peer Reviewed
• [Journal Article] Comparison of histological responses and tissue damage expansion between resistant and susceptible Pinus thunbergii infected with pine wood nematode Bursaphelenchus xylophilu (2014)
  • Author(s): Kusumoto, D., Yonemichi, T., Inoue, H., Hirao, T., Watanabe, A., and Yamada, T.
  • Journal Title: Journal of Forest Research Volume: 19 Issue: 2 Pages: 285-294
  • DOI: 10.1007/s10310-013-0417-y
  • Peer Reviewed
• [Journal Article] Pine Wood Nematode, Bursaphelenchus xylophilus (2013)
  • Author(s): Futai, K.
  • Journal Title: Annual Review of Phytopathology Volume: 51巻 Pages: 61-83
  • Peer Reviewed
• [Journal Article] REAPR : a universal tool for genome assembly evalution (2013)
  • Author(s): Hunt, M., Kikuchi, T., Sanders, M., Newbold, C., Berriman, M., and Otto, T.D.
  • Journal Title: Genome Biology Volume: 14巻
  • Peer Reviewed
• [Journal Article] Top 10 plant-parasitic nematodes in molecular plant pathology (2013)
  • Author(s): Jones, J.T., Haegeman, A., Danchin, E.G.J., Gaur, H.S., Helder, J., Jones, M.G.K., Kikchi, T.,Manzanilla-Lopez,R., Palomares-Rius, J.E., Wesemael, W.M.L., and Perry, R.N.
  • Journal Title: Molecular Plant Pathology Volume: 14巻 Pages: 946-961
  • Peer Reviewed
• [Journal Article] The genome and transcriptome of Haemonchus contortus, a key model parasite for drug and vaccine discovery (2013)
  • Author(s): Laing, R., Kikuchi, T., Martinelli, A., Tsai, I., Beech, R., Redman, E., Holroyd, N., Bartley, D., Beasley, H., Britton, C., Curran, D., Devaney, E., Gilabert, A., Hunt, M., Jackson, F., Johnston, S., Kryukov, I., Li, K., Morrson, A., Reid, A., Sargison, N., Saunders, G., Wasmuth, J., Wolstenholme, A., Berriman, M., Gilleard, J., and Cotton, J.
  • Journal Title: Genome Biology Volume: 14巻
  • Peer Reviewed
• [Journal Article] Omics fields of study related to plant-parasitic nematodes (2013)
  • Author(s): Palomares-Rius, J.E., and Kikuchi, T.
  • Journal Title: Journal of Integrated OMICS Volume: 3巻 Pages: 1-10
  • Peer Reviewed
• [Journal Article] Pine Wilt Disease caused by Bursaphelenchus xylophilus, a new disease threatening Spaish forests (2013)
  • Author(s): Palomares-Rius, J.E., and Kikuchi, T.
  • Journal Title: Vida Rural Volume: 358巻 Pages: 74-79
  • Peer Reviewed
• [Journal Article] Secretome analysis of the pine wood nematode Bursaphelenchus xylophilus reveals the tagled roots of parasitism and its potential for molecular mimicry (2013)
  • Author(s): Shinya, R., Morisaka, H., Kikuchi, T., Takeuchi, Y., Ueda, M., Futai, K.
  • Journal Title: PLoS ONE Volume: 8巻
  • Peer Reviewed
• [Journal Article] Making headway in undestanding pine wilt disease : What do we peceive in the post genomic era? (2013)
  • Author(s): Shinya, R., Morisaka, H., Takeuchi, Y., Futai, K., Ueda, M.
  • Journal Title: Journal of Bioscience and Bioengineering Volume: 116巻 Pages: 1-8
  • Peer Reviewed
• [Journal Article] Summrizing Specific Profiles in Illumina Sequencing from Whole-Genome Amplified DNA (2013)
  • Author(s): Tsai, I.J., Hunt, M., Holroyd, N., Huckvale, T., Berriman, M., and Kikuchi, T.
  • Journal Title: DNA Research Volume: in press Issue: 3 Pages: 243-254
  • DOI: 10.1093/dnares/dst054
  • Peer Reviewed
• [Journal Article] Characterization of bacterial communities associated with the pine sawyer beetle Monochamus galloprovincialis, the insect vector of the pinewood nematode Bursaphelenchus xylophilus (2013)
  • Author(s): Vicente, C.S.L., Nascimento, F., Espada, M., Barbosa, P., Hasegawa, K., Mota, M. and Oliveira, S.
  • Journal Title: FEMS Micrbiology Letters Volume: 347巻 Pages: 130-139
  • Peer Reviewed
• [Journal Article] Pinewood nematode-associated bacteria contribute to oxidative stress rstance of Bursaphelenchus xylophilus (2013)
  • Author(s): Vicente, C.S.L., Ikuyo, Y., Mota, M., Hsegawa, K.
  • Journal Title: BMC Mcrobiology Volume: 13巻 Pages: 299-299
  • Peer Reviewed
• [Journal Article] Making headway in understanding pine wilt disease : What do we perceive in the postgenomic era? (2013)
  • Author(s): Ryoji Shinya, Hironobu Morisaka, Yuko Takeuchi, Kazuyoshi Futai, Mitsuyoshi Ueda
  • Journal Title: Journal of Bioscience and Bioengineering Volume: 116 Issue: 1 Pages: 1-8
  • DOI: 10.1016/j.jbiosc.2013.01.003
  • Peer Reviewed
• [Journal Article] Secretome Analysis of the Pine Wood Nematode Bursaphelenchus xylophilus Reveals the Tangled Roots of Parasitism and Its Potential for Molecular Mimicry (2013)
  • Author(s): Shinya R, Morisaka H, Kikuchi T, Takeuchi Y, Ueda M, Futai K
  • Journal Title: PLoS ONE Volume: なし Issue: 6 Pages: e67377-e67377
  • DOI: 10.1371/journal.pone.0067377
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Mortality of shaded pine trees inoculated with virulent and less-virulent isolates of pine wood nematodes (2012)
  • Author(s): Kanzaki, N., Tanaka, R. and Sahashi, N.
  • Journal Title: Environmental Etomology Volume: 41巻 Pages: 828-832
  • Peer Reviewed
• [Journal Article] Characteization of resistance to pine wood nematode infection in Pinus thunbergii using suppression subtractive hybridization (2012)
  • Author(s): Hirao T, Fukatsu E, Watanabe A.
  • Journal Title: BMC Plant Biology Volume: 12巻 Pages: 13-13
  • Peer Reviewed
• [Journal Article] Establishment of a set of inbred strains of the pine wood nemaode, Bursaphelenchus xylophilus (Aphelenchida : Aphelenchoididae), and evdence of their varying levels of virulence (2012)
  • Author(s): Shinya R., Takeuchi, Y., Ichimura, K., Takemoto, S., Futai, K.
  • Journal Title: Applied Entomology and Zoology Volume: 47巻 Pages: 341-350
  • Peer Reviewed
• [Journal Article] Simple and quick methods for nematode DNA preparation (2012)
  • Author(s): Tanaka, R., Kikuchi, T., Aikawa, T., and Kanzaki, N.
  • Journal Title: Applied Entmology and Zoology Volume: 47巻 Pages: 291-294
  • NAID: 40019400664
  • Peer Reviewed
• [Journal Article] Virlence of Bursaphelenchus xylophilus islated from naturally infested pine forests to five resistant families of Pinus thunbergii (2012)
  • Author(s): Akiba, M., Ishihara, M., Sahashi, N., Nakmura, K., Ohira, M. and Toda, T.
  • Journal Title: Plant Disease Volume: 96巻 Pages: 249-252
  • Peer Reviewed
• [Journal Article] Mortality of shaded pine trees inoculated with virulent and less-virulent isolates of pine wood nematodes (2012)
  • Author(s): Natsumi Kanzaki, Ryusei Tanaka and Norio Sahashi
  • Journal Title: Environmental Entomology Volume: 41 Issue: 4 Pages: 828-832
  • DOI: 10.1603/en12031
  • Peer Reviewed
• [Journal Article] Ecology of Armillaria species on conifers in Japan (2011)
  • Author(s): Hasegawa, E., Ota, Y., Hattori, T., Sahashi, N., and Kikuchi, T.
  • Journal Title: Forest Pathoogy Volume: 41巻 Pages: 429-437
  • Peer Reviewed
• [Journal Article] Detection of Bursaphelenchus xylophilus from old discs of dead Pinus armandii var. amamiana trees using a new detection kit (2011)
  • Author(s): Kanetani, S., Kikuchi, T., Akiba, M., Nakmura, K., Ikegame, H., and Tetsuka, K.
  • Journal Title: Forest Pathology Volume: 41巻 Pages: 387-391
  • Peer Reviewed
• [Journal Article] Genomic Insights into the Origin of Parasiism in the Emerging Plant Pathogen Bursaphelenchus xylophilus (2011)
  • Author(s): Kikuchi, T., Cotton, J.A., Dalzell, J.J., Hsegawa, K., Kanzaki, N., McVeigh, P., Takanashi, T., Tsai, I.J., Assefa, S.A., Cock, P.J.A., Otto, T.D., Hunt, M., Reid, A.J., Sanchez-Flores, A., Tsuchihara, K., Yokoi, T., Larsson, M.C., Miwa, J., Maule, A.G., Sahashi, N., Jones, J.T., and Berriman, M.
  • Journal Title: PLoS Pathgens Volume: 7巻
  • Peer Reviewed
• [Journal Article] 材線虫病抵抗性アカマツにおけるマツノザイセンチュウの移動パターン (2011)
  • Author(s): 松井優一・山田利博
  • Journal Title: 樹木医学研究 Volume: 15巻 Pages: 107-108
  • NAID: 110009627318
  • Peer Reviewed
• [Journal Article] Genomic insights into the origin of parasitism in the emerging plant pathogen Bursaphelenchus xylophilus (2011)
  • Author(s): Kikuchi, T., Cotton, J., Dalzell, J.J., Hasegawa, K., Kanzaki, N., McVeigh, P., Takanashi, T., Tsai, I.J., Assefa, S.A., Cook, P.J.A., Otto, T.D., Hunt, M., Reid, A.J., Sanchez-Flores, A., Tsuchihara, K., Yokoi, T., Larsson, M.C, Miwa, J., Maule, A.G., Sahashi, N., Jones, J.T., Berriman, M.
  • Journal Title: PloS Pathogens Volume: 7 Issue: 9 Pages: e1002219-e1002219
  • DOI: 10.1371/journal.ppat.1002219
  • Peer Reviewed
• [Journal Article] Virulence of Bursaphelenchus xylophilus Isolated from Naturally Infested Pine Forests to Five Resistant Families of Pinus thunbergii (2011)
  • Author(s): Mitsuteru Akiba, Makoto Ishihara, Norio Sahashi, Katsunori Nakamura
  • Journal Title: Plant Disease Volume: 96(2) Issue: 2 Pages: 249-252
  • DOI: 10.1094/pdis-12-10-0910
  • Peer Reviewed
• [Journal Article] 材線虫病抵抗性アカマツにおけるマツノザイセンチュウの移動パターン.樹木医学研究 (2011)
  • Author(s): 松井優一・山田利博
  • Journal Title: 樹木医学研究 Volume: 15(3) Pages: 107-108
• [Presentation] RAD-seqを用いたマツノザイセンチュウ組み換え近交系の重要形質関連遺伝子群の探索 (2014)
  • Author(s): 加藤徹朗・金子彰・八杉公基・本庄三恵・永野惇・工藤洋・森一樹・久原哲・二井一禎・竹内祐子
  • Organizer: 第125回日本森林学会大会
  • Place of Presentation: 埼玉
  • Year and Date: 2014-03-29
• [Presentation] 病原力が異なるマツノザイセンチュウ系統間での酸化ストレス耐性の差異 (2014)
  • Author(s): 浴野泰甫・加藤徹朗・金子彰・竹内祐子
  • Organizer: 第125回日本森林学会大会
  • Place of Presentation: 埼玉
  • Year and Date: 2014-03-29
• [Presentation] 組み換え近交系を用いたマツノザイセンチュウにおける重要形質のゲノム解析 (2014)
  • Author(s): 加藤徹朗・金子彰・本庄三恵・永野惇・工藤洋・森一樹・菊地泰生・久原哲・二井一禎・竹内祐子
  • Organizer: 第58回日本応用動物昆虫学会大会
  • Place of Presentation: 高知
  • Year and Date: 2014-03-28
• [Presentation] A Time-Course Comparative Microarray Analysis of Different Genotypes in Japanese Black Pine (Pinus thunbergii) during Attack By Virulent Nematode (Bursaphelenchus xylophilus) and Avirulent Nematode (B. mucronatus) (2014)
  • Author(s): Hirao,T., Watanabe, A.
  • Organizer: Plant and Animal Genome XXII
  • Place of Presentation: サンディエゴ
  • Year and Date: 2014-01-13
• [Presentation] マツ材線虫病抵抗性クロマツと感受性クロマツの解剖学的比較 (2013)
  • Author(s): 楠本 大
  • Organizer: 第124回日本森林学会大会
• [Presentation] 房総半島におけるヒメコマツ実生苗のマツ材線虫病抵抗性について (2013)
  • Author(s): 軽込 勉
  • Organizer: 第124回日本森林学会大会
• [Presentation] 千葉県の海岸砂地と内陸畑土でのマツノザイセンチュウ接種試験による生存率の関係 (2013)
  • Author(s): 福原一成
  • Organizer: 第124回日本森林学会大会
• [Presentation] Comparison of gene expression profiles between resistance and susceptibility in Pinus thunbergii to pine wood nematode infection (2012)
  • Author(s): Hirao T.
  • Organizer: Abstracts 3rd Meeting of IUFRO Working Unit 7.03.12 "Alien invasive species and international trade"
• [Presentation] The resistance factors of Japanese red pines to pine wilt disease. (2012)
  • Author(s): Matsui Y．
  • Organizer: Abstracts 3rd Meeting of IUFRO Working Unit 7.03.12 "Alien invasive species and international trade"
• [Presentation] プロテオームデータ処理のこれまでとこれから (2012)
  • Author(s): 新屋良治
  • Organizer: バイオアナリシスシンポジウム
  • Invited
• [Presentation] マツ材線虫病抵抗性アカマツにおける抵抗性要因 (2012)
  • Author(s): 松井優一
  • Organizer: 樹木医学会17回大会
• [Presentation] Pathogenicity of the pine wood nematode, Bursaphelenchus xylophilus, revealed by proteomic analysis (2011)
  • Author(s): Kazuyoshi Futai, Ryoji Shinya, Yuko Takeuchi, Mitsuyoshi Ueda
  • Organizer: 3rd International Symposium on Frontiers in Agriculture Proteome Research-Contribution of proteomics technology in agricultural sciences
  • Place of Presentation: Tsukuba International Congress Center(EPOCHAL)(つくば)(招待講演)
  • Year and Date: 2011-11-10
• [Presentation] マツノザイセンチュウ研究の新たな展開 (2011)
  • Author(s): 新屋良治
  • Organizer: 日本線虫学会第19回大会公開シンポジウム『線虫学のパイオニアから学ぶサイエンスのこれから』
  • Place of Presentation: 京都市国際交流会館(京都)(招待講演)
  • Year and Date: 2011-09-16
• [Presentation] A Time-Course Comparative Microarray Analysis of Different Genotypes in Japanese Black Pine (Pinus thunbergii) during Attack By Virulent Nematode (Bursaphelenchus xylophilus)
  • Author(s): Hirao,T., Watanabe, A.
  • Organizer: Plant and Animal Genome
  • Place of Presentation: サンディエゴ
• [Presentation] Bacterial flora and its association with the pine wood nematode (Bursaphelenchus xylophilus).
  • Author(s): Kato, T., Futai, K., Takeuchi, Y.
  • Organizer: IUFRO 2013 -Pine Wilt Disease Conference
  • Place of Presentation: Braunschweig, Germany
• [Presentation] Whole genome resequencing and SNP detection toward understanding population structure and parasitism of Bursaphelenchus xylophilus
  • Author(s): Paromares-Rius, J.E., 金子彰・田中龍聖・秋庭満輝・竹内祐子・菊地泰生
  • Organizer: 日本線虫学会第21回大会
  • Place of Presentation: 唐津
• [Book] 「生活環境と森林病害」、南基泰・山木昭平編環境生物学序論 (2013)
  • Author(s): 風媒社
  • Publisher: 風媒社
• [Book] Chapter 6. Pine Wilt Disease and Other Nematode Diseases. pp. 115-127. In: Infectious Forest Diseases. Gonthier, P., Nicolotti, G. (Eds.) (2013)
  • Author(s): Kamata, N., Takeuchi, Y
  • Total Pages: 622
  • Publisher: CABI Book
• [Book] 「生活環境と森林病害」、南基泰・山木昭平 編 環境生物学序論 (2013)
  • Author(s): 長谷川浩一
  • Total Pages: 259
  • Publisher: 風媒社
• [Book] Chapter 6. Pine Wilt Disease and Other Nematode Diseases. Pp. 115-127 In: Infectious Forest Diseases. Gonthier, P., Nicolotti, G. (Eds.) (2013)
  • Author(s): Kamata, N., Takeuchi, Y.
  • Total Pages: 672
  • Publisher: CABI Book
• [Book] 「線虫が切り拓く生物学 ―そしてモデル生物から非モデル生物へ」、二井一禎・竹内祐子・山崎理正編微生物生態学への招待森をめぐるミクロな世界 ‐ (2012)
  • Author(s): 長谷川浩一
  • Publisher: 京都大学学術出版
• [Book] 「第13章 感染しても枯れない? ―白黒つかないマツと線虫の関係」、二井一禎・竹内祐子・山崎理正(編)微生物生態学への招待 ―森をめぐるミクロな世界 (2012)
  • Author(s): 竹内祐子
  • Publisher: 京都大学学術出版会