Immunological and genomic studies on interation between kuruma shrimp Immune and biodefense and microbial pathogens

• Project/Area Number: 23248029
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General fisheries
• Research Institution: Tokyo University of Marine Science and Technology
• Principal Investigator: Hirono Ikuo 東京海洋大学, その他部局等, 教授 (00270926)
• Co-Investigator(Kenkyū-buntansha): KONDO Hidehiro 東京海洋大学, 学術研究院, 准教授 (20314635) ; ITOI Shiro 日本大学, 生物資源科学部, 准教授 (30385992)
• Project Period (FY): 2011-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥43,550,000 (Direct Cost: ¥33,500,000、Indirect Cost: ¥10,050,000); Fiscal Year 2014: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000); Fiscal Year 2013: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000); Fiscal Year 2012: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000); Fiscal Year 2011: ¥23,270,000 (Direct Cost: ¥17,900,000、Indirect Cost: ¥5,370,000)
• Keywords: クルマエビ / WSSV / RNA干渉 / マイクロアレイ / 抗菌タンパク質 / ホワイトスポット病ウイルス / ホワイトスポット病 / 生体防御 / ノックダウン / ウイルス遺伝子ホモログ

Outline of Final Research Achievements

We found that c-type lysozyme and penaeidin like peptide are necessary for normal living of shrimp. In contrast, shrimp can sruvive without i-type lysozyme and crustin in normal environmental condition. We succeeded to develop a kuruma shrimp microarray with about 10,000 unique sequences. We revealed that 2 of WSSV homologues WSSV-H1 and -H2 in shrimp genome have some roles for WSSV infection or replication in shrimp.

Research Products

• [Journal Article] Role of Marsupenaeus japonicus crustin-like peptide against Vibrio penaeicida and white spot syndrome virus infection (2014)
  • Author(s): Sheryll Grospe Hipolito, Aiko Shitara, Hidehiro Kondo, Ikuo Hirono
  • Journal Title: Developmental & Comparative Immunology Volume: 46－2 Issue: 2 Pages: 461-469
  • DOI: 10.1016/j.dci.2014.06.001
  • Peer Reviewed
• [Journal Article] Identification of novel copper/zinc superoxide dismutase (Cu/ZnSOD) genes in kuruma shrimp Marsupenaeus japonicus. (2014)
  • Author(s): Hung MN, Shiomi R, Nozaki R, Kondo H, Hirono I.
  • Journal Title: Fish Shellfish Immunol Volume: 40 Issue: 2 Pages: 472-477
  • DOI: 10.1016/j.fsi.2014.07.030
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Anti-lipopolysaccharide factor isoform 3 from Penaeus monodon (ALFPm3) exhibits antiviral activity by interacting with WSSV structural proteins. (2014)
  • Author(s): Suraprasit S, Methatham T, Jaree P, Phiwsaiya K, Senapin S, Hirono I, Lo CF, Tassanakajon A, Somboonwiwat K.
  • Journal Title: Antiviral Res Volume: 110C Pages: 142-150
  • DOI: 10.1016/j.antiviral.2014.08.005
  • Peer Reviewed
• [Journal Article] Successful yellow head virus infection of Penaeus monodon requires clathrin heavy chain. (2014)
  • Author(s): Posiri P, Kondo H, Hirono I, Panyim S, Ongvarrasopone C.
  • Journal Title: Aquaculture Volume: 435 Pages: 480-487
  • Peer Reviewed
• [Journal Article] New type of heat shock protein 70 homologue gene abounds in the genomic sequence of kuruma shrimp, Marsupenaeus japonicus (2013)
  • Author(s): Aiko Shitara, Hidehiro Kondo, Ikuo Hirono
  • Journal Title: Fisheries Science Volume: 79 Issue: 3 Pages: 397-405
  • DOI: 10.1007/s12562-013-0608-7
  • Peer Reviewed
• [Journal Article] Identification of two penelope-like elements with different structures and chromosome localization in kuruma shrimp genome. (2013)
  • Author(s): Koyama T, Kondo H, Aoki T, Hirono I.
  • Journal Title: Marine Biotechnology Volume: 15 Issue: 1 Pages: 115-123
  • DOI: 10.1007/s10126-012-9474-z
  • Peer Reviewed
• [Journal Article] Molecular mechanisms of the shrimp clotting system. (2012)
  • Author(s): Maningas MB, Kondo H, Hirono I.
  • Journal Title: Fish Shellfish Immunol. Volume: 34 Issue: 4 Pages: 968-972
  • DOI: 10.1016/j.fsi.2012.09.018
  • Peer Reviewed
• [Journal Article] Transglutaminase regulates immune-related genes in shrimp (2012)
  • Author(s): Fagutao FF, Maningas MB, Kondo H, Aoki T, Hirono I
  • Journal Title: Fish Shellfish Immunol Volume: 32 Pages: 711-715
  • Peer Reviewed
• [Journal Article] Uncovering the Mechanisms of Shrimp Innate Immune Response by RNA Interference (2011)
  • Author(s): Hirono I, Fagutao FF, Kondo H, Aoki T
  • Journal Title: Mar Biotechnol Volume: 13 Pages: 622-628
  • Peer Reviewed
• [Journal Article] Microarray Analyses of Shrimp Immune Responses (2011)
  • Author(s): Aoki T, Wang HC, Unajak S, Santos MD, Kondo H, Hirono I
  • Journal Title: Mar Biotechnol Volume: 13 Pages: 629-638
  • Peer Reviewed
• [Journal Article] Molecular characterization and expression analysis of heat shock proteins 40, 70 and 90 from kuruma shrimp Marsupenaeus japonicus (2011)
  • Author(s): Danwattananusrn T, Fagutao FF, Shitara A, Kondo H, A oki T, Nozaki R, Hirono I
  • Journal Title: Fish Sci Volume: 77 Pages: 929-937
  • NAID: 10029654416
  • Peer Reviewed
• [Journal Article] Functional analysis of a C-type lysozyme in Penaeid shrimp (2011)
  • Author(s): Kaizu A, Fagutao FF, Kondo H, Aoki T, Hirono I
  • Journal Title: J Biol.Chem. Volume: 286 Pages: 44344-44349
  • Peer Reviewed
• [Presentation] クルマエビMarsupenaeus japonicus において二本鎖RNAにより誘導される遺伝子について (2014)
  • Author(s): 小松真未・糸井史朗・杉田治男・近藤秀裕・廣野育生
  • Organizer: 平成26年度日本水産学会秋季大会
  • Place of Presentation: 九州大学
  • Year and Date: 2014-09-19 – 2014-09-21
• [Presentation] 次世代シーケンサーを用いたクルマエビゲノムの解析 (2014)
  • Author(s): 設楽愛子・安池元重・中村洋路・藤原篤志・佐野元彦・近藤秀裕・廣野育生
  • Organizer: 平成26年度日本水産学会秋季大会
  • Place of Presentation: 九州大学
  • Year and Date: 2014-09-19 – 2014-09-21
• [Presentation] Molecular markers of fish and shrimp for understanding their immune systems. (2014)
  • Author(s): Shiomi R, Koiwai K, Taechavasonyoo A, Kato G, Nozaki R, Kondo H, and Hirono I.
  • Organizer: Joint 7th AOHUPO Congress/9th PST Symposium
  • Place of Presentation: タイ、バンコク市内ホテル
  • Year and Date: 2014-08-06 – 2014-08-08
  • Invited
• [Presentation] 1.クルマエビMarsupenaeus japonicus の二本鎖RNA応答遺伝子の網羅的発現解析 (2014)
  • Author(s): 小松真未・糸井史朗・杉田治男・近藤秀裕・廣野育生
  • Organizer: 第16回マリンバイオテクノロジー学会大会
  • Place of Presentation: 三重大学
  • Year and Date: 2014-05-31 – 2014-06-01
• [Presentation] 2.クルマエビにおけるIntegrin 分子の血球マーカーへの利用について (2014)
  • Author(s): 塩見玲菜・近藤秀裕・廣野育生
  • Organizer: 第16回マリンバイオテクノロジー学会大会
  • Place of Presentation: 三重大学
  • Year and Date: 2014-05-31 – 2014-06-01
• [Presentation] クルマエビI型リゾチーム遺伝子の発現解析 (2013)
  • Author(s): 西原史晃、井上僚、野崎玲子、近藤秀裕、廣野育生
  • Organizer: 第15回マリンバイテクノロジー学会大会
  • Place of Presentation: 那覇市
• [Presentation] クルマエビ血球マーカー開発のための受容体分子の探索 (2013)
  • Author(s): 小祝敬一郎・野崎玲子・近藤秀裕・廣野育生
  • Organizer: 平成25年度日本水産学会秋季大会
  • Place of Presentation: 三重県津市
• [Presentation] クルマエビにおけるシクロオキシゲナーゼおよびリポキシゲナーゼ遺伝子の同定と発現解析 (2013)
  • Author(s): 塩見玲菜・野崎玲子・近藤秀裕・廣野育生
  • Organizer: 平成25年度日本水産学会秋季大会
  • Place of Presentation: 三重県津市
• [Presentation] IDENTIFICATION AND CHARACTERIZATION OF WSSV HOMOLOGUES IN THE GENOMIC DNA OF KURUMA SHRIMP, Marsupenaeus japonicas (2013)
  • Author(s): Shitara A, Goto K, Koyama T, Kai W, Shigenobu Y, Sugaya T, Sano M, Kondo H, Hirono I
  • Organizer: 第1回国際魚介類免疫学会(1st International Conference of Fish and Shellfish Immunology)
  • Place of Presentation: スペイン、Vigo
• [Presentation] ROLE OF Marsupenaeus japonicus CRUSTIN-LIKE PEPTIDE AGAINST Vibrio penaeicida AND WHITE SPOT VIRUS INFECTION (2013)
  • Author(s): Hipolito SG, Kondo H, Hirono I
  • Organizer: 第1回国際魚介類免疫学会(1st International Conference of Fish and Shellfish Immunology)
  • Place of Presentation: スペイン、Vigo
• [Presentation] IDENTIFICATION OF RECEPTOR MOLECULES FOR THE DEVELOPMENT OF HAEMOCYTE CLASSIFICATION MARKER IN KURUMA PRAWN Marsupenaeus japonicus (2013)
  • Author(s): Keiichiro Koiwai, Reiko Nozaki, Hidehiro Kondo and Ikuo Hirono
  • Organizer: 第3回国際水産学シンポジウム（International Fisheries Symposium 2013）
  • Place of Presentation: タイ、パタヤ
• [Presentation] Role of Marsupenaeus japonicus crustin-like peptide against Vibrio penaeicida and white spot virus infection (2012)
  • Author(s): Sheryll G. Hipolito, Hidehiro Kondo, Ikuo Hirono,
  • Organizer: 国際比較免疫学会2012
  • Place of Presentation: 福岡
• [Presentation] Identification of WSSV homologues in the genomic DNA of kuruma shrimp, Marsupenaeus japonicus (2012)
  • Author(s): Aiko Shitara, Takashi Koyama, Kana Goto, Yuya Shigenobu, Takuma Sugaya, Motohiko Sano, Hidehiro Kondo, Ikuo Hirono
  • Organizer: アジア太平洋マリンバイオテクノロジー会議2012
  • Place of Presentation: 高知
• [Presentation] Non-specific immune activation against double stranded RNA in kuruma shrimp, Marsupenaeus japonicus (2012)
  • Author(s): Ryo Inoue, Hidehiro Kondo, Ikuo Hirono
  • Organizer: アジア太平洋マリンバイオテクノロジー会議2012
  • Place of Presentation: 高知
• [Presentation] Antimicrobial proteins of shrimp (2012)
  • Author(s): Hirono I
  • Organizer: アジア太平洋マリンバイオテクノロジー会議2012
  • Place of Presentation: 高知
  • Invited
• [Presentation] Recent advances in shrimp immunology and genome science (2012)
  • Author(s): Ikuo Hirono
  • Organizer: 2012 Taiwan-Japan Symposium on Prospective Biotechnology for Aquaculture
  • Place of Presentation: 台湾(台南)(招待講演)
• [Presentation] クルマエビの二本鎖RNAに対する非特異的生体防御機構に関する研究 (2012)
  • Author(s): 井上僚、近藤秀裕、廣野育生
  • Organizer: 平成24年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学(東京)
• [Presentation] クルマエビゲノム配列解析によるWSSV類似遺伝子の探索 (2012)
  • Author(s): 設楽愛子、重信裕弥、菅谷琢磨、佐野元彦、近藤秀裕、廣野育生
  • Organizer: 平成24年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学(東京)
• [Presentation] Molecular immunology and genomic science of shrimp (2011)
  • Author(s): Ikuo Hirono
  • Organizer: 8th Symposium of Diseases in Asian Aquaculture
  • Place of Presentation: インド(マンガロール)(招待講演)
• [Presentation] Shrimp genome science and immunology (2011)
  • Author(s): Ikuo Hirono
  • Organizer: 38th Annual Convention of the Philippine Society of Biochemistry and Molecular Biology
  • Place of Presentation: フィリピン(マニラ)(招待講演)
• [Presentation] Identification of 22 genes homologous to WSSV genome in the genomic DNA of kuruma shrimp, Marsupenaeus japonicus (2011)
  • Author(s): Shitara A, Yamada H, Kondo H, Hirono I
  • Organizer: The Crustacean Society Summer Meeting 2011
  • Place of Presentation: ハワイ(ホノルル)
• [Presentation] Functional analysis of WSSV genes homologues in kuruma shrimp, Marsupenaeus japonicas (2011)
  • Author(s): H Yamada, A Shitara, A Kaizu, MBB Maningas, H Kondo, I Hirono
  • Organizer: The Crustacean Society Summer Meeting 2011
  • Place of Presentation: ハワイ(ホノルル)
• [Presentation] Cloning and gene expression of invertebrate type lysozyme from Kuruma shrimp, Marsupenaeus japonicas (2011)
  • Author(s): R.Inoue, T.Nagayoshi, H.Kondo, I.Hirono, A.Tassanakajon
  • Organizer: The Crustacean Society Summer Meeting 2011
  • Place of Presentation: ハワイ(ホノルル)
• [Presentation] Transcriptome analysis of fish and shellfish for development of prevention method against microbial pathogens
  • Author(s): Hidehiro Kondo and Ikuo Hirono
  • Organizer: 国際水圏メタゲノムシンポジウム「水圏メタゲノミクスの展開と水圏生物多様性研究の展望」
  • Place of Presentation: 北里大学薬学部コンベンションホール
  • Invited