| Budget Amount *help |
¥49,660,000 (Direct Cost: ¥38,200,000、Indirect Cost: ¥11,460,000)
Fiscal Year 2015: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
Fiscal Year 2014: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
Fiscal Year 2013: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
Fiscal Year 2012: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
Fiscal Year 2011: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
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| Outline of Final Research Achievements |
One interesting application of nuclear transfer (NT) techniques is the resurrection of extinct species and the rescue of endangered species. However, current success rate for producing live animals by cloning is very low. Therefore, we tried to improve nuclear transfer procedure one by one, and applied this techniques to generate cloned mice from frozen cadaver or fur, or cells collected from excrement or urine. By using most recent nuclear transfer techniques, we could succeeded to generate cloned mice from somatic cells of cloned mice, and probably this process can repeat unlimitedly because we could not find any accumulation of abnormality in those re-cloned mice. On the other hand, we also succeeded to generate cloned mice from urine-derived cells. This suggest that cells derived from urine, which can be collected noninvasively, may be used in the rescue of endangered mammalian species by using nuclear transfer without causing injury to the animal.
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