|Budget Amount *help
¥47,320,000 (Direct Cost: ¥36,400,000、Indirect Cost: ¥10,920,000)
Fiscal Year 2014: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
Fiscal Year 2013: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
Fiscal Year 2012: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000)
Fiscal Year 2011: ¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
|Outline of Final Research Achievements
We established PrPSc-specific staining method and using this method we elucidated a part of neuropathophysiology in the early stage of prion infection; astrocytes recognize prion propagation directly or subtle changes in neurons by prion propagation and astrocyte activation precedes over microglial activation.
Gene expression analysis of microglia in prion-infected mice showed that microglia play a neuroprotective role by secreting neurotrophic factors at the early stage of prion infection in the early stage of prion infection, but the microglial activation state shifts to pro-inflammatory or neurotoxic state along with the progression of prion disease. Furthermore, anti-inflammatory cytokines such as IL-10 interfere the propagation of prions in brain.