| Co-Investigator(Kenkyū-buntansha) |
SHINANO Takuro 独立行政法人農業・食品産業技術総合研究機構, 東北農業研究センター, 農業放射線研究センター長 (20235542)
IKEDA Seishi 独立行政法人農業・食品産業技術総合研究機構, 北海道農業研究センター, 主任研究員 (20396609)
YAMASHITA Akifumi 東北大学, 大学院生命科学研究科, 助教 (00573180)
IKEDA Seishi 独立行政法人農業・食品産業技術総合研究機構, 北海道農業研究センター, 研究員 (40414750)
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| Budget Amount *help |
¥50,700,000 (Direct Cost: ¥39,000,000、Indirect Cost: ¥11,700,000)
Fiscal Year 2013: ¥13,130,000 (Direct Cost: ¥10,100,000、Indirect Cost: ¥3,030,000)
Fiscal Year 2012: ¥13,130,000 (Direct Cost: ¥10,100,000、Indirect Cost: ¥3,030,000)
Fiscal Year 2011: ¥24,440,000 (Direct Cost: ¥18,800,000、Indirect Cost: ¥5,640,000)
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| Research Abstract |
We investigated bacterial communities in paddy rice with low (LN), standard (SN) levels of N fertilizer application. Metagenome analysis suggested shifts of bacterial communities between LN and SN root microbiomes. The abundances of functional genes for methane oxidation were significantly increased in LN root microbiome. Plants have mutualistic symbiotic relationships with microorganisms by CCaMK. We examined the effect of an OsCCaMK mutant (NE1115). The CH4 flux of NE1115 in the LN field was significantly higher than that of WT, although no difference was observed in the SN field. qPCR and 13C/15N isotopic determinations suggested that CH4 oxidation and N2 fixation were simultaneously activated in the root of WT rice in the LN field, and both processes are likely controlled by OsCCaMK. Metaproteome and CARD-FISH analyses demonstrated that both processes are mediated by type II methanotrophs inhabiting the vascular bundles and epidermal cells of the rice roots.
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