Integrated molecular analysis of taste signal generation, transmission and cognition
| Project/Area Number |
23248058
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
ABE Keiko 東京大学, 農学生命科学研究科, 特任教授 (10151094)
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| Co-Investigator(Kenkyū-buntansha) |
AIZAWA Hiroyuki 東京大学, 大学院農学生命科学研究科, 特任准教授 (90221704)
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| Co-Investigator(Renkei-kenkyūsha) |
ISHIMARU Yoshiro 東京大学, 大学院農学生命科学研究科, 特任准教授 (10451840)
OKADA Shinji 東京大学, 大学院農学生命科学研究科, 特任准教授 (50376563)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥50,830,000 (Direct Cost: ¥39,100,000、Indirect Cost: ¥11,730,000)
Fiscal Year 2013: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
Fiscal Year 2012: ¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000)
Fiscal Year 2011: ¥26,520,000 (Direct Cost: ¥20,400,000、Indirect Cost: ¥6,120,000)
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| Keywords | 食品 / 味覚 / 脳・神経 / シグナル伝達 / 細胞・組織 |
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| Research Abstract |
Outline of the research: For the integrated analysis of taste systems, (1) we found the target genes AnoI, Kene3, Sec61a1 that are involved in construction of electric potential of saltiness reception (type I) cell membrane; (2) PLC-beta2-WGA medaka fish analysis led to comprehensive elucidation of the taste transmission pathway from bitterness/amino acid receiving cells to the endbrain and the visualization of saltiness-receiving cell to the NST taste area in PKD1L3-WGA mice was successive; and (3) integrated expression markers in weaning mice, SNAP25, EGr-2 and Krox-20, were distinctly activated its signal in the taste area when the mice were given a solid food at the stage of weaning in comparison with the case that they were still given a liquid food. (4) we also showed that there is a relationship between the tastant quality in diet and the food intake.
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Report
(4 results)
Research Products
(112 results)
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[Journal Article] Human sweet taste receptor mediates acid- induced sweetness of miraculin、Proc.2011
Author(s)
Koizumi, A, Tsuchiya A, Nakajima K, Ito K, Terada T, Shimizu-Ibuka A, Loic, B, Asakura T, Takumi M, Abe k
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Journal Title
Natl. Acad. Sci.
Volume: 108
Issue: 40
Pages: 16819-16824
DOI
Related Report
Peer Reviewed
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[Journal Article] Non-Acidic Compounds Induce the Intense Sweet Taste of Neoculin, a Taste-Modifying Protein2011
Author(s)
Nakajima, K, Koizumi A, Iizuka K, Ito K, Morita Y, Koizumi T, Asakura T, Shimizu-Ibuka A, Misaka T, Abe K
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Journal Title
Bioscience, Biotechnology, and Biochemistry
Volume: 75
Issue: 8
Pages: 1600-1602
DOI
NAID
ISSN
0916-8451, 1347-6947
Related Report
Peer Reviewed
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[Journal Article] Cysteine-to-serine shuffling using a Saccharomyces cerevisiae expression system improves protein secretion : case of a nonglycosylated mutant of miraculin, a taste-modifying protein2011
Author(s)
Ito, K., T.Sugawara, A.Koizumi, K.Nakajima, A.Shimizu-Ibuka, M.Shiroishi, H.Asada, T.Yurugi-Kobayashi, T.Shimamura, T.Asakura, T.Misaka, S.Iwata, T.Kobayashi, K.Abe
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Journal Title
Biotechnology Letters
Volume: Vol.33
Issue: 1
Pages: 103-107
DOI
NAID
Related Report
Peer Reviewed
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