The mechanistic basis of human epigenome establishement
Project/Area Number |
23249019
|
Research Category |
Grant-in-Aid for Scientific Research (A)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Human genetics
|
Research Institution | Kyushu University |
Principal Investigator |
SASAKI Hiroyuki 九州大学, 生体防御医学研究所, 教授 (30183825)
|
Co-Investigator(Renkei-kenkyūsha) |
ICHIYANAGI Kenji 九州大学, 生体防御医学研究所, 助教 (70401560)
|
Project Period (FY) |
2011-05-31 – 2014-03-31
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥48,360,000 (Direct Cost: ¥37,200,000、Indirect Cost: ¥11,160,000)
Fiscal Year 2013: ¥15,340,000 (Direct Cost: ¥11,800,000、Indirect Cost: ¥3,540,000)
Fiscal Year 2012: ¥15,340,000 (Direct Cost: ¥11,800,000、Indirect Cost: ¥3,540,000)
Fiscal Year 2011: ¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
|
Keywords | 遺伝学 / エピゲノム / DNAメチル化 / 小分子RNA |
Research Abstract |
In tis study, we tried to identify factors that determine DNA methylation patterns of human cells, with a long-term goal of clarifying mechanisms regulating gene expression. First, we identified ZBTB24 protein as a novel methylation pattern determinant, based on the study of an immune-deficiency syndrome accompanied by DNA hypomethylation at certain DNA sequences. A gene knockout study in mice confirmed that it is indeed a factor regulating DNA methylation. Second, based on the comparative study of methylation patterns between human and chimpanzee, we identified CTCF protein, a transcription factor, and its target sequence as methylation determinants. Species-specific sequence changes that disrupted CTCF binding were correlated with hypermethylation. Lastly, we identified CpG dinucleotide density in tandem repetitive sequences as another determinant. Our results provide a basis to study the roles of methylation in evolution, variability and disease such as cancer.
|
Report
(4 results)
Research Products
(23 results)
-
[Journal Article] Three novel ZBTB24 mutations identified in Japanese and Cape Verdean type 2 ICF syndrome patients2013
Author(s)
Nitta, H., Unoki, M., Ichiyanagi, K., Kosho, T., Shigemura, T., Takahashi, H., Velasco, G., Francastel, C., Picard, C., Kubota, T. & Sasaki, H.
-
Journal Title
J. Hum. Genet.
Volume: 58
Issue: 7
Pages: 455-460
DOI
NAID
Related Report
Peer Reviewed
-
-
-
-
-
[Presentation] 2型ICF症候群原因遺伝子ZBTB24のDNAメチル化制御機構の解明2013
Author(s)
新田洋久,鵜木元香,一柳健司,古庄知己,重村倫成,高橋浩士, Guillaume Velasco, Claire Francastel, CapucinePicard,大津真,金子新,久保田健夫,佐々木裕之
Organizer
日本人類遺伝学会第58回大会
Place of Presentation
仙台
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-