Using proteomics to elucidate pathogenesis and establish new diagnostic or treatment evaluation methods in refractory gastrointestinal diseases
Project/Area Number |
23249043
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Research Category |
Grant-in-Aid for Scientific Research (A)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Gastroenterology
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Research Institution | Kagoshima University |
Principal Investigator |
TSUBOUCHI Hirohito 鹿児島大学, 医歯(薬)学総合研究科, 客員研究員 (60145480)
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Co-Investigator(Kenkyū-buntansha) |
IDO Akio 鹿児島大学, 大学院・医歯学総合研究科, 教授 (30291545)
OKETANI Makoto 鹿児島大学, 医学部・歯学部附属病院, 講師 (50274816)
UTO Hirofumi 鹿児島大学, 大学院・医歯学総合研究科, 講師 (20347058)
|
Project Period (FY) |
2011-04-01 – 2014-03-31
|
Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥47,840,000 (Direct Cost: ¥36,800,000、Indirect Cost: ¥11,040,000)
Fiscal Year 2013: ¥13,520,000 (Direct Cost: ¥10,400,000、Indirect Cost: ¥3,120,000)
Fiscal Year 2012: ¥10,790,000 (Direct Cost: ¥8,300,000、Indirect Cost: ¥2,490,000)
Fiscal Year 2011: ¥23,530,000 (Direct Cost: ¥18,100,000、Indirect Cost: ¥5,430,000)
|
Keywords | プロテオミクス / C型慢性肝炎 / HCV / AIM / 補体C4断片 / ヒト好中球ペプチド / 炎症性腸疾患 / NAFLD / 膵炎 / 肝細胞癌 / 非アルコール性脂肪肝炎 / 膵癌 / 自己免疫性膵炎 / 潰瘍性大腸炎 / クローン病 |
Research Abstract |
We identified several serum proteins present at high levels in patients with liver disease or inflammatory bowel disease, including complement component C4 fragment (C4-F), kininogen fragment (Kini-F), apoptosis inhibitor (AIM), and human neutrophil peptide (HNP)-1. Serum C4-F and Kini-F are potentially biomakers for antiviral effects in patients with chronic hepatitis C or diagnosis of nonalcoholic fatty liver disease (NAFLD), respectively. In patients with chronic liver disease, serum AIM levels were associated with liver fibrosis. AIM also appeared to contribute to the pathogenesis of NAFLD. Furthermore, HNP-1 worsened pathological condition in the mouse model of NAFLD and alcoholic liver disease, in addition to ulcerative colitis. Thus, proteomics is a useful tool for identifying proteins that might serve as candidate biomarkers in refractory gastrointestinal diseases, and the identified proteins may be involve in the pathological conditions associated with those diseases.
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Report
(4 results)
Research Products
(42 results)
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[Journal Article] Serum levels of apoptosis inhibitor of macrophage are associated with hepatic fibrosis in patients with chronic hepatitis C2014
Author(s)
Mera K, Uto H, Mawatari S, Ido A, Yoshimine Y, Nosaki T, Oda K, Tabu K, Kumagai K, Tamai T, Moriuchi A, Oketani M, Shimada Y, Hidaka M, Eguchi S, Tsubouchi H
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Journal Title
BMC Gastroenterol
Volume: 14
Issue: 1
Pages: 27-27
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] Hepatitis C virus NS3/4A protease inhibits complement activation by cleaving complement component 42013
Author(s)
Mawatari S, Uto H, Ido A, Nakashima K, Suzuki T, Kanmura S, Kumagai K, Oda K, Tabu K, Tamai T, Moriuchi A, Oketani M, Shimada Y, Sudoh M, Shoji I, Tsubouchi H
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Journal Title
PLoS One
Volume: 8(12)
Issue: 12
Pages: e82094-e82094
DOI
Related Report
Peer Reviewed
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[Journal Article] Ibusuki R, Uto H, Arima S, Mawatari S, Setoguchi Y, Iwashita Y, Hashimoto S, Maeda T, Tanoue S, Kanmura S, Oketani M, Ido A, Tsubouchi H2013
Author(s)
Ibusuki R, Uto H, Arima S, Mawatari S, Setoguchi Y, Iwashita Y, Hashimoto S, Maeda T, Tanoue S, Kanmura S, Oketani M, Ido A, Tsubouchi H
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Journal Title
L-amino acid-defined diet. Liver Int
Volume: 33(10)
Issue: 10
Pages: 1549-56
DOI
Related Report
Peer Reviewed
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[Journal Article] Correlation of serum levels of complement C4a desArg with pathologically estimated severity of glomerular lesions and mesangial hypercellularity scores in patients with IgA nephropathy2013
Author(s)
Sogabe A, Uto H, Kanmura S, Nosaki T, Oyamada M, Tokunaga K, Nishida C, Fukumoto M, Oku M, Nishimoto K, Takenouchi S, Ido A, Shimada Y, Tsubouchi H
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Journal Title
Int J Mol Med
Volume: 32(2)
Issue: 2
Pages: 307-14
DOI
Related Report
Peer Reviewed
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[Journal Article] Hepatocyte growth factor stimulates the migration of gastric epithelial cells by altering the subcellular localization of the tight junction protein ZO-12013
Author(s)
Nasu Y, Ido A, Tanoue S, Hashimoto S, Sasaki F, Kanmura S, Setoyama H, Numata M, Funakawa K, Moriuchi A, Fujita H, Sakiyama T, Uto H, Oketani M, Tsubouchi H
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Journal Title
J Gastroenterol
Volume: 48
Issue: 2
Pages: 193-202
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] Difference in serum complement component C4a levels between hepatitis C virus carriers with persistently normal alanine aminotransferase levels or chronic hepatitis C2012
Author(s)
Imakiire K, Uto H, Sato Y, Sasaki F, Mawatari S, Ido A, Shimoda K, Hayashi K, Stuver SO, Ito Y, Okanoue T, Tsubouchi H
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Journal Title
Mol Med Rep
Volume: 6(2)
Pages: 259-64
DOI
Related Report
Peer Reviewed
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[Journal Article] High serum thioredoxin levels are reduced after tonsillectomy in patients with IgA nephropathy2012
Author(s)
Nosaki T, Uto H, Takami Y, Oku M, Fukumoto M, Mera K, Nishida C, Tokunaga K, Sogabe A, Oketani M, Ido A, Kurono Y, Tsubouchi H
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Journal Title
Intern Med
Volume: 51(6)
Pages: 559-65
NAID
Related Report
Peer Reviewed
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[Journal Article] Human neutrophil peptide-1 aggravates dextran sulfate sodium-induced colitis2012
Author(s)
Hashimoto S, Uto H, Kanmura S, Sakiyama T, Oku M, Iwashita Y, Ibusuki R, Sasaki F, Ibusuki K, Takami Y, Moriuchi A, Oketani M, Ido A, Tsubouchi H
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Journal Title
Inflamm Bowel Dis
Volume: 18(4)
Issue: 4
Pages: 667-75
DOI
Related Report
Peer Reviewed
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[Journal Article] Serum manganese superoxide dismutase and thioredoxin are potential prognostic markers for hepatitis C virus-related hepatocellular carcinoma2011
Author(s)
Tamai T, Uto H, Takami Y, Oda K, Saishoji A, Hashiguchi M, Kumagai K, Kure T, Mawatari S, Moriuchi A, Oketani M, Ido A, Tsubouchi H
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Journal Title
World J Gastroenterol
Volume: 17 (44)
Issue: 44
Pages: 4890-8
DOI
Related Report
Peer Reviewed
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[Journal Article] Sorafenib and hepatic arterial infusion chemotherapy for unresectable advanced hepatocellular carcinoma : A comparative study2011
Author(s)
Hiramine Y, Uto H, Imamura Y, Tabu K, Baba Y, Hiwaki T, Sho Y, Tahara K, Higashi H, Tamai T, Oketani M, Ido A, Tsubouchi H
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Journal Title
Exp Ther Med
Volume: 2(3)
Issue: 3
Pages: 433-441
DOI
Related Report
Peer Reviewed
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[Presentation] Hepatic fibrosis and apoptosis induced by ethanol administration are exacerbated in human neutrophil peptide-1 transgenic mice2014
Author(s)
Ibusuki R, Uto H, Kozono M, Oda K, Ohshige A, Kumagai K, Mawatari S, Tamai T, Moriuchi A, Oketani M, Ido A, Tsubouchi H
Organizer
49th the international liver congress 2014 of the European Association for the Study of Liver Diseases (EASL)
Place of Presentation
London (UK)
Year and Date
2014-04-10
Related Report
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[Presentation] Hepatic fibrosis and apoptosis induced by ethanol administration are exacerbated in human neutrophil peptide-1 transgenic mice2014
Author(s)
Rie Ibusuki, Hirofumi Uto, Masaya Kozono, Kohei Oda, Akihiko Ohshige, Kotaro Kumagai, Seiichi Mawatari, Tsutomu Tamai, Akihiro Moriuchi, Makoto Oketani, Akio Ido, HirohitoTsubouchi.
Organizer
European Association for the Study of the Liver (EASL)
Place of Presentation
London, UK
Related Report
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[Presentation] The association between serum levels of apoptosis inhibitor of macrophage and the clinical features of nonalcoholic fatty liver disease2013
Author(s)
Oda K, Uto H, Mawatari S, Tabu K, Ohno K, Toyokura E, Oshige A, Imanaka D, Kumagai K, Tamai T, Moriuchi A, Oketani M, Ido A, Sumida Y, Tsubouchi H
Organizer
The 64th annual meeting of the American Association for the Study of Liver Diseases (AASLD)
Place of Presentation
Washington DC (USA)
Year and Date
2013-11-02
Related Report
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[Presentation] Hepatitis C virus NS3/4A protease inhibits complement activation through cleavage of complement component C42012
Author(s)
Mawatari S, Uto H, Kumagai K, Oda K, Tabu K, Ohno K, Oshige A, Imanaka D, Tamai T, Moriuchi A, Oketani M, Ido A, Tsubouchi H, Sato Y, Suzuki T
Organizer
The 63th annual meeting of the AASLD
Place of Presentation
Boston(USA)
Year and Date
2012-11-11
Related Report
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[Presentation] Serum levels of apoptosis inhibitor of macrophage (AIM) are associated with hepatic fibrosis and insulin resistance in patients with hepatitis C virus-associated liver disease2012
Author(s)
Mera K, Uto H, Sato Y, Yoshimine Y, Oda K, Imanaka D, Saishoji A, Tamai T, Nosaki T, Moriuchi A, Oketani M, Ido A, Tsubouchi H
Organizer
Digestive Disease Week (DDW)
Place of Presentation
Sandiego (USA)
Year and Date
2012-05-19
Related Report
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[Presentation] Human neutrophil peptide-1 aggravates hepatic fibrosis in a nonalcoholic steatohepatitis mouse model2011
Author(s)
Ibusuki R, Uto H, Arima S, Mawatari S, Iwashita Y, Hashimoto S, Maeda T, Tamai T, Moriuchi A, Setoguchi Y, Oketani M, Ido A, Tsubouchi H
Organizer
The 62th annual meeting of the AASLD
Place of Presentation
San Francisco (USA)
Year and Date
2011-11-05
Related Report
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