• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Investigation of a novel technique for regeneration of elastic fibers with three-dimensional culture on a collagen sponge scaffold and the addition of latent TGF-beta binding protein 4

Research Project

Project/Area Number 23249078
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Plastic surgery
Research InstitutionKyoto University

Principal Investigator

Suzuki Shigehiko  京都大学, 医学(系)研究科(研究院), 教授 (30187728)

Co-Investigator(Kenkyū-buntansha) NAITOH Motoko  京都大学, 大学院・医学研究科, 講師 (30378723)
KAWAI Katsuya  京都大学, 大学院・医学研究科, 准教授 (90273458)
Co-Investigator(Renkei-kenkyūsha) NAKAMURA Tomoyuki  関西医科大学, 医学部, 教授 (20362527)
Project Period (FY) 2011-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥31,200,000 (Direct Cost: ¥24,000,000、Indirect Cost: ¥7,200,000)
Fiscal Year 2015: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2012: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2011: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
Keywords人工皮膚 / LTBP-4 / 弾性線維 / コラーゲン / コラーゲンスポンジ / 再生医療 / スカフォールド / エラスチン / 再生医学 / 弾性線維再生 / 機能性高分子 / 三次元培養 / 架橋条件 / 基材
Outline of Final Research Achievements

The objective of this study was to investigate the effects of latent TGF-β binding protein 4 (LTBP-4) on elastic fiber regeneration in three-dimensional cultures of human dermal fibroblasts (HDFs). Collagen sponges cross-linked at 120 °C and composed of small pores (25 μm on average) was favorable for elastic fiber regeneration by HDFs. Addition of LTBP-4, followed by culture for 21 days, accelerated elastic fiber accumulation within the scaffolds. Conditioned scaffolds containing either HDFs or LTBP-4-built mature elastic fibers were implanted between the dermis and the cutaneous muscle of mice. The combined use of HDFs and LTBP-4 resulted in thicker tissues containing elastic fibers. These results indicate that weakly cross-linked collagen sponges can be used as scaffolds for regenerating elastic fibers both in vitro and in vivo, and that the addition of LTBP-4 accelerates the deposition of both elastin and fibrillin-1, and increases cell proliferation.

Report

(6 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (14 results)

All 2016 2015 2014 2013 2012 2011

All Journal Article (4 results) (of which Peer Reviewed: 3 results,  Open Access: 1 results) Presentation (8 results) Book (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Regeneration of elastic fibers by three-dimensional culture on a collagen scaffold and the addition of latent TGF-β binding protein 4 to improve elastic matrix deposition.2015

    • Author(s)
      Aya R, Ishiko T, Noda K, Yamawaki S, Sakamoto Y, Tomihata K, Katayama Y, Yoshikawa K, Kubota H, Nakamura T, Naitoh M, Suzuki S
    • Journal Title

      Biomaterials

      Volume: 72 Pages: 29-37

    • DOI

      10.1016/j.biomaterials.2015.08.036

    • NAID

      120005753831

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A case of giant naevus followed up for 22 years after treatment with artificial dermis.2013

    • Author(s)
      Suzuki S., Morimoto N., Yamawaki S., Fujitaka J., Kawai K.
    • Journal Title

      J Plast Reconstr Aesthet Surg,

      Volume: 66 Issue: 8 Pages: e229-e233

    • DOI

      10.1016/j.bjps.2013.03.018

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Latent TGF-beta binding protein 4 promotes elastic fiber assembly by interacting with fibulin-5.2013

    • Author(s)
      Noda K., Dabovic B., Takagi K., Inoue T., Horiguchi M., Hirai M., Fujikawa Y., Akama T. O., Kusumoto K., Zilberberg L., Sakai L. Y., Koli K., Naitoh M., von Melchner H., Suzuki S., Rifkin D. B., Nakamura T
    • Journal Title

      Proceedings of the National Academy of Sciences of the United States of America

      Volume: 110 Issue: 8 Pages: 2852-2857

    • DOI

      10.1073/pnas.1215779110

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 創傷治癒2012

    • Author(s)
      内藤素子,鈴木茂彦
    • Journal Title

      再生医療

      Volume: 11 Pages: 12-13

    • NAID

      40019321389

    • Related Report
      2012 Annual Research Report
  • [Presentation] ヒト線維芽細胞とLTBP-4を用いた 弾性線維の再生2016

    • Author(s)
      綾梨乃、内藤素子、石河利広、山脇聖子、片山泰博、野田和男、坂元悠紀、中邨智之、 鈴木茂彦
    • Organizer
      第15回日本再生医療学会総会
    • Place of Presentation
      大阪
    • Year and Date
      2016-03-17
    • Related Report
      2015 Annual Research Report
  • [Presentation] 形成外科(創傷外科学分野)において臨床応用されているバイオマテリアル2015

    • Author(s)
      鈴木茂彦
    • Organizer
      第37回バイオマテリアル学会大会
    • Place of Presentation
      京都
    • Year and Date
      2015-11-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] 線維芽細胞の三次元培養におけるLTBP-4の弾性線維再生促進作用2014

    • Author(s)
      綾梨乃, 石河利広, 内藤素子, 山脇聖子, 片山泰博, 坂元悠紀, 中邨智之, 鈴木茂彦
    • Organizer
      第23回 日本形成外科学会基礎学術集会
    • Place of Presentation
      松本
    • Year and Date
      2014-10-09
    • Related Report
      2014 Annual Research Report
  • [Presentation] 線維芽細胞の三次元培養においてLTBP-4を添加すると弾性線維の形成が促進される2013

    • Author(s)
      綾梨乃,石河利広,内藤素子,野田和男,山脇聖子,吉川勝宇,坂本悠紀,中邨智之,鈴木茂彦
    • Organizer
      第22回日本形成外科学会基礎学術集会
    • Place of Presentation
      新潟朱鷺メッセ
    • Year and Date
      2013-11-07
    • Related Report
      2013 Annual Research Report
  • [Presentation] Latent TGFβ binding protein 4 (LTBP-4)はfibulin-5との相互作用を介して弾性線維形成を促進する2013

    • Author(s)
      野田和男, 高木恭子, 井上唯史, 藤川雄介, 赤間智也, 内藤素子, 鈴木茂彦
    • Organizer
      第34回 日本炎症・再生医学
    • Place of Presentation
      京都国際会館
    • Year and Date
      2013-07-02 – 2013-07-03
    • Related Report
      2013 Annual Research Report
  • [Presentation] 線維芽細胞の三次元培養による弾性線維の再生2012

    • Author(s)
      綾 梨乃、石河利広、内藤素子、山脇聖子、吉川勝宇、坂本悠紀、鈴木茂彦
    • Organizer
      第21回日本形成外科学会基礎学術集会
    • Place of Presentation
      ホテルリステル猪苗代
    • Related Report
      2012 Annual Research Report
  • [Presentation] Alternative Techniques in Wound Management―Usefulness of Our Artificial Dermis, Pelnac and Its Further Development.2011

    • Author(s)
      Suzuki S.
    • Organizer
      The 8th Asia- Pacific Burn Congress & The 3rd Congress of the Asian Wound Healing Association.
    • Place of Presentation
      Bangkok
    • Related Report
      2011 Annual Research Report
  • [Presentation] 瘢痕・瘢痕拘縮治療の基本的考え方2011

    • Author(s)
      鈴木茂彦
    • Organizer
      日本創傷外科学会
    • Place of Presentation
      ホテルルネッサンス札幌
    • Related Report
      2011 Annual Research Report
  • [Book] 熱傷治療マニュアル2013

    • Author(s)
      鈴木茂彦、河合勝也
    • Total Pages
      476
    • Publisher
      中外医学社
    • Related Report
      2013 Annual Research Report
  • [Patent(Industrial Property Rights)] 三次元培養弾性線維組織の製造方法2012

    • Inventor(s)
      鈴木茂彦,内藤素子,石河利広,綾梨乃,平嗣良
    • Industrial Property Rights Holder
      鈴木茂彦,内藤素子,石河利広,綾梨乃,平嗣良
    • Industrial Property Rights Type
      特許
    • Filing Date
      2012-11-14
    • Related Report
      2012 Annual Research Report

URL: 

Published: 2011-04-06   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi