High sensitive fluorometric biosniffer based on UV-LED emission light and metabolizing enzyme
Project/Area Number |
23300068
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Perception information processing/Intelligent robotics
|
Research Institution | Tokyo Medical and Dental University |
Principal Investigator |
|
Co-Investigator(Kenkyū-buntansha) |
KUDO Hiroyuki 東京医科歯科大学, 生体材料工学研究所, 准教授 (70329118)
ARAKA WA Takahiro 東京医科歯科大学, 生体材料工学研究所, 講師 (50409637)
|
Project Period (FY) |
2011-04-01 – 2014-03-31
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥20,280,000 (Direct Cost: ¥15,600,000、Indirect Cost: ¥4,680,000)
Fiscal Year 2013: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2012: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2011: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
|
Keywords | UV-LED / 匂い / 高感度 / 選択性 / 代謝酵素 / 蛍光 / 揮発成分 / 蛍光法 / 電子受容体 |
Research Abstract |
A fluorometric biochemical gas sensor (biosniffer) for odor chemicals was developed by incorporating with an UV-LED emission light, a novel polymer PMEH (with low auto fluorescence) and metabolizing enzymes. The gas sensor immobilized formaldehyde dehydrogenase can successfully measure gaseous formaldehyde (lower detection limit: 750 ppt [parts per trillion]; human smell detection limit: 400 ppb [parts per billion]) with high gas selectivity. The sensor system was applicable for monitoring a different volatile chemical by changing it with other dehydrogenase enzyme.
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Report
(4 results)
Research Products
(12 results)