| Budget Amount *help |
¥20,280,000 (Direct Cost: ¥15,600,000、Indirect Cost: ¥4,680,000)
Fiscal Year 2013: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2012: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2011: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
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| Research Abstract |
In order to construct a stabilized and synchronized synthetic genetic oscillator, this research was carried out. At first, a micro fluid reactor was produced for keeping the extracellular condition constant. We used this reactor and reported plasmids for GFP oscillation experiment. As the result of this experiment, we found there were three big problems. 1) The amplitude and the period of the oscillation for each cell was wildly varied. For statistical analysis, the large numbers of data are needed. 2) We needed a long time for image analysis because there were no automatic analysis software. 3) We had many troubles in micro fluid reactor because the cells were piled up and were moved in the reactor. For the further experiment, the modifications of the micro fluid reactor and the automatic image analysis software are needed.
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