Establishment of evolutional microscopy on neurons with ultra-fast two-photon microscope
| Project/Area Number |
23300121
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neuroscience in general
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| Research Institution | Waseda University |
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Principal Investigator |
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥20,020,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥4,620,000)
Fiscal Year 2013: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2012: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2011: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
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| Keywords | 分子動態 / タンパク質 / 神経細胞 / シナプス / 活動電位 / 膜電位計測 / 樹状突起 / スパイン / ２光子励起顕微鏡 / 蛍光相関分光法 / 拡散 / 2光子励起顕微鏡 / 分子・細胞神経科学 / タンパク質の動態 |
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| Research Abstract |
This research project aimed to measure dynamics of protein molecules rapidly moving in the cytosol of neuronal dendrites at multiple points simultaneously by means of a newly developed two-photon microscope. I succeeded in recording at multiple points at more than 10 kHz of time resolution from cytosol of primary cultured neurons derived from mouse, in which GFP or GFP-fused CamKII proteins were expressed, and in analyzing by FCS. I was also successful in recording action potentials from multiple cultured neurons, which enabled to analyze synaptic connections between neuron pairs and also direction of synaptic connections.
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Report
(4 results)
Research Products
(15 results)
