| Budget Amount *help |
¥20,020,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥4,620,000)
Fiscal Year 2013: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2012: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2011: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
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| Research Abstract |
This research project aimed to measure dynamics of protein molecules rapidly moving in the cytosol of neuronal dendrites at multiple points simultaneously by means of a newly developed two-photon microscope. I succeeded in recording at multiple points at more than 10 kHz of time resolution from cytosol of primary cultured neurons derived from mouse, in which GFP or GFP-fused CamKII proteins were expressed, and in analyzing by FCS. I was also successful in recording action potentials from multiple cultured neurons, which enabled to analyze synaptic connections between neuron pairs and also direction of synaptic connections.
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