Linage analysis of neuronal development in zebrafish spinal cord

• Project/Area Number: 23300123
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neuroscience in general
• Research Institution: Okazaki Research Facilities, National Institutes of Natural Sciences
• Principal Investigator: HIGASHIJIMA Shin-ichi 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 准教授 (80270479)
• Project Period (FY): 2011-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥19,890,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥4,590,000); Fiscal Year 2013: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2012: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2011: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
• Keywords: ゼブラフィッシュ / イメージング / 細胞系譜 / 神経分化 / 脊髄

Outline of Final Research Achievements

The developing spinal cord is subdivided into distinct progenitor domains, each of which gives rise to different types of neurons. However, the developmental mechanisms responsible for generating neuronal diversity within a domain are not well understood. Here, we have studied zebrafish V0 neurons, those that derive from the p0 progenitor domain, in order to address this question. We find that all V0 neurons have commissural axons, but they can be divided into excitatory and inhibitory classes. V0 excitatory neurons (V0-e) can be further categorized into three groups based on their axonal trajectories; V0-eA (ascending), V0-eB (bifurcating), and V0-eD (descending) neurons. By using time-lapse imaging of p0 progenitors and their progeny, we show that inhibitory and excitatory neurons are produced from different progenitors. We also demonstrate that V0-eA neurons are produced from distinct progenitors, while V0-eB and V0-eD neurons are produced from common progenitors.

Research Products

• [Journal Article] Efficient generation of knock-in transgenic zebrafish carrying reporter/driver genes by CRISPR/Cas9-mediated genome engineering (2014)
  • Author(s): Kimura, Y., Hisano, Y., Kawahara, A., and Higashijima, S.
  • Journal Title: Scientific Reports Volume: 4 Issue: 1 Pages: 6545-6545
  • DOI: 10.1038/srep06545
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Transgenic tools to characterize neuronal properties of discrete populations of zebrafish neurons (2013)
  • Author(s): Satou, C., Kimura, Y., Hirata, H., Suster, M.L, Kawakami, K., and Higashijima, S.
  • Journal Title: Development Volume: 140 Issue: 18 Pages: 3927-3931
  • DOI: 10.1242/dev.099531
  • Peer Reviewed
• [Journal Article] Hindbrain V2a neurons in the excitation of spinal locomotor circuits during zebrafish swimming (2013)
  • Author(s): Kimura, Y., Satou, C., Fujioka, S., Shoji, W., Umeda, K., Ishizuka, T., Yawo, H., and Higashijima, S.
  • Journal Title: Current Biology Volume: 23 Issue: 10 Pages: 843-849
  • DOI: 10.1016/j.cub.2013.03.066
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Generation of multiple classes of V0 neurons in zebrafish spinal cord: progenitor heterogeneity and temporal control of neuronal diversity. (2012)
  • Author(s): Satou, C., Kimura, Y., and Higashijima, S.
  • Journal Title: J. Neuroscience Volume: 32 Pages: 1771-1783
  • Peer Reviewed
• [Journal Article] Mapping a sensory-motor network onto a structural and functional ground plan in the hindbrain. (2011)
  • Author(s): Koyama, M., Kinkhabwala, A., Satou, C., Higashijima, S., and Fetcho, J.R.
  • Journal Title: Proc. Natl. Acad. Sci. (USA) Volume: 108 Pages: 1170-1175
  • Peer Reviewed
• [Journal Article] A structural and functional ground plan for neurons in the hindbrain of zebrafish. (2011)
  • Author(s): Kinkhabwara, A., Riley, M., Koyama, M., Monen, J., Satou, C., Kimura, Y., Higashijima, S., and Fetcho, J.R.
  • Journal Title: Proc. Natl. Acad. Sci. (USA) Volume: 108 Pages: 1164-1169
  • Peer Reviewed
• [Presentation] Functional analysis of locomotor circuits in the spinal cord and brainstem in zebrafish (2014)
  • Author(s): Higashijima, S.
  • Organizer: International Society for Neuroethology
  • Place of Presentation: 北海道、札幌、札幌コンベンションセンター
  • Year and Date: 2014-07-30
  • Invited
• [Presentation] Functional analysis of locomotor circuits in the spinal cord and brainstem in zebrafish (2013)
  • Author(s): Higashijima, S., Kimura, Y., and Satou, C.
  • Organizer: 第36回日本分子生物学会大会
  • Place of Presentation: 神戸、兵庫県
• [Presentation] Functional analysis of locomotor circuits in the spinal cord and brainstem in zebrafish.
  • Author(s): Higashijima S, Kimura Y, Satou C
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: パシフィコ横浜（神奈川県）