| Project/Area Number |
23300132
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | Osaka University |
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Principal Investigator |
OGURA Akihiko 大阪大学, 生命機能研究科, 教授 (30260631)
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| Co-Investigator(Kenkyū-buntansha) |
TOMINAGA Keiko (YOSHINO Keiko) 大阪大学, 大学院・生命機能研究科, 准教授 (60256196)
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| Co-Investigator(Renkei-kenkyūsha) |
KOJIMA Masami 産業技術総合研究所, 関西センターセルエンジニアリング研究部門, グループ長 (40344171)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2013: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2012: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
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| Keywords | 記憶 / シナプス新生 / シナプス廃止 / BDNF / proBDNF / 脳切片培養 / 記憶固定 / 構造可塑性 / シナプス可塑性 / 長期記憶 / 恐怖学習 / 遺伝子改変マウス / TrkB / p75 / ゆらぎ |
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| Research Abstract |
Brain memory is established through 2 steps; acquisition and consolidation, the cellular mechanisms of the former step have been well analyzed using LTP (long-term potentiation) and LTD (long-term depression) as model phenomena for analysis, while those of the latter remain unclear. We previously reported in the stable cultures of hippocampal slice that 3 repeated inductions of LTP and LTD lead to the long-lasting strengthening and weakening of neuronal circuit supported by new synapse formation and elimination, respectively. In the present research, we demonstrated that the synapse formation is mediated by a BDNF (brain-derived neurotrophic factor)-TrkB signaling pathway, while the synapse elimination is mediated by a proBDNF (precursor protein of BDNF)-p75 pathway. Since BDNF and proBDNF are the products of a single gene, the apparently opposite cellular events are regulated by epigenetic regulation, this fact may provoke a paradigm shift in memory research.
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