Localization of excitatory and inhibitory astrocytes, and their generation mechanism

• Project/Area Number: 23300136
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: National Institute for Physiological Sciences
• Principal Investigator: IKENAKA Kazuhiro 生理学研究所, 分子生理研究系, 教授 (00144527)
• Co-Investigator(Kenkyū-buntansha): FURUYA Kishio 名古屋大学, 工学(系)研究科(研究院), 研究員 (40132740) ; NAMIKI Shigeyuki 東京大学, 医学(系)研究科(研究院), 助教 (90452193)
• Co-Investigator(Renkei-kenkyūsha): TANAKA Kenji 生理学研究所, 分子生理研究系, 助教 (30329700)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥19,890,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥4,590,000); Fiscal Year 2013: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2012: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2011: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
• Keywords: ＡＴＰ / グルタミン酸 / イメージング / アストロサイト / 神経科学 / 薬理学 / ATP

Research Abstract

We succeeded in observing ATP release from astrocytes in brain slices. This release occurred in a region specific manner, suggesting that ATP-releasing astrocytes regulate neural circuit activity. Pharmaceutical analysis indicated that this ATP release from astrocytes induced by the glutamate addition does not depend on the exocytosis but more depends on the release through channels.

Research Products

• [Journal Article] Increased astrocytic ATP release results in enhanced excitability of the hippocampus (2013)
  • Author(s): Lee HU, Yamazaki Y, Tanaka KF, Furuya K, Sokabe M, Hida H, Takao K, Miyakawa T, Fujii S, Ikenaka K
  • Journal Title: Glia Volume: 61巻 Pages: 210-224
  • Peer Reviewed
• [Journal Article] Increased astrocytic ATP release results in enhanced excitability of the hippocampus. (2013)
  • Author(s): 池中 一裕
  • Journal Title: Glia Volume: 61 Pages: 210-224
  • Peer Reviewed
• [Journal Article] Intrinsic and extrinsic mechanisms control the termination of cortical interneuron migration (2012)
  • Author(s): Inamura N, Kimura T, Tada S, Kurahashi T, Yanagida M, Yanagawa Y, Ikenaka K, Murakami F
  • Journal Title: Journal of Neuroscience Volume: 32 Pages: 6032-42
  • Peer Reviewed
• [Journal Article] Geneinduction in mature oligodendorocytes with a PLP-tTA mouse line (2012)
  • Author(s): Inamura N, Sugio S, Macklin WB, Tomita K, Tanaka KF, Ikenaka K
  • Journal Title: Genesis Volume: 50 Pages: 424-8
  • Peer Reviewed
• [Journal Article] Olig2-lineage cells generate GABAergic neurons in the prethalamic nuclei, including the zona incerta (2011)
  • Author(s): Inamura N, Ono K, Takebayashi H, Zalc B, Ikenaka K
  • Journal Title: ventral lateral geniculate nucleus and reticular thalamic nucleus Volume: 33巻 Pages: 118-29
  • Peer Reviewed
• [Journal Article] Olig2-lineage cells generate GABAergic neurons in the prethalamic nuclei, including the zona incerta, ventral lateral geniculate nucleus and reticular thalamic nucleus (2011)
  • Author(s): 池中一裕
  • Journal Title: Developmental Neuroscience Volume: 33 Pages: 118-29
  • Peer Reviewed
• [Journal Article] Intrinsic and extrinsic mechanisms control the termination of cortical interneuron migration
  • Author(s): 池中一裕
  • Journal Title: Journal of Neuroscience Volume: (in press)
  • Peer Reviewed
• [Journal Article] Gene induction in mature oligodendorocytes with a PLP-tTA mouse line
  • Author(s): 池中一裕
  • Journal Title: Genesis Volume: (In press)
  • Peer Reviewed
• [Presentation] Mechanism underlying gliotransmitter release from astrocytes (2013)
  • Author(s): Inamura N
  • Organizer: NIPS-Chulalongkorn Joint Symposium
  • Place of Presentation: Chulalongkorn University Bangkok Thailand
  • Year and Date: 2013-10-21
• [Presentation] Mechanism underlying ATP release from astrocytes (2013)
  • Author(s): Inamura N, Lee HU, Tanaka KF, Furuya K, Sokabe M, Ikenaka K
  • Organizer: Neuro2013
  • Place of Presentation: 京都府 国立京都国際会館
  • Year and Date: 2013-06-20
• [Presentation] Mechanisms underlying gliotransmitter release from astrocytes (2013)
  • Author(s): Inamura N
  • Organizer: NIPS-Chulalongkorn Joint Symposium
  • Place of Presentation: Chulalongkorn University, Bangkok, Thailand
• [Presentation] Mechanisms underlying ATP release from astrocytes (2013)
  • Author(s): Inamura N, Lee HU, Tanaka KF, Furuya K, Sokabe M, Ikenaka K
  • Organizer: Neuro2013
  • Place of Presentation: 国立京都国際会館（京都）
• [Presentation] Mechanism underlying ATP Release From Cultured Astrocyte (2012)
  • Author(s): 池中一裕
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 愛知県名古屋市 名古屋国際会議場
  • Year and Date: 2012-09-20
• [Presentation] Mechanisms underlying ATP release from cultured astrocytes (2012)
  • Author(s): 池中 一裕
  • Organizer: 第３４回日本神経科学大会
  • Place of Presentation: 愛知県名古屋市 名古屋国際会議場
• [Presentation] Mechanism underlying ATP Release From Cultured Astrocyte (2011)
  • Author(s): 池中一裕
  • Organizer: ISN-ESN 2011 23rd Biennial Meeting
  • Place of Presentation: ギリシャ
  • Year and Date: 2011-08-29
• [Presentation] Mechanisms underlying ATP Release From Cultured Astrocyte (2011)
  • Author(s): 池中一裕
  • Organizer: ISN-ESN 2011 23^<rd> Biennial Meeting
  • Place of Presentation: ギリシャ
  • Year and Date: 2011-08-29