Systematic functional mapping of synaptic connections between cerebral cortical areas
| Project/Area Number |
23300148
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Fusional basic brain science
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| Research Institution | National Institute for Basic Biology |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
OKADA Takashi 日本医科大学, 大学院医学研究科, 教授 (00326828)
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| Project Period (FY) |
2011-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥20,020,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥4,620,000)
Fiscal Year 2014: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
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| Keywords | 脳・神経 / 高性能レーザー / 運動野 / 大脳皮質 / 神経科学 |
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| Outline of Final Research Achievements |
We used an in vivo Channelrhodopsin-2 (ChR2) photostimulation method to systematically trace the functional connections between the mouse rostral forelimb area (RFA) and the caudal forelimb area (CFA). Simultaneous electrical recordings were utilized to detect spiking activities induced by synaptic inputs originating from photostimulated areas. This method, in combination with anatomical tracing, demonstrated that the RFA receives strong functional projections from layer 2/3 and/or layer 5a, but not from layer 5b, of the CFA. Further, the CFA receives strong projections from layer 5b neurons of the RFA. Next, we investigated complex movements induced by long-duration ChR2 photostimulation over a broad cortical area of awake mice. Many complex movements including forepaw-to-mouth and locomotion-like movements were mapped in several functional modules in the cerebral cortex. In addition, we developed a method of two-photon calcium imaging in a broad cortical area.
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Report
(5 results)
Research Products
(29 results)
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[Journal Article] Next-generation transgenic mice for optogenetic analysis of neural circuits.2013
Author(s)
Asrican, B, Augustine, G. J., Berglund, K., Chen, S., Chow, N. Deisseroth, K., Feng, G., Gloss, B. Hira, R., Hoffman, C., Kasai, K., Katarya, M.
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Journal Title
Frontier in Neural Circuits
Volume: 7
Pages: 1-10
DOI
Related Report
Peer Reviewed
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[Journal Article] GABA promotes the competitive selection of dendritic spines by controlling local Ca2+ signaling. Nature Neurosci. 16:1409-1416.2013
Author(s)
Hayama, T., Noguchi, J., Watanabe, S., Ellis-Davies, G. C. R., Hayashi, A., Takahashi, N., Matsuzaki, M. & Kasai, H.
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Journal Title
Nature Neurosci.
Volume: 16
Issue: 10
Pages: 1409-1416
DOI
Related Report
Peer Reviewed
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[Journal Article] In vivo two-photon uncaging of glutamate revealing the structure-function relationships o f dendritic spines in the neocortex of adult mice.2011
Author(s)
Noguchi, J., Nagaoka, A., Watanabe, S., Ellis-Davies, G.C.R., Kitamura, K., Kano, M., Matsuzaki, M. & Kasai H.
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Journal Title
J.Physiol.
Volume: 589
Issue: 10
Pages: 2320-2329
DOI
Related Report
Peer Reviewed
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