| Budget Amount *help |
¥20,020,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥4,620,000)
Fiscal Year 2014: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
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| Outline of Final Research Achievements |
We used an in vivo Channelrhodopsin-2 (ChR2) photostimulation method to systematically trace the functional connections between the mouse rostral forelimb area (RFA) and the caudal forelimb area (CFA). Simultaneous electrical recordings were utilized to detect spiking activities induced by synaptic inputs originating from photostimulated areas. This method, in combination with anatomical tracing, demonstrated that the RFA receives strong functional projections from layer 2/3 and/or layer 5a, but not from layer 5b, of the CFA. Further, the CFA receives strong projections from layer 5b neurons of the RFA. Next, we investigated complex movements induced by long-duration ChR2 photostimulation over a broad cortical area of awake mice. Many complex movements including forepaw-to-mouth and locomotion-like movements were mapped in several functional modules in the cerebral cortex. In addition, we developed a method of two-photon calcium imaging in a broad cortical area.
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