Study on the pathogenesis of peripertum cardiomyopathy using genetically modified mouse model
| Project/Area Number |
23300152
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | University of Tsukuba |
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Principal Investigator |
ISHIDA Junji 筑波大学, 生命環境系, 講師 (30323257)
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| Co-Investigator(Renkei-kenkyūsha) |
YAGAMI Ken-ichi 筑波大学, 医学医療系, 教授 (40166476)
SUGIYAMA Fumihiro 筑波大学, 医学医療系, 准教授 (90226481)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥20,540,000 (Direct Cost: ¥15,800,000、Indirect Cost: ¥4,740,000)
Fiscal Year 2013: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2011: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
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| Keywords | 妊娠恒常性 / 周産期心筋症 / APJ受容体 / トランスジェニックマウス / ノックアウトマウス |
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| Research Abstract |
In this study, we established an animal model for "peripertum cardiomyopathy" (MHC-APJ) by cardiomyocyte-specific overexpression of APJ, a member of G-protein coupled receptor superfamily and expressing mainly in cardiovascular system. The lactating MHC-APJ mother exhibited reduced cardiac function, enlargement of the heart, cardiac fibrosis, and enhanced mRNA expression of the marker genes of heart failure. We found that "lactation" is essential for the pathogenesis of above phenotypes in MHC-APJ because those were not observed in non-pregnant or non-lactating MHC-APJ mother. By using an exhaustive gene expression analysis, we identified the several genes that changed only in the heart from lactating MHC-APJ mice. We consider these genes to be the candidates of the pathogenesis of peripertum cardiomyopathy induced by the combined action of enhanced APJ signaling and lactation in mice.
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Report
(4 results)
Research Products
(56 results)
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[Journal Article] Truncated Cables1 causes agenesis of the corpus callosum in mice2014
Author(s)
Mizuno S, Dinh T, Mizobuchi A, Iseki H, Mizuno-Iijima S, Kim J-D, Ishida J, Matsuda Y, Kunita S, Fukamizu A, Yagami K, Sugiyama F
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Journal Title
Laboratory Investigation
Volume: 94
Issue: 3
Pages: 321-330
DOI
Related Report
Peer Reviewed
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[Journal Article] Effects of Aging and Uninephrectomy on Renal Changes in Tsukuba Hypertensive Mice2013
Author(s)
Inui, Y., Mochida H., Yamairi, F., Okada, M., Ishida, J., Fukamizu, A., and Arakawa, K
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Journal Title
Biomed. Reports
Volume: 1
Issue: 3
Pages: 359-364
DOI
Related Report
Peer Reviewed
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[Journal Article] Effect of Lactation on Postpartum Cardiac Function of Pregnancy-Associated Hypertensive Mice2013
Author(s)
Murata, K., Saito, C., Ishida, J., Hamada, J., Sugiyama, F., Yagami, KI., and Fukamizu, A
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Journal Title
Endocrinology
Volume: 154
Issue: 2
Pages: 597-602
DOI
Related Report
Peer Reviewed
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[Journal Article] Mechanism for p38α-mediated experimental autoimmune encephalomyelitis2012
Author(s)
Namiki, K., Matsunaga, H., Yoshioka, K., Tanaka, K., Murata, K., Ishida, J
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Journal Title
J. Biol. Chem
Volume: 287
Issue: 29
Pages: 24228-24238
DOI
Related Report
Peer Reviewed
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[Journal Article] hort-term Suppression of Renin-Angiotensin System in Mice Associated with Hypertension during Pregnancy2012
Author(s)
Ishimaru, T., Ishida, J., Nakamura, S., Hashimoto, M., Matsukura, T., Nakamura, A., Kunita, S., Sugiyama, F., Yagami, K., and Fukamizu, A
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Journal Title
Mol. Med. Reports
Volume: 6
Pages: 28-32
DOI
Related Report
Peer Reviewed
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[Journal Article] Production of free methylarginines via the proteasome and autophagy pathways in cultured cells.2011
Author(s)
Shirakawa, T., Kako,K., Shimada, T., Nagashima, Y., Nakamura, A., Ishida, J.,Fukamizu, A.
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Journal Title
Mol. Med. Rep.
Volume: 4
Pages: 615-620
DOI
Related Report
Peer Reviewed
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