Immunological function and infection control analysis of murine norovirus capsid protein P domain

• Project/Area Number: 23300153
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory animal science
• Research Institution: Jichi Medical University
• Principal Investigator: KUNITA SATOSHI 自治医科大学, 医学部, 教授 (10195472)
• Co-Investigator(Kenkyū-buntansha): YAGAMI Ken-ichi 筑波大学, 医学医療系, 教授 (40166476)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥20,410,000 (Direct Cost: ¥15,700,000、Indirect Cost: ¥4,710,000); Fiscal Year 2013: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2012: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2011: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
• Keywords: マウスノロウイルス / キャプシドタンパク質 / 診断 / 抗体 / 抗原性 / 免疫原性 / 感染阻止 / ノロウイルス / マウス / 血清検査 / 遺伝子検査 / 免疫応答 / ウイルス動態 / VP1タンパク質 / Pドメイン / 血清診断法 / PCR診断法 / 持続感染

Research Abstract

Antibody measurement methods for murine norovirus (MNV) with high specificity and sensitivity were established using the recombinant VP1 protein of MNV. We found that mice can be persistently infected with MNV. The infected mice served as a source of infection for more than one year, and high blood antibody was maintained. Examination of strongly immunogenic region within VP1 protein revealed that VP1-P2 subdomain has the most potent antibody-induced activity. However, a simple approach to induce systemic antibodies to VP1-P2 subdomain could not prevent MNV infection in mice. It may be necessary to induce intestinal immunity such as mucosal IgA to control MNV infection effectively.

Research Products

• [Journal Article] Simultaneous detectionof antibodies to mouse hepatitis virus recombina nt structural proteins by a microsph ere-based multiplex fluorescence imm unoassay (2011)
  • Author(s): Kunita S, Kato K, Ishida M, Hagiw ara K, Kameda S, Ishida T, Takakur a A, Goto K, Sugiyama F, Yagami K
  • Journal Title: Clin Vaccine Immunol Volume: Vol.18, No.5 Pages: 758-766
  • Peer Reviewed
• [Journal Article] Simultaneous detection of antibodies to mouse hepatitis virus recombinant structural proteins by a microsphere-based multiplex fluorescence immunoassay (2011)
  • Author(s): Kunita S, Kato K, Ishida M, Hagiwara K, Kameda S, Ishida T, Takakura A, Goto K, Sugiyama F, Yagami K
  • Journal Title: Clin Vaccine Immunol Volume: 18(5) Issue: 5 Pages: 758-766
  • DOI: 10.1128/cvi.00467-10
  • Peer Reviewed
• [Presentation] マウスノロウイルスの各種抗体検査およびPCR 検査の感度と特異性 (2013)
  • Author(s): 國田智, 加藤花名子, 亀田周子, 石田智子, 林元展人, 高倉彰, 杉山文博, 八神健一
  • Organizer: 第60回日本実験動物学会総会
  • Place of Presentation: つくば
  • Year and Date: 2013-05-15
• [Presentation] マウスノロウイルスの各種抗体検査およびPCR検査の感度と特異性 (2013)
  • Author(s): 國田智、加藤花名子、亀田周子、石田智子、林元展人、高倉彰、杉山文博、八神健一
  • Organizer: 第６０回日本実験動物学会総会
  • Place of Presentation: つくば国際会議場
• [Presentation] マウスノロウイルスの各種抗体検査およびPCR検査の感度と特異性 (2013)
  • Author(s): 國田智，加藤花名子，亀田周子，石田智子，林元展人，高倉彰，杉山文博，八神健一
  • Organizer: 第60回日本実験動物学会総会
  • Place of Presentation: つくば国際会議場
• [Presentation] マウスノロウイルスの抗体検査とPCR 検査の比較評価 (2011)
  • Author(s): 國田智, 加藤花名子, 亀田周子, 石田智子, 林元展人, 高倉彰, 杉山文博, 八神健一
  • Organizer: 第58回日本実験動物学会総会
  • Place of Presentation: 東京
  • Year and Date: 2011-05-26
• [Presentation] マウスノロウイルスの抗体検査とPCR検査の比較評価 (2011)
  • Author(s): 國田智, 加藤花名子, 亀田周子, 石田智子, 林元展人, 高倉彰, 杉山文博, 八神健一
  • Organizer: 第58回日本実験動物学会総会
  • Place of Presentation: タワーホール船堀(東京)
  • Year and Date: 2011-05-26